Molecular identification and characterization of rat Abcc1 cDNA: existence of two splicing variants and species difference in drug-resistance profile

2003 ◽  
Vol 3 (3) ◽  
pp. 136-146 ◽  
Author(s):  
Shin-ichiro Takayanagi ◽  
Toshihisa Ishikawa
Keyword(s):  
Drug Resistance ◽  
Molecular Identification ◽  
Species Difference ◽  
Resistance Profile ◽  
Splicing Variants ◽  
Drug Resistance Profile ◽  
Identification And Characterization

scholarly journals Molecular identification and anti-malarial drug resistance profile of Plasmodium falciparum from patients attending Kisoro Hospital, southwestern Uganda

2022 ◽  
Vol 21 (1) ◽  
Author(s):  
Godfrey Manirakiza ◽  
Kennedy Kassaza ◽  
Ivan Mugisha Taremwa ◽  
Joel Bazira ◽  
Fredrick Byarugaba
Keyword(s):  
Drug Resistance ◽  
Plasmodium Falciparum ◽  
Molecular Identification ◽  
Plasmodium Species ◽  
Pcr Amplification ◽  
Plasmodium Malariae ◽  
Dried Blood Spots ◽  
Resistance Profile ◽  
Drug Resistance Profile ◽  
Southwestern Uganda

Abstract Background The evolution of malaria infection has necessitated the development of highly sensitive diagnostic assays, as well as the use of dried blood spots (DBS) as a potential source of deoxyribonucleic acid (DNA) yield for polymerase chain reaction (PCR) assays. This study identified the different Plasmodium species in malaria-positive patients, and the anti-malarial drug resistance profile for Plasmodium falciparum using DBS samples collected from patients attending Kisoro Hospital in Kisoro district, Southwestern Uganda. Methods The blood samples were prospectively collected from patients diagnosed with malaria to make DBS, which were then used to extract DNA for real-time PCR and high-resolution melting (HRM) analysis. Plasmodium species were identified by comparing the control and test samples using HRM-PCR derivative curves. Plasmodium falciparum chloroquine (CQ) resistance transporter (pfcrt) and kelch13 to screen the samples for anti-malarial resistance markers. The HRM-PCR derivative curve was used to present a summary distribution of the different Plasmodium species as well as the anti-malarial drug profile. Results Of the 152 participants sampled, 98 (64.5%) were females. The average age of the participants was 34.9 years (range: 2 months–81 years). There were 134 samples that showed PCR amplification, confirming the species as Plasmodium. Plasmodium falciparum (N = 122), Plasmodium malariae (N = 6), Plasmodium ovale (N = 4), and Plasmodium vivax (N = 2) were the various Plasmodium species and their proportions. The results showed that 87 (71.3%) of the samples were sensitive strains/wild type (CVMNK), 4 (3.3%) were resistant haplotypes (SVMNT), and 31 (25.4%) were resistant haplotypes (CVIET). Kelch13 C580Y mutation was not detected. Conclusion The community served by Kisoro hospital has a high Plasmodium species burden, according to this study. Plasmodium falciparum was the dominant species, and it has shown that resistance to chloroquine is decreasing in the region. Based on this, molecular identification of Plasmodium species is critical for better clinical management. Besides, DBS is an appropriate medium for DNA preservation and storage for future epidemiological studies.

scholarly journals Identification and Characterization of Multiple Myeloma Stem Cell-Like Cells

Cancers ◽  
2021 ◽  
Vol 13 (14) ◽  
pp. 3523
Author(s):  
Wancheng Guo ◽  
Haiqin Wang ◽  
Peng Chen ◽  
Xiaokai Shen ◽  
Boxin Zhang ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Drug Resistance ◽  
Stem Cell ◽  
New Drugs ◽  
Cell Therapies ◽  
High Relapse Rate ◽  
High Incidence ◽  
Poorly Differentiated ◽  
Identification And Characterization

Multiple myeloma (MM) is a B-cell tumor of the blood system with high incidence and poor prognosis. With a further understanding of the pathogenesis of MM and the bone marrow microenvironment, a variety of adjuvant cell therapies and new drugs have been developed. However, the drug resistance and high relapse rate of MM have not been fundamentally resolved. Studies have shown that, in patients with MM, there is a type of poorly differentiated progenitor cell (MM stem cell-like cells, MMSCs). Although there is no recognized standard for identification and classification, it is confirmed that they are closely related to the drug resistance and relapse of MM. This article therefore systematically summarizes the latest developments in MMSCs with possible markers of MMSCs, introduces the mechanism of how MMSCs work in MM resistance and recurrence, and discusses the active pathways that related to stemness of MM.

scholarly journals Molecular identification and characterization of the tomato flagellin receptor LeFLS2, an orthologue of Arabidopsis FLS2 exhibiting characteristically different perception specificities

2007 ◽  
Vol 64 (5) ◽  
pp. 539-547 ◽  
Author(s):  
Silke Robatzek ◽  
Pascal Bittel ◽  
Delphine Chinchilla ◽  
Petra Köchner ◽  
Georg Felix ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Identification And Characterization

scholarly journals The Drug Resistance Profile of Mycobacterium abscessus Group Strains from Korea

2014 ◽  
Vol 34 (1) ◽  
pp. 31-37 ◽  
Author(s):  
Seung Heon Lee ◽  
Hee Kyung Yoo ◽  
Seol Hee Kim ◽  
Won-Jung Koh ◽  
Chang Ki Kim ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Mycobacterium Abscessus ◽  
Resistance Profile ◽  
Drug Resistance Profile

scholarly journals Molecular identification and characterization of Trichinella spiralis proteasome subunit beta type-7

2015 ◽  
Vol 8 (1) ◽  
pp. 18 ◽  
Author(s):  
Wei Yang ◽  
Ling Li ◽  
Ruo Liu ◽  
Ge Sun ◽  
Chun Liu ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Trichinella Spiralis ◽  
Proteasome Subunit ◽  
Identification And Characterization

scholarly journals In vitro drug-resistance profile in infant acute lymphoblastic leukemia in relation to age, MLL rearrangements and immunophenotype

Leukemia ◽  
2004 ◽  
Vol 18 (3) ◽  
pp. 521-529 ◽  
Author(s):  
N L Ramakers-van Woerden ◽  
H B Beverloo ◽  
A J P Veerman ◽  
B M Camitta ◽  
A H Loonen ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Acute Lymphoblastic Leukemia ◽  
Lymphoblastic Leukemia ◽  
Resistance Profile ◽  
Drug Resistance Profile ◽  
Infant Acute Lymphoblastic Leukemia

scholarly journals First molecular identification and characterization of Spiroplasma citri, the causal agent of citrus stubborn disease in Algerian citrus groves

2019 ◽  
Vol 101 (3) ◽  
pp. 783-783
Author(s):  
Mounira Inas Drais ◽  
Raied Abou Kubaa ◽  
Chahinez Ghezli ◽  
Leonardo Varvaro ◽  
Khaled Djelouah
Keyword(s):  
Molecular Identification ◽  
Causal Agent ◽  
Spiroplasma Citri ◽  
Citrus Groves ◽  
Identification And Characterization ◽  
Citrus Stubborn Disease
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