Short-term tissue response to potential root-end filling materials in infected root canals

2003 ◽  
Vol 30 (4) ◽  
pp. 240-249 ◽  
Author(s):  
B. S. CHONG ◽  
T. R. PITT FORD ◽  
S. P. KARIYAWASAM
1997 ◽  
Vol 30 (2) ◽  
pp. 102-114 ◽  
Author(s):  
B.S. CHONG ◽  
T. R. PITT FORD ◽  
S. P. KARIYAWASAM

2016 ◽  
Vol 695 ◽  
pp. 247-251
Author(s):  
Alexandru Andrei Iliescu ◽  
Cristian Marian Petcu ◽  
Ileana Cristiana Petcu ◽  
Irina Maria Gheorghiu ◽  
Andrei Iliescu ◽  
...  

The retrograde filling is a critical step to a successful outcome of the endodontic surgery. Despite the progress in the technology of novel root-end filling materials, zinc oxide-eugenol cement superEBA is still preserving its clinical value on long-term basis. The study aimed to reconsider the tissue response to the initial irritating effect of this material. Silicon tubes filled with superEBA were subcutaneously implanted for 120 days in white Wistar rats which were afterwards sacrificed. The connective tissue surrounding the superEBA implants revealed fibroblast proliferation and a definite reparatory process without inflammatory reaction. A non-specific tissue healing in progress around the implants, without calcifications, necrosis, and apoptosis was also described after 4 months. SuperEBA proved on animal model that its cytotoxicity is reducing gradually in time until no adverse reaction is observed. The reduced content in eugenol compared to other surgical zinc oxide cements and the benefic effect of o-ethoxybenzoic acid are the support to reconsider SuperEBA as a biocompatible retrograde filling material.


2014 ◽  
Vol 72 (8) ◽  
pp. 673-680 ◽  
Author(s):  
Ulrich Schiffner ◽  
Georg Cachovan ◽  
Jochen Bastian ◽  
Anton Sculean ◽  
Sigrun Eick

Author(s):  
Jader Camilo Pinto ◽  
Fernanda Ferrari Esteves Torres ◽  
Airton Oliveira Santos-Junior ◽  
Marco Antonio Hungaro Duarte ◽  
Juliane Maria Guerreiro-Tanomaru ◽  
...  

Abstract Objective The aim of this study was to investigate the effect of additional apical preparation using the ProDesign Logic (PDL) 50/.01 rotary heat-treated nickel–titanium (NiTi) file with a larger diameter and minimal taper for retreatment of curved root canals. Materials and Methods Mesial curved root canals of 12 mandibular molars were prepared using PDL 25/.06 and filled using the continuous wave of condensation technique and AH Plus sealer. After retreatment using ProDesign S (PDS) 25/.08, PDL 25/.06 and PDL 35/05, a complementary procedure was performed with PDL 50/.01. Microcomputed tomography (micro-CT) scanning was performed before and after retreatment procedures. The cyclic fatigue resistance of unused PDS 25/.08, PDL 25/.06, PDL 35/.05 and PDL 50/.01 instruments (n = 12) was evaluated in a stainless-steel device. Statistical Analysis Data on the volumes of the root canals and the remaining filling materials were submitted to the paired t-test. Cyclic fatigue resistance data was submitted to one-way ANOVA and Tukey’s tests (α = 0.05). Results Use of PDL 50/.01 decreased the remaining filling materials in the apical third (p < 0.05). The root canal volume was similar in the cervical and middle thirds before and after preparation using PDL 50/.01 (p > 0.05). PDL 50/.01 presented the highest cyclic fatigue resistance (p < 0.05). Conclusions Use of the PDL 50/.01 instrument as an additional apical preparation for retreatment of curved root canals improved filling material removal in the apical third, while maintaining the dentin in the cervical and middle thirds. In addition, PDL 50/.01 presented high-flexural resistance.


2003 ◽  
Vol 17 (2) ◽  
pp. 142-146 ◽  
Author(s):  
José Freitas Siqueira Júnior ◽  
Isabela das Neves Rôças

The aim of this study was to describe a 16S rDNA-based nested polymerase chain reaction (nPCR) assay to investigate the occurrence of Campylobacter gracilis in oral infections. Samples were collected from ten infected root canals, ten cases of acute periradicular abscesses and eight cases of adult marginal periodontitis. DNA extracted from the samples was initially amplified using universal 16S rDNA primers. A second round of amplification used the first PCR products to detect C. gracilis using oligonucleotide primers designed from species-specific 16S rDNA signature sequences. The nPCR assay used in this study showed a detection limit of 10 C. gracilis cells and no cross-reactivity was observed with nontarget bacteria. C. gracilis was detected in the three types of oral infections investigated - 4/10 infected root canals; 2/10 acute periradicular abscesses; and 1/8 subgingival specimens from adult periodontitis. The method proposed in this study showed both high sensitivity and high specificity to directly detect C. gracilis in samples from root canal infections, abscesses, and subgingival plaque. Our findings confirmed that C. gracilis may be a member of the microbiota associated with distinct oral infections, and its specific role in such diseases requires further clarification.


2015 ◽  
Vol 17 (2) ◽  
pp. 43
Author(s):  
Rodolfo Zeledón DDS, Esp ◽  
Carolina Ballestero DDS, Esp ◽  
Erick Hernández DDS. Esp ◽  
Tatiana Ramírez DDS, MSD ◽  
Marianela Benavides DDS, Esp ◽  
...  

The objective of this clinical study was to evaluate by polymerase chain reaction (PCR) the antimicrobial efficacy and the outcome of an endodontic protocol treatment (EPT) performed by under-graduated dental students on infected root canals associated with periapical lesions. Fifty-six patients attending for treatment of pulp necrosis and apical periodontitis were included. A specific EPT approach was performed and DNA extracts were taken at the baseline (S1), after a disinfection-neutralization procedure (S2), post-chemomechanical preparation (S3) and after Intracanal medication (S4) for the presence of specific bacteria. The outcome of the EPT was assessed radiographically using the Periapical Index-system (PAI) after 18-month follow-up. An intergroup evaluation shows that when comparing S1 to S3, S1 to S4, S2 to S3, and S2 to S4, the presence of bacteria were significantly reduced (P < 0.05). Finally, S4 was not significantly reduced when compared with S3 (P > 0.05). The most prevalent species in their respect order were Actinomyces Israelii > Enterococcus Faecalis > Fusobacterium Nucleatum/Prevotella Nigrescens > Phorphyromonas Endodontalis. After the 18-month follow-up, the overall success rate of root canal – treated teeth was of 88% (PAI 1 or 2). This In Vivo study demonstrated that EPT significantly reduced the number of cases with positive results for the studied bacteria, showing a profound positive impact in the outcome of endodontic treatment of teeth diagnosed with Pulp Necrosis and Apical Periodontitis. 


2019 ◽  
Vol 5 (2) ◽  
pp. 40
Author(s):  
Renubala Sroa ◽  
Baljeet Sidhu ◽  
Neha Mengi ◽  
Sarbjot Singh ◽  
Maheep Sidhu

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