Microbiological analysis of infected root canals from symptomatic and asymptomatic teeth with periapical periodontitis and the antimicrobial susceptibility of some isolated anaerobic bacteria

The study aimed to investigate the effects of endodontic space decontamination using a laser combined with ozonated water in the therapy of complicated pulp gangrene. The subject of this in vivo study was a 12-year-old patient diagnosed with extensive periapical periodontitis on the mandibular right first molar. Biological samples were initially collected to identify the active pathogen—Enterococcus faecalis, then the nonsurgical endodontic treatment was performed in a single visit, according to the active disinfection guidelines of the identified pathogen. Two-month postoperative, clinical and radiological examination revealed a complete healing of the periapical lesion. The correct diagnosis of this endodontic–periodontal pathology, and the unconventional treatment of the complex system of infected root canals, allowed a favorable treatment result without any surgical intervention. This unconventional approach, which combines a laser technique with ozonated water, allows for predictable results in periapical lesion treatment.

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Microbiological analysis of root canals associated with periapical abscesses (with pain and swelling) and the antimicrobial susceptibility of isolated bacteria

The Pain Clinic ◽

10.1163/156856906778704632 ◽

2006 ◽

Vol 18 (4) ◽

pp. 327-337 ◽

Cited By ~ 1

Author(s):

Selcuk M. Ozbek ◽

M. Sinan Evcil ◽

Ahmet Ozbek ◽

Aziz Sahin Erdoğan ◽

Mustafa Köseoğlu

Keyword(s):

Antimicrobial Susceptibility ◽

Microbiological Analysis ◽

Root Canals

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Cultivable Anaerobic Microbiota of Infected Root Canals

International Journal of Dentistry ◽

10.1155/2012/609689 ◽

2012 ◽

Vol 2012 ◽

pp. 1-5 ◽

Cited By ~ 5

Author(s):

Takuichi Sato ◽

Keiko Yamaki ◽

Naoko Ishida ◽

Kazuhiro Hashimoto ◽

Yasuhisa Takeuchi ◽

...

Keyword(s):

Anaerobic Bacteria ◽

16S Rrna Gene Sequencing ◽

Bacterial Count ◽

Oral Bacteria ◽

Rrna Gene ◽

Anaerobic Culture ◽

Root Canals ◽

Infected Root ◽

Rrna Gene Sequencing ◽

Molecular Biological Techniques

Objective. Periapical periodontitis is an infectious and inflammatory disease of the periapical tissues caused by oral bacteria invading the root canal. In the present study, profiling of the microbiota in infected root canals was performed using anaerobic culture and molecular biological techniques for bacterial identification.Methods. Informed consent was obtained from all subjects (age ranges, 34–71 years). Nine infected root canals with periapical lesions from 7 subjects were included. Samples from infected root canals were collected, followed by anaerobic culture on CDC blood agar plates. After 7 days, colony forming units (CFU) were counted and isolated bacteria were identified by 16S rRNA gene sequencing.Results. The mean bacterial count (CFU) in root canals was(0.5±1.1)×106(range8.0×101–3.1×106), and anaerobic bacteria were predominant (89.8%). The predominant isolates wereOlsenella(25.4%),Mogibacterium(17.7%),Pseudoramibacter(17.7%),Propionibacterium(11.9%) andParvimonas(5.9%).Conclusion. The combination of anaerobic culture and molecular biological techniques makes it possible to analyze rapidly the microbiota in infected root canals. The overwhelming majority of the isolates from infected root canals were found to be anaerobic bacteria, suggesting that the environment in root canals is anaerobic and therefore support the growth of anaerobes.

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Microbiological Analysis of Primary Infected Root Canals with Symptomatic and Asymptomatic Apical Periodontitis of Young Permanent Teeth

Balkan Journal of Dental Medicine ◽

10.2478/bjdm-2020-0027 ◽

2020 ◽

Vol 24 (3) ◽

pp. 44-51

Author(s):

Jelena Marinković ◽

Tatjana Marković ◽

Snežana Brkić ◽

Milena Radunović ◽

Ivan Soldatović ◽

...

Keyword(s):

School Children ◽

Clinical Symptoms ◽

Treatment Strategies ◽

Apical Periodontitis ◽

Permanent Teeth ◽

Microbiological Analysis ◽

Microbial Composition ◽

Root Canals ◽

Facultative Anaerobes ◽

Infected Root

SummaryBackground/Aim: Understanding the composition of bacteria in infected root canals is important for ameliorating the treatment strategies that lead to the elimination of pathogens and infection control, but also prevent reinfection. Aim of this study was to investigate microbial composition of primary infected root canals with apical periodontitis of young permanent teeth, originating form school children in Serbia, and its association with clinical symptoms.Material and Methods: To determine the bacterial composition of infected root canals in children, 35 endodontic samples were obtained. The identification of cultured bacteria was performed by MALDI-TOF MS analysis. The presence or absence of clinical symtoms were recorded.Results: Facultative anaerobes were 2,2 times more frequent than obligate anaerobes. The most common facultative anaerobes belonged to following genera, Streptococcus (58 isolates), Actinomyces (10) and Enterococcus (8), while predominant obligate anaerobes, belonged to genera Veillonella (15), Prevotella (9) and Fusobacterium (8). The most common clinical isolates recovered from infected root canals with symptomatic apical periodontitis were Veillonella parvula (10) and Fusobacterium nucleatum (7), while from the asymptomatic ones, they were Streptococcus mitis/Streptococcus oralis (5). Prevalence of Parvimonas micra, Prevotella buccae and Streptococcus constellatus within the root canals might be associated to clinical symptoms.Conclusions: Species of genera Streptococcus and Veillonella were the most common isolates from primary infected root canals with apical periodontitis in Serbian school children. Facultative anaerobes were predominant over obligate anaerobes. The prevalence of obligate anaerobes was much higher in symptomatic compared to asymptomatic root canal infections. No specific bacterial strain might be associated to a single examined clinical symptom (pain, tenderness to percussion or swelling), but majority of the strains are associated to all of the examined three symptoms.

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Bacteria profile and antibiogram of the bacteria isolated from the exposed pulp of dog canine teeth

Veterinary Record ◽

10.1136/vr.104540 ◽

2018 ◽

Vol 183 (3) ◽

pp. 97-97 ◽

Cited By ~ 2

Author(s):

José Carlos Almansa Ruiz ◽

Annelize Jonker ◽

Anna-Mari Bosman ◽

Gerhard Steenkamp

Keyword(s):

Antimicrobial Susceptibility ◽

Anaerobic Bacteria ◽

Diffusion Test ◽

Gram Positive ◽

Gram Negative ◽

Root Canals ◽

Microbial Species ◽

Facultative Anaerobes

Twenty-seven microbiological samples were taken from root canals (RC) of the canine teeth of 20 dogs where the pulps were non-vital and exposed due to complicated crown fractures. These pulps were cultured for aerobic/anaerobic bacteria. Antimicrobial susceptibility of isolates was determined using the Kirby-Bauer diffusion test. A total of 49 cultivable isolates, belonging to 27 different microbial species and 18 different genera, were recovered from the 27 RCs sampled. Twenty (40.81 per cent) of the cultivable isolates were Gram positive while 29 (59.19 per cent) were Gram negative. Facultative anaerobes were the most common bacteria (77.56 per cent). Aerobic isolates represented 18.36 per cent, and strict anaerobes 4.08 per cent. The antimicrobials with the highest in vitro efficacy were gentamicin (100 per cent) and enrofloxacin (93.32 per cent).

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