Rapid detection and quantification of yeast species during spontaneous wine fermentation by PCR-RFLP analysis of the rDNA ITS region

1999 ◽  
Vol 87 (6) ◽  
pp. 949-956 ◽  
Author(s):  
L. Granchi ◽  
M. Bosco ◽  
A. Messini ◽  
M. Vincenzini
Keyword(s):  
Rapid Detection ◽  
Yeast Species ◽  
Rflp Analysis ◽  
Its Region ◽  
Wine Fermentation ◽  
Rdna Its ◽  
Pcr Rflp ◽  
Rdna Its Region ◽  
Detection And Quantification

scholarly journals PCR-RFLP ITS-based analysis of wine yeast autochthonous strains isolated from different grape cultivars in Taman subregion

2021 ◽  
Vol 285 ◽  
pp. 05020
Author(s):  
Elena Lobodina ◽  
Ivan Suprun ◽  
Natalya Ageeva ◽  
Ekaterina Al-Nakib
Keyword(s):  
Genetic Analysis ◽  
Restriction Analysis ◽  
Its Region ◽  
Wine Yeast ◽  
Product Size ◽  
Rdna Its ◽  
Hanseniaspora Uvarum ◽  
Pcr Rflp ◽  
Autochthonous Yeast ◽  
Rdna Its Region

The studies present the results of morphological, cultural and genetic analysis of the ITS1-ITS4 region of the autochthonous yeast strains genome by using the HaeIII restriction enzyme. On the red and white grapes varieties, based on the morphology of the cells, autochthonous strains belonging to the genus Saccharomyces prevail – 83.3%, what is confirmed by genetic analysis of rDNA ITS region. Restriction analysis showed that all strains of the genus Saccharomyces belong to the species Saccharomyces cerevisiae / S. paradoxus. The percentage of Saccharomyces isolated on the Pervenets Magaracha variety is 86.7%, Krasnostop Anapsky - 80%. The non-Saccharomyces yeast had a product size of 750 bp, presumably of the species Hanseniaspora uvarum.

scholarly journals Brown Ring Patch: A New Disease on Bentgrass Caused by Waitea circinata var. circinata

Plant Disease ◽  
2005 ◽  
Vol 89 (6) ◽  
pp. 536-542 ◽  
Author(s):  
Takeshi Toda ◽  
Tomoyuki Mushika ◽  
Toshihiro Hayakawa ◽  
Akemi Tanaka ◽  
Toshikazu Tani ◽  
...  
Keyword(s):  
Random Amplified Polymorphic Dna ◽  
Rflp Analysis ◽  
Its Region ◽  
Hyphal Growth ◽  
Creeping Bentgrass ◽  
New Disease ◽  
Phylogenic Tree ◽  
Rdna Its ◽  
Rdna Its Region ◽  
Waitea Circinata

Isolates of an unidentified Rhizoctonia sp. (NP isolates), obtained from creeping bentgrass (Agrostis stolonifera var. palustris) in Japan that exhibited symptoms of a new disease, were compared with isolates of three varieties of Waitea circinata var. oryzae, var. zeae, and var. circinata. NP isolates also were compared with isolates of R. oryzae obtained from creeping bent-grass exhibiting white patch-like symptoms (RW isolates). The color and size of sclerotia, color of mycelia, and pigment deposition of NP isolates was similar to that of RW isolates and W. circinata var. circinata, but distinctly different from W. circinata var. oryzae and W. circinata var. zeae. The optimal temperature for hyphal growth of NP isolates, RW isolates, and W. circinata var. circinata was 28°C, and for W. circinata var. oryzae and W. circinata var. zeae was 30°C. Pathogenicity tests on creeping bentgrass showed that the severity of disease caused by NP isolates, RW isolates, and W. circinata var. circinata was greater than with W. circinata var. oryzae, but lower than with W. circinata var. zeae. No significant differences in symptom expression were apparent among NP isolates, RW isolates, and W. circinata var. circinata. A phylogenic tree, obtained using the results of random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR), showed that isolates of W. circinata var. oryzae and W. circinata var. zeae separated into individual clusters, while NP isolates, RW isolates, and W. circinata var. circinata clustered together. The lengths of the rDNA internal transcribed spacer (ITS) region of NP isolates, RW isolates, and W. circinata var. circinata were identical but smaller than those of W. circinata var. oryzae and W. circinata var. zeae. Restriction fragment length polymorphism (RFLP) analysis of the rDNA-ITS region, using three enzymes (HapII, HinfI, and MboI), also showed that NP isolates were the same as RW isolates and W. circinata var. circinata, but different from W. circinata var. oryzae and W. circinata var. zeae. Based on these results, the NP isolates causing a new disease on bentgrass are W. circinata var. circinata, and that RW isolates are also W. circinata var. circinata but not R. oryzae. We propose that the name of the disease on creeping bentgrass caused by W. circinata var. circinata is brown ring patch.

Steinernema apuliae sp. n. (Rhabditida: Steinernematidae): a new entomopathogenic nematode from southern Italy

Zootaxa ◽  
2004 ◽  
Vol 460 (1) ◽  
pp. 1 ◽  
Author(s):  
ORESTE TRIGGIANI ◽  
ZDENEK MRÁ»EK ◽  
ALEX REID
Keyword(s):  
First Generation ◽  
Southern Italy ◽  
Entomopathogenic Nematode ◽  
Rflp Analysis ◽  
Tail Length ◽  
Its Region ◽  
The Body ◽  
Steinernema Glaseri ◽  
Pcr Rflp ◽  
H 41

Steinernema apuliae sp. n. has been found in soil samples collected along a saltpan border habitat in southern Italy characterized by a salted silt soil. This species belongs to the long-IJ nematode group represented by Steinernema glaseri (Steiner, 1929) and Steinernema arenarium (Artyukhovsky, 1967) among others. However, it differs from these taxa in some morphometric values such as V%, H%. Females possess asymmetrical, oblique slit vulva, slant vagina and small flap in the vulval opening. These characteristics are more distinct in second generation females which is different from most other steinernematids; the vulva position is behind the mid-body about 57% to 61% of the body length. First-generation females have a conical-like tip bearing 2 to 3 papilla-like protuberances. Male mucron is absent in both generations. Lightly brown spicules have bluntly pointed tip and elongated manubrium. Third-stage infective juveniles are on average over 1000 m long; the position of the excretory pore is posterior (D% 66) and the hyaline layer is less than half the tail length (H% 41 42). Lateral fields are formed by 8 equally distributed ridges. S. apuliae differs from S. glaseri and S. arenarium and is separated by PCR-RFLP analysis of the ITS region. There were no positive cross-breedings among these species.

scholarly journals Phylogenetic Analysis and Molecular Diversity of Capsicum Based on rDNA-ITS Region

Horticulturae ◽  
2020 ◽  
Vol 6 (4) ◽  
pp. 87
Author(s):  
Kumpei Shiragaki ◽  
Shuji Yokoi ◽  
Takahiro Tezuka
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Analysis ◽  
Wild Species ◽  
Morphological Characteristics ◽  
Its Region ◽  
Internal Transcribed Spacers ◽  
Nuclear Ribosomal Dna ◽  
Rdna Its ◽  
Rdna Its Region ◽  
Genus Capsicum

The genus Capsicum is comprised of 5 domesticated and more than 30 wild species. The region of nuclear ribosomal DNA internal transcribed spacers (rDNA-ITS) has widely been used for species identification, but has rarely been used in Capsicum. In this study, the evaluation of genetic diversity and a phylogenetic analysis were conducted using rDNA-ITS of 28 Capsicum accessions, including five domesticated and two wild species. We surveyed six conventional keys of domesticated species and another five traits in Capsicum accessions. Specific morphological characteristics were found in C. annuum, C. baccatum, and C.pubescens. Three subclones of each accession were sequenced, and rDNA-ITS polymorphisms were detected in all accessions excluding C. annuum, suggesting that incomplete concerted evolution occurred in rDNA-ITS of Capsicum. The genetic diversity was evaluated using nucleotide polymorphism and diversity. C. annuum had the lowest genetic diversity of all species in this study. The phylogenetic tree formed a species-specific clade for C. annuum, C. baccatum, and C. pubescens. The C. chinense clade existed in the C. frutescens clade, implying that it was a cultivated variant of C. frutescens. C. chacoense likely belonged to the C. baccatum complex according to its morphologic and genetic features. This study indicated that the rDNA-ITS region can be used for simple identification of domesticated Capsicum species.

scholarly journals Molecular identification and artificial cultivation of a wild isolate of oyster mushroom in Albania

2016 ◽  
Vol 10 (1s) ◽  
pp. 35
Author(s):  
Jordan Merkuri ◽  
Stefania Mirela Mang ◽  
Ippolito Camele ◽  
Magdalena Cara ◽  
Gian Luigi Rana
Keyword(s):  
Its Region ◽  
Edible Mushroom ◽  
Biological Efficiency ◽  
Potato Dextrose Agar Medium ◽  
Wild Isolate ◽  
Rdna Its ◽  
Pure Cultures ◽  
Rdna Its Region ◽  
And Diffusion ◽  
Artificial Cultivation

Basidiomata of a wild mushroom macroscopically recognised as <em>Pleurotus ostreatus</em> were observed on an oak trunk in a mixed wood of northern Albania. Pure cultures of the fungus were then obtained on potato-dextrose-agar medium. Molecular analyses of genomic DNA of the fungus confirmed its identification. The rDNA ITS region nucleotide sequence of the studied <em>Pleurotacea</em> matched at 99% those of two <em>P. ostreatus</em> strains already present in NCBI GenBank database. The rDNA ITS nucelotide sequences of two pure cultures of the Albanian <em>P. ostreatus</em> were deposited in EMBL database under the accession numbers LN849458 and LN849459. One of the fungus isolates was subsequently cultivated under protected and semi-natural conditions. Productivity and biological efficiency of the Albanian <em>P. ostreatus</em> ranged from about 10% to 16% and from 33 to 53.33%, respectively. This seems to be the first report on the artificial cultivation of <em>P. ostreatus</em> in Albania and could have, in the next future, a high economic impact on development and diffusion of this important edible mushroom over the country.

scholarly journals Molecular Genetic Characterization of Olpidium virulentus Isolates Associated with Big-Vein Diseased Lettuce Plants

Plant Disease ◽  
2010 ◽  
Vol 94 (5) ◽  
pp. 563-569 ◽  
Author(s):  
L. D. Maccarone ◽  
M. J. Barbetti ◽  
K. Sivasithamparam ◽  
R. A. C. Jones
Keyword(s):  
Molecular Genetic ◽  
Leaf Tissue ◽  
Its Region ◽  
Metropolitan Region ◽  
Tissue Samples ◽  
Reverse Transcription Pcr ◽  
Rdna Its ◽  
Australian Continent ◽  
Rdna Its Region ◽  
Southwest Australia

Lettuce plants showing symptoms of lettuce big-vein disease were collected from fields in the Perth Metropolitan region of southwest Australia. When root extracts from each plant were tested by polymerase chain reaction (PCR) using primers specific to the rDNA internal transcribed spacer (ITS) region of Olpidium brassicae and O. virulentus, only O. virulentus was detected in each of them. The nucleotide sequences of the complete rDNA ITS regions of isolates from five of the root samples and 10 isolates of O. virulentus from Europe and Japan showed 97.9 to 100% identities. However, with the six O. brassicae isolates, their identities were only 76.9 to 79.4%. On phylogenetic analysis of the complete rDNA-ITS region sequences of the five Australian isolates and 10 others, the Australian isolates fitted within two clades of O. virulentus (I and II), and within clade I into two of its four subclades (Ia and Id). Japanese isolates had greatest sequence diversity fitting into both clades and into all of clade I subclades except Ib, while European isolates were restricted to subclades Ib and Id. When the partial rDNA-ITS region sequences of two additional southwest Australian isolates, four from Europe, and four from the Americas were included in the analyses, the Australian isolates were within O. virulentus subclades Ia and Id, the European isolates within subclade Ic, and the American isolates within subclades Ia and Ib. These findings suggest that there may have been at least three separate introductions of O. virulentus into the isolated Australian continent since plant cultivation was introduced following its colonization by Europeans. They also have implications regarding numbers of different introductions to other isolated regions. Lettuce big-vein associated virus and Mirafiori lettuce big-vein virus were both detected when symptomatic lettuce leaf tissue samples corresponding to the root samples from southwest Australia were tested using virus-specific primers in reverse transcription–PCR, so presence of both viruses was associated with O. virulentus occurrence.

scholarly journals Deep-level diagnostic value of the rDNA-ITS region

1996 ◽  
Vol 13 (9) ◽  
pp. 1276-1295 ◽  
Author(s):  
M. A. Hershkovitz ◽  
L. A. Lewis
Keyword(s):  
Deep Level ◽  
Its Region ◽  
Diagnostic Value ◽  
Rdna Its ◽  
Rdna Its Region
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