scholarly journals Specific variations of fatty acid composition of Pseudomonas aeruginosa ATCC 15442 induced by Quaternary Ammonium Compounds and relation with resistance to bactericidal activity

1999 ◽  
Vol 87 (5) ◽  
pp. 735-742 ◽  
Author(s):  
L. Guerin-Mechin ◽  
F. Dubois-Brissonnet ◽  
B. Heyd ◽  
J. Y. Leveau
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Bactericidal Activity ◽  
Quaternary Ammonium ◽  
Quaternary Ammonium Compounds ◽  
Ammonium Compounds

scholarly journals Adaptation of Pseudomonas aeruginosa to constant sub-inhibitory concentrations of quaternary ammonium compounds

2020 ◽  
Vol 6 (4) ◽  
pp. 1139-1152 ◽  
Author(s):  
Margaux Voumard ◽  
Leonardo Venturelli ◽  
Myriam Borgatta ◽  
Antony Croxatto ◽  
Sandor Kasas ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Quaternary Ammonium ◽  
Quaternary Ammonium Compounds ◽  
Membrane Properties ◽  
Ammonium Compounds

The susceptibility profile of P. aeruginosa exposed to constant sub-inhibitory 3 concentrations of quaternary ammonium compounds was characterized as well as the 4 nanomechanical membrane properties.

Changes in Membrane Fatty Acid Composition of Pseudomonas aeruginosa in Response to UV-C Radiations

2013 ◽  
Vol 67 (1) ◽  
pp. 112-117 ◽  
Author(s):  
Salma Kloula Ben Ghorbal ◽  
Abdelwaheb Chatti ◽  
Mohamed Marwan Sethom ◽  
Lobna Maalej ◽  
Mouadh Mihoub ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Membrane Fatty Acid ◽  
Membrane Fatty Acid Composition ◽  
Uv C

scholarly journals Quaternary ammonium compounds. A new technique for the study of their bactericidal action and the results obtained with cetavlon (Cetyltrimethylammonium bromide)

1949 ◽  
Vol 47 (3) ◽  
pp. 271-277 ◽  
Author(s):  
G. E. Davies
Keyword(s):  
Organic Matter ◽  
Bactericidal Activity ◽  
Quaternary Ammonium ◽  
Cetyltrimethylammonium Bromide ◽  
Room Temperature ◽  
Quaternary Ammonium Compounds ◽  
Bactericidal Action ◽  
A New Technique ◽  
Suitable Technique ◽  
Ammonium Compounds

1. Some sources of error in the testing of quaternary ammonium compounds for bactericidal activity have been investigated. These resolve themselves into (a) errors caused by bacteriostasis, and (b) errors caused by the clumping of bacteria.2. A suitable technique, free from these errors, is described.3. Cetavlon in 0·1% solution is a powerful bactericide even in the presence of moderate amounts of organic matter.4. One per cent Cetavlon will not kill spores at room temperature even when contact is maintained for several days.5. Bacteria surviving the action of quaternary ammonium compounds retain their virulence.

scholarly journals Impact of Bacterial Membrane Fatty Acid Composition on the Failure of Daptomycin To KillStaphylococcus aureus

2018 ◽  
Vol 62 (7) ◽  
Author(s):  
Rym Boudjemaa ◽  
Clément Cabriel ◽  
Florence Dubois-Brissonnet ◽  
Nicolas Bourg ◽  
Guillaume Dupuis ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Treatment Failure ◽  
Acid Composition ◽  
Bactericidal Activity ◽  
Membrane Fatty Acid ◽  
Membrane Composition ◽  
Membrane Fatty Acid Composition ◽  
Content Type

ABSTRACTDaptomycin is a last-resort membrane-targeting lipopeptide approved for the treatment of drug-resistant staphylococcal infections, such as bacteremia and implant-related infections. Although cases of resistance to this antibiotic are rare, increasing numbers of clinical,in vitro, and animal studies report treatment failure, notably againstStaphylococcus aureus. The aim of this study was to identify the features of daptomycin and its target bacteria that lead to daptomycin treatment failure. We show that daptomycin bactericidal activity againstS. aureusvaries significantly with the growth state and strain, according to the membrane fatty acid composition. Daptomycin efficacy as an antibiotic relies on its ability to oligomerize within membranes and form pores that subsequently lead to cell death. Our findings ascertain that daptomycin interacts with tolerant bacteria and reaches its membrane target, regardless of its bactericidal activity. However, the final step of pore formation does not occur in cells that are daptomycin tolerant, strongly suggesting that it is incapable of oligomerization. Importantly, membrane fatty acid contents correlated with poor daptomycin bactericidal activity, which could be manipulated by fatty acid addition. In conclusion, daptomycin failure to treatS. aureusis not due to a lack of antibiotic-target interaction, but is driven by its capacity to form pores, which depends on membrane composition. Manipulation of membrane fluidity to restoreS. aureusdaptomycin bactericidal activityin vivocould open the way to novel antibiotic treatment strategies.

A COMPARISON OF SOME PROPERTIES OF STRAINS OF PSEUDOMONAS AERUGINOSA SENSITIVE AND RESISTANT TO QUATERNARY AMMONIUM COMPOUNDS

1958 ◽  
Vol 4 (5) ◽  
pp. 499-503 ◽  
Author(s):  
D. R. MacGregor ◽  
P. R. Elliker
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Ammonium Chloride ◽  
Yeast Extract ◽  
Quaternary Ammonium ◽  
Substrate Oxidation ◽  
Quaternary Ammonium Compounds ◽  
Extract Agar ◽  
Resistant Strains ◽  
Ammonium Compounds ◽  
Yeast Extract Agar

Pseudomonas aeruginosa was adapted to grow on tryptone – glucose – yeast extract agar containing 2000 p.p.m. alkyldimethylethylbenzyl ammonium chloride (QAC-A). In germicidal trials these resistant cells could be made QAC-A sensitive by treatment with ethylenediaminetetraacetate. In Warburg studies lyophilized preparations of resistant strains were more sensitive to inhibition of substrate oxidation by QAC-A than the normal strain.It is postulated that quaternary ammonium germicide resistant strains of P. aeruginosa owe their resistance to their impermeability to QAC.

scholarly journals Correlation between Resistance of Pseudomonas aeruginosa to Quaternary Ammonium Compounds and Expression of Outer Membrane Protein OprR

2003 ◽  
Vol 47 (7) ◽  
pp. 2093-2099 ◽  
Author(s):  
Atsushi Tabata ◽  
Hideaki Nagamune ◽  
Takuya Maeda ◽  
Keiji Murakami ◽  
Yoichiro Miyake ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Quaternary Ammonium ◽  
Resistant Strain ◽  
Quaternary Ammonium Compounds ◽  
Cross Resistance ◽  
Knockout Mutant ◽  
Ammonium Compounds

ABSTRACT The adaptation mechanism of Pseudomonas aeruginosa ATCC 10145 to quaternary ammonium compounds (QACs) was investigated. A P. aeruginosa strain with adapted resistance to QACs was developed by a standard broth dilution method. It was revealed that P. aeruginosa exhibited remarkable resistance to N-dodecylpyridinium iodide (P-12), whose structure is similar to that of a common disinfectant, cetylpyridinium chloride. Adapted resistance to benzalkonium chloride (BAC), which is commonly used as a disinfectant, was also observed in P. aeruginosa. Moreover, the P-12-resistant strain exhibited cross-resistance to BAC. Analysis of the outer membrane protein of the P-12-resistant strain by two-dimensional polyacrylamide gel electrophoresis showed a significant increase in the level of expression of a protein (named OprR) whose molecular mass was approximately 26 kDa. The actual function of OprR is not yet clear; however, OprR was expected to be an outer membrane-associated protein with homology to lipoproteins of other bacterial species, according to a search of the National Center for Biotechnology Information website with the BLAST program by use of the N-terminal sequence of OprR. A correlation between the level of expression of OprR and the level of resistance of P. aeruginosa to QACs was observed by using a PA2800 gene knockout mutant derived from the P-12-resistant strain. The knockout mutant recovered susceptibility not only to P-12 but also to BAC. These results suggested that OprR significantly participated in the adaptation of P. aeruginosa to QACs, such as P-12 and BAC.

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