Quaternary ammonium compound stresses induce specific variations in fatty acid composition of Pseudomonas aeruginosa

2000 ◽  
Vol 55 (1-3) ◽  
pp. 157-159 ◽  
Author(s):  
L Guerin-Mechin ◽  
F Dubois-Brissonnet ◽  
B Heyd ◽  
J.Y Leveau
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Quaternary Ammonium ◽  
Quaternary Ammonium Compound ◽  
Ammonium Compound

Changes in Membrane Fatty Acid Composition of Pseudomonas aeruginosa in Response to UV-C Radiations

2013 ◽  
Vol 67 (1) ◽  
pp. 112-117 ◽  
Author(s):  
Salma Kloula Ben Ghorbal ◽  
Abdelwaheb Chatti ◽  
Mohamed Marwan Sethom ◽  
Lobna Maalej ◽  
Mouadh Mihoub ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Membrane Fatty Acid ◽  
Membrane Fatty Acid Composition ◽  
Uv C

A Strain of Pseudomonas aeruginosa Resistant to a Quaternary Ammonium Compound1

1976 ◽  
Vol 39 (8) ◽  
pp. 546-550
Author(s):  
C. J. WASHAM ◽  
W. E. SANDINE ◽  
P. R. ELLIKER
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Quaternary Ammonium ◽  
Inclusion Bodies ◽  
Light And Electron Microscopy ◽  
Quaternary Ammonium Compound ◽  
Ammonium Compound ◽  
Dense Inclusion ◽  
Yeast Extract Agar ◽  
Electron Dense Inclusion ◽  
Resistant Cells

Light and electron microscopy studies were made of Pseudomonas aeruginosa strains which were sensitive and resistant to a quaternary ammonium compound (QAC). The colonies of the sensitive cells on Tryptone Glucose Yeast Extract Agar were granular and homogeneous in consistency. In contrast, the colonies of the resistant strain on the same medium were granular, non-homogenous, and contained numerous dense areas. Morphological observations revealed the resistant cells to be 30% smaller than sensitive cells and non-motile due to loss of polar flagella, a characteristic which was not restored when the organisms were cultured in the absence of QAC for more than 7 months. Electron-dense inclusion bodies were present in resistant cells; they ranged in size from about 0.05 to 0.2 μm in diameter. These bodies, which were not identified, were released intact from lysing cells; as many as 20 per cell were visible.

scholarly journals  Biofilm formation by Pseudomonas aeruginosa and disinfectant susceptibility of planktonic and biofilm cells

2016 ◽  
Vol 34 (No. 3) ◽  
pp. 204-210 ◽  
Author(s):  
Olszewska Magdalena A ◽  
Kocot Aleksandra M ◽  
Stanowicka Aleksandra ◽  
Łaniewska-Trokenheim Łucja
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Quaternary Ammonium ◽  
Biofilm Formation ◽  
Membrane Integrity ◽  
Quaternary Ammonium Compound ◽  
Epifluorescence Microscopy ◽  
Ammonium Compound ◽  
Cell Membrane Integrity ◽  
Propidium Iodide Staining ◽  
Chamber Slide

Epifluorescence microscopy (EFM) was used to study the biofilm formation of Pseudomonas aeruginosa after 6, 24, 30, 48, 54, 72, 78, and 96 h growth in a chamber slide system. For this purpose, the biofilm was stained with the Live/Dead BacLight, wherein live and dead cells were visualised based on the cell membrane integrity. With the use of EFM we described 8- of 9-stage biofilm characteristics after 78 h of growth, since the majority of microscopic fields were fully covered with attached cells. However, the 96-h growth resulted in the cell detachment and revealed 30% of dead cells of all those cells that remained on the surface. The susceptibility testing of planktonic and biofilm cells to two disinfectants, chlorine-based and quaternary ammonium compound-based, revealed that biofilm cells were more tolerant to a chlorine-based sanitiser than planktonic counterparts. P. aeruginosa was inhibited by lower concentrations of the quaternary ammonium compound-based sanitiser than the chlorine-based sanitiser, which on the other hand was more effective in cell inactivation, as both the MIC/MBC (inhibitory/bactericidal) measurement and the CFDA/PI (carboxyfluorescein diacetate/propidium iodide) staining indicated.

Characterization of Pseudomonas aeruginosa Resistant to a Quaternary Ammonium Compound.

2002 ◽  
Vol 7 (3) ◽  
pp. 147-153 ◽  
Author(s):  
ATSUSHI TABATA ◽  
TAKUYA MAEDA ◽  
HIDEAKI NAGAMUNE ◽  
HIROKI KOURAI
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Quaternary Ammonium ◽  
Quaternary Ammonium Compound ◽  
Ammonium Compound

A Strain of Pseudomonas aeruginosa Resistant to a Quaternary Ammonium Compound

1976 ◽  
Vol 39 (4) ◽  
pp. 273-279 ◽  
Author(s):  
C. J. WASHAM ◽  
W. E. SANDINE ◽  
P. R. ELLIKER
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Yeast Extract ◽  
Quaternary Ammonium ◽  
Cell Types ◽  
Quaternary Ammonium Compound ◽  
Ammonium Compound ◽  
Ph Range ◽  
Second Peak ◽  
Precise Degree ◽  
Resistant Cells

Cells of Pseudomonas aeruginosa able to grow in Tryptone glucose yeast extract (TGY) broth containing 750 μg/ml of quaternary ammonium compound (alkyldimethylethylbenzyl ammonium chloride-(QAC) were studied in comparison to sensitive cells unable to grow in such medium to identify factors important in determining the precise degree of resistance. The germicidal activity of QAC solutions against both sensitive and resistant cells in TGY broth was shown to be greatly affected by the concentration of Tryptone and yeast extract, but not by the amount of sugar. The pH of the broth also influenced germicidal activity; both strains were more susceptible under slightly acid conditions. A comparison of the pH range of growth of sensitive and resistant cells demonstrated the abiility of the former to grow in TGY broth at pH 4.5 while the latter could not achieve growth at pH 5.0. A study of the effects of 50 ppm QAC, buffered to various pH values, indicated that susceptibility of resistant cells was nearly the same as that of sensitive cells below pH 3.0, at pH 6.0, and above pH 9.0. The greatest difference between the two cell types occurred from pH 3.5 to pH 4.5 with a second peak of resistance being observed from pH 7.0 to pH 8.5.

Platelet Aggregation in Finnish Men and Its Relation to Fatty Acids in Platelets, Plasma and Adipose Tissue

1985 ◽  
Vol 54 (03) ◽  
pp. 563-569 ◽  
Author(s):  
M K Salo ◽  
E Vartiainen ◽  
P Puska ◽  
T Nikkari
Keyword(s):  
Fatty Acids ◽  
Adipose Tissue ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Platelet Aggregation ◽  
Acid Composition ◽  
Saturated Fatty Acids ◽  
Free Living ◽  
Ω3 Fatty Acids ◽  
Induced Aggregation

SummaryPlatelet aggregation and its relation to fatty acid composition of platelets, plasma and adipose tissue was determined in 196 randomly selected, free-living, 40-49-year-old men in two regions of Finland (east and southwest) with a nearly twofold difference in the IHD rate.There were no significant east-southwest differences in platelet aggregation induced with ADP, thrombin or epinephrine. ADP-induced platelet secondary aggregation showed significant negative associations with all C20-C22 ω3-fatty acids in platelets (r = -0.26 - -0.40) and with the platelet 20: 5ω3/20: 4ω 6 and ω3/ ω6 ratios, but significant positive correlations with the contents of 18:2 in adipose tissue (r = 0.20) and plasma triglycerides (TG) (r = 0.29). Epinephrine-induced aggregation correlated negatively with 20: 5ω 3 in plasma cholesteryl esters (CE) (r = -0.23) and TG (r = -0.29), and positively with the total percentage of saturated fatty acids in platelets (r = 0.33), but had no significant correlations with any of the ω6-fatty acids. Thrombin-induced aggregation correlated negatively with the ω3/6ω ratio in adipose tissue (r = -0.25) and the 20: 3ω6/20: 4ω 6 ratio in plasma CE (r = -0.27) and free fatty acids (FFA) (r = -0.23), and positively with adipose tissue 18:2 (r = 0.23) and 20:4ω6 (r = 0.22) in plasma phospholipids (PL).The percentages of prostanoid precursors in platelet lipids, i. e. 20: 3ω 6, 20: 4ω 6 and 20 :5ω 3, correlated best with the same fatty acids in plasma CE (r = 0.32 - 0.77) and PL (r = 0.28 - 0.74). Platelet 20: 5ω 3 had highly significant negative correlations with the percentage of 18:2 in adipose tissue and all plasma lipid fractions (r = -0.35 - -0.44).These results suggest that, among a free-living population, relatively small changes in the fatty acid composition of plasma and platelets may be reflected in significant differences in platelet aggregation, and that an increase in linoleate-rich vegetable fat in the diet may not affect platelet function favourably unless it is accompanied by an adequate supply of ω3 fatty acids.

Health Benefits of Pumpkin Seed and Nutrition Profile of 35 Pumpkin Accessions

EDIS ◽  
2017 ◽  
Vol 2017 (6) ◽  
Author(s):  
Geoffrey M. Meru ◽  
Yuqing Fu ◽  
Dayana Leyva ◽  
Paul Sarnoski ◽  
Yavuz Yagiz
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Health Benefits ◽  
Pumpkin Seed ◽  
Production Practices

This article aims to summise production and nutrition aspects of pumpkin seed. Specifically, it focuses on health benefits of the seeds, production practices and provides data on the oil, protein and fatty acid composition of 35 pumpkin accessions.  

Sign in / Sign up

Export Citation Format

Share Document