Shiga-toxin-producing Escherichia coli in faeces of healthy dairy cows, sheep and goats: prevalence and virulence properties

ABSTRACTThe main pathogenic enterohemorrhagicEscherichia coli(EHEC) strains are defined as Shiga toxin (Stx)-producingE. coli(STEC) belonging to one of the following serotypes: O157:H7, O26:H11, O103:H2, O111:H8, and O145:H28. Each of these five serotypes is known to be associated with a specific subtype of the intimin-encoding gene (eae). The objective of this study was to evaluate the prevalence of bovine carriers of these “top five” STEC in the four adult cattle categories slaughtered in France. Fecal samples were collected from 1,318 cattle, including 291 young dairy bulls, 296 young beef bulls, 337 dairy cows, and 394 beef cows. A total of 96E. coliisolates, including 33 top five STEC and 63 atypical enteropathogenicE. coli(aEPEC) isolates, with the same genetic characteristics as the top five STEC strains except that they lacked anstxgene, were recovered from these samples. O157:H7 was the most frequently isolated STEC serotype. The prevalence of top five STEC (all serotypes included) was 4.5% in young dairy bulls, 2.4% in young beef bulls, 1.8% in dairy cows, and 1.0% in beef cows. It was significantly higher in young dairy bulls (P< 0.05) than in the other 3 categories. The basis for these differences between categories remains to be elucidated. Moreover, simultaneous carriage of STEC O26:H11 and STEC O103:H2 was detected in one young dairy bull. Lastly, the prevalence of bovine carriers of the top five STEC, evaluated through a weighted arithmetic mean of the prevalence by categories, was estimated to 1.8% in slaughtered adult cattle in France.

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Virulence Properties and Serotypes of Shiga Toxin-Producing Escherichia coli from Healthy Australian Slaughter-Age Sheep

Journal of Clinical Microbiology ◽

10.1128/jcm.39.5.2017-2021.2001 ◽

2001 ◽

Vol 39 (5) ◽

pp. 2017-2021 ◽

Cited By ~ 50

Author(s):

S. P. Djordjevic ◽

M. A. Hornitzky ◽

G. Bailey ◽

P. Gill ◽

B. Vanselow ◽

...

Keyword(s):

Escherichia Coli ◽

Shiga Toxin ◽

Slaughter Age ◽

Virulence Properties

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Virulence Properties of Shiga Toxin-Producing Escherichia coli (STEC) Strains of Serogroup O118, a Major Group of STEC Pathogens in Calves

Journal of Clinical Microbiology ◽

10.1128/jcm.36.6.1604-1607.1998 ◽

1998 ◽

Vol 36 (6) ◽

pp. 1604-1607 ◽

Cited By ~ 35

Author(s):

L. H. Wieler ◽

Anja Schwanitz ◽

Elke Vieler ◽

Barbara Busse ◽

H. Steinrück ◽

...

Keyword(s):

Escherichia Coli ◽

Shiga Toxin ◽

Diagnostic Value ◽

Adhesive Properties ◽

E Coli ◽

Virulence Potential ◽

High Prevalence ◽

Enterocyte Effacement ◽

Diarrheic Calves ◽

Virulence Properties

Shiga toxin-producing Escherichia coli (STEC) strains of serogroup O118 are the most prevalent group among STEC strains in diarrheic calves in Germany (L. H. Wieler, Ph.D. thesis, University of Giessen, 1997). To define their virulence properties, 42 O118 (O118:H16 [n = 38] and O118:H− [n = 4]) strains were characterized. The strains displayed three different Stx combinations (Stx1 [36 of 42], Stx1 and Stx2 [2 of 42], and Stx2 [4 of 42]). A total of 41 strains (97.6%) harbored a large virulence-associated plasmid containinghly EHEC (hly from enterohemorrhagicE. coli). The strains’ adhesive properties varied in relation to the eukaryotic cells tested. Only 28 of 42 strains (66.7%) showed localized adhesion (LA) in the human HEp-2 cell line. In contrast, in bovine fetal calf lung (FCL) cells, the number of LA-positive strains was much higher (37 of 42 [88.1%]). The locus of enterocyte effacement (LEE) was detected in 41 strains (97.6%). However, not all LEE-positive strains reacted positively in the fluorescence actin-staining (FAS) test, which indicated the attaching and effacing (AE) lesion. In HEp-2 cells, only 22 strains (52.4%) were FAS positive, while in FCL cells, the number of FAS-positive strains was significantly higher (38 of 42 [90.5%; P < 0.001]). In conclusion, the vast majority of the O118 STEC strains from calves (41 of 42 [97.6%]) have a high virulence potential (stx, hly EHEC, and LEE). This virulence potential and the high prevalence of STEC O118 strains in calves suggest that these strains could be a major health threat for humans in the future. In addition, the poor association between results of the geno- and phenotypical tests to screen for the AE ability of STEC strains calls the diagnostic value of the FAS test into question.

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Shiga toxin-producing Escherichia coli in healthy young beef steers from Argentina: prevalence and virulence properties

International Journal of Food Microbiology ◽

10.1016/j.ijfoodmicro.2004.03.018 ◽

2004 ◽

Vol 96 (2) ◽

pp. 189-198 ◽

Cited By ~ 63

Author(s):

Lelis Meichtri ◽

Elizabeth Miliwebsky ◽

Andrea Gioffré ◽

Isabel Chinen ◽

Ariela Baschkier ◽

...

Keyword(s):

Escherichia Coli ◽

Shiga Toxin ◽

Beef Steers ◽

Virulence Properties

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Dynamics of total and Shiga-toxin (Stx) forming Escherichia coli shedding with faeces of dairy cows experiencing a change from a low to a high caloric diet

Journal of Consumer Protection and Food Safety ◽

10.1007/s00003-014-0917-5 ◽

2014 ◽

Vol 10 (1) ◽

pp. 23-28

Author(s):

S. Dänicke ◽

U. Meyer ◽

L. Geue

Keyword(s):

Escherichia Coli ◽

Dairy Cows ◽

Shiga Toxin ◽

High Caloric Diet

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Shiga toxin-producing Escherichia coli and enteropathogenic Escherichia coli in healthy goats in India: occurrence and virulence properties

Journal of Applied Microbiology ◽

10.1111/j.1365-2672.2005.02759.x ◽

2006 ◽

Vol 100 (1) ◽

pp. 108-113 ◽

Cited By ~ 14

Author(s):

S.A. Wani ◽

I. Samanta ◽

Z.H. Munshi ◽

M.A. Bhat ◽

Y. Nishikawa

Keyword(s):

Escherichia Coli ◽

Shiga Toxin ◽

Enteropathogenic Escherichia Coli ◽

Virulence Properties

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Public health aspects of Shiga toxin-producing Escherichia coli (STEC) strains in sheep and goats of Bakhtiari pastoral tribe, Iran

Tropical Animal Health and Production ◽

10.1007/s11250-020-02245-2 ◽

2020 ◽

Vol 52 (5) ◽

pp. 2721-2724

Author(s):

Hassan Zaheri ◽

Reza Ghanbarpour ◽

Maziar Jajarmi ◽

Mahboube Bagheri ◽

Ali Ghanadian ◽

...

Keyword(s):

Public Health ◽

Escherichia Coli ◽

Shiga Toxin ◽

Sheep And Goats

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Zoonotic Agents in Small Ruminants Kept on City Farms in Southern Germany

Applied and Environmental Microbiology ◽

10.1128/aem.07802-11 ◽

2012 ◽

Vol 78 (11) ◽

pp. 3785-3793 ◽

Cited By ~ 13

Author(s):

Anna-Katarina Schilling ◽

Helmut Hotzel ◽

Ulrich Methner ◽

Lisa D. Sprague ◽

Gernot Schmoock ◽

...

Keyword(s):

Escherichia Coli ◽

Coxiella Burnetii ◽

Shiga Toxin ◽

Small Ruminants ◽

Southern Germany ◽

Content Type ◽

Sheep And Goats ◽

Close Contacts

ABSTRACTSheep and goats are popular examples of livestock kept on city farms. In these settings, close contacts between humans and animals frequently occur. Although it is widely accepted that small ruminants can carry numerous zoonotic agents, it is unknown which of these agents actually occur in sheep and goats on city farms in Germany. We sampled feces and nasal liquid of 48 animals (28 goats, 20 sheep) distributed in 7 city farms and on one activity playground in southern Germany. We found that 100% of the sampled sheep and 89.3% of the goats carried Shiga toxin-producingEscherichia coli(STEC). The presence ofStaphylococcusspp. in 75% of both sheep and goats could be demonstrated.Campylobacterspp. were detected in 25% and 14.3% of the sheep and goats, respectively. NeitherSalmonellaspp. norCoxiella burnetiiwas found. On the basis of these data, we propose a reasonable hygiene scheme to prevent transmission of zoonotic agents during city farm visits.

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