scholarly journals Comparison of PCR, BIO-PCR, DIA, ELISA and isolation on semiselective medium for detection of Xanthomonas albilineans, the causal agent of leaf scald of sugarcane

1999 ◽  
Vol 48 (2) ◽  
pp. 245-252 ◽  
Author(s):  
Wang ◽  
Comstock ◽  
Hatziloukas ◽  
Schaad
Keyword(s):  
Causal Agent ◽  
Leaf Scald ◽  
Xanthomonas Albilineans

Molecular detection and prevalence of Xanthomonas albilineans, the causal agent of sugarcane leaf scald, in China

2018 ◽  
Vol 109 ◽  
pp. 17-23 ◽  
Author(s):  
Ling-Hong Lin ◽  
Mbuya Sylvain Ntambo ◽  
Philippe C. Rott ◽  
Qin-Nan Wang ◽  
Yi-Hua Lin ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Causal Agent ◽  
Leaf Scald ◽  
Xanthomonas Albilineans

scholarly journals A Polymerase Chain Reaction Protocol for the Detection of Xanthomonas albilineans, the Causal Agent of Sugarcane Leaf Scald Disease

Plant Disease ◽  
1997 ◽  
Vol 81 (2) ◽  
pp. 189-194 ◽  
Author(s):  
Y.-B. Pan ◽  
M. P. Grisham ◽  
D. M. Burner
Keyword(s):  
Polymerase Chain Reaction ◽  
Blot Hybridization ◽  
Causal Agent ◽  
Rrna Genes ◽  
23S Rrna ◽  
Dot Blot ◽  
Chain Reaction ◽  
Leaf Scald ◽  
Polymerase Chain ◽  
Xanthomonas Albilineans

A polymerase chain reaction (PCR) protocol was developed that amplified a 360-bp DNA product unique to Xanthomonas albilineans (Xa), the causal agent of sugarcane leaf scald disease. The assay utilizes previously described PCR primers that target the intergenic transcribed spacer (ITS) region between the 16S and 23S rRNA genes. Primer pair Ala4/L1 allowed amplification of a 360-bp DNA fragment from 71 Xa strains including representatives of serovars I, II, and III. Fragments of different sizes were also amplified from three unidentified saprophytic bacteria from sugarcane. Xa could be detected at a lower bacterial concentration with the PCR protocol than with a serological dot blot assay. With PCR, as little as 1.25 pg of Xa genomic DNA (125 fg if followed by Southern blot hybridization), or as few as 0 to 5 CFU of Xa per reaction were detected from infected sugarcane sap and leaf diffusate. Five CFU of Xa per reaction were detected from suspension culture. The PCR protocol provides a rapid, reliable, and economical tool for routine detection and identification of Xa.

scholarly journals Pathogenic Variation in Xanthomonas Albilineans (Ashby) Dowson, the Causal Agent of Leaf-Scald Disease of Sugar Cane

1973 ◽  
Vol 26 (4) ◽  
pp. 781 ◽  
Author(s):  
GJ Persley
Keyword(s):  
Sugar Cane ◽  
Causal Agent ◽  
Leaf Scald ◽  
Pathogenic Variation ◽  
Xanthomonas Albilineans

The comparative pathogenicity of four isolates of X. albilineans was examined on 13 varieties of sugar cane. The isolates could be differentiated on the basis of their aggressiveness on seven varieties. The significance of varying aggressiveness of isolates in the epiphytology of the disease, and in the screening of varieties, is discussed.

scholarly journals Identification of Differentially Expressed Proteins in Sugarcane in Response to Infection by Xanthomonas albilineans Using iTRAQ Quantitative Proteomics

2020 ◽  
Vol 8 (1) ◽  
pp. 76
Author(s):  
Jian-Yu Meng ◽  
Mbuya Sylvain Ntambo ◽  
Philippe C. Rott ◽  
Hua-Ying Fu ◽  
Mei-Ting Huang ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Vascular System ◽  
Lipid Transfer Protein ◽  
Differentially Expressed ◽  
Post Inoculation ◽  
Differentially Expressed Proteins ◽  
Lipid Transfer ◽  
Leaf Scald ◽  
Improvement Programs ◽  
Xanthomonas Albilineans

Sugarcane can suffer severe yield losses when affected by leaf scald, a disease caused by Xanthomonas albilineans. This bacterial pathogen colonizes the vascular system of sugarcane, which can result in reduced plant growth and plant death. In order to better understand the molecular mechanisms involved in the resistance of sugarcane to leaf scald, a comparative proteomic study was performed with two sugarcane cultivars inoculated with X. albilineans: one resistant (LCP 85-384) and one susceptible (ROC20) to leaf scald. The iTRAQ (isobaric tags for relative and absolute quantification) approach at 0 and 48 h post-inoculation (hpi) was used to identify and annotate differentially expressed proteins (DEPs). A total of 4295 proteins were associated with 1099 gene ontology (GO) terms by GO analysis. Among those, 285 were DEPs during X. albilineans infection in cultivars LCP 85-384 and ROC20. One hundred seventy-two DEPs were identified in resistant cultivar LCP 85-384, and 113 of these proteins were upregulated and 59 were downregulated. One hundred ninety-two DEPs were found in susceptible cultivar ROC20 and half of these (92) were upregulated, whereas the other half corresponded to downregulated proteins. The significantly upregulated DEPs in LCP 85-384 were involved in metabolic pathways, the biosynthesis of secondary metabolites, and the phenylpropanoid biosynthesis pathway. Additionally, the expression of seven candidate genes related to photosynthesis and glycolytic pathways, plant innate immune system, glycosylation process, plant cytochrome P450, and non-specific lipid transfer protein was verified based on transcription levels in sugarcane during infection by X. albilineans. Our findings shed new light on the differential expression of proteins in sugarcane cultivars in response to infection by X. albilineans. The identification of these genes provides important information for sugarcane variety improvement programs using molecular breeding strategies.

scholarly journals PENGARUH METODE STERILISASI DAN KONSENTRASI FILTRAT BAKTERI ENDOFIT TANAMAN TEBU UNTUK MENGENDALIKAN PENYAKIT BLENDOK (Xanthomonas albilineans (Ashby) Savulescu 1947)

2017 ◽  
Vol 4 (1) ◽  
pp. 20-39
Author(s):  
Astri Afriani
Keyword(s):  
Endophytic Bacteria ◽  
Membrane Filter ◽  
Petri Dish ◽  
Hot Water ◽  
Control Measures ◽  
Leaf Scald ◽  
Randomized Design ◽  
Cutting Knife ◽  
North Sumatra ◽  
Xanthomonas Albilineans

Leaf scald (Xanthomonas albilineans) is an important pathogen of sugar cane in Indonesiaand cause significant losses at several main sugarcane plantations. Some control measures, e.i.bactericides smeared Lysol 20% or alcohol 70% on cutting knife seedling for disinfectant,and hot water treatment at 50 minutes on 52,5ᵒC, have not given satisfactory results inmanaging this leaf scald in sugarcane on the field. Biological control with filtrate ofendophytes bacterial were alternative to biocontrol agents Xanthomonas albilineans. Theobjectives of this research were to determine: influence of sterilization methods andendophytic bacterial filtrate dosages of sugarcane to control leaf scald (Xanthomonasalbilineans). This research was conducted in Phytopathology Laboratory at the Faculty ofAgriculture University of North Sumatra from April until December 2014. This research haddone with Completely Randomized Design (CRD) with three-factor: 1. type of endophyticbacterial filtrates: filtrate of B2, B7 and B9 Sterilization methods of endophytic bacterialfiltrate: control (no sterilization), Sterilization by autoclave and Sterilization by filtermembrane, 3. Dosage of endophytic filtrate with four-level: 0,1%, 0,01%, 0,001% and0,0001%. The result showed: 10 isolate endophytic bacteria had exploration were: B1, B2,B3, B4, B5, B6, B7, B8, B9, and B10. Three isolates had a clear zone on Petri dish ≥ 20 mm invitro testing is B9, B7, and B2. The results showed that the membrane filter sterilizationmethod was better when compared to autoclave sterilization, namely: the highest inhibitoryzone diameter of endophytic bacteria in the treatment of B9 filtrate with a membrane filtersterilization method 0,1% at 3 days after inoculation that was equal to 23.00 mm.

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