Detection of the Sugarcane Leaf Scald Pathogen,Xanthomonas albilineans,Using Tissue Blot Immunoassay, ELISA, and Isolation Techniques

Plant Disease ◽  
1992 ◽  
Vol 76 (10) ◽  
pp. 1033 ◽  
Author(s):  
J. C. Comstock
Keyword(s):  
Leaf Scald ◽  
Isolation Techniques ◽  
Tissue Blot Immunoassay ◽  
Xanthomonas Albilineans

scholarly journals Identification of Differentially Expressed Proteins in Sugarcane in Response to Infection by Xanthomonas albilineans Using iTRAQ Quantitative Proteomics

2020 ◽  
Vol 8 (1) ◽  
pp. 76
Author(s):  
Jian-Yu Meng ◽  
Mbuya Sylvain Ntambo ◽  
Philippe C. Rott ◽  
Hua-Ying Fu ◽  
Mei-Ting Huang ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Vascular System ◽  
Lipid Transfer Protein ◽  
Differentially Expressed ◽  
Post Inoculation ◽  
Differentially Expressed Proteins ◽  
Lipid Transfer ◽  
Leaf Scald ◽  
Improvement Programs ◽  
Xanthomonas Albilineans

Sugarcane can suffer severe yield losses when affected by leaf scald, a disease caused by Xanthomonas albilineans. This bacterial pathogen colonizes the vascular system of sugarcane, which can result in reduced plant growth and plant death. In order to better understand the molecular mechanisms involved in the resistance of sugarcane to leaf scald, a comparative proteomic study was performed with two sugarcane cultivars inoculated with X. albilineans: one resistant (LCP 85-384) and one susceptible (ROC20) to leaf scald. The iTRAQ (isobaric tags for relative and absolute quantification) approach at 0 and 48 h post-inoculation (hpi) was used to identify and annotate differentially expressed proteins (DEPs). A total of 4295 proteins were associated with 1099 gene ontology (GO) terms by GO analysis. Among those, 285 were DEPs during X. albilineans infection in cultivars LCP 85-384 and ROC20. One hundred seventy-two DEPs were identified in resistant cultivar LCP 85-384, and 113 of these proteins were upregulated and 59 were downregulated. One hundred ninety-two DEPs were found in susceptible cultivar ROC20 and half of these (92) were upregulated, whereas the other half corresponded to downregulated proteins. The significantly upregulated DEPs in LCP 85-384 were involved in metabolic pathways, the biosynthesis of secondary metabolites, and the phenylpropanoid biosynthesis pathway. Additionally, the expression of seven candidate genes related to photosynthesis and glycolytic pathways, plant innate immune system, glycosylation process, plant cytochrome P450, and non-specific lipid transfer protein was verified based on transcription levels in sugarcane during infection by X. albilineans. Our findings shed new light on the differential expression of proteins in sugarcane cultivars in response to infection by X. albilineans. The identification of these genes provides important information for sugarcane variety improvement programs using molecular breeding strategies.

scholarly journals PENGARUH METODE STERILISASI DAN KONSENTRASI FILTRAT BAKTERI ENDOFIT TANAMAN TEBU UNTUK MENGENDALIKAN PENYAKIT BLENDOK (Xanthomonas albilineans (Ashby) Savulescu 1947)

2017 ◽  
Vol 4 (1) ◽  
pp. 20-39
Author(s):  
Astri Afriani
Keyword(s):  
Endophytic Bacteria ◽  
Membrane Filter ◽  
Petri Dish ◽  
Hot Water ◽  
Control Measures ◽  
Leaf Scald ◽  
Randomized Design ◽  
Cutting Knife ◽  
North Sumatra ◽  
Xanthomonas Albilineans

Leaf scald (Xanthomonas albilineans) is an important pathogen of sugar cane in Indonesiaand cause significant losses at several main sugarcane plantations. Some control measures, e.i.bactericides smeared Lysol 20% or alcohol 70% on cutting knife seedling for disinfectant,and hot water treatment at 50 minutes on 52,5ᵒC, have not given satisfactory results inmanaging this leaf scald in sugarcane on the field. Biological control with filtrate ofendophytes bacterial were alternative to biocontrol agents Xanthomonas albilineans. Theobjectives of this research were to determine: influence of sterilization methods andendophytic bacterial filtrate dosages of sugarcane to control leaf scald (Xanthomonasalbilineans). This research was conducted in Phytopathology Laboratory at the Faculty ofAgriculture University of North Sumatra from April until December 2014. This research haddone with Completely Randomized Design (CRD) with three-factor: 1. type of endophyticbacterial filtrates: filtrate of B2, B7 and B9 Sterilization methods of endophytic bacterialfiltrate: control (no sterilization), Sterilization by autoclave and Sterilization by filtermembrane, 3. Dosage of endophytic filtrate with four-level: 0,1%, 0,01%, 0,001% and0,0001%. The result showed: 10 isolate endophytic bacteria had exploration were: B1, B2,B3, B4, B5, B6, B7, B8, B9, and B10. Three isolates had a clear zone on Petri dish ≥ 20 mm invitro testing is B9, B7, and B2. The results showed that the membrane filter sterilizationmethod was better when compared to autoclave sterilization, namely: the highest inhibitoryzone diameter of endophytic bacteria in the treatment of B9 filtrate with a membrane filtersterilization method 0,1% at 3 days after inoculation that was equal to 23.00 mm.

Molecular detection and prevalence of Xanthomonas albilineans, the causal agent of sugarcane leaf scald, in China

2018 ◽  
Vol 109 ◽  
pp. 17-23 ◽  
Author(s):  
Ling-Hong Lin ◽  
Mbuya Sylvain Ntambo ◽  
Philippe C. Rott ◽  
Qin-Nan Wang ◽  
Yi-Hua Lin ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Causal Agent ◽  
Leaf Scald ◽  
Xanthomonas Albilineans

Molecular detection and quantification of Xanthomonas albilineans in juice from symptomless sugarcane stalks using a real-time quantitative PCR assay

Plant Disease ◽  
2021 ◽  
Author(s):  
Yang Shi ◽  
Jian-Ying Zhao ◽  
Jing-Ru Zhou ◽  
Mbuya Sylvain Ntambo ◽  
Peng-Yuan Xu ◽  
...  
Keyword(s):  
Qpcr Assay ◽  
Taqman Probe ◽  
Bacterial Suspension ◽  
Bacterial Disease ◽  
Conventional Pcr ◽  
Pcr Assay ◽  
Leaf Scald ◽  
Sugarcane Production ◽  
Xanthomonas Albilineans ◽  
Detection And Quantification

Leaf scald, a bacterial disease caused by Xanthomonas albilineans (Ashby) Dowson, is a major limiting factor for sugarcane production worldwide. Accurate identification and quantification of X. albilineans is a prerequisite for successful management of this disease. A very sensitive and robust qPCR assay was developed in this study for detection and quantification of X. albilineans using TaqMan probe and primers targeting a putative adenosine triphosphate-binding cassette (ABC) transporter gene (abc). The novel qPCR assay was highly specific to the 43 tested X. albilineans strains belonging to different pulsed-field gel electrophoresis (PFGE) groups. The detection thresholds were 100 copies/µL of plasmid DNA, 100 fg/µL of bacterial genomic DNA, and 100 CFU/ml of bacterial suspension prepared from pure culture. This qPCR assay was 100 times more sensitive than a conventional PCR assay. The pathogen was detected by qPCR in 75.1% (410/546) symptomless stalk samples, whereas only 28.4% (155/546) samples tested positive by conventional PCR. Based on qPCR data, population densities of X. albilineans in symptomless stalks of the same varieties differed between two sugarcane production areas in China, Beihai (Guangxi province) and Zhanjiang (Guangdong province), and no significant correlation between these populations was identified. Furthermore, no relationship was found between these populations of the pathogen in asymptomatic stalks and the resistance level of the sugarcane varieties to leaf scald. The newly developed qPCR assay proved to be highly sensitive and reliable for the detection and quantification of X. albilineans in sugarcane stalks.

scholarly journals First Report of Xanthomonas albilineans Causing Leaf Scald on Two Chewing Cane Clones in Zhejiang Province, China

Plant Disease ◽  
2020 ◽  
pp. PDIS-06-20-1312
Author(s):  
Yao-Yao Duan ◽  
Yu-Qing Zhang ◽  
Zhe-Xin Xu ◽  
Yi Lin ◽  
Ling-Rong Mao ◽  
...  
Keyword(s):  
Zhejiang Province ◽  
Leaf Scald ◽  
First Report ◽  
Xanthomonas Albilineans

scholarly journals Substrate Specificity-Conferring Regions of the Nonribosomal Peptide Synthetase Adenylation Domains Involved in Albicidin Pathotoxin Biosynthesis Are Highly Conserved within the Species Xanthomonas albilineans

2007 ◽  
Vol 73 (17) ◽  
pp. 5523-5530 ◽  
Author(s):  
Adeline Renier ◽  
Eric Vivien ◽  
Stéphane Cociancich ◽  
Philippe Letourmy ◽  
Xavier Perrier ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Amino Acid ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Leaf Scald ◽  
Peptide Synthetase ◽  
A Domains ◽  
Xanthomonas Albilineans

ABSTRACT Albicidin is a pathotoxin produced by Xanthomonas albilineans, a xylem-invading pathogen that causes leaf scald disease of sugarcane. Albicidin is synthesized by a nonribosomal pathway via modular polyketide synthase and nonribosomal peptide synthetase (NRPS) megasynthases, and NRPS adenylation (A) domains are responsible for the recognition and activation of specific amino acid substrates. DNA fragments (0.5 kb) encoding the regions responsible for the substrate specificities of six albicidin NRPS A domains from 16 strains of X. albilineans representing the known diversity of this pathogen were amplified and sequenced. Polymorphism analysis of these DNA fragments at different levels (DNA, protein, and NRPS signature) showed that these pathogenicity loci were highly conserved. The conservation of these loci most likely reflects purifying selective pressure, as revealed by a comparison with the variability of nucleotide and amino acid sequences of two housekeeping genes (atpD and efp) of X. albilineans. Nevertheless, the 16 strains of X. albilineans were differentiated into several groups by a phylogenetic analysis of the nucleotide sequences corresponding to the NRPS A domains. One of these groups was representative of the genetic diversity previously found within the pathogen by random fragment length polymorphism and amplified fragment length polymorphism analyses. This group, which differed by three single synonymous nucleotide mutations, contained only four strains of X. albilineans that were all involved in outbreaks of sugarcane leaf scald. The amount of albicidin produced in vitro in agar and liquid media varied among the 16 strains of X. albilineans. However, no relationship among the amount of albicidin produced in vitro and the pathotypes and genetic diversity of the pathogen was found. The NRPS loci contributing to the synthesis of the primary structure of albicidin apparently are not involved in the observed pathogenicity differences among strains of X. albilineans.

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