Evaluation of variability in Striga aspera, Striga hermonthica and their hybrids using morphological characters and random amplified polymorphic DNA markers

Weed Research ◽  
2000 ◽  
Vol 40 (4) ◽  
pp. 375-386 ◽  
Author(s):  
Aigbokhan ◽  
Berner ◽  
Musselman ◽  
Mignouna
Keyword(s):  
Dna Markers ◽  
Random Amplified Polymorphic Dna ◽  
Morphological Characters ◽  
Striga Hermonthica

scholarly journals IDENTIFIKASI VARIETAS KENTANG “SUPERJOHN” BERDASARKAN PENANDA RAPD (Random Amplified Polymorphic DNA)

EUGENIA ◽  
2011 ◽  
Vol 17 (1) ◽  
Author(s):  
David S. Runtunuwu ◽  
J. E.X. Rogi ◽  
J. H. Palendeng
Keyword(s):  
Molecular Markers ◽  
Molecular Marker ◽  
Potato Variety ◽  
Dna Markers ◽  
Random Amplified Polymorphic Dna ◽  
Field Research ◽  
Morphological Characters ◽  
North Sulawesi ◽  
Field Identification ◽  
Young Leaves

ABSTRACT Identification using morphological characters has time consuming. Currently, identification using molecular markers has now been popular due to rapid, saving time and more precisely. Superjohn potato variety has been cultivated in North Sulawesi. However, the Superjohn potato variety has not been characterized using molecular markers. This research was aiming to identify “Superjohn” potato based on RAPD (Random Amplified Polymorphic DNA) markers. The research was conducted in field and laboratory. Field research was performed by taking some young leaves from “Superjohn”, Granola, and Atlantic variety from the field. Identification using molecular marker was conducted at laboratory.  Nine RAPD primers were used to identify the superjohn variety. The nine primers were OPA-1, OPA-2, OPA-3, OPA-4, OPA-5, OPA-7, OPA-9,  OPA-10, and  OPO-1.  The molecular identification revealed that “Superjohn” variety was different with Granola and Atlantic. OPA-9700 primer could be used for identification  “Superjohn” variety while OPA-101000 primer was suitable  for identification  Granola variety. Keywords:  Potatoes, variety, “Superjohn”, Granola, Atlantic, and RAPD ABSTRAK Penelitian ini dilakukan untuk mengidentikasi kentang “Superjohn” berdasarkan penanda RAPD (Random Amplified Polymorphic DNA).  Penelitian dilakukan di lapangan dan laboratorium. Penelitian lapangan dilakukan dengan mengambil beberapa daun muda dari varietas kentang “Superjohn”, Atlantik dan Granola. Kemudian  analisis DNA dilakukan di laboratorium menggunakan analisis RAPD. Berdasarkan penanda RAPD ternyata kentang “Superjohn” berbeda dari kentang Granola dan kentang Atlantik. Penanda RAPD OPA-9700 dapat digunakan untuk mengidentifikasi kentang“Superjohn” dan penanda RAPD  OPA-101000 dapat digunakan  untuk mengidentifikasi kentang Granola. Kata kunci:  Kentang,  varietas, Superjohn, Granola, Granola, RAPD

scholarly journals IDENTIFIKASI VARIETAS KENTANG SUPERJOHN BERDASARKAN PENANDA RAPD (Random Amplified Polymorphic DNA)

EUGENIA ◽  
2011 ◽  
Vol 17 (1) ◽  
pp. 52
Author(s):  
Runtunuwu D. S. ◽  
J. E. X. Rogi ◽  
J. H. Palendeng
Keyword(s):  
Molecular Markers ◽  
Molecular Marker ◽  
Potato Variety ◽  
Dna Markers ◽  
Random Amplified Polymorphic Dna ◽  
Field Research ◽  
Morphological Characters ◽  
North Sulawesi ◽  
Field Identification ◽  
Young Leaves

Identification using morphological characters has time consuming. Currently, identification usingmolecular markers has now been popular due to rapid, saving time and more precisely. Superjohnpotato variety has been cultivated in North Sulawesi. However, the Superjohn potato variety has notbeen characterized using molecular markers. This research was aiming to identify ―Superjohn‖ potatobased on RAPD (Random Amplified Polymorphic DNA) markers. The research was conducted in fieldand laboratory. Field research was performed by taking some young leaves from ―Superjohn‖, Granola,and Atlantic variety from the field. Identification using molecular marker was conducted at laboratory.Nine RAPD primers were used to identify the superjohn variety. The nine primers were OPA-1, OPA-2,OPA-3, OPA-4, OPA-5, OPA-7, OPA-9, OPA-10, and OPO-1. The molecular identification revealedthat ―Superjohn‖ variety was different with Granola and Atlantic. OPA-9700 primer could be used foridentification ―Superjohn‖ variety while OPA-101000 primer was suitable for identification Granolavariety.Keywords: Potatoes, variety, “Superjohn”, Granola, Atlantic, and RAPD ABSTRAKPenelitian ini dilakukan untuk mengidentikasi kentang ―Superjohn‖ berdasarkan penanda RAPD(Random Amplified Polymorphic DNA). Penelitian dilakukan di lapangan dan laboratorium. Penelitianlapangan dilakukan dengan mengambil beberapa daun muda dari varietas kentang ―Superjohn‖,Atlantik dan Granola. Kemudian analisis DNA dilakukan di laboratorium menggunakan analisis RAPD.Berdasarkan penanda RAPD ternyata kentang ―Superjohn‖ berbeda dari kentang Granola dan kentangAtlantik. Penanda RAPD OPA-9700 dapat digunakan untuk mengidentifikasi kentang―Superjohn‖ danpenanda RAPD OPA-101000 dapat digunakan untuk mengidentifikasi kentang Granola.

scholarly journals Assessment of genetic diversity among surian Toona sinensis Roem in progenies test using random amplified polymorphic DNA markers

2018 ◽  
Vol 22 (1) ◽  
pp. 22
Author(s):  
Jayusman Jayusman ◽  
Muhammad Na’iem ◽  
Sapto Indrioko ◽  
Eko Bhakti Hardiyanto ◽  
ILG Nurcahyaningsih
Keyword(s):  
Genetic Diversity ◽  
Dna Markers ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Management Strategies ◽  
Genetic Distances ◽  
Sampled Data ◽  
Toona Sinensis ◽  
The Mean ◽  
Individual Trees

Surian Toona sinensis Roem is one of the most widely planted species in Indonesia. This study aimed to estimate the genetic diversity between a number of surian populations in a progeny test using RAPD markers, with the goal of proposing management strategies for a surian breeding program. Ninety-six individual trees from 8 populations of surian were chosen as samples for analysis. Eleven polymorphic primers (OP-B3, OP-B4, OP-B10, OP-H3, OP-Y6, OP-Y7, OP-Y8, OP-Y10, OP-Y11, OP-Y14, and OP-06) producing reproducible bands were analyzed for the 96 trees, with six trees per family sampled. Data were analyzed using GenAlEx 6.3, NTSYS 2.02. The observed percentage of polymorphic loci ranged from 18.2% to 50%. The mean level of genetic diversity among the surian populations was considered to be moderate (He 0.304). Cluster analysis grouped the genotypes into two main clusters, at similarity levels of 0.68 and 0.46. The first two axes of the PCoA explained 46.16% and 25.54% of the total variation, respectively. The grouping of samples into clusters and subclusters did not correspond with family and their distances, but the grouping was in line with the genetic distances of the samples.

scholarly journals The Genetic Variability of Sicilian Lemon Germplasm Revealed by Molecular Marker Fingerprints

2008 ◽  
Vol 133 (2) ◽  
pp. 242-248 ◽  
Author(s):  
Mirko Siragusa ◽  
Fabio De Pasquale ◽  
Loredana Abbate ◽  
Letizia Martorana ◽  
Nicasio Tusa
Keyword(s):  
Dna Markers ◽  
Random Amplified Polymorphic Dna ◽  
Citrus Limon ◽  
Genetic Isolation ◽  
Breeding Programs ◽  
Bud Mutation ◽  
Italian Regions ◽  
Wide Range ◽  
High Level ◽  
Polymorphic Nature

There is a high level of diversity among lemons [Citrus limon (L.) Burm. f. (2n = 2x = 18)] in Sicily, where each growing area has a wide range of landraces mostly derived from bud mutation. Because this variability represents an important resource for future breeding programs and genetic improvement, the relationships among the principal 36 accessions of Sicilian lemon, belonging to three different cultivars (Femminello, Monachello, and Lunario), were examined by intersimple sequence repeat and random amplified polymorphic DNA markers. Three ‘Femminello’ accessions from nearby Italian regions were also examined to study the genetic flow from the continent. The disputed case of the accession ‘Eureka Messina lemon’ was also examined, using ‘Frost Eureka’ as a control. Our results confirmed the extreme polymorphic nature of the three principal Sicilian cultivars and the presence of a wide range of different genotypes. Twenty-two Sicilian genotypes were recognized as unique accessions, reflecting the richness of the lemon germplasm present in Sicily. Each growing area showed the presence of several genetically different landraces, probably preserved by genetic isolation, whereas the continental accessions appeared extremely similar to the island genotypes, showing an exchange of germplasm from the island to the continent.

Segregation of random amplified polymorphic DNA markers and strategies for molecular mapping in tetraploid alfalfa

Genome ◽  
1993 ◽  
Vol 36 (5) ◽  
pp. 844-851 ◽  
Author(s):  
K. F. Yu ◽  
K. P. Pauls
Keyword(s):  
Chromosome Segregation ◽  
Dna Markers ◽  
Rapd Markers ◽  
Molecular Mapping ◽  
Random Amplified Polymorphic Dna ◽  
Rapd Marker ◽  
Female Parent ◽  
Linkage Groups ◽  
Double Reduction ◽  
Chromatid Segregation

An F1 population was used to analyze the inheritance of random amplified polymorphic DNA (RAPD) markers in tetraploid alfalfa. Of the 32 RAPD markers that were used for a segregation analysis in this study, 27 gave ratios that are consistent with random chromosome and random chromatid segregation at meiosis. However, among all of the RAPD markers (121) that were screened in this study, only one example of a double reduction, that is typical of chromatid segregation, was observed. These results indicate that random chromosome segregation is likely the predominant but not the exclusive mode of inheritance for tetraploid alfalfa. χ2 analyses of cosegregation for RAPD marker pairs derived from the female parent revealed nine linkages that fell into four linkage groups. The recombination fractions among linked marker pairs ranged from 1 to 37%. These are the first molecular linkage groups reported in tetraploid alfalfa. In addition, various strategies for molecular mapping in the tetraploid alfalfa genome are proposed that should be of interest to plant breeders who are planning to use molecular markers for alfalfa or other tetraploid species.Key words: RAPD markers, tetraploid alfalfa, segregation, linkage groups.

Genetic mapping and non-Mendelian segregation of mating type loci in the oomycete, Phytophthora infestans.

Genetics ◽  
1995 ◽  
Vol 141 (2) ◽  
pp. 503-512 ◽  
Author(s):  
H S Judelson ◽  
L J Spielman ◽  
R C Shattock
Keyword(s):  
Phytophthora Infestans ◽  
Mating Type ◽  
Dna Markers ◽  
Bulked Segregant Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Mating Types ◽  
Mendelian Segregation ◽  
Range Restriction ◽  
Type Locus ◽  
Mating Type Locus

Abstract DNA markers linked to the determinants of mating type in the oomycete, Phytophthora infestans, were identified and used to address the genetic basis of heterothallism in the normally diploid fungus. Thirteen loci linked to the A1 and A2 mating types were initially identified by bulked segregant analysis using random amplified polymorphic DNA markers (RAPDs) and subsequently scored in three crosses polymorphisms (SSCP), cleaved amplified polymorphisms (CAPS), or allele-specific polymerase chain reaction markers (AS-PCR). All DNA markers mapped to a single region, consistent with a single locus determining both mating types. Long-range restriction mapping also demonstrated the linkage of the markers to one region and delimited the mating type locus to a 100-kb region. The interval containing the mating type locus displayed non-Mendelian segregation as only two of the four expected genotypes were detected in progeny. This is consistent with a system of balance lethal loci near the mating type locus. A model for mating type determination is presented in which the balanced lethals exclude form progeny those with potentially conflicting combinations of mating type alleles, such as those simultaneously expressing A1 and A2 functions.

Sign in / Sign up

Export Citation Format

Share Document