Neutral N-glycans in adult rat brain tissue. Complete characterisation reveals fucosylated hybrid and complex structures

1998 ◽  
Vol 251 (3) ◽  
pp. 691-703 ◽  
Author(s):  
Yi-Ju Chen ◽  
David R. Wing ◽  
Geoffrey R. Guile ◽  
Raymond A. Dwek ◽  
David J. Harvey ◽  
...  
Keyword(s):  
Rat Brain ◽  
Brain Tissue ◽  
Complex Structures ◽  
Adult Rat ◽  
Adult Rat Brain ◽  
Rat Brain Tissue ◽  
Complete Characterisation

scholarly journals Oxidative stress induced by the Fe2+/ascorbic acid system or model ischemia in vitro: effect of carvedilol and pyridoindole antioxidant SMe1EC2 in young and adult rat brain tissue

2010 ◽  
Vol 3 (4) ◽  
pp. 122-126 ◽  
Author(s):  
Zdenka Gáspárová ◽  
Oľga Ondrejičková ◽  
Alena Gajdošíková ◽  
Andrej Gajdošík ◽  
Vladimír Šnirc ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Ascorbic Acid ◽  
Rat Brain ◽  
Brain Tissue ◽  
Protein Carbonyl ◽  
Experimental Models ◽  
Adult Rat ◽  
Adult Rat Brain ◽  
Rat Brain Tissue

Oxidative stress induced by the Fe2+/ascorbic acid system or model ischemiain vitro: effect of carvedilol and pyridoindole antioxidant SMe1EC2 in young and adult rat brain tissueNew effective strategies and new highly effective neuroprotective agents are being searched for the therapy of human stroke and cerebral ischemia. The compound SMe1EC2 is a new derivative of stobadine, with enhanced antioxidant properties compared to the maternal drug. Carvedilol, a non-selective beta-blocker, possesses besides its cardioprotective and vasculoprotective properties also an antioxidant effect. We compared the effect of carvedilol and SMe1EC2, antioxidants with a similar chemical structure, in two experimental models of oxidative stress in young and adult rat brain tissue. SMe1EC2 was found to improve the resistance of hippocampal neurons to ischemiain vitroin young and even in 18-month-old rats and inhibited formation of protein carbonyl groups induced by the Fe2+/ascorbic acid pro-oxidative system in brain cortex homogenates of young rats. Carvedilol exerted a protective effect only in the hippocampus of 2-month-old rats and that at the concentration 10-times higher than did SMe1EC2. The inhibitory effect of carvedilol on protein carbonyl formation induced by the pro-oxidative system was not proved in the cortex of either young or adult rats. An increased baseline level of the content of protein carbonyl groups in the adult versus young rat brain cortex confirmed age-related changes in neuronal tissue and may be due to increased production of reactive oxygen species and low antioxidant defense mechanisms in the adult rat brain. The results revealed the new pyridoindole SMe1EC2 to be more effective than carvedilol in neuroprotection of rat brain tissue in both experimental models involving oxidative stress.

Chronic response of adult rat brain tissue to implants anchored to the skull

Biomaterials ◽  
2004 ◽  
Vol 25 (12) ◽  
pp. 2229-2237 ◽  
Author(s):  
Young-Tae Kim ◽  
Robert W Hitchcock ◽  
Michael J Bridge ◽  
Patrick A Tresco
Keyword(s):  
Rat Brain ◽  
Brain Tissue ◽  
Adult Rat ◽  
Adult Rat Brain ◽  
Rat Brain Tissue

Sialylated N-glycans in adult rat brain tissue. A widespread distribution of disialylated antennae in complex and hybrid structures

1998 ◽  
Vol 258 (1) ◽  
pp. 243-270 ◽  
Author(s):  
Susanne Zamze ◽  
David J. Harvey ◽  
Yi-Ju Chen ◽  
Geoffrey R. Guile ◽  
Raymond A. Dwek ◽  
...  
Keyword(s):  
Rat Brain ◽  
Brain Tissue ◽  
Hybrid Structures ◽  
Widespread Distribution ◽  
Adult Rat ◽  
Adult Rat Brain ◽  
Rat Brain Tissue

Expression of neurocan after transient middle cerebral artery occlusion in adult rat brain

2005 ◽  
Vol 25 (1_suppl) ◽  
pp. S217-S217
Author(s):  
Kentaro Deguchi ◽  
Mikiro Takaishi ◽  
Takeshi Hayashi ◽  
Atsuhiko Oohira ◽  
Shoko Nagotani ◽  
...  
Keyword(s):  
Middle Cerebral Artery ◽  
Rat Brain ◽  
Middle Cerebral Artery Occlusion ◽  
Cerebral Artery ◽  
Artery Occlusion ◽  
Adult Rat ◽  
Adult Rat Brain ◽  
Cerebral Artery Occlusion

Liquid Chromatography Analysis of Clozapine in Rat Brain Tissue, Using its Molecularly Imprinted Polymer after Administration of Toxic Dose of Drug and Lipid Emulsion Therapy

2019 ◽  
Vol 15 (3) ◽  
pp. 251-257
Author(s):  
Bahareh Sadat Yousefsani ◽  
Seyed Ahmad Mohajeri ◽  
Mohammad Moshiri ◽  
Hossein Hosseinzadeh
Keyword(s):  
Rat Brain ◽  
Brain Tissue ◽  
Molecularly Imprinted Polymer ◽  
Lipid Emulsion ◽  
Limit Of Detection ◽  
Imprinted Polymer ◽  
Toxic Dose ◽  
Molecularly Imprinted ◽  
The Brain ◽  
Rat Brain Tissue

Background:Molecularly imprinted polymers (MIPs) are synthetic polymers that have a selective site for a given analyte, or a group of structurally related compounds, that make them ideal polymers to be used in separation processes.Objective:An optimized molecularly imprinted polymer was selected and applied for selective extraction and analysis of clozapine in rat brain tissue.Methods:A molecularly imprinted solid-phase extraction (MISPE) method was developed for preconcentration and cleanup of clozapine in rat brain samples before HPLC-UV analysis. The extraction and analytical process was calibrated in the range of 0.025-100 ppm. Clozapine recovery in this MISPE process was calculated between 99.40 and 102.96%. The limit of detection (LOD) and the limit of quantification (LOQ) of the assay were 0.003 and 0.025 ppm, respectively. Intra-day precision values for clozapine concentrations of 0.125 and 0.025 ppm were 5.30 and 3.55%, whereas inter-day precision values of these concentrations were 9.23 and 6.15%, respectively. In this study, the effect of lipid emulsion infusion in reducing the brain concentration of drug was also evaluated.Results:The data indicated that calibrated method was successfully applied for the analysis of clozapine in the real rat brain samples after administration of a toxic dose to animal. Finally, the efficacy of lipid emulsion therapy in reducing the brain tissue concentration of clozapine after toxic administration of drug was determined.Conclusion:The proposed MISPE method could be applied in the extraction and preconcentration before HPLC-UV analysis of clozapine in rat brain tissue.

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