Non-Invasive Single Cell pH Measurements In The Isolated Perfused Pancreas

Natural Killer (NK) are indicated as favorite candidates for innovative therapeutic treatment and are divided in two subclasses: immature regulatory NK CD56bright and mature cytotoxic NK CD56dim. Therefore, the ability...

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Intracellular pH measurements in Kidney in vitro — New advantages on single cell diagnosis in the intact organ

Gastroenterology ◽

10.1016/s0016-5085(00)80241-3 ◽

2000 ◽

Vol 118 (4) ◽

pp. A1109

Author(s):

Michael J. Sessler ◽

Christina Baumstark ◽

Joerg Weik ◽

Michael Weinlich ◽

Richard Viebahn ◽

...

Keyword(s):

Single Cell ◽

Intracellular Ph ◽

Ph Measurements ◽

Single Cell Diagnosis

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Effect of galanin on glucose-, arginine-, or potassium-stimulated insulin release

AJP Endocrinology and Metabolism ◽

10.1152/ajpendo.1989.256.5.e619 ◽

1989 ◽

Vol 256 (5) ◽

pp. E619-E623

Author(s):

T. Yoshimura ◽

J. Ishizuka ◽

G. H. Greeley ◽

J. C. Thompson

Keyword(s):

Insulin Release ◽

High Glucose ◽

Second Phase ◽

Dependent Manner ◽

Perfused Pancreas ◽

Isolated Perfused Pancreas ◽

Second Phases ◽

High Concentrations ◽

Dose Dependent ◽

Stimulation Of

We have examined the effect of galanin infusion on glucose-stimulated release of insulin from the isolated perfused pancreas of the rat to better characterize the effect of galanin on the first and second phases of insulin release. The effects of galanin on insulin release stimulated by L-arginine or high concentrations of potassium were also examined. When perfusion of galanin was started 4 min before the start of perfusion of high glucose (16.7 mM), galanin (10(-8)-10(-11) M) inhibited both the first and second phases of insulin release in a dose-dependent manner. When perfusion of galanin (10(-8) or 10(-9) M) was started simultaneously with high glucose (16.7 mM), only the second phase of insulin release was suppressed (P less than 0.05). Galanin (10(-9) M) failed to inhibit insulin release stimulated by L-arginine (10 and 5 mM) or potassium (25 and 20 mM). These findings suggest that the inhibitory action of galanin on glucose-stimulated insulin release is exerted on early intracellular events that occur during the stimulation of insulin release and that are common to both phases. Because galanin does not inhibit insulin release stimulated by L-arginine or potassium, galanin may inhibit glucose-stimulated closure of potassium channels.

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Single Cell RNA Sequencing of Human Milk-Derived Cells Reveals Sub-Populations of Mammary Epithelial Cells with Molecular Signatures of Progenitor and Mature States: a Novel, Non-invasive Framework for Investigating Human Lactation Physiology

Journal of Mammary Gland Biology and Neoplasia ◽

10.1007/s10911-020-09466-z ◽

2020 ◽

Author(s):

Jayne F. Martin Carli ◽

G. Devon Trahan ◽

Kenneth L. Jones ◽

Nicole Hirsch ◽

Kristy P. Rolloff ◽

...

Keyword(s):

Epithelial Cells ◽

Human Milk ◽

Single Cell ◽

Rna Sequencing ◽

Mammary Epithelial Cells ◽

Mammary Epithelial ◽

Molecular Signatures ◽

Human Lactation ◽

Non Invasive ◽

Single Cell Rna Sequencing

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Differential inhibitory effects of noradrenaline upon insulin secretion by the isolated perfused pancreas of the rat

Cellular and Molecular Life Sciences ◽

10.1007/bf01955397 ◽

1982 ◽

Vol 38 (9) ◽

pp. 1116-1118 ◽

Cited By ~ 2

Author(s):

R. J. Howland ◽

G. M. Baroody

Keyword(s):

Insulin Secretion ◽

Perfused Pancreas ◽

Inhibitory Effects ◽

Isolated Perfused Pancreas

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α- and β-Cell Function in Obese Zucker (fa/fa) Rats: A Study with the Isolated Perfused Pancreas

Clinical Science ◽

10.1042/cs0860311 ◽

1994 ◽

Vol 86 (3) ◽

pp. 311-316 ◽

Cited By ~ 7

Author(s):

Dr Hiroshi Hirose ◽

Hiroshi Maruyama ◽

Koichi Kido ◽

Katsuhiko Ito ◽

Kazunori Koyama ◽

...

Keyword(s):

Glucose Concentration ◽

Cell Function ◽

Glucagon Secretion ◽

Zucker Rats ◽

Perfused Pancreas ◽

Isolated Perfused Pancreas ◽

Insulin And Glucagon Secretion ◽

Β Cell Function ◽

Insulin Secretion Rate ◽

Insulin Secretory Response

1. The effects of various stimuli, including changes in glucose concentration, arginine, tyramine and noradrenaline, on insulin and glucagon secretion were investigated using isolated perfused pancreata of obese and lean male Zucker rats at 12 months of age. 2. In Zucker fatty rats, the insulin secretion rate was significantly (P < 0.01) higher than that of lean rats at all glucose concentrations tested (8.3, 16.7 and 1.4 mmol/l). However, the integrated insulin secretory response to raising the glucose concentration from 8.3 to 16.7 mmol/l was almost absent in these rats. The glucagon secretion rates were significantly lower at 8.3 and 1.4 mmol/l glucose (P < 0.001 for both), and in responses to 10 μg/ml tyramine and 0.1 μmol/l noradrenaline (P < 0.05 for both), in Zucker fatty rats. Integrated insulin and glucagon responses to 10 mmol/l arginine were identical in the two groups. 3. Histopathological and immunochemical studies revealed hyperplasia of β-cells and scattered α-cells in the enlarged islets of Zucker fatty rats. 4. These results suggest that, in Zucker fatty rats, the decreased glucagon secretion in the isolated perfused pancreas is attributable to changes in the environment of α-cells and/or the inhibitory effects of hypersecreted insulin.

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