Previous maternal experience potentiates the effect of parturition on oxytocin receptor mRNA expression in the paraventricular nucleus

1999 ◽  
Vol 11 (10) ◽  
pp. 3725-3737 ◽  
Author(s):  
K. D. Broad ◽  
F. Lévy ◽  
G. Evans ◽  
T. Kimura ◽  
E. B. Keverne ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Paraventricular Nucleus ◽  
Oxytocin Receptor ◽  
Maternal Experience ◽  
Receptor Mrna

Localization of oxytocin receptor mRNA in the ovine uterus during the oestrous cycle and early pregnancy

1994 ◽  
Vol 12 (1) ◽  
pp. 93-105 ◽  
Author(s):  
K R Stevenson ◽  
P R Riley ◽  
H J Stewart ◽  
A P F Flint ◽  
D C Wathes
Keyword(s):  
Mrna Expression ◽  
Optical Density ◽  
Receptor Gene ◽  
Oxytocin Receptor ◽  
Oestrous Cycle ◽  
Luminal Epithelium ◽  
Binding Studies ◽  
Receptor Mrna ◽  
Oxytocin Receptors ◽  
Ovine Uterus

ABSTRACT A synthetic 45-mer oligonucleotide corresponding to part of the ovine endometrial oxytocin receptor cDNA was hybridized to sections of ovine uterus collected from 40 ewes at different stages during the oestrous cycle, the first 3 weeks of pregnancy and seasonal anoestrus. The quantity of oxytocin receptor mRNA was measured as the optical density (OD) value on autoradiographs using image analysis. Message first appeared in the luminal epithelium on days 14–15 of the cycle, increasing to a peak OD of 0·48 at oestrus and decreasing again between days 2 and 5. Oxytocin receptor mRNA in the superficial glands, deep glands and caruncular stroma increased between day 15 and oestrus to peak OD values of 0·17, 0·11 and 0·11 respectively, declining again by day 2 and reaching basal values (OD<0·015) by day 5. Hybridization to the myometrium tended to rise from a mean OD value of 0·01 on days 2–15 to a peak of 0·03±0·01 (mean±s.e.m.) on days 0–1, but the change was not significant. In pregnant ewes there was no detectable oxytocin receptor mRNA on days 14–15 in any region, but hybridization to the luminal epithelium was present in two of three ewes on day 21. In anoestrous ewes oxytocin receptor mRNA concentrations in all areas of the endometrium were approximately half those measured at oestrus. Optical density readings for oxytocin receptor mRNA in the various uterine compartments were compared with measurements of oxytocin receptors in the same regions as assessed by binding studies using the 125I-labelled oxytocin antagonist d(CH2)5[Tyr(Me)2,Thr4,Tyr-NH29]-vasotocin (125I-labelled OTA). In the endometrium, receptor mRNA and 125I-labelled OTA binding patterns changed in parallel, and both sets of measurements were significantly correlated (P<0·01). In the myometrium, a significant increase in 125I-labelled OTA binding occurred at oestrus; this was not accompanied by a similar increase in oxytocin receptor mRNA hybridization. This study helps to confirm that the previously identified cDNA clone is derived from the ovine oxytocin receptor, as patterns of oxytocin receptor mRNA expression in the endometrium closely resembled those of oxytocin binding. Maximum expression and binding both occurred at oestrus, suggesting that regulation of the oxytocin receptor gene in the uterus occurs principally at the transcriptional, rather than at the translational, level. Failure to detect a significant increase in myometrial mRNA expression at oestrus may indicate that the endometrial and myometrial oxytocin receptors are of different isoforms.

β2-agonist treatment enhances uterine oxytocin receptor mRNA expression in pregnant rats

2004 ◽  
Vol 69 (1) ◽  
pp. 60-65 ◽  
Author(s):  
Anna Klukovits ◽  
Eszter Ducza ◽  
Imre Földesi ◽  
George Falkay
Keyword(s):  
Mrna Expression ◽  
Oxytocin Receptor ◽  
Pregnant Rats ◽  
Receptor Mrna ◽  
Β2 Agonist

Developmental changes in hypothalamic oxytocin and oxytocin receptor mRNA expression and their sensitivity to fasting in male and female rats

2015 ◽  
Vol 41 (1) ◽  
pp. 105-109 ◽  
Author(s):  
Toshiya Matsuzaki ◽  
Takeshi Iwasa ◽  
Munkhsaikhan Munkhzaya ◽  
Altankhuu Tungalagsuvd ◽  
Takako Kawami ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Oxytocin Receptor ◽  
Developmental Changes ◽  
Female Rats ◽  
Male And Female ◽  
Receptor Mrna ◽  
Male And Female Rats

Effects of progesterone on expression of messenger RNA encoding oxytocin-neurophysin, oxytocin receptor and prostaglandin G/H synthase-1 and -2 during the early oestrous cycle in the ovine corpus luteum

1999 ◽  
Vol 11 (8) ◽  
pp. 435 ◽  
Author(s):  
H. Y. Al-Matubsi ◽  
S. Frazer ◽  
R. J. Fairclough ◽  
G. Jenkin
Keyword(s):  
Mrna Expression ◽  
Corpus Luteum ◽  
Messenger Rna ◽  
Corn Oil ◽  
Cdna Probe ◽  
Oxytocin Receptor ◽  
Corpora Lutea ◽  
Receptor Mrna ◽  
Progesterone Treatment ◽  
To Receive

This study was conducted to determine whether early progesterone treatment plays a role in the regulation of messenger RNA (mRNA) expression for oxytocin–neurophysin, oxytocin receptor, prostaglandin G/H synthase (PGHS)-1 and PGHS-2 in the ovine corpus luteum. The expression of ovarian oxytocin, oxytocin receptor, PGHS-1 and PGHS-2 mRNA was investigated in control, progesterone- or RU486-treated ewes. Fifteen ewes were randomly assigned to three groups to receive intramuscular injections of progesterone (12.5 mg; n = 5), RU486, (2.5 mg kg–1 bodyweight; n = 4) or corn oil (1 mL; n =6) twice daily from Day 1 to Day 3 post oestrus. On the morning of Day 4 post oestrus, the corpora lutea were collected and analysed for oxytocin–neurophysin mRNA by Northern blot using a labelled cDNA probe, and for the expressions of the oxytocin receptor, PGHS-1 and PGHS-2 mRNA using the reverse transcription polymerase chain reaction. Administration of progesterone or suppression of progesterone activity with RU486 did not affect expression of oxytocin–neurophysin mRNA (P>0.05). Pretreatment of the ewes with progesterone resulted in the enhancement of luteal oxytocin receptor mRNA expression and suppression of PGHS-1 and PGHS-2 mRNA (P<0.001). These results indicate that early progesterone treatment does not control the expression of oxytocin–neurophysin mRNA in the ovine ovary but may be involved in the regulation of ovarian oxytocin receptor and PGHS expression. It is proposed, on the basis of these results, that progesterone may play a role in premature corpus luteum regression through an intra-ovarian mechanism involving the induction of ovarian oxytocin receptor mRNA expression.

Vasopressin and oxytocin receptor mRNA expression during rat telencephalon development

Neuropeptides ◽  
2000 ◽  
Vol 34 (3-4) ◽  
pp. 173-180 ◽  
Author(s):  
Q. Chen ◽  
S.S. Schreiber ◽  
R.D. Brinton
Keyword(s):  
Mrna Expression ◽  
Oxytocin Receptor ◽  
Receptor Mrna

scholarly journals Dynamic Changes in Oxytocin Receptor Expression and Activation at Parturition in the Rat Brain

Endocrinology ◽  
2007 ◽  
Vol 148 (10) ◽  
pp. 5095-5104 ◽  
Author(s):  
Simone L. Meddle ◽  
Valerie R. Bishop ◽  
Effimia Gkoumassi ◽  
Fred W. van Leeuwen ◽  
Alison J. Douglas
Keyword(s):  
Mrna Expression ◽  
Paraventricular Nucleus ◽  
Maternal Behavior ◽  
Brain Regions ◽  
Oxytocin Receptor ◽  
Lateral Septum ◽  
Receptor Expression ◽  
Olfactory Bulbs ◽  
Bed Nucleus ◽  
The Brain

Oxytocin plays a pivotal role in rat parturition, acting within the brain to facilitate its own release in the supraoptic nucleus (SON) and paraventricular nucleus, and to stimulate maternal behavior. We investigated oxytocin receptor (OTR) expression and activation perinatally. Using a 35S-labeled riboprobe complementary to OTR mRNA, OTR expression was quantified in proestrus virgin, 21- and 22-day pregnant, parturient (90 min. from pup 1 birth), and postpartum (4–12 h from parturition) rats. Peak OTR mRNA expression was observed at parturition in the SON, brainstem regions, medial preoptic area (mPOA), bed nucleus of the stria terminalis (BnST), and olfactory bulbs, but there was no change in the paraventricular nucleus and lateral septum. OTR mRNA expression was increased on the day of expected parturition in the SON and brainstem, suggesting that oxytocin controls the pathway mediating input from uterine signals. Likewise, OTR mRNA expression was increased in the mPOA and BnST during labor/birth. In the olfactory bulbs and medial amygdala, parturition induced increased OTR mRNA expression compared with pre-parturition, reflecting their immediate response to new stimuli at birth. Postpartum OTR expression in all brain regions returned to levels observed in virgin rats. Parturition significantly increased the number of double-immunolabeled cells for Fos and OTR within the SON, brainstem, BnST, and mPOA regions compared with virgin rats. Thus, there are dynamic region-dependent changes in OTR-expressing cells at parturition. This altered OTR distribution pattern in the brain perinatally reflects the crucial role oxytocin plays in orchestrating both birth and maternal behavior.

Regulation of oxytocin receptor gene expression in sheep: tissue specificity, multiple transcripts and mRNA editing

Reproduction ◽  
2000 ◽  
pp. 187-200 ◽  
Author(s):  
HC Feng ◽  
M Bhave ◽  
RJ Fairclough
Keyword(s):  
Gene Expression ◽  
Mrna Expression ◽  
Receptor Gene ◽  
Oxytocin Receptor ◽  
Oestrous Cycle ◽  
Receptor Expression ◽  
Mrna Editing ◽  
Oxytocin Receptor Gene ◽  
Receptor Mrna ◽  
Receptor Gene Expression

The increase in uterine oxytocin receptor concentrations over the late luteal phase of the oestrous cycle in sheep is thought to play an important role in the regulation of the duration of the cycle by facilitating the effect of oxytocin on uterine prostaglandin release. Experiments indicated that oxytocin receptor mRNA expression in the endometrium was high at oestrus compared with at days 2, 7 and 12 of the oestrous cycle. The amount of oxytocin receptor mRNA expression in the pituitary gland did not show any significant differences during the oestrous cycle. Oxytocin receptor cDNA was obtained and characterized from ovine uterine endometrium on day 15 of the oestrous cycle, using RT-PCR techniques, to study the mechanisms underlying the resolution of oxytocin receptor expression. The cDNA sequence for the oxytocin receptor gene in sheep was found to be similar to that described previously, except for a difference of seven nucleotides. These nucleotide differences resulted in changes in four of the deduced amino acids in the oxytocin receptor sequence. The heterogeneity of the different sized oxytocin receptor transcripts in sheep is due, at least in part, to the alternative use of polyadenylation sites. Northern hybridization confirmed that the oxytocin receptor gene is expressed in ovine corpus luteum. The investigations on oxytocin receptor gene expression indicate that the patten of oxytocin receptor gene expression in sheep is not only tissue-specific, but also highly function-related. Evidence was obtained of mRNA editing in both the coding and the 3'-untranslated (3'UTR) regions of oxytocin receptor gene transcripts in ovine endometrium; this was the first demonstration of this phenomenon for oxytocin receptor mRNA. The present results indicate that the observed differences in oxytocin receptor mRNA sequences for the different oxytocin receptor populations in endometrium are due to mRNA editing. mRNA editing of oxytocin receptor transcripts may be reflected in changes in the amino acid composition of the carboxyl terminus of the receptor, which would explain the differences in the observed responses to an oxytocin challenge.

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