scholarly journals Association between microtubules and symbiotic fungal hyphae in protocorm cells of the orchid species, Spiranthes sinensis

1998 ◽  
Vol 140 (4) ◽  
pp. 715-722 ◽  
Author(s):  
YUKARI UETAKE ◽  
R. LARRY PETERSON
Keyword(s):  
Orchid Species ◽  
Fungal Hyphae ◽  
Spiranthes Sinensis

scholarly journals Pollinium development in Spiranthes sinensis (Pers.) Ames.and Cymbidium pendulum SW: a comparative study

2014 ◽  
Vol 42 (2) ◽  
pp. 307-314 ◽  
Author(s):  
Ravi Kant ◽  
Mohammad Musharof Hossain ◽  
Lucky K Attri
Keyword(s):  
Electron Microscopy ◽  
Pollen Grains ◽  
Anther Development ◽  
Anther Wall ◽  
Terrestrial Orchid ◽  
Orchid Species ◽  
Meristematic Cells ◽  
Phylogenetic Reconstructions ◽  
Spiranthes Sinensis ◽  
Scanning Electron

Comparative studies on the early anther development, anther wall differentiation, pollinium development and pollen grains surface features in Spiranthes sinensis (Pers.) Ames. (a terrestrial orchid) and Cymbidium pendulum Sw. (an epiphytic orchid) using light and scanning electron microscopy were described. Anther primordium initiated as a homogenous mass of meristematic cells which developed two thecae, each with a group of archesporial cells of dense cytoplasmic contents. The sporogenous cells of the hypodermal layer formed the anther wall which was 4-5 layered in S. sinensis and 7-layered in C. pendulum. The rare feature of 2-layered endothecium in S. sinensis and a 2-layered tapetum in C. pendulum was reported. Papillate epidermis was observed in C. pendulum. A complete septum of sterile cells in S. sinensis and partial in C. pendulum was observed. Pollen grains were mono-aperturate, reticulate and shed as tetrads in S. sinensis while either monoaperturate or inaperturate, tectate pollen grains and dispersed as hard pollinia in C. pendulum. This is a see-through information for accurate classification and phylogenetic reconstructions of orchid species. DOI: http://dx.doi.org/10.3329/bjb.v42i2.18035 Bangladesh J. Bot. 42(2): 307-314, 2013 (December)

Changes in actin filament arrays in protocorm cells of the orchid species, Spiranthes sinensis, induced by the symbiotic fungus Ceratobasidium cornigerum

1997 ◽  
Vol 75 (10) ◽  
pp. 1661-1669 ◽  
Author(s):  
Yukari Uetake ◽  
R. Larry Peterson
Keyword(s):  
Confocal Microscopy ◽  
Actin Filament ◽  
Actin Filaments ◽  
Laser Scanning ◽  
Laser Scanning Confocal Microscopy ◽  
Cell Cytoplasm ◽  
Symbiotic Fungus ◽  
Scanning Confocal Microscopy ◽  
Fungal Hyphae ◽  
Spiranthes Sinensis

Seeds of the terrestrial orchid, Spiranthes sinensis, were germinated in vitro in association with the symbiotic fungus, Ceratobasidium cornigerum. Resulting colonized protocorms were prepared for light microscopy, transmission electron microscopy, and fluorescence labelling of actin filaments for examination with laser scanning confocal microscopy. Fungal hyphae invaded the suspensor end of embryos, formed typical hyphal coils (pelotons) within parenchyma cells, and then underwent lysis resulting in degraded hyphal masses. Hyphae and hyphal masses were enveloped by host-derived membrane. Changes in actin filament arrays accompanied fungal colonization. Uncolonized cells had a network of actin filaments and actin bundles (cables) located in the cortical region of the cell cytoplasm; some of these were associated with the nucleus and amyloplasts. Although actin filament arrays were still present in protocorm cell cytoplasm during fungal entry and peloton formation, most of the cortical network disappeared and instead actin filaments radiated from the periphery of developing pelotons towards the cell wall. Degraded hyphal masses also had actin filament arrays associated with them, again radiating toward the cell periphery; a network of cortical actin filaments reappeared in the protocorm cell cytoplasm at this stage. Actin filaments did not appear to have a close physical association with fungal hyphae except in the epidermal hairs that developed from protocorms; this differs from our previous observations on microtubules in this system. Key words: actin, actin filaments, orchids, mycorrhizas, laser scanning confocal microscopy.

Pertumbuhan Dan Perkembangan Anggrek Dendrobium anosmum Pada Media Kultur In Vitro Dengan Beberapa Konsentrasi Air Kelapa

Agrologia ◽  
2018 ◽  
Vol 1 (1) ◽  
Author(s):  
S. Tuhuteru ◽  
Meity L Hehanussa ◽  
Simon H.T Raharjo
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Water Concentration ◽  
Medium Composition ◽  
Coconut Water ◽  
Orchid Species ◽  
Randomized Design ◽  
Wet Weight ◽  
Completely Randomized Design

Dendrobium anosmum is one of natural orchids in Indonesia. Optimization of medium composition for orchid propagation through in vitro culture is necessary to enhance propagule multiplication capabilities and quality. This study was aimed to study the influence of concentration of coconut water in culture medium on in vitro growth and development of D. anosmum orchid species and to determine the optimal coconut water concentration in culture media.  The experiment were arranged in a Completely Randomized Design with four treatments and eight replications. The treatments consisted of the addition of coconut water with concentrations: 0 ml•l -1 (control), 50 ml•l-1, 100 ml•l-1 and 150 ml•l-1. The results showed that addition of coconut water in culture medium gave different effect on shoot growth and multiplication of D. anosmum orchids.  Coconut water concentration of 100 ml•l-1 was the best concentration for growth and multiplication of D. anosmum orchids, based on both shoots and roots growth, plantlet height and wet weight.

Distribution Models and Spatial Analyses Provide Robust Assessments of Conservation Status of Orchid Species in Colombia: The Case of Lephantes mucronata

2020 ◽  
Vol 25 (1) ◽  
pp. 111
Author(s):  
Juan Sebastián Moreno ◽  
Stephania Sandoval-Arango ◽  
Rubén Darío Palacio ◽  
Nestor Fabio Alzate ◽  
Milton Rincón ◽  
...  
Keyword(s):  
Conservation Status ◽  
Spatial Analyses ◽  
Distribution Models ◽  
Orchid Species

New orchid species (Orchidaceae) in the flora of Laos

2019 ◽  
pp. 25-40
Author(s):  
L. V. Averyanov ◽  
Khang Sinh Nguyen ◽  
T. V. Maisak ◽  
Hiep Tien Nguyen ◽  
N. L. Orlov ◽  
...  
Keyword(s):  
New Species ◽  
Related Species ◽  
Field Studies ◽  
Regional Literature ◽  
Orchid Species ◽  
Two New Species

This paper continues the publication of new data obtained during field studies in remote and poorly studied regions of Laos in 2015–2018. It provides descriptions of two new species (Bulbophyllum brachyscapum Aver. and Sunipia saccata Aver.), emended descriptions of two previously published but insufficiently known species (Cleisostoma verrucosum Aver., Oberonia vesiculifera Aver.) and reports of eight species newly recorded in the flora of Laos (Biermannia calcarata Aver., Bulbophyllum farreri (W. W. Sm.) Seidenf., Didymoplexiella trichechus (J. J. Sm.) Garay, Eria eriopsidobulbon C. S. P. Parish et Rchb. fil., Habenaria ciliolaris Kraenzl., Liparis caudata Aver. et K. S. Nguyen, Odontochilus umbrosus (Aver.) Ormerod, and Porpax ustulata (C. S. P. Parish et Rchb. fil.) Rolfe). Appropriate citations of regional literature, colour illustrations, data on types and distribution, descriptions, etymology, as well as notes on taxonomy and related species are provided for the studied species.

Bulbophyllum putaoensis (Orchidaceae: Epidendroideae; Malaxideae), a new species from Kachin State, Myanmar

Phytotaxa ◽  
2017 ◽  
Vol 305 (1) ◽  
pp. 57 ◽  
Author(s):  
QIANG LIU ◽  
SHI-SHUN ZHOU ◽  
REN LI ◽  
MING-XIA ZHANG ◽  
MYINT ZYAW ◽  
...  
Keyword(s):  
New Species ◽  
Orchid Species ◽  
Rugged Terrain ◽  
A New Species ◽  
New Distribution

The orchid flora of Myanmar is highly diverse but as yet poorly known in the continental Asia, which is largely a result of periods of past instability and political isolation of the country. Also the remoteness of many orchid-rich areas and the difficulties of investigation in rugged terrain have also played a role (Ormerod & Kumar, 2003; Kurzweil & Lwin, 2014). According to recent estimates about 800 orchid species are distributed in Myanmar (Kurzweil & Lwin, 2014), which were probably underestimated. Many new distribution records and new species have been published in the last few years (Ormerod, 2002, 2006, 2012; Ormerod & Kumar, 2003, 2008; Ormerod & Wood, 2010; Nyunt, 2006; Kurzweil et al., 2010; Kurzweil & Lwin, 2012a,b; Tanaka et al. 2011).

scholarly journals Territorial Protection of Rare Orchid Species (Orchidaceae) in the Nizhny Novgorod Region

2021 ◽  
Vol 688 (1) ◽  
pp. 012002
Author(s):  
S V Bakka ◽  
N Yu Kiseleva ◽  
A A Schestakova ◽  
S P Urbanavichute
Keyword(s):  
Orchid Species ◽  
Nizhny Novgorod Region

scholarly journals Genome Size Diversity in Rare, Endangered, and Protected Orchids in Poland

Genes ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 563
Author(s):  
Monika Rewers ◽  
Iwona Jedrzejczyk ◽  
Agnieszka Rewicz ◽  
Anna Jakubska-Busse
Keyword(s):  
Genome Size ◽  
Species Identification ◽  
Dna Content ◽  
Size Estimation ◽  
Orchid Species ◽  
Infraspecific Taxon ◽  
2C Dna Content ◽  
Plant Families ◽  
Liparis Loeselii ◽  
Identification And Characterization

Orchidaceae is one of the largest and the most widespread plant families with many species threatened with extinction. However, only about 1.5% of orchids’ genome sizes have been known so far. The aim of this study was to estimate the genome size of 15 species and one infraspecific taxon of endangered and protected orchids growing wild in Poland to assess their variability and develop additional criterion useful in orchid species identification and characterization. Flow cytometric genome size estimation revealed that investigated orchid species possessed intermediate, large, and very large genomes. The smallest 2C DNA content possessed Liparis loeselii (14.15 pg), while the largest Cypripedium calceolus (82.10 pg). It was confirmed that the genome size is characteristic to the subfamily. Additionally, for four species Epipactis albensis, Ophrys insectifera, Orchis mascula, Orchis militaris and one infraspecific taxon, Epipactis purpurata f. chlorophylla the 2C DNA content has been estimated for the first time. Genome size estimation by flow cytometry proved to be a useful auxiliary method for quick orchid species identification and characterization.

Influence of fungi on multiplication and distribution of the pine wood nematode, Bursaphelenchus xylophilus, in axenized Pinus thunbergii cuttings

Nematology ◽  
2005 ◽  
Vol 7 (6) ◽  
pp. 809-817 ◽  
Author(s):  
Kazuo Suzuki ◽  
Daisuke Sakaue ◽  
Toshihiro Yamada ◽  
Yu Wang
Keyword(s):  
Population Size ◽  
Early Stage ◽  
Bursaphelenchus Xylophilus ◽  
Pinus Thunbergii ◽  
Disease Development ◽  
Pine Wood Nematode ◽  
Pine Wood ◽  
Inoculation Site ◽  
Fungal Hyphae ◽  
Close Relationship

AbstractInfluence of fungi on multiplication and distribution of the pine wood nematode (PWN), Bursaphelenchus xylophilus, was investigated in Pinus thunbergii cuttings. Axenized nematodes and/or one of two fungi isolated from healthy and PWN-killed P. thunbergii were inoculated together into autoclaved cuttings. A close relationship between the existence and distribution of fungal hyphae, and the multiplication and distribution of PWN was observed. The PWN did not multiply when only axenized nematodes were inoculated in the absence of fungi. When fungi were present, PWN population size increased markedly. The number of nematodes was high at sites where fungal hyphae were distributed. It is suggested that the restriction of a large portion of the nematode population near the inoculation site during the early stage of disease development is closely related to restricted distribution of fungal hyphae.

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