scholarly journals In vitro and ex vitro rooting of Spiraea betulifolia subsp. aemiliana (Rosaceae), an ornamental shrub

2020 ◽  
Vol 24 ◽  
pp. 00058
Author(s):  
Dinara Muraseva ◽  
Vera Kostikova
Keyword(s):  
Ms Medium ◽  
Pulse Treatment ◽  
Root Number ◽  
Ex Vitro ◽  
Effective Technique ◽  
Nutrient Media ◽  
Half Strength ◽  
Regenerated Plantlets ◽  
Ornamental Shrub

Two methods of rhizogenesis – in vitro and ex vitro of Spiraea betulifolia subsp. aemiliana (C.K. Schneid.) H. Hara microshoots have been compared. Pulse treatment of microshoots with aqueous solutions of 4% “Heteroauxin” or 2% succinic acid (ex vitro rooting) did not effective -rooting frequency ranged from 3 to 19%. It was established that the in vitro rooting on nutrient media supplemented with auxins was a more effective technique, providing a high percentage of rooted microshoots. The use of half- strength MS medium supplemented with 0.1 μM indolyl-3-butyric acid (rooting frequency 8 8%, root number 3.5 ± 0.3 per plantlet) was found to be the most efective for in vitro rooting. The in vitro rooted regenerated plantlets were successfully acclimatized with 55% of survival rate.

scholarly journals In vitro multiplication, micromorphological studies and ex vitro rooting of Hybanthus enneaspermus (L.) F. Muell. – a rare medicinal plant

2018 ◽  
Vol 77 (1) ◽  
pp. 80-87 ◽  
Author(s):  
Mahipal S. Shekhawat ◽  
M. Manokari
Keyword(s):  
Medicinal Plant ◽  
Large Scale ◽  
Nodal Segments ◽  
Ms Medium ◽  
Ex Vitro ◽  
Culture Bottle ◽  
Hybanthus Enneaspermus ◽  
Half Strength

AbstractHybanthus enneaspermusis a rare medicinal plant. We defined a protocol for micropropagation,ex vitrorooting of cloned shoots and their acclimatization. Surface-sterilized nodal segments were cultured on Murashige and Skoog (MS) medium with different concentrations of 6-benzylaminopurine (BAP) and kinetin (Kin). Medium supplemented with 1.5 mg L−1BAP was found optimum for shoot induction from the explants and 6.4±0.69 shoots were regenerated from each node with 97% response. Shoots were further proliferated maximally (228±10.3 shoots per culture bottle with 7.5±0.43 cm length) on MS medium augmented with 1.0 mg L−1each of BAP and Kin within 4–5 weeks. The shoots were rootedin vitroon half strength MS medium containing 2.0 mg L−1indole-3 butyric acid (IBA). The cloned shoots were pulse-treated with 300 mg L–1 of IBA and cultured on soilrite® in a greenhouse. About 96% of the IBA-pulsed shoots rootedex vitroin soilrite®, each shoot producing 12.5±0.54 roots with 5.1±0.62 cm length. Theex vitrorooted plantlets showed a better rate of survival (92%) in a field study thanin vitrorooted plantlets (86%). A comparative foliar micromorphological study ofH. enneaspermuswas conducted to understand the micromorphological changes during plant developmental processes fromin vitrotoin vivoconditions in terms of variations in stomata, vein structures and spacing, and trichomes. This is the first report onex vitrorooting inH. enneaspermusand the protocol can be exploited for conservation and large-scale propagation of this rare and medicinally important plant.

scholarly journals IN VITRO REGENERATION AND MASS PROPAGATION OF AZIMA TETRACANTHA [LAM.] FROM THE LEAF EXPLANTS THROUGH CALLUS CULTURE

2017 ◽  
Vol 4 (2) ◽  
pp. 52-56
Author(s):  
Mallika Devi T
Keyword(s):  
Callus Induction ◽  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Shoot Formation ◽  
Ms Medium ◽  
Apical Leaf ◽  
Half Strength ◽  
Regenerated Plantlets ◽  
Azima Tetracantha

In the present study the protocol for callus induction and regeneration in Azima tetracantha has been developed in culture medium. The young apical leaf explants were used for callus induction on MS medium containing BAP and NAA at 1.0 and 0.4mgl-1 respectively showed maximum callus induction (73%). The amount of callus responded for shoot formation (74%) was obtained in the MS medium containing BAP (1.5 mgl-1) and NAA (0.3mgl-1).The elongated shoots were rooted on half strength medium supplemented with IBA (1.5 mgl-1) and Kn (0.4 mgl-1) for shoots rooted. Regenerated plantlets were successfully acclimatized and hardened off inside the culture and then transferred to green house with better survival rate.

scholarly journals Micropropagation of Achillea millefolium L. on half-strength ms medium and direct rooting and acclimatization of microshoots in hydroponic culture

2015 ◽  
pp. 99-112
Author(s):  
Marija Markovic ◽  
Dragana Skocajic ◽  
Mihailo Grbic ◽  
Matilda Djukic ◽  
Dragica Obratov-Petkovic ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Nutrient Solution ◽  
Ph Value ◽  
Ex Vitro Rooting ◽  
In Vitro Rooting ◽  
Efficient Production ◽  
Ms Medium ◽  
Ex Vitro ◽  
Half Strength

The aim of this study was to determine the possibility of micropropagation of the medicinal plant A. millefolium on half-strength MS medium and ex vitro rooting and acclimatization of the obtained microshoots in hydroculture in order to establish an efficient production method. Two explant types were used: basal and terminal cuttings, and better results were achieved when terminal cuttings were used. The development of shoots in the multiplication phase was successful with a regeneration percentage of 100%. Ex vitro rooting in a modified Hoagland nutrient solution was successful (83%), but the percentage of in vitro rooting on half-strength MS medium without hormones was higher (95%). However, bearing in mind that mass production of A. millefolium is more efficient when the phase of in vitro rooting is excluded, this method could be recommended for commercial propagation of this medicinal plant. It is necessary to conduct additional research in order to optimize the composition, EC and pH value of the hydroponic nutrient solution.

scholarly journals Effect of Seed Orientation and Medium Strength on In vitro Germination of Pterocarpus marsupium Roxb.

2013 ◽  
Vol 5 (4) ◽  
pp. 476-479 ◽  
Author(s):  
Yogeshwar MISHRA ◽  
Rimi RAWAT ◽  
Brajesh NEMA ◽  
Fatima SHIRIN
Keyword(s):  
Shoot Length ◽  
Ms Medium ◽  
Root Number ◽  
Pterocarpus Marsupium ◽  
Shoot Number ◽  
Medium Strength ◽  
Half Strength ◽  
Varied Response ◽  
Strength Medium

A method was developed for optimization of In vitro germination of an economically important timber-yielding multipurpose tree, Pterocarpus marsupium Roxb. The seeds inoculated in different orientation on different strengths of MS medium without any hormones showed varied response to the seed positions. The seeds inoculated on half strength medium in horizontal position recorded to produce a maximum germination (78.23%), shoot number (0.86) and root number (7.99). However, a maximum of shoot length of 3.67 cm was recorded in the quarter strength medium in the seeds inoculated in vertical down position, which was significantly higher than other media strength and positions. Our results indicate that the seed orientation including medium strength have tremendous effect on germination and seeds inoculated horizontally on half strength MS medium can be utilized to enhance In vitro seed germination of Pterocarpus marsupium.

scholarly journals Regeneration of Ficus glomerata Roxb., using shoot tips and nodal explants

1970 ◽  
Vol 39 (1) ◽  
pp. 47-50 ◽  
Author(s):  
AKM Sayeed Hassan ◽  
Rahima Khatun
Keyword(s):  
Survival Rate ◽  
Shoot Proliferation ◽  
Shoot Tips ◽  
Nodal Explants ◽  
Ms Medium ◽  
Shoot Induction ◽  
Half Strength ◽  
Regenerated Plantlets ◽  
Ficus Glomerata

Shoot tips and nodal explants from in vitro growing seedlings of Ficus glomerata Roxb. (Moraceae). showed best shoot induction (88%) on MS medium supplemented with 0.5 mg/l BAP, where maximum number of shoots were produced per culture. In vitro raised shoots rooted well on half strength of MS medium with 2.0 mg/l IBA + 0.1 mg/l NAA. The survival rate of regenerated plantlets was 82%. Key words: Ficus glomerata Roxb.; Shoot proliferation; Micropropagation; Acclimatization DOI: 10.3329/bjb.v39i1.5525Bangladesh J. Bot. 39(1): 47-50, 2010 (June)

scholarly journals Nutritional requirements for germination and in vitro development of three Orchidaceceae species in the southern Brazilian Amazon

2018 ◽  
Vol 24 (2) ◽  
pp. 87-94 ◽  
Author(s):  
Viviane Luiza Hunhoff ◽  
Lais Alves Lage ◽  
Ednamar Gabriela Palú ◽  
Willian Krause ◽  
Celice Alexandre Silva
Keyword(s):  
Activated Charcoal ◽  
Culture Media ◽  
Leaf Number ◽  
Alternative Form ◽  
Ms Medium ◽  
Root Number ◽  
Shoot Height ◽  
Full Strength ◽  
Half Strength

Tissue culture is an alternative form of producing healthy, vigorous and regular plants on a large scale. The purpose of this study was to evaluate the most efficient culture medium for in vitro plantlet germination and development of three Orchidaceae species. Seeds disinfested of three species were dispersed in distilled water and dripped into basic Murashige and Skoog (MS) medium. The experimental design was completely randomized in a factorial 3 x 4 (three species x four culture media), with 5 replications. Four treatments were established: (1) full-strength MS medium, with the full nutrient concentration (MSØ), (2) full-strength MS medium plus 0.3% activated charcoal (MSØ ACh), (3) half- strength MS medium (½ MS) and (4) half- strength MS medium with 0.3 % activated charcoal (½ MS ACh). Germination was evaluated after 15, 20, 25, 30, and 60 days. The shoot height, leaf number and length, root number and length of plantlets of the three studied species were assessed. In A. variegata, 73% germinated after 60 days in ½ MS ACh medium. In the same period, 100% of E. viparum and S. gloriosa seeds germinated in MSØ ACh medium. The plant height, leaf number and length, root number and length were significantly higher for the species A. variegata and E. viviparum in MSØ ACh medium. The culture media ½ MS and MSØ with addition of activated charcoal favored in vitro germination for the three orchid species of this study.

scholarly journals In Vitro Propagation And Rejuvenation of Senescent Maternal Plant of Ardisia Crenata Var. Bicolor (Primulaceae)

2021 ◽  
Author(s):  
Xingmei Ai ◽  
Yonghui Wen ◽  
Chao Wang
Keyword(s):  
Wild Population ◽  
Shoot Proliferation ◽  
Naphthaleneacetic Acid ◽  
Nodal Segment ◽  
Ms Medium ◽  
Shoot Initiation ◽  
Half Strength ◽  
Planting Materials ◽  
Ornamental Shrub

Abstract Ardisia crenata var. bicolor is an ornamental shrub, owing to its declined wild population, recalcitrant seeds and few high-quality cuttings, the main objective of this study was to optimize an in vitro propagation protocol by using tip shoot and nodal segment as explants from senescent plant. Explants were sterilized and cultured on Muraghige and Skoog medium contained 1.0 mg·L-1 benzylaminopurine and 0.05 mg·L-1 1-naphthaleneacetic acid for shoot initiation. For shoot proliferation, explants were cultured on MS medium with 1.0 mg·L-1 BAP, 0.1 mg·L-1 NAA, and 0.5 mg·L-1 kinetin, and the proliferation coefficient were 3.1 and 2.5. Rooting was achieved by two explants in half-strength MS medium containing 0.5 mg·L-1 indole-3-butyric acid + 0.1 mg·L-1 or 0.2 mg·L-1 NAA, and 0.5 g·L-1 activated charcoal. The highest rooting rate were 72.7% and 65.1% with the highest mean number of roots (4.2 and 2.8, respectively). After acclimatization, 83.3% and 81.2% of plants were survived in the greenhouse. The plant can be rejuvenated via in vitro propagation and provide a reference for supplying the planting materials quickly with an uniform genotype.

scholarly journals Micropropagation of an important medicinal herb Eclipta alba (L.) Hassk.

2016 ◽  
Vol 4 (1) ◽  
pp. 61-69 ◽  
Author(s):  
S Yesmin ◽  
A Hashem ◽  
MS Islam
Keyword(s):  
Morphological Variation ◽  
Multiple Shoots ◽  
Nodal Segments ◽  
Induction Medium ◽  
Root Induction ◽  
Ms Medium ◽  
Eclipta Alba ◽  
Half Strength ◽  
Regenerated Plantlets

Nodal segments from naturally grown Eclipta alba (L.) Hassk.were used as explants for organogenesis. Multiple shoots were obtained from the explants cultured on MS medium supplemented with various concentrations of BAP and Kn alone or in combination with NAA and IAA. Maximum number of multiple shoots (18.40±0.67) were induced on MS medium supplemented with 1.0 mg/l BAP and 0.1mg/NAA. In vitro raised shoots were cultured onto half and full strength MS medium supplemented with different concentration of IBA, IAA and NAA. The best root induction medium was found to be half strength MS containing 0.1 mg/l IBA where 96% shoots rooted. Regenerated plantlets grew normally without showing any morphological variation and flowered after 45 days of transplantation.Jahangirnagar University J. Biol. Sci. 4(1): 61-69, 2015 (June)

scholarly journals In Vitro Regeneration of Dendrobium sp. of Orchid Using Leaf Tip as Explant

2016 ◽  
Vol 8 (2) ◽  
pp. 75-78
Author(s):  
K Goswami ◽  
S Yasmin ◽  
KM Nasiruddin ◽  
F Khatun ◽  
J Akte
Keyword(s):  
In Vitro Regeneration ◽  
Plantlet Regeneration ◽  
Shoot Length ◽  
Ms Medium ◽  
Fresh Weight ◽  
Half Strength ◽  
Maximum Root Length ◽  
Maximum Root ◽  
Regenerated Plantlets

Plant growth regulators (PGRs) namely, 2,4-D, NAA and BAP were added into Murashige and Skoog (MS) medium to observe the effect of PGRs on the growth and development of Dendrobium sp. orchid. Leaf tips of Dendrobium sp. were used as explants and inoculated on MS medium supplemented with 2, 4 D (0, 0.5, 2.5, 5, 10 mgL?1) for development of PLBs. The maximum PLBs formation (90%) and the maximum number of PLBs (16.00) were observed in 10 mgL?1 2, 4-D into MS medium after 60 days of culture. Subcultured PLBs were inoculated on MS medium supplemented with different combinations of NAA (0, 0.5, 2.5, 5 mgL?1) and BAP (0, 0.5, 2.5, 5 mgL?1) for shoot regeneration. The maximum number of shoot (11.00), the highest fresh weight (0.6233g) and the highest shoot length (3.613 cm) were observed in 0.5 mgL?1 NAA + 0.5 mgL?1 BAP after 60 days of culture. Even, the maximum number of root (4.00), the maximum root length (1.627cm) and the maximum plantlet regeneration percentage (93.33%) were observed with the combined effect of 0.5 mg NAA and 0.5 mg BAP after 60 days of culture. Finally, regenerated plantlets were transferred into half strength MS medium to obtain plants.J. Environ. Sci. & Natural Resources, 8(2): 75-78 2015

scholarly journals Micropropagation of yardlong bean (Vigna unguiculata (L.) Walsp. ssp. Sesquipedalis L. Verdc.) through in vitro culture

2018 ◽  
Vol 44 (2) ◽  
pp. 345-350
Author(s):  
I Jahan ◽  
N Alam ◽  
PK Roy
Keyword(s):  
Vigna Unguiculata ◽  
Shoot Multiplication ◽  
Leaf Segment ◽  
Ms Medium ◽  
Plant Type ◽  
Half Strength ◽  
Yardlong Bean ◽  
Parental Lines ◽  
Regenerated Plantlets

An in vitro propagation protocol has been established for yardlong bean (Vigna unguiculata ssp. sesquipedalis L. Walp. Verdc.) using shoot tip, nodal and leaf segment explants of the superior plant type obtained from crossing of two selected parental lines. Explants were cultured on MS medium supplemented with different concentrations and combinations of BAP, Kn and Zeatin and NAA, IAA, IBA. The highest percentage (86) of shoot regeneration with maximum shoots per culture (12 ± 0.43) was obtained from nodal explants on MS medium supplemented with 0.5 mg/l BAP and 0.2 mg/l NAA. Addition of 10% coconut water and 75 mg/l urea to the medium enhanced shoot multiplication and elongation, respectively. Rooting of the regenerated shoots was optimum on half-strength MS enriched with 3.0 mg/l IBA. Regenerated plantlets were successfully acclimatized. After acclimation, plantlets were transplanted in the experimental field where the survival rate was 80%.

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