Identification and Characterization of Protamine1 Gene in Aceh Cattle

Naturally, the fertility of Aceh cattle has declined and caused huge losses in the development of Aceh cow germplasm. Several genetic-based studies have been carried out in Aceh cattle, including characteristics of genetic diversity using microsatellite DNA and its relationship to body weight in Aceh cows, but there is no research on genomics that is closely related to fertility, especially fertility in aceh cows. Previous studies have revealed a correlation between fertility with the expression of several genes involved in spermatogeneses such as Protamines (PRM 1, 2, and 3) and Transition Nuclear Proteins (TNPS 1 and 2). This study aims to identify the PRM1 gene that plays an important role in spermatogenesis and the fertility status of Aceh cattle. This research was carried out using 10 Aceh cattle testes taken from slaughterhouses, followed by DNA isolation, amplification of the PRM1 gene, DNA sequencing, and DNA base sequence analysis. The result showed that the nucleotide sequences of aceh cattle PRM1 gene did not differ from the PRM1 gene from Bos taurus and Bos indicus in the GeneBank. Analysis of protein sequences showed that PRM1 in Aceh cattle sperm is rich in the amino acid arginine, which is 36.92%. From this study, it can be concluded that the PRM1 gene from Aceh cattle is identical to the PRM1 gene from Bos taurus and Bos indicus both at the DNA sequence level and at the protein sequence.

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Identification and characterization of novel xylose isomerases from a Bos taurus fecal metagenome

Applied Microbiology and Biotechnology ◽

10.1007/s00253-019-10161-1 ◽

2019 ◽

Vol 103 (23-24) ◽

pp. 9465-9477 ◽

Cited By ~ 1

Author(s):

Ruiqi Tang ◽

Peiliang Ye ◽

Hal S. Alper ◽

Zhanying Liu ◽

Xinqing Zhao ◽

...

Keyword(s):

Bos Taurus ◽

Identification And Characterization

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Identification and characterization of microsatellite DNA markers developed in ide Leuciscus idus and Siberian roach Rutilus rutilus

Molecular Ecology Notes ◽

10.1046/j.1471-8286.2003.00577.x ◽

2004 ◽

Vol 4 (1) ◽

pp. 86-88 ◽

Cited By ~ 16

Author(s):

Anna Barinova ◽

Elena Yadrenkina ◽

Masamichi Nakajima ◽

Nobuhiko Taniguchi

Keyword(s):

Dna Markers ◽

Microsatellite Dna ◽

Rutilus Rutilus ◽

Roach Rutilus Rutilus ◽

Microsatellite Dna Markers ◽

Leuciscus Idus ◽

Identification And Characterization

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Phenotypic characterization of the Saudi Arabian Hassawi cattle breed

Animal Genetic Resources Information ◽

10.1017/s1014233900000900 ◽

1997 ◽

Vol 21 ◽

pp. 35-42 ◽

Cited By ~ 4

Author(s):

T. A. Mohammed

Keyword(s):

Bos Taurus ◽

Bos Indicus ◽

Cattle Breed ◽

Farming System ◽

Phenotypic Characterization ◽

Zebu Cattle ◽

Body Conformation ◽

High Feed ◽

Farming Families

SummaryHassawi cattle breed is a mix of Bos indicus and Bos taurus. The cattle are raised in the Eastern province of the country by farming families in mixed farming system. The breed numbers are declining very fast, from 10 449 head in 1986 to an estimated maximum of 4 500 head at present.The decrease is mainly due to replacement by exotic breeds, the indiscriminate crossing with these exotics, particularly in view of the scarcity of the Hassawi bulls for mating. Animals are small in size, mature body weight 210-270 kg for bulls and 150-200 kg for cows, quite uniform in colour (light red) and body conformation have conspicuously reduced dewlap and umbilical folds and relatively large hump. Animals are heat tolerant, sustain high feed intake under ambient temperature, resistant to many diseases prevailing in the region and cows have good mothering ability. Productivity of the breed in terms of meat and milk is low when compared to that of exotics in high input production environments, but reproduction performance excels that of temperate breeds and zebu cattle.Efforts should be made to stop the decline in the breed numbers and to conserve the breed as an asset for production under harsh environment.

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Whole genome characterization of autochthonous Bos taurus brachyceros and introduced Bos indicus indicus cattle breeds in Cameroon regarding their adaptive phenotypic traits and pathogen resistance

BMC Genetics ◽

10.1186/s12863-020-00869-9 ◽

2020 ◽

Vol 21 (1) ◽

Author(s):

Archile Paguem ◽

Babette Abanda ◽

Mbunkah Daniel Achukwi ◽

Praveen Baskaran ◽

Stefan Czemmel ◽

...

Keyword(s):

Bos Taurus ◽

Bos Indicus ◽

Pathogen Resistance ◽

Phenotypic Traits ◽

Whole Genome ◽

Genome Characterization ◽

Cattle Breeds

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Isolation, Identification and Characterization of Group A Rotavirus from a Chicken: the Inner Capsid Protein Sequence shows only a Distant Phylogenetic Relationship to Most Other Avian Group A Rotaviruses

Journal of Veterinary Medicine Series B ◽

10.1111/j.1439-0450.2005.00848.x ◽

2005 ◽

Vol 52 (5) ◽

pp. 211-213 ◽

Cited By ~ 10

Author(s):

M. Elschner ◽

H. Hotzel ◽

J. Reetz ◽

R. Diller ◽

P. Otto

Keyword(s):

Capsid Protein ◽

Phylogenetic Relationship ◽

Protein Sequence ◽

Capsid Protein Sequence ◽

Group A Rotavirus ◽

Group A Rotaviruses ◽

Group A ◽

Avian Group ◽

Identification And Characterization

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Characterization of Breeds of Bos Indicus and Bos Taurus Cattle for Maternal and Individual Traits

Journal of Animal Science ◽

10.2527/jas1985.6051165x ◽

1985 ◽

Vol 60 (5) ◽

pp. 1165-1174 ◽

Cited By ~ 8

Author(s):

K. E. Gregory ◽

J. C. M. Trail ◽

H. J. S. Marples ◽

J. Kakonge

Keyword(s):

Bos Taurus ◽

Bos Indicus ◽

Individual Traits

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A systematic bioinformatics approach for large-scale identification and characterization of host-pathogen shared sequences

BMC Genomics ◽

10.1186/s12864-021-07657-4 ◽

2021 ◽

Vol 22 (S3) ◽

Author(s):

Stephen Among James ◽

Hui San Ong ◽

Ranjeev Hari ◽

Asif M. Khan

Keyword(s):

Disease Surveillance ◽

Large Scale ◽

Sequence Data ◽

Protein Sequences ◽

Public Access ◽

Biological Databases ◽

Mortality And Morbidity ◽

Host Pathogen ◽

Identification And Characterization

Abstract Background Biology has entered the era of big data with the advent of high-throughput omics technologies. Biological databases provide public access to petabytes of data and information facilitating knowledge discovery. Over the years, sequence data of pathogens has seen a large increase in the number of records, given the relatively small genome size and their important role as infectious and symbiotic agents. Humans are host to numerous pathogenic diseases, such as that by viruses, many of which are responsible for high mortality and morbidity. The interaction between pathogens and humans over the evolutionary history has resulted in sharing of sequences, with important biological and evolutionary implications. Results This study describes a large-scale, systematic bioinformatics approach for identification and characterization of shared sequences between the host and pathogen. An application of the approach is demonstrated through identification and characterization of the Flaviviridae-human share-ome. A total of 2430 nonamers represented the Flaviviridae-human share-ome with 100% identity. Although the share-ome represented a small fraction of the repertoire of Flaviviridae (~ 0.12%) and human (~ 0.013%) non-redundant nonamers, the 2430 shared nonamers mapped to 16,946 Flaviviridae and 7506 human non-redundant protein sequences. The shared nonamer sequences mapped to 125 species of Flaviviridae, including several with unclassified genus. The majority (~ 68%) of the shared sequences mapped to Hepacivirus C species; West Nile, dengue and Zika viruses of the Flavivirus genus accounted for ~ 11%, ~ 7%, and ~ 3%, respectively, of the Flaviviridae protein sequences (16,946) mapped by the share-ome. Further characterization of the share-ome provided important structural-functional insights to Flaviviridae-human interactions. Conclusion Mapping of the host-pathogen share-ome has important implications for the design of vaccines and drugs, diagnostics, disease surveillance and the discovery of unknown, potential host-pathogen interactions. The generic workflow presented herein is potentially applicable to a variety of pathogens, such as of viral, bacterial or parasitic origin.

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Identification and characterization of microsatellite DNA markers developed in an endangered cyprinid of the Mekong River, the seven-line barb (Probarbus jullieni Sauvage, 1880)

Molecular Ecology Notes ◽

10.1111/j.1471-8286.2004.00702.x ◽

2004 ◽

Vol 4 (3) ◽

pp. 465-467 ◽

Cited By ~ 2

Author(s):

N. SUKUMASAVIN ◽

M. NAKAJIMA ◽

N. TANIGUCHI

Keyword(s):

Dna Markers ◽

Microsatellite Dna ◽

Mekong River ◽

Microsatellite Dna Markers ◽

Identification And Characterization

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Genotyping of the polymorphism within exon 3 of prolactin gene in various dairy breeds by PCR RFLP (Brief report)

Archives Animal Breeding ◽

10.5194/aab-51-298-2008 ◽

2008 ◽

Vol 51 (3) ◽

pp. 298-299 ◽

Cited By ~ 2

Author(s):

A. Ratna Kumari ◽

K. M. Singh ◽

K. J. Soni ◽

R. K. Patel ◽

J. B. Chauhan ◽

...

Keyword(s):

Mammary Gland ◽

Dairy Cattle ◽

Genetic Characterization ◽

Bos Taurus ◽

Bos Indicus ◽

Genotype Frequencies ◽

Prolactin Gene ◽

Pcr Rflp ◽

Transition Mutation

Abstract. In mammals, especially dairy cattle the prolactin has important functions like the development of mammary gland affecting milk yield and composition. It has been mapped to chromosome 23 in Bovine (HALLERMAN et al., 1988). A silent A→G transition mutation at the codon for amino acid 103 in exon 3 of bovine prolactin (bPRL) gene gives rise to a polymorphic Rsa I site, has become a popular genetic marker used for genetic characterization of cattle populations by means of PCR-RFLP (MITRA et al., 1995; CHRENEK et al., 1998; DYBUS, 2005). The present study reports on the genotype frequencies observed in various Bos taurus and Bos indicus dairy cattle breeds.

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