scholarly journals Chlorophyll fluorescence for classification of tomato fruits by their maturity stage

2020 ◽  
Vol 193 ◽  
pp. 01065
Author(s):  
M. A. Abdelhamid ◽  
Yu. A. Sudnik ◽  
H. J. Alshinayyin ◽  
Fatma Shaaban

Classification of tomato fruit and control of post-harvest maturation based on the stage of maturity at harvesting, are necessary to ensure the highest possible quality and marketability of the final ripe product. A method of sorting tomatoes to assess the degree of their maturation based on the control of their chlorophyll fluorescence induction is proposed. Tomatoes (Black baron) at five different stages of maturity were used. Variance analysis (ANOVA) was performed and the Duncan’s mean values were compared at a significance level of p ≤0.05. In addition, the correlation between the chlorophyll fluorescence induction parameters and the maturity of the same fruit was carried out using the statistical software SPSS 20.0. The general pattern of fluorescence induction analysis was revealed; as the fruit matures, the value of both maximum fluorescence induction (Fm) and the coefficient of specific photosynthetic activity of tomatoes (Kf) decreases. Both Fm and Kf exhibited a statistically significant difference (p ≤0.05) between stages of maturity. A very strong negative correlation between slow chlorophyll fluorescence induction parameters (Fm and Kf) and maturity were observed. Moreover, there is a strong positive correlation between Fm and Kf. In conclusion, the slow induction of chlorophyll fluorescence is a good indicator of the degree of maturity of tomato fruits and the proposed method had better reflected the actual ripening process of fruit per maturity stage.

Author(s):  
Yuriy A. Sudnik ◽  
Makhmud Abdelkhamid Abdelkhamid

The maturity of tomatoes serves as a basic parameter that determines their quality. Available maturity monitoring tools allow to measure the reflection coefficients of light from the surfaces of tomato fruits at certain wavelengths. These devices have the disadvantage of insufficient reliability in assessing the stages of maturity of tomatoes, they do not take into account their internal quality and structure. (Research purpose) The research purpose is in studying the dependence of the maturity of tomatoes on the level of slow induction of chlorophyll fluorescence. (Materials and methods) The article proposes a new method for separating tomato fruits according to their maturity, eliminating the inaccuracies of existing methods, based on the physical phenomenon of slow induction of chlorophyll fluorescence. During the experiment, three botanical varieties of tomatoes with different colors of mature fruits were used: “Alcazar” (red); “Wild rose” (pink); “Orange” (yellow). Five levels of maturity of single-sized tomato fruits were selected for each variety. Authors performed a variance analysis and comparison of the average values for the Duncan criterion with the significance level at α ≤ 0.05 using statistical analysis of the SPSS V. 20 software. (Results and discussion) The article presents general pattern for these tomato varieties: as the fruit matures, the value of their maximum fluorescence induction Fm and the coefficient of specific photosynthetic activity of tomatoes Kf decreases. The differences between the maturation stages with Fm and Kf are statistically significant at α ≤ 0.05. Regardless of the botanical variety, there are high negative correlations obtained for parameters of slow chlorophyll fluorescence induction, such as Fm and Kf with maturity. (Conclusions) The dependence of the decrease in the maximum fluorescence levels of chlorophyll with an increase in the maturity of tomato fruits was found. The proposed method allows to reliably separate tomato fruits with any color of mature fruits.


2010 ◽  
Vol 12 (4) ◽  
pp. 546-563 ◽  
Author(s):  
Esa Tyystjärvi ◽  
Michael Nørremark ◽  
Heta Mattila ◽  
Mika Keränen ◽  
Marja Hakala-Yatkin ◽  
...  

1985 ◽  
Vol 104 (3) ◽  
pp. 501-504 ◽  
Author(s):  
M. Havaux ◽  
R. Lannoye

SummaryDisks of hard wheat (Triticum durum Desf.) leaves subjected to rapid desiccation over 4 h showed noticeable changes in the shape of the in vivo chlorophyll fluorescence induction curves. In drought-sensitive varieties (such as Claridoc), water stress resulted in a strong inhibition of the slow fluorescence induction transients. In particular, the fluorescence quenching rate was markedly decreased in water-stressed leaf disks. In contrast, leaves of drought-resistant varieties (such as Aouedj) showed only minor changes in chlorophyll fluorescence. The results of this investigation suggest that the slow transient of the in vivo chlorophyll fluorescence induction phenomenon may provide a simple method for selecting drought-tolerant wheats.


1993 ◽  
Vol 48 (3-4) ◽  
pp. 379-384 ◽  
Author(s):  
Sangho Kim ◽  
Kriton K. Hatzios

The soybean cultivars “ Kwangkyo” and “ Hood ” are differentially sensitive to the bipyridylium herbicide paraquat (1,1′-dimethyl-4 ,4′-bipyridiniumion). This was confirmed by visible injury observations, measurements of desiccation levels and chlorophyll content, and tracings of chlorophyll fluorescence induction of fully expanded first trifoliate leaves of these two cultivars after exposure to a wide range of paraquat concentrations. The margin of this intraspecific differential tolerance to paraquat was narrow an d the ratio of the paraquat concentrations causing 50% injury to the tolerant Kwangkyo and to the susceptible Hood soybean (approximate tolerance factor) was found to be 10. Paraquat at 1 μᴍ or higher inhibited rapidly the CO2 fixation capacity of leaf mesophyll cells, isolated enzymatically from both cultivars. Thus, the tolerance of Kwangkyo soybean to paraquat does not appear to result from any differences at the site of paraquat action in chloroplast membranes. At early time periods (30 min to 2 h) after treatment with 100 μᴍ of paraquat, chlorophyll fluorescence induction was completely suppressed in first trifoliate leaves of Hood, but not in those of Kwangkyo soybean. At longer time periods (≧ 3 h), paraquat suppressed chlorophyll fluorescence induction similarly in leaves of both soybean cultivars. These results suggest that reduced mobility or a delayed release of paraquat in the mesophyll cells of Kwangkyo may be involved in the observed tolerance of this soybean cultivar to this herbicide.


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