scholarly journals Propagation of Lily Arumsari using Generic Media through In Vitro Culture

2020 ◽  
Vol 11 (2) ◽  
pp. 140-148
Author(s):  
Ridho Kurniati ◽  
Fauziah Khairatunnisa ◽  
Reni Indrayanti
Keyword(s):  
Plant Growth Regulator ◽  
Low Cost ◽  
Ms Medium ◽  
Single Factor ◽  
Mass Propagation ◽  
Murashige And Skoog Medium ◽  
Number Of Leaves ◽  
Leaves And Roots ◽  
The Cost

Lily was usually propagated using MS (Murashige and Skoog) medium supplemented with plant growth regulator and hormone. The important concerns in mass propagation and seed production of lily for industries are cheap, easy and low cost medium. This study’s objective was to find out of generic media to substitute MS medium and to decrease the cost of mass propagation of lily in vitro. Three substitutions medium were Vitagrow, Growmore, Gibril, and G-60. Bulbs of lily Arumsari varieties were used as materials. Complete Random Design with a single factor was used in the experimental design. The treatments were type of generic in vitro medium (Vitagrow, Growmore, Gibril, and G-60), consisting of three replication and 20 units per repetition and five bulbs per unit of repetition. The parameter observed was the total number of leaves and roots, length of leaves and roots, and bulb growth percentage. Growmore medium showed a better result than others in total number of leaves (50,67), length of leaves (1,6 cm), length of roots (0,312 cm) and percentage of bulb growth (100%). The highest total number of roots was achieved in G-60 medium.

scholarly journals In vitro clonal propagation and efficient acclimatization with use of hydrogel of intensively sweet medicinal plant Lippia dulcis Trev.

2020 ◽  
Vol 66 (4) ◽  
pp. 25-31
Author(s):  
Magdalena Tomaszewska-Sowa
Keyword(s):  
Leaf Area ◽  
Growth Regulators ◽  
Clonal Propagation ◽  
Shoot Length ◽  
Ms Medium ◽  
Ex Vitro ◽  
Mass Propagation ◽  
Number Of Leaves ◽  
In Vitro Clonal Propagation

Summary Introduction: The leaves of Lippia dulcis contain high amounts of hernandulcin. It is one thousand fold sweeter than sucrose, however, it hardly contains any calories. Objective: The aim of this research was to optimalisation of micropropagation and acclimatization of L dulcis Methods: The nodal explants were inoculated on phytohormone-free MS medium. After 6 weeks the explants were inoculated onto the MS medium with different plant growth regulators. Well-developed rooted plantlets were adapted to ex vitro conditions using hydrogel. Results: On the medium with BAP and NAA the highest number of shoots were produced. The higest average shoot length, number of the leaves and the leaf area were recorded on the medium with GA3. Adding IBA increased the number of roots. The addition of hydrogel enhanced the acclimatization efficiency. Conclusions: There was observed a positive, stimulating influence of growth regulators on mass propagation and increase in the number of leaves and the leaf area and influence of hydrogel on the development of plantlets during acclimatization.

scholarly journals Effects of Growth Regulators and Type-Variety of Oil Palm (Elaeis guineensis Jacq.) on Direct Organogenesis

2018 ◽  
Vol 10 (2) ◽  
pp. 251-258
Author(s):  
Chibuzo Nwabueze Chikezie NWAOGUALA ◽  
Hakeem Olalekan SHITTU
Keyword(s):  
Growth Regulators ◽  
Oil Palm ◽  
Root Length ◽  
Elaeis Guineensis ◽  
Root Production ◽  
Ms Medium ◽  
Number Of Leaves ◽  
Leaves And Roots ◽  
Elaeis Guineensis Jacq

The in vitro organogenesis of embryos of type-variety (‘Albescens’, ‘Nigrescens’, ‘Virescens’ and ‘Idolatrica’) of the oil palm (Elaeis guineensis Jacq.) with growth regulators was studied. Murashige and Skoog (MS) medium, supplemented with nine treatment concentrations of Naphthalene acetic acid (NAA) and 6-Benzylaminopurine (BAP), alone and in combinations, was used to initiate embryo cultures of the type-variety. Data were collected on time of root production, root length, plant height, number of leaves and number of roots.  The results obtained indicated that there were swelling and expansion of the matured zygotic explants in cultures within 7 and 14 days after culture initiation (DCI). Time of root production did not vary significantly among type-variety, but differed significantly (p < 0.001) due to growth regulators, whereas their interaction effect was non-significant. Root length and plantlet height significantly varied (p < 0.05) due to type-variety and growth regulators, as well as their interaction. There were no significant variations among type-variety on number of leaves and roots produced at 84 DCI, but there was significant (p < 0.05) variations on number of leaves and non-significant variance on number of roots due to growth regulators. The type-variety combined with growth regulators effects on number of leaves and roots were not significant.  In the present study, the responses of the various type-variety in the regeneration of plantlets in vitro varied with respect to media supplementation and that low concentration of NAA and BAP, alone and in different combinations, favoured root, leaf and plantlet production in MS medium.

scholarly journals STUDI PENGARUH MEDIA ALTERNATIF UNTUK PERBANYAKAN PISANG BARANGAN (Musa acuminata L.) SECARA IN-VITRO

2021 ◽  
Vol 12 (1) ◽  
pp. 33
Author(s):  
Rosmaina Rosmaina ◽  
Ragil Endika ◽  
Zulfahmi Zulfahmi
Keyword(s):  
Tissue Culture ◽  
Culture Media ◽  
Low Cost ◽  
Ms Medium ◽  
Liquid Fertilizer ◽  
Culture Techniques ◽  
Randomized Design ◽  
Media Component ◽  
The Cost

The main obstacles in the commercialization of seedlings mass propagation through tissue culture techniques is the high cost of the culture media component. therefore, the production of lowcost tissue culture is required. This study aims to develop low cost in-vitro media for the production of the seedlings of Barangan banana. The research was composed following Factorial Completely Randomized Design, the first factor was Terra Novalgro liquid fertilizer with three concentrations, namely 1, 2, and 3 ml L-1, while the second factor was Gandasil with 3 concentrations namely 1, 2, and 3 mg L-1, so obtained nine treatments, each treatment was repeated 10 times, so that there were 90 experimental units. MS media was used as control. The parameter observed was number of shoots, number of leaves and number of roots. The results of this study exhibited that the treatment of 1 ml L-1 liquid fertilizer + 2 mg L-1 foliar fertilizer produced 9.30 shoots/explant and 1.90 leaves/explant, that no significantly different from MS medium (control) which produced 9.0 shoots/explant and 0.3 leaves/explants. Therefore, the using completed liquid fertilizers and foliar fertilizers as medium in vitro propagation of barangan bananas can become an alternative replace MS medium, as well as reduced the cost of culture media as 91% -93% compared to MS media.

scholarly journals Effect of Explants and Low Cost Medium on Morphogenic Response of Aerial Stem and Rhizome Bud Explants of Turmeric (Curcuma longa L.) for Plant Regeneration

2020 ◽  
Vol 11 ◽  
pp. 15-24
Author(s):  
Hewa Fonsekage Lakma Upendri ◽  
Thayamini H. Seran
Keyword(s):  
Low Cost ◽  
Stem Explants ◽  
Ms Medium ◽  
Shoot Bud ◽  
Rhizome Bud ◽  
Aerial Stem ◽  
The Cost ◽  
Bud Initiation ◽  
Embryogenic Response

This study was done to select suitable explants and low cost medium for plant regeneration of turmeric. Therefore, the different explants were excised from the aerial stems and rhizome buds and surface sterilized. The sterilized explants were cultured on MS medium fortified with 2.0 mg/l BAP. From the survived aerial stem explants, 0.5 cm long vertical half of the aerial stem explants exhibited somatic embryogenic response (69.7%). The highest morphogenic response (74%) of shoot bud initiation was observed from the top slice of the surviving rhizome bud explants. Further, Yara Mila complex fertilizer, which is an ideal granular fertilizer mixture, was used as an alternative to MS medium. Three different concentrations of Yara Mila complex fertilizer (1.0, 3.0, and 5.0 g/l ) supplemented with 2.0 mg/l BAP each were tested with the MS medium fortified with 2.0 mg/l BAP (control treatment) for in vitro establishment from aerial stem explants and top slice of the rhizome bud explants. Both explants were surface sterilized and cultured on MS medium and different concentrations of Yara Mila complex fertilizer fortified with 2.0 mg/l BAP. From the survived explants, aerial stem explants exhibited somatic embryogenic response (69.7%) and shoot bud initiation (74%) on normal MS media. The higher performances were observed in 1.0 g/l concentration of complex fertilizer incorporated medium with 51% embryogenic response from the aerial stem explants and 52.3% shoot bud formation response from the top slice of the rhizome bud. The cost of 1 kg complex fertilizer was Rs. 182. It could be concluded that complex fertilizer is a cost effective alternative medium for MS medium for in vitro propagation reducing the cost of the substituted ingredients by 99.87% in the tissue culture of turmeric.

scholarly journals GROWTH OF ‘PRATA-ANA’ BANANA’S MICROSHOOTS CLONE GORUTUBA FROM SYNTHETIC SEEDS:SUBSTRATES AND BAP CONCENTRATION

2017 ◽  
Vol 39 (5) ◽  
Author(s):  
JÚLIO CÉSAR GOMES PEREIRA ◽  
SELMA SILVA ROCHA ◽  
LUCIANA CARDOSO NOGUEIRA LONDE ◽  
MARCELA CAROLINE BATISTA DA MOTA ◽  
PABLO FERNANDO SANTOS ALVES ◽  
...  
Keyword(s):  
Culture Medium ◽  
Economic Importance ◽  
Leaf Length ◽  
Naphthalene Acetic Acid ◽  
Synthetic Seed ◽  
Ms Medium ◽  
World Production ◽  
Number Of Leaves ◽  
Alginate Matrix

ABSTRACT The banana crop stands out as an activity of great social and economic importance in Brazil, which occupies the fifth place in world production. Synthetic seed production is becoming promising for a micropropagation and in vitro conservation. The aim of the study was to analyze the conversion and growth of ‘Prata-anã’ banana’s microshoots clone Gorutuba from synthetic seed in MS medium and vermiculite, different substrates and concentrations of BAP (6-benzylaminopurine) associated with ANA (acetic naphthalene acid) in the constitution of its capsule were tested. The microshoots were immersed in the sodium alginate matrix (3%) and dripped in a solution of CaCl2.2H2O (100 mM) for complexation and then in KNO3 solution (100 mM) to decomplex. The experimental design was completely randomized in a 2 x 5 factorial design (substrate x BAP concentrations), containing different substrates (MS culture medium and vermiculite) and BAP concentrations (2.22, 4.44, 6.66, 8.88 and 13.32 µmol L-1) associated with NAA (naphthalene acetic acid) 0.54 µmol L-1, totaling 10 treatments, with 4 replicates, and that each replicate containing 5 seeds. The evaluations of conversion, number of leaves, leaf length, leaf height, number of roots, root length and oxidation were performed at 30 and 60 days.The use of the MS medium provided better growth results in relation to vermiculite as substrate, in which the different BAP concentrations did not differ from each other. It was found that, in MS culture medium, BAP concentrations above 8.88 µmol L-1 in the capsule composition are not indicated for microshoots growth.

scholarly journals EFFECT OF DIFFERENT SOURCES OF PLANT GROWTH REGULATOR ON THE INDUCTION AND DEVELOPMENT OF MANGOSTEEN SOMATIC EMBRYOS

2017 ◽  
Vol 17 (1) ◽  
pp. 9
Author(s):  
Yosi Zendra Joni ◽  
Riry Prihatini ◽  
Darda Efendi ◽  
Ika Roostika
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Growth ◽  
Embryogenic Callus ◽  
Plant Growth Regulator ◽  
Somatic Embryos ◽  
Casein Hydrolysate ◽  
Malt Extract ◽  
Mass Propagation ◽  
Embryogenic Callus Induction

<p>Somatic embryogenesis is a technique for regenerating embryos derived from somatic cells of various plant species. This technique along with the utilization of plant growth regulator (PGR) might benefit for mass propagation and improvement of plant species through biotechnological tools. The study aimed to determine the effect of different plant growth regu-lators, namely 6-benzyladenine (BA) and thidiazuron (TDZ) on the embryogenic callus induction as well as casein hydrolysate and malt extract on the somatic embryo development of mangosteen. The explants used were in vitro young stems of mangosteen clone Leuwiliang. This study consisted of two experiments, namely induction of embryogenic callus and formation of somatic embryo. The first experiment was arranged as factorial in a completely randomized design with BA (0 and 0.7 mg l-1) as the first factor and TDZ (0, 0.1, 0.5 and 1.0 mg l-1) as the second factor. The second experiment consisted of four treatments, i.e. casein hydrolysate and malt extract at the rate of 500 and 1,000 mg l-1. The results showed that the best medium for embryogenic callus induction was MS supplemented with 0.1 mg l-1 TDZ, which resulted semifriable calli. Casein hydrolysate and malt extract could not induce the formation of somatic embryos. After two times subcultures on the same MS medium supplemented with 0.5 mg l-1 TDZ and 0.7 mg l-1 BA, a total of 33.8 somatic embryos per explant was induced. The successful somatic embryogenesis would support mangosteen breeding and in vitro mass propagation program.</p>

scholarly journals Impact of saline solution on growth and photosystem II during in vitro cultivation of Bromelia antiacantha (Bromeliaceae)

Rodriguésia ◽  
2021 ◽  
Vol 72 ◽  
Author(s):  
Rosiane Cipriano ◽  
João Paulo Rodrigues Martins ◽  
Luiz Carlos de Almeida Rodrigues ◽  
Antelmo Ralph Falqueto ◽  
Andreia Barcelos Passos Lima Gontijo
Keyword(s):  
Photosystem Ii ◽  
Saline Solution ◽  
Growth Traits ◽  
Photosynthetic Apparatus ◽  
Solution Culture ◽  
In Vitro Cultivation ◽  
Ms Medium ◽  
Number Of Leaves ◽  
Positive Effect

Abstract In vitro cultivation is a technique with wide application for micropropagation. However, each species has specific mineral needs for this type of cultivation. The objective was to assess the impacts of the saline solution culture medium on the performance of the photosynthetic apparatus and growth of Bromelia antiacantha during in vitro cultivation, and thus to elucidate the mitigation of the nutritional imbalance that can interfere in the electron transport in the plants. Plants were cultivated in a salt concentration gradient of MS medium (0%, 25%, 50%, 75% or 100%). The growth traits and fluorescence a chlorophyll were analyzed. Intermediate concentrations of MS medium resulted in plants with a larger number of leaves and longer root length. The OJIP curves and results of the JIP test showed that the plants grown without MS salts presented less efficient photosystem II (PSII), as indicated by the performance index [Pi(total)]. In contrast, the intermediate concentrations (MS 25% and 50%) had a positive effect on the performance of the photosynthetic apparatus. The MS 25% medium can be used for in vitro cultivation of B. antiacantha, enabling the development of plants with suitable physiological qualities for planting in the field.

scholarly journals Mass Propagation of Nauclea diderrichii (de Willd. & Th. Dur) Merr. Seedlings through Tissue Culture Technique

2021 ◽  
Vol 31 (1) ◽  
pp. 51-60
Author(s):  
RI Oyediran ◽  
JO Afolabi ◽  
DB Olomola ◽  
FO Akanni
Keyword(s):  
Tissue Culture ◽  
Adventitious Shoots ◽  
Basal Medium ◽  
Culture Technique ◽  
Seedling Production ◽  
Mass Propagation ◽  
In Vitro Plantlets ◽  
Number Of Leaves ◽  
Nauclea Diderrichii

Nauclea diderrichii is a tree species of economic importance. However, its plantation establishment is limited by inadequate seedling production. Hence, there is ample scope of tissue culture for its mass propagation. Its in vitro plantlets development as affected by media strengths indicated that 100 % seed germination was obtained in full MS basal medium while the least (3.35 %) was from quarter-strength at 8 Weeks after inoculation (WAI). The effects of BAP and NAA assessed on the growth of its sub-cultured plantlets showed that highest number of leaves (17) and adventitious shoots (3) were obtained from MS basal medium supplemented with 0.1 mg/l BAP only. Whereas, highest shoot length (3.61 cm) and average number of roots (5/plantlet) were obtained from the same medium without hormone(s) at 8 WAI. Further sub-culturing into MS with 0.05 mg/l NAA resulted into plantlets having optimum shoot and massive root growth ready for acclimatization in 6 WAI. The plantlets were successfully acclimatized using coconuthusk/ topsoil mixture with 90 % survival. Plant Tissue Cult. & Biotech. 31(1): 51-60, 2021 (June)

scholarly journals Mass Propagation of Feronia limonia L. through Tissue Culture

1970 ◽  
Vol 45 (1) ◽  
pp. 75-78 ◽  
Author(s):  
Shahina Islam ◽  
Mosfequa Zahan ◽  
Shahina Akter ◽  
Tanjina Akhtar Banu ◽  
Ahashan Habib ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Key Words ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Nodal Explants ◽  
Ms Medium ◽  
Ex Vitro ◽  
Mass Propagation

An efficient mass propagation method for Feronia limonia was developed from excised shoot tips and nodal explants of in vitro grown seedlings. Explants were cultured on MS medium with different conc. of NAA, Kn, IAA and BAP singly or in combinations. Highest number of micro shoots and better plant growth were obtained from these two explants on MS medium supplemented with 0.2 mg/l BAP alone. The regenerated shoots were successfully rooted on MS medium supplemented with 0.5 mg/l NAA. The in vitro raised plantlets were successfully established in soil following the formation of roots with 100% survivability under ex vitro condition. Key words: Feronia limonia; Mass propagation; Node; Shoot tips; Multiple shoot DOI: 10.3329/bjsir.v45i1.5186 Bangladesh J. Sci. Ind. Res. 45(1), 75-78, 2010

scholarly journals In vitro growth and development of Dendrobium hybrid orchid

1970 ◽  
Vol 35 (3) ◽  
pp. 507-514 ◽  
Author(s):  
H Khatun ◽  
MM Khatun ◽  
MS Biswas ◽  
MR Kabir ◽  
M Al-Amin
Keyword(s):  
Growth Regulators ◽  
Growth And Development ◽  
Ms Medium ◽  
Fresh Weight ◽  
Maximum Weight ◽  
Shoot Height ◽  
Single Shoot ◽  
Number Of Leaves ◽  
Regenerated Plantlets

The experiment was conducted to investigate the combined effect of different plant growth regulators and charcoal supplementation in MS medium on growth and development of plantlets regenerated from protocorm like bodies (PLBs) of hybrid orchid. The combination of BAP + NAA, BAP + IAA, BAP + IBA, and IAA + IBA at different concentrations with charcoal supplementation was studied. The result revealed that the use of different growth regulators had significant effect on different parameters studied. The maximum weight of PLBs (5.123 g) was obtained from the combination of BAP + IBA at 1.0 mg/l each. The highest shoot height (3.239 cm) and maximum number of rooted plantlets (4.473) was obtained from 1.0 mg/l each of BAP + NAA combination. The maximum number of leaves (3.490) and the maximum length of leaves (1.946 cm) were obtained from 1.0 mg/l each of BAP + IBA and the highest leaf width (1.166 cm) was obtained from 0.5 mg/l BAP +1.0 mg/l IBA combination. The highest root length was obtained from 0.5 mg/l each of BAP + IAA and the maximum number of regenerated plantlets (20) was obtained from 0.5 mg/l IAA + 1.0 mg/l IBA combination. However, the maximum fresh weight of single shoot (0.219 g) and the maximum number of roots per plantlet (6.300) was obtained from 1.0 mg/I each of IAA + IBA combination. Keywords: Dedrobium; orchid; hybrid; In vitro growth. DOI: 10.3329/bjar.v35i3.6457Bangladesh J. Agril. Res. 35(3) : 507-514

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