Wave propagation for a reduced model of blood coagulation

Reduced mathematical models of blood coagulation can properly describe Thrombin Generation Test (TGT). Clot growth occurs as a reactiondiffusion wave, and the reduced model studied in this work describes this behaviour, preserving the differences observed between healthy and hemophilia subjects in the TGT.

Acute Pancreatitis in Man and Blood Coagulation Disturbances

10.1055/s-0039-1687504 ◽

1979 ◽

Author(s):

H. Kõtering ◽

M. Hasenbein ◽

H. Artmann ◽

U. Kasten ◽

J. Kellermann

Keyword(s):

Acute Pancreatitis ◽

Blood Coagulation ◽

Thrombin Generation ◽

The Other ◽

Phospholipase A ◽

Coagulation System ◽

Consumption Coagulopathy ◽

Blood Coagulation System ◽

Severe Pancreatitis ◽

The pathogenesis of blood coagulation-disturbances in patients with acute pancreatitis in man is still unknown. Therefore we studied repeatedly the blood coagulation system of all patients with acute pancreatitis, who were admitted to our clinic or were transferred from other hospitals after complications occurred. 19 patients with a severe pancreatitia were studied. Most of them showed oliguria, pancreatic lungs, thrombosis or haemorrhage. Only 9 determinations (in 5 patients) resulted an enhancement of thrombin generation in the Thrombin-Generation-Test (TGT). All the other patients showed already hypocoagulsbility in the TGT and severe signs of DIC and consumption coagulopathy with a loss of platelets, fibrinogen and prothrombin complex. In 9 patients, who died, we found histomorphologicaliy fibrin deposites and hyaline thrombi. In comparison to 58 patients with elevated amylases but no severe pancreatitiS we found, that the initial alteration of blood coagulation system in pancreatitis is a hypercoagulaoility, possibly caused by trypsin, phospholipase A or elastase.

Coagulation status in patients with pulmonary embolism receiving long-term anticoagulant therapy

CARDIOVASCULAR THERAPY AND PREVENTION ◽

10.15829/1728-8800-2021-3093 ◽

2022 ◽

Vol 20 (8) ◽

pp. 3093

Author(s):

E. A. Shmidt ◽

S. A. Berns ◽

T. Yu. Penskaya ◽

I. I. Zhidkova ◽

O. V. Gruzdeva ◽

...

Keyword(s):

Pulmonary Embolism ◽

Blood Coagulation ◽

Anticoagulant Therapy ◽

Thrombin Generation ◽

Fibrin Clot ◽

Coagulation Rate ◽

Integral Methods ◽

Coagulation Status ◽

Aim. To study the blood coagulation status by various laboratory methods in patients after pulmonary embolism (PE) receiving long-term anticoagulant therapy.Material and methods. The blood of 23 patients with pulmonary embolism, who received long-term anticoagulant therapy, was studied. The study of coagulation profile, D-dimer, thrombodynamics, thromboelastography and thrombin generation test were carried out.Results. The thrombin generation test shows a significant increase in the time of its formation, while the maximum amount of thrombin formed is half that of the reference values. There is a slightly increased median fibrin clot growth rate in the thrombodynamics test — 30,4 gm/min with a normal coagulation rate of 20-29 gm/min. The result of thromboelastography also reflects the blood hypocoagulation, in terms of R, Angle a and CI.Conclusion. Integral methods for assessing the thrombotic readiness in combination with a routine coagulation panel demonstrate a complete picture of blood coagulation potential in patients after pulmonary embolism requiring long-term anticoagulant therapy.

The Effect of Trasylol on Blood Coagulation after Intravenous Injection

10.1055/s-0038-1654110 ◽

1967 ◽

Vol 17 (01/02) ◽

pp. 051-057

Author(s):

J Gormsen ◽

P Josephsen

Keyword(s):

Blood Coagulation ◽

Intravenous Injection ◽

Significant Influence ◽

Partial Thromboplastin Time ◽

Thrombin Generation ◽

Double Blind ◽

SummaryThe in vivo effect of Trasylol® on the thromboplastin generation test, thrombin -generation test, the partial thromboplastin time and the thrombelastography has been examined in 50 patients. Furthermore 34 patients were given one of two preparations of “Trasylol”, distributed for a double-blind control. The dosis of Trasylol varied from 100,000 to 400,000 units. No significant influence on the various tests used was found.

Is There an Enhancing Effect of Neuroleptic Drugs on Blood Coagulation and Thrombosis

10.1055/s-0038-1656241 ◽

1965 ◽

Vol 13 (02) ◽

pp. 418-427 ◽

Cited By ~ 2

Author(s):

T Astrup ◽

J Rasmussen

Keyword(s):

Blood Coagulation ◽

Direct Effect ◽

Thrombin Generation ◽

Neuroleptic Drugs ◽

Secondary Effects ◽

Heavy Sedation ◽

Inhibiting Effect ◽

Normal Human ◽

Significant Direct Effect ◽

SummarySordinol (R), (clopenthixol, W.H. 0.), a potent sedative of the thiaxanthene group, in the concentrations used therapeutically, had no direct influence on blood coagulation as investigated by the thrombin generation test and the thromboplastin activation test. No significant direct effect on the fibrinolytic system was observed using urokinase, streptokinase activated human euglobulin, trypsin, porcine plasmin, or human plasmin. There was no effect on the urokinase-inhibiting effect of normal human plasma. The influence of the immobilization produced by heavy sedation on the development of thrombotic states is discussed. When the effects of drugs are evaluated it is necessary to distinguish between the direct effect of the drug and secondary effects caused by the treatment.

The Effect of Fatty-acid Autoxidation Products on Blood Coagulation

10.1055/s-0038-1647880 ◽

1975 ◽

Vol 33 (02) ◽

pp. 271-277 ◽

Cited By ~ 12

Author(s):

T. W Barrowcliffe ◽

J. M. C Guttteridge ◽

T. L Dormandy

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Polyunsaturated Fatty Acids ◽

Blood Coagulation ◽

Thrombin Generation ◽

Water Soluble ◽

Oxidation Products ◽

Coagulant Activity ◽

Rate Of Decay ◽

SummaryPolyunsaturated fatty acids were allowed to autoxidise in air over 4 days. The water soluble oxidation products were extracted at daily intervals and tested for their effect on blood coagulation. After 1 day there was slight acceleration of the recalcification and RVV times, but from 2–4 days the extracts became increasingly inhibitory. The P.T. and P.T.T. were also inhibited. In the thrombin generation test the extracts delayed the appearance of thrombin, but the peak thrombin level was increased and its rate of decay was reduced. When added to phospholipid the extracts altered their coagulant activity. The presence of autoxidation products could account for some of the variable results obtained with different preparations of phospholipids.

Clustering of Thrombin Generation Test Data Using a Reduced Mathematical Model of Blood Coagulation

Acta Biotheoretica ◽

10.1007/s10441-019-09372-w ◽

2019 ◽

Vol 68 (1) ◽

pp. 21-43 ◽

Cited By ~ 1

Author(s):

N. Ratto ◽

A. Tokarev ◽

P. Chelle ◽

B. Tardy-Poncet ◽

V. Volpert

Keyword(s):

Mathematical Model ◽

Blood Coagulation ◽

Test Data ◽

Thrombin Generation ◽

Experimental Studies on a Recombinant Hirudin, CGP 39393

10.1055/s-0038-1648151 ◽

1991 ◽

Vol 65 (04) ◽

pp. 355-359 ◽

Cited By ~ 12

Author(s):

E Gray ◽

J Watton ◽

S Cesmeli ◽

T W Barrowcliffe ◽

D P Thomas

Keyword(s):

Thrombin Generation ◽

Bleeding Time ◽

Thrombus Formation ◽

Experimental Studies ◽

Venous Stasis ◽

Antithrombotic Agent ◽

Recombinant Hirudin ◽

Excessive Bleeding ◽

SummaryThe in vitro anticoagulant activities of recombinant desulphatohirudin (r-hirudin) were studied in the activated partial thromboplastin time (APTT) and the thrombin generation test : systems. In the APTT at concentrations below 5 μg/ml, r-hirudin showed a dose-response curye. At concentrations above 5 μg/ml, the plasma became unclottable, but in the thrombin generation test , at least 10 μg/ml of r-hirudin was required for full inhibition of thrombin generation. The antithrombotic effect was assessed using a rabbit venous stasis model; 150 μg/ml r-hirudin completely prevented thrombus formation at 10 and 20 min stasis. At antithrombotic dose, the mean bleeding time ratio measured in a rabbit ear template model, was not prolonged over control values. At higher doses, the bleeding time ratios were higher than those observed for the same dosage of heparin. These data indicate that while r-hirudin is an effective antithrombotic agent, antithrombotic doses have to be carefully titrated to avoid excessive bleeding.

The Ability of Thrombin Inhibitors to Reduce the Thrombin Activity Generated in Plasma on Extrinsic and Intrinsic Activation

10.1055/s-0038-1655996 ◽

1997 ◽

Vol 77 (03) ◽

pp. 498-503 ◽

Cited By ~ 26

Author(s):

D Prasa ◽

L Svendsen ◽

J Stürzebecher

Keyword(s):

Active Site ◽

Thrombin Generation ◽

Anion Binding ◽

Thrombin Inhibitors ◽

Coagulation System ◽

Thrombin Activity ◽

Continuous Registration ◽

High Concentrations ◽

20 Nm ◽

SummaryIn a thrombin generation test with continuous registration of thrombin activity in plasma we studied the ability of a variety of thrombin inhibitors of different type and mechanism of action to influence the activity of thrombin after activation of the coagulation system. Depending on the inhibitor, the peak of thrombin activity is delayed and/or reduced.By blocking the active site of generated thrombin inhibitors cause a concentration dependent reduction of the thrombin peak and inhibit feed-back reactions of thrombin resulting in a delay of thrombin generation. Highly potent synthetic active-site directed inhibitors (Ki ≤ 20 nM) reduce the thrombin activity formed in plasma after extrinsic or intrinsic activation with the same efficiency (IC50 0.1 - 0.6 μM) as hirudin. The delay and reduction of thrombin generation by inhibitors of the anion-binding exosite 1 of thrombin is only attributed to an inhibition of feed-back reactions of thrombin. For a 50% reduction of thrombin activity in plasma by this type of inhibitors relatively high concentrations were determined.