Nerve Regeneration Enhancement by Tourniquet

2001 ◽  
Vol 26 (4) ◽  
pp. 347-351 ◽  
Author(s):  
A. WIDERBERG ◽  
G. LUNDBORG ◽  
L. B. DAHLIN
Keyword(s):  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Axonal Regeneration ◽  
Hind Limb ◽  
Lesion Effect ◽  
Non Invasive ◽  
Rat Sciatic Nerve ◽  
Sensory Axons ◽  
Invasive Method

The use of tourniquet compression as a non-invasive method to enhance axonal regeneration was assessed in the rat sciatic nerve. One hind limb of the rat was subjected to compression by a tourniquet set at 300 mmHg for 30 or 120 min followed by bilateral test crush lesions performed either directly or after a conditioning interval of 3 or 6 days, with the non-compressed side serving as a control. Axonal regeneration distances were evaluated after 3 days by the pinch reflex test. We found that compression caused an increased outgrowth length of sensory axons compared to the controls. The effect was most obvious after 120 min of compression with a conditioning interval of 6 days. Tourniquet compression has a conditioning lesion effect on peripheral nerve and may enhance nerve regeneration.

scholarly journals Gellan Gum-based luminal fillers for peripheral nerve regeneration: an in vivo study in the rat sciatic nerve repair model

2018 ◽  
Vol 6 (5) ◽  
pp. 1059-1075 ◽  
Author(s):  
C. R. Carvalho ◽  
S. Wrobel ◽  
C. Meyer ◽  
C. Brandenberger ◽  
I. F. Cengiz ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Experimental Work ◽  
Nerve Repair ◽  
Peripheral Nerve Regeneration ◽  
Gellan Gum ◽  
In Vivo Study ◽  
Rat Sciatic Nerve

This experimental work considers the innovative use of the biomaterial Gellan Gum (GG) as a luminal filler for nerve guidance channels.

scholarly journals The efficacy of a scaffold-free Bio 3D conduit developed from human fibroblasts on peripheral nerve regeneration in a rat sciatic nerve model

PLoS ONE ◽  
2017 ◽  
Vol 12 (2) ◽  
pp. e0171448 ◽  
Author(s):  
Hirofumi Yurie ◽  
Ryosuke Ikeguchi ◽  
Tomoki Aoyama ◽  
Yukitoshi Kaizawa ◽  
Junichi Tajino ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Peripheral Nerve Regeneration ◽  
Human Fibroblasts ◽  
Rat Sciatic Nerve ◽  
Nerve Model

scholarly journals Correlation between parameters of electrophysiological, histomorphometric and sciatic functional index evaluations after rat sciatic nerve repair

2006 ◽  
Vol 64 (3b) ◽  
pp. 750-756 ◽  
Author(s):  
Roberto Sergio Martins ◽  
Mario Gilberto Siqueira ◽  
Ciro Ferreira da Silva ◽  
José Píndaro Pereira Plese
Keyword(s):  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Fiber Diameter ◽  
Nerve Repair ◽  
Neural Regeneration ◽  
Poor Correlation ◽  
Functional Index ◽  
Rat Sciatic Nerve ◽  
Sciatic Functional Index

The rat sciatic nerve is a well-established model for the study of recovery from peripheral nerve injuries. Traditional methods of assessing nerve regeneration after nerve injury and repair, such as electrophysiology and histomorphometry, despite widely used in neural regeneration experiments, do not necessarily correlate with return of motor and sensory functions. The aim of this experimental study is to investigate the possible correlation between several parameters of peripheral nerve regeneration after repair of sectioned sciatic nerve in Wistar rat. A two-stage approach was used to obtain 17 parameters after electrophysiological, morphometric and sciatic functional index evaluations. Pearson's correlation analysis was performed between these results. Only two positives correlations between different classes of peripheral nerve assessments were noted, between sciatic functional index and proximal nerve fiber diameter (r=0.56, p<0.01) and between sciatic functional index and distal fiber diameter (r=0.50, p<0.01). The data presented in our study demonstrates that there is a poor correlation between the sciatic functional index and outcome measures of electrophysiological and morphometric evaluations.

scholarly journals Biomimetic nerve guidance conduit containing engineered exosomes of adipose-derived stem cells promotes peripheral nerve regeneration

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Zheng Yang ◽  
Yang Yang ◽  
Yichi Xu ◽  
Weiqian Jiang ◽  
Yan Shao ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Peripheral Nerve Regeneration ◽  
Function Evaluation ◽  
Functional Protein ◽  
Rat Sciatic Nerve ◽  
Nerve Guidance Conduit ◽  
Gastrocnemius Muscles

Abstract Background Efficient and stable delivery of neurotrophic factors (NTFs) is crucial to provide suitable microenvironment for peripheral nerve regeneration. Neurotrophin-3 (NT-3) is an important NTF during peripheral nerve regeneration which is scarce in the first few weeks of nerve defect. Exosomes are nanovesicles and have been served as promising candidate for biocarrier. In this work, NT-3 mRNA was encapsulated in adipose-derived stem cell (ADSC)-derived exosomes (ExoNT-3). These engineered exosomes were applied as NT-3 mRNA carrier and then were loaded in nerve guidance conduit (ExoNT-3-NGC) to bridge rat sciatic nerve defect. Method NT-3 mRNA was encapsulated in exosomes by forcedly expression of NT-3 mRNA in the donor ADSCs. ExoNT-3 were co-cultured with SCs in vitro; after 24 h of culture, the efficiency of NT-3 mRNA delivery was evaluated by qPCR, western blotting and ELISA. Then, ExoNT-3 were loaded in alginate hydrogel to construct the nerve guidance conduits (ExoNT-3-NGC). ExoNT-3-NGC were implanted in vivo to reconstruct 10 mm rat sciatic nerve defect. The expression of NT-3 was measured 2 weeks after the implantation operation. The sciatic nerve functional index (SFI) was examined at 2 and 8 weeks after the operation. Moreover, the therapeutic effect of ExoNT-3-NGC was also evaluated by morphology assay, immunofluorescence staining of regenerated nerves, function evaluation of gastrocnemius muscles after 8 weeks of implantation. Results The engineered exosomes could deliver NT-3 mRNA to the recipient cells efficiently and translated into functional protein. The constructed NGC could realize stable release of exosomes at least for 2 weeks. After NGC implantation in vivo, ExoNT-3-NGC group significantly promote nerve regeneration and improve the function recovery of gastrocnemius muscles compared with control exosomes (Exoempty-NGC) group. Conclusion In this work, NGC was constructed to allow exosome-mediated NT-3 mRNA delivery. After ExoNT-3-NGC implantation in vivo, the level of NT-3 could restore which enhance the nerve regeneration. Our study provide a potential approach to improve nerve regeneration.

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