scholarly journals Correlation between parameters of electrophysiological, histomorphometric and sciatic functional index evaluations after rat sciatic nerve repair

2006 ◽  
Vol 64 (3b) ◽  
pp. 750-756 ◽  
Author(s):  
Roberto Sergio Martins ◽  
Mario Gilberto Siqueira ◽  
Ciro Ferreira da Silva ◽  
José Píndaro Pereira Plese
Keyword(s):  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Fiber Diameter ◽  
Nerve Repair ◽  
Neural Regeneration ◽  
Poor Correlation ◽  
Functional Index ◽  
Rat Sciatic Nerve ◽  
Sciatic Functional Index

The rat sciatic nerve is a well-established model for the study of recovery from peripheral nerve injuries. Traditional methods of assessing nerve regeneration after nerve injury and repair, such as electrophysiology and histomorphometry, despite widely used in neural regeneration experiments, do not necessarily correlate with return of motor and sensory functions. The aim of this experimental study is to investigate the possible correlation between several parameters of peripheral nerve regeneration after repair of sectioned sciatic nerve in Wistar rat. A two-stage approach was used to obtain 17 parameters after electrophysiological, morphometric and sciatic functional index evaluations. Pearson's correlation analysis was performed between these results. Only two positives correlations between different classes of peripheral nerve assessments were noted, between sciatic functional index and proximal nerve fiber diameter (r=0.56, p<0.01) and between sciatic functional index and distal fiber diameter (r=0.50, p<0.01). The data presented in our study demonstrates that there is a poor correlation between the sciatic functional index and outcome measures of electrophysiological and morphometric evaluations.

scholarly journals Gellan Gum-based luminal fillers for peripheral nerve regeneration: an in vivo study in the rat sciatic nerve repair model

2018 ◽  
Vol 6 (5) ◽  
pp. 1059-1075 ◽  
Author(s):  
C. R. Carvalho ◽  
S. Wrobel ◽  
C. Meyer ◽  
C. Brandenberger ◽  
I. F. Cengiz ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Experimental Work ◽  
Nerve Repair ◽  
Peripheral Nerve Regeneration ◽  
Gellan Gum ◽  
In Vivo Study ◽  
Rat Sciatic Nerve

This experimental work considers the innovative use of the biomaterial Gellan Gum (GG) as a luminal filler for nerve guidance channels.

scholarly journals IL-17F depletion accelerates chitosan conduit guided peripheral nerve regeneration

2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Feixiang Chen ◽  
Weihuang Liu ◽  
Qiang Zhang ◽  
Ping Wu ◽  
Ao Xiao ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Knockout Mice ◽  
Inflammatory Markers ◽  
Nerve Repair ◽  
Peripheral Nerve Regeneration ◽  
Wild Type ◽  
Anti Inflammatory

AbstractPeripheral nerve injury is a serious health problem and repairing long nerve deficits remains a clinical challenge nowadays. Nerve guidance conduit (NGC) serves as the most promising alternative therapy strategy to autografts but its repairing efficiency needs improvement. In this study, we investigated whether modulating the immune microenvironment by Interleukin-17F (IL-17F) could promote NGC mediated peripheral nerve repair. Chitosan conduits were used to bridge sciatic nerve defect in IL-17F knockout mice and wild-type mice with autografts as controls. Our data revealed that IL-17F knockout mice had improved functional recovery and axonal regeneration of sciatic nerve bridged by chitosan conduits comparing to the wild-type mice. Notably, IL-17F knockout mice had enhanced anti-inflammatory macrophages in the NGC repairing microenvironment. In vitro data revealed that IL-17F knockout peritoneal and bone marrow derived macrophages had increased anti-inflammatory markers after treatment with the extracts from chitosan conduits, while higher pro-inflammatory markers were detected in the Raw264.7 macrophage cell line, wild-type peritoneal and bone marrow derived macrophages after the same treatment. The biased anti-inflammatory phenotype of macrophages by IL-17F knockout probably contributed to the improved chitosan conduit guided sciatic nerve regeneration. Additionally, IL-17F could enhance pro-inflammatory factors production in Raw264.7 cells and wild-type peritoneal macrophages. Altogether, IL-17F may partially mediate chitosan conduit induced pro-inflammatory polarization of macrophages during nerve repair. These results not only revealed a role of IL-17F in macrophage function, but also provided a unique and promising target, IL-17F, to modulate the microenvironment and enhance the peripheral nerve regeneration.

scholarly journals Learning curve in rat dissection for experimental sciatic nerve repair

2019 ◽  
pp. 243-248
Author(s):  
Marin Andrei ◽  
Marin Georgiana Gabriela ◽  
Dobrete Nicoleta Amalia ◽  
Enescu Dan Mircea
Keyword(s):  
Sciatic Nerve ◽  
Learning Curve ◽  
Nerve Regeneration ◽  
Experimental Model ◽  
Rat Model ◽  
Surgical Intervention ◽  
Nerve Repair ◽  
Learning Curves ◽  
Rat Sciatic Nerve ◽  
Nerve Surgery

The baseline for any key research in nerve regeneration is an experimental model and the sciatic nerve in the rat model is the workhorse in this field. Although physically resistant to external traumas, a surgical intervention constitutes a major distress even for a rat. In the following presentation, we will analyse the learning curves for different stages in the rat sciatic nerve surgery as well as possible factors which influence these times.

scholarly journals Mesenchymal Stem Cells Enhance Nerve Regeneration in a Rat Sciatic Nerve Repair and Hindlimb Transplant Model

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Damon S. Cooney ◽  
Eric G. Wimmers ◽  
Zuhaib Ibrahim ◽  
Johanna Grahammer ◽  
Joani M. Christensen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Sciatic Nerve ◽  
Nerve Regeneration ◽  
Nerve Repair ◽  
Rat Sciatic Nerve ◽  
Transplant Model

scholarly journals The efficacy of a scaffold-free Bio 3D conduit developed from human fibroblasts on peripheral nerve regeneration in a rat sciatic nerve model

PLoS ONE ◽  
2017 ◽  
Vol 12 (2) ◽  
pp. e0171448 ◽  
Author(s):  
Hirofumi Yurie ◽  
Ryosuke Ikeguchi ◽  
Tomoki Aoyama ◽  
Yukitoshi Kaizawa ◽  
Junichi Tajino ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Peripheral Nerve Regeneration ◽  
Human Fibroblasts ◽  
Rat Sciatic Nerve ◽  
Nerve Model

Stimulated Jitter Measurement in the Assessment of Recovery after different Methods of Peripheral Nerve Repair

1998 ◽  
Vol 23 (1) ◽  
pp. 12-16 ◽  
Author(s):  
D. V. LENIHAN ◽  
N. M. SOJITRA ◽  
M. A. GLASBY
Keyword(s):  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Neuromuscular Junctions ◽  
Nerve Repair ◽  
Neuromuscular Function ◽  
Electrophysiological Recording ◽  
Peripheral Nerve Repair ◽  
Future Developments ◽  
Jitter Measurement

The recording of stimulated jitter offers a quantitative method for following the recovery of neuromuscular function after peripheral nerve repair. In groups of rats, electrophysiological recording of jitter was carried out on control animals and on animals 90 days after sciatic nerve division and subsequent repair with either direct end-to-end suture (NS), nerve graft (NG) or freeze thawed muscle graft (FTMG). It was found that values for jitter were highest in the FTMG group. The NS and NG groups demonstrated statistically similar jitter values when compared with each other and with the normal. It was concluded that the speed of nerve regeneration is slower in the FTMG group, at least initially, and that 90 days after sciatic nerve repair the FMTG group had an increase in the number of immature neuromuscular junctions when compared with the NS or NG groups. Jitter measurement would appear to offer a means of detecting small differences in nerve regeneration. The value of this in future developments in nerve repair is discussed.

scholarly journals EpimediumExtract Promotes Peripheral Nerve Regeneration in Rats

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Yuhui Kou ◽  
Zhiyong Wang ◽  
Zhihong Wu ◽  
Peixun Zhang ◽  
Yu Zhang ◽  
...  
Keyword(s):  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Nerve Repair ◽  
Peripheral Nerve Regeneration ◽  
Control Group ◽  
Control Groups ◽  
Wet Weight ◽  
Sciatic Functional Index ◽  
And Control ◽  
Better Than

Effects ofEpimediumextract and its constituent icariin on peripheral nerve repair were investigated in a crush injury rat model. Animals were divided into four groups: sham, control,Epimediumextract, and icariin groups. At postoperative weeks 1, 2, 4, and 8, nerve regeneration and functional recovery were evaluated by sciatic functional index (SFI), nerve electrophysiology, nerve pinch test, and muscle wet weight. Results showed that at 2 and 4 weeks after surgery rats in theEpimediumgroup displayed a better recovery of nerve function than that in the icariin and control groups, with better recovery in the icariin group than in the control group. The nerve pinch test showed that nerve regeneration was greater in theEpimediumgroup and the icariin group as compared to the control group. In addition, the muscle wet weight in theEpimediumgroup was significantly improved when compared with the icariin group, and the improvement in the icariin group was better than that in the control group at 8 weeks after operation. Our findings suggest thatEpimediumextract effectively promotes peripheral nerve regeneration and improves the function of damaged nerves.

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