scholarly journals Screening of 20 Pantanal Wetland Plants for Anti-Candida Activity using HPLC-DAD-MS/MS and Bioautography to Characterize Active Compounds

2021 ◽  
Vol 8 (03) ◽  
pp. e96-e103
Author(s):  
Daniela Z. de Brito ◽  
Nadla S. Cassemiro ◽  
Jeana M. E. de Souza ◽  
Geraldo A. Damasceno-Junior ◽  
Rodrigo J. Oliveira ◽  
...  
Keyword(s):  
Inhibitory Concentration ◽  
Active Compounds ◽  
Antifungal Potential ◽  
Sesbania Virgata ◽  
Etoac Fraction ◽  
Pharmacological Potential ◽  
Rich Flora ◽  
Brazilian Pantanal ◽  
Podophyllotoxin Derivatives ◽  
Pantanal Wetland

AbstractThe Pantanal wetland harbors a rich flora with uncharted pharmacological potential. This study evaluated 20 Brazilian Pantanal plants against Candida albicans, C. parapsilosis, C. tropicalis, and C. krusei. Fungal susceptibility was determined by agar diffusion and broth microdilution; active compounds were identified by bioautography and HPLC-DAD-MS/MS. Sesbania virgata, Polygala molluginifolia, and Cantinoa mutabilis extracts and their chloroform and ethyl acetate (EtOAc) fractions exhibited the best activity against all Candida species tested. The EtOAc fraction of P. molluginifolia proved to be more efficient in inhibiting C. parapsilosis and C. krusei growth (Minimum inhibitory concentration of 125 and 62.5 μg/mL, respectively). Bioautography of this fraction revealed two active bands, characterized by HPLC-DAD-MS/MS as a mixture of podophyllotoxin derivatives blumenol, besides some flavonoids. This work demonstrated antifungal potential of P. molluginifolia podophyllotoxin derivatives and the versatility of bioautography with HPLC-DAD-MS/MS to identify the bioactive compounds.

scholarly journals Plant diversity of the Pantanal wetland

2011 ◽  
Vol 71 (1 suppl 1) ◽  
pp. 265-273 ◽  
Author(s):  
A Pott ◽  
AKM Oliveira ◽  
GA Damasceno-Junior ◽  
JSV Silva
Keyword(s):  
Plant Diversity ◽  
Wide Distribution ◽  
Riparian Forests ◽  
Endemic Plants ◽  
Vegetation Types ◽  
Large Area ◽  
Characteristic Species ◽  
Brazilian Pantanal ◽  
Pantanal Wetland ◽  
Seasonal Forests

This is a review of current studies in diversity of the flora and main vegetation types in the Brazilian Pantanal. The flora of this wetland, nearly 2,000 species, constitutes a pool of elements of wide distribution and from more or less adjacent phytogeographic provinces, such as Cerrado, dry seasonal forests, Chaco, Amazonia and Atlantic Forest. The most numerous group includes wide-distribution species, mainly herbs, while the second contingent comes from the Cerrado. Endemic plants are rare, numbering only seven. The vegetation of the sedimentary floodplain is a mosaic of aquatics, floodable grasslands, riparian forests, savannas (cerrados), cerrado woodlands, dry forests, and a large area of mono-dominant savannas, and pioneer woodlands. The main vegetation types are briefly described with their characteristic species, and their estimated areas are given according to the latest mapping.

Assessment of equine piroplasmids in the Nhecolândia sub-region of Brazilian Pantanal wetland using serological, parasitological, molecular, and hematological approaches

2019 ◽  
Vol 10 (3) ◽  
pp. 714-721 ◽  
Author(s):  
João B.V. Campos ◽  
Marcos R. André ◽  
Luiz R. Gonçalves ◽  
Carla R. Freschi ◽  
Filipe M. Santos ◽  
...  
Keyword(s):  
Brazilian Pantanal ◽  
Pantanal Wetland

scholarly journals Synthesis and Antimicrobial Resistant Modulatory Activity of 2,4-Dinitrophenylhydrazone Derivatives as Agents against Some ESKAPE Human Pathogens

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Alberta Ade ◽  
Cedric D. K. Amengor ◽  
Abena Brobbey ◽  
Isaac Ayensu ◽  
Benjamin K. Harley ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Klebsiella Pneumoniae ◽  
Inhibitory Concentration ◽  
Fungal Species ◽  
Human Pathogens ◽  
Visible Spectroscopy ◽  
Active Compounds ◽  
Gram Negative ◽  
Uv Visible

A library of six novel phenylhydrazones were synthesized and evaluated for their in vitro antimicrobial and resistance modulating activity against a panel of Gram-positive, Gram-negative, and fungal species. The compounds were produced in good yields of 60–92% w/w and characterized using melting point, UV-visible spectroscopy, infrared, and nuclear magnetic resonance (1H, 13C, and DEPT-Q) techniques. Mass spectroscopy was used to confirm the identity of one of the most active compounds, 5 [SA5]. The phenylhydrazones showed activity against all the six selected microorganisms with minimum inhibitory concentration (MIC) values of the most active compounds, 1 [BP1] and 5 [SA5], at 138 µM (Klebsiella pneumoniae) and 165 µM (Streptococcus pneumoniae), respectively. Compound 1 [BP1] further demonstrated a high resistance modulatory activity at 1.078 µM against Streptococcus pneumoniae and Klebsiella pneumoniae.

scholarly journals Changes in vegetation cover of the Pantanal wetland detected by Vegetation Index: a strategy for conservation

2018 ◽  
Vol 18 (1) ◽  
Author(s):  
Ciomara de Souza Miranda ◽  
Antonio Conceição Paranho Filho ◽  
Arnildo Pott
Keyword(s):  
Vegetation Cover ◽  
Transition Matrix ◽  
Vegetation Index ◽  
Flood Pulse ◽  
Hydrological Processes ◽  
Wetland Area ◽  
Dinamica Ego ◽  
Brazilian Pantanal ◽  
Pantanal Wetland

The Brazilian Pantanal wetland undergoes landscape alterations that can cause impacts on hydrological processes, changing the flood pulse. The objective of this work is to analyse the vegetation cover of the Pantanal in the period of 2000, 2008 and 2015, and to make a projection for 2030. Therefore, NDVI from the sensor MODIS was analysed and the transition matrix was calculated by the DINAMICA EGO. The methods adopted were open sources. The results were worrisome, indicating alterations of the vegetation cover of the Pantanal, with an increase of short vegetation (grasslands or pastures) in the evaluated period. The projection pointed out that in 2030 the Brazilian Pantanal wetland area will be covered by 78% of short vegetation and only 14% of dense (arboreal-shrubby) vegetation. The approach can be a useful tool for conservation of the Brazilian Pantanal wetland.

scholarly journals EVALUATION OF ANTIFUNGAL POTENTIAL OF ALLIUM AMPELOPRASUM AGAINST FUNGAL PHYTOPATHOGENS

2018 ◽  
Vol 11 (11) ◽  
pp. 129
Author(s):  
Shahid Khan ◽  
Amandeep Kaur ◽  
Neeta Raj Sharma
Keyword(s):  
Chemical Composition ◽  
Antifungal Activity ◽  
Inhibitory Concentration ◽  
Economic Importance ◽  
Allium Ampeloprasum ◽  
Water Extracts ◽  
Maximum Inhibition ◽  
Antifungal Potential ◽  
Organic Extracts ◽  
Fungal Phytopathogens

Objective: Present study evaluates the antifungal potential of Allium ampeloprasum extracts against two phytopathogens of economic importance, namely Alternaria triticina and Magnaporthe oryzae which are found to be one of the greatest threats to wheat and rice crop worldwide.Methods: Four different organic solvents, i.e., water, methanol, acetone, and hexane were used for extract preparation based on the increasing order of polarity. Further, different concentrations of these extracts were used to perform a quantitative assay for measuring the antifungal activity of extracts. Two-way analysis of variance was used to statistically analyze the results through GraphPad Prism v. 7.00.Results: The best results were shown by water extracts with a maximum inhibition of 99.39±0.2% against A. triticina and 99.39±2.2% against M. oryzae. Water extracts gave minimal inhibitory concentration 90 values at 9.94 μg/mL against M. oryzae and at 16.73 μg/mL against A. triticina.Conclusion: Water extracts of A. ampeloprasum have relatively higher potential for being used as an antifungal agent as compared to other organic extracts used in this study. Further study on the chemical composition of A. ampeloprasum water extracts can reveal potent anti-phytopathogenic fungal compounds which can be used to develop biofungicides.

scholarly journals Attempts to Access a Series of Pyrazoles Lead to New Hydrazones with Antifungal Potential Against Candida Species Including Azole-Resistant Strains

2021 ◽  
Author(s):  
Georgiana NEGRU ◽  
Laure KAMUS ◽  
Elena Bîcu ◽  
Sergiu Shova ◽  
Boualem SENDID ◽  
...  
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Antifungal Agents ◽  
Inhibitory Concentration ◽  
Pyrazole Derivatives ◽  
Mammalian Cell Line ◽  
Cell Toxicity ◽  
Candida Spp ◽  
X Ray ◽  
Antifungal Potential ◽  
Resistant Strains

The treatment of benzylidenemalononitriles 3a-c with phenylhydrazines 4a-n in refluxing ethanol did not provide pyrazole derivatives but furnished hydrazones 1a-o. The structure of hydrazones was secured by X-Ray analysis. Newly synthesized hydrazones 1a-o were tested against 8 Candida spp. strains in a dose response assay to determine the minimum inhibitory concentration (MIC99). Five compounds 1c, 1d, 1i, 1k and 1l were identified as promising antifungal agents against Candida spp. (C. albicans SC5314, C. glabrata, C. tropicalis, C. parapsilosis and C. glabrata (R azoles)) with MIC99 values ranging from 16 to 32 µg/mL. To further evaluate the antifungal potential of the active compounds, they have been assayed against a mammalian cell line HEK293 to determine general cell toxicity and on NCI-60 cancer cell lines panel, demonstrating selectivity antifungal activity over cytotoxicity.

scholarly journals Osteogenesis activity of fractions extracted from Clinacanthus nutans (Burm. F.) Lindau

2020 ◽  
Vol 42 (2) ◽  
Author(s):  
Pham Thi Thu Phuong ◽  
Nguyen Thi Hong Minh ◽  
Quach Thi Lien ◽  
Nguyen Thi Thanh Huong ◽  
Nguyen Thi Mai Phuong
Keyword(s):  
Alkaline Phosphatase ◽  
Ethyl Acetate ◽  
In Vitro Model ◽  
Extensive Investigation ◽  
Osteoblast Cells ◽  
Active Compounds ◽  
Etoac Fraction ◽  
Clinacanthus Nutans ◽  
Vitro Model

Osteogenesis activity of fractions extracted from Clinacanthus nutans was evaluated on an in vitro model using osteoblast MC3T3-E1 cells. The results showed that all fractions, including ethanol (EtOH), n-hexane, ethyl acetate (EtOAc) and butanol (BuOH) were not significantly toxic to the osteoblast cells at the test concentrations of 5, 10, 25, and 50 µg/mL. The EtOH and EtOAc fractions exhibited the highest osteogenesis activity in terms of enhancement of alkaline phosphatase (ALP) and mineralization activity of MC3T3-E1 cells, especially the EtOAc fraction which increased ALP activity up to > 30% and mineralization activity up to > 100%. Thus, the EtOAc fraction shows osteogenesis activity through stimulating activites of the two markers for bone generation including ALP and mineralization in osteoblast cells. The fraction is now under extensive investigation to isolate and fully understand the modes of action of the active compounds.  

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