HPLC analysis of flavonoids of Astragalus gossypinus (Fabaceae), as a medicinal plant in the West of Iran

Planta Medica ◽  
2009 ◽  
Vol 75 (09) ◽  
Author(s):  
M Atri ◽  
M Asgari Nematin ◽  
E Tamari
Keyword(s):  
Medicinal Plant ◽  
Hplc Analysis ◽  
The West

scholarly journals Variation in phytochemical properties and expression of key genes involved in biosynthesis of hypericin using nano-capsulated and normal hormones in Hypericum perforatum L

2021 ◽  
Author(s):  
Leila Ghodrati ◽  
Mehrdad Ataie Kachoie ◽  
Shima Gharibi
Keyword(s):  
Normal Form ◽  
Medicinal Plant ◽  
Hplc Analysis ◽  
Hypericum Perforatum ◽  
Expression Patterns ◽  
Qrt Pcr ◽  
Germacrene D ◽  
Hypericum Perforatum L ◽  
Key Genes

Abstract Hypericum perforatum is a valuable medicinal plant with anti-depressant activity. Hypericin is the major compound responsible for such activity. In the present study, the effect of four nano-capsulated hormones of 2,4-epibrassinolide, spermidine, salycilic acid and cycocel were investigated on the amount of hypericin based on HPLC analysis in two locations (Saman and Isfahan). For each hormone, the normal form was also compared with nano form. The expression patterns of the key genes (Hyp-1, pks1, pks2) for hypericin production was also evaluated using qRT-PCR. Moreover, GC-MS analysis was also performed for determination of the compounds in studied treatments. The major compounds were germacrene D (3.29–33.53%), β-caryophyllene (0-4.08) and α-longipinene (0-24.05%). In most cases, nano-hormones led to increase in these components. Significant changes were obtained in expression of key genes in hypericin synthesis as a result of nano-hormones treatments in Isfahan site. Overall, nano-hormones revealed higher increase in expression of all genes as compared with normal hormones in this site. The expression of Hyp-1, Pks1 and Pks2 was significantly increased using spermidine, 24-epibrassinolide and cycocel in Isfahan location in both nano-hormones and normal ones, while the expression of Hyp-1 was decreased in SA treatment in Isfahan location. Based on HPLC analysis, hypericin ranged from 0.21 in control to 0.51 mg 100 g− 1 DW in nano-SA in Isfahan site. Finally, the expression of the key genes were mostly elevated in colder climates and nano-form formulation.

Antioxidant activity of prenylated flavonoids from the West African medicinal plant Dorstenia mannii

2003 ◽  
Vol 87 (1) ◽  
pp. 67-72 ◽  
Author(s):  
K.G. Dufall ◽  
B.T. Ngadjui ◽  
K.F. Simeon ◽  
B.M. Abegaz ◽  
K.D. Croft
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plant ◽  
West African ◽  
The West ◽  
Prenylated Flavonoids

scholarly journals Etnomedisin masyarakat Warsamdin, Kepulauan Raja Ampat, Papua Barat

2018 ◽  
Vol 2 (1) ◽  
pp. 50-58
Author(s):  
Sudarmono Sudarmono
Keyword(s):  
Medicinal Plants ◽  
Medicinal Plant ◽  
Traditional Medicine ◽  
Key Words ◽  
Traditional Knowledge ◽  
Plant Species ◽  
The West ◽  
West Papua ◽  
Papua Barat

Waigeo Island people in Warsamdin Village are remote from the West Papua mainland. But now transportation is easier after becoming a maritime tourism destination for the international community and traditional influences turn out to be modern as is the use of traditional medicine. Traditional knowledge about medicines still relies naturally on utilizing medicinal plants. This study aims to explore the potential of medicinal plants in the Warsamdin community, Waigeo Island, West Papua for generations. The method used by interviewing indigenous elders known as Old Man is either the local name of a medicinal plant, how to use it and as a traditional medicine and traced the morphology of the plant species. Local names are some commonly used species, namely Min ikanu (Morinda citryfolia), Kanaul leaves (Hibiscus sp), Deawas (Psidium guava), red fruit (Pandanus conoideus), Kokanu kaluku, Kiom ikanui, Umare ukani, Bim ikani, Wit ikani , Kokanu matalu. Kabaji and Gaviol. Knowledge of medicinal plants is a knowledge gained through the use of local customs that are descended from the origin of the tribe from Biak Island. These limitations are also influenced by the species of medicinal plants available in the area. The role of medicinal plants is still believed to be able to cure diseases and help cure pain.   Key words: etnomedicine, Warsamdin village, Waigeo island, Raja Ampat, West Papua

scholarly journals Identification and quantitative determination of arbutin in the herb of Orthilia secunda

2021 ◽  
Vol 10 (4) ◽  
pp. 122-128
Author(s):  
A. V. Lezina ◽  
I. I. Terninko ◽  
M. V. Krysko
Keyword(s):  
Medicinal Plant ◽  
Quantitative Determination ◽  
Gallic Acid ◽  
Hplc Analysis ◽  
Spectral Characteristics ◽  
Array Detector ◽  
Hydrolytic Cleavage ◽  
Anti Inflammatory ◽  
Identification And Quantification

Introduction. Orthilia secunda (L.) House is a perennial herb that grows in Europe, Siberia, Asia Minor and Central Asia. The herb of Orthilia secunda is actively used in folk medicine as a diuretic, wound-healing and anti-inflammatory agent. From literary sources it is known that this medicinal plant raw material (PRM) contains flavonoids, tannins, organic acids, vitamins, as well as simple phenols and their derivatives (arbutin and hydroquinone). The presence of arbutin is responsible for the plant's high antioxidant and anti-inflammatory properties. But the use of Orthilia secunda in official medicine is limited due to the lack of complete information on the chemical composition and criteria for standardization of this type of medicinal product.Aim. Identification and quantification of arbutin by chromatographic methods in Orthilia secunda (L.) House, harvested in various phytocenotic zones.Materials and methods. The investigated medicinal plant material – the herb of Orthilia secunda – was harvested in various phytocenotic zones: in July 2018, harvesting was carried out in the northern part of Kazakhstan (Kokshetau district), in July-August 2019 in the Perm Territory and in the Tyumen Region. Preliminary identification of arbutin and related phenols – gallic acid and hydroquinone – was carried out by high performance thin layer chromatography (HPTLC) on a CAMAG instrument with a UV cabinet (Merck HPTLC silica gel 60 F154 plates, 20 × 10), semi-automatic Linomat 5 applicator (sample application). Elution of the plates was performed in a CAMAG Automatic Developing Chamber (ADC2). Image fixation was performed on a CAMAG Scanner 3 spectrodensitometer. The quantitative determination of arbutin was carried out by the method of highperformance liquid chromatography, which was carried out on a Prominence LC-20 device (Shimadzu, Japan) according to the validated method described in the European Pharmacopoeia 10.0. Diode array detector SPD-M20A, column Intersil C18 column (250–4.6 mm, 5 μm) (Phenomenex, USA). The results were processed using the LabSolution software. The identification and quantification of arbutin was carried out in comparison with a standard solution containing a reference sample (RS) of arbutin (C = 0,025 mg/ml) and RS of hydroquinone (C = 0,0125 mg/ml).Results and discussion. HPTLC analysis made it possible to detect arbutin and gallic acid – the main product of hydrolytic degradation/ precursor of the biosynthesis of tannins of the hydrolysable group – in the herb of Orthilia secunda from different places of growth. HPLC analysis demonstrates a different chromatographic profile of Orthilia herb harvested in different phytocenotic zones. However, in all studied objects, the absence of hydroquinone and the presence of substances that can presumably be attributed to its derivatives were confirmed, which is confirmed by the visual similarity of the spectra of these compounds and the proximity of the extrema. It was found that arbutin does not belong to the marker (majority) compounds of Orthilia. Its content is low and reaches a maximum (about 0,021 %) in the herb of Orthilia secunda growing on the territory of Kazakhstan, while in the herb of Orthilia harvested in the Perm Territory arbutin was not identified. From the data obtained, it follows that the greatest accumulation of arbutin occurs in areas with a warmer and drier climate (northern part of Kazakhstan).Conclusion. HPTLC analysis of the herb Orthilia secunda allowed the identification of arbutin and gallic acid (the main precursor of tannins of the hydrolysable group). The results of HPLC analysis of Orthilia herb harvested in various phytocenotic zones suggest quantitative differences in the content of arbutin depending on the region of growth. From the experimental data, it follows that Orthilia growing in the northern part of Kazakhstan accumulates the maximum (0,021%) amount of arbutin, in comparison with the samples harvested in the Tyumen region and the Perm region. At the same time, Orthilia harvested in the Perm Territory does not accumulate arbutin. The presence of hydroquinone has not been confirmed (by HPTLC and HPLC methods); therefore, it is not justified to talk about the hydrolytic cleavage of arbutin in the process of biosynthesis or drying. However, in all studied objects there are peaks of substances with spectral characteristics like hydroquinone, which makes it possible to assume the presence of its derivatives. Therefore, it is not advisable to position arbutin as a marker compound of Orthilia secunda harvested on the territory of the Russian Federation, and to standardize raw materials for this compound.

Enumeration and Identification of Asbestos Fibres in Water

1974 ◽  
Vol 32 ◽  
pp. 526-527 ◽  
Author(s):  
O. Mudroch ◽  
J. R. Kramer
Keyword(s):  
Water Supply ◽  
Water Samples ◽  
Drainage Basin ◽  
Lake Superior ◽  
Research Work ◽  
List Type ◽  
The West ◽  
Asbestos Fibre ◽  
Amphibole Asbestos

Approximately 60,000 tons per day of waste from taconite mining, tailing, are added to the west arm of Lake Superior at Silver Bay. Tailings contain nearly the same amount of quartz and amphibole asbestos, cummingtonite and actinolite in fibrous form. Cummingtonite fibres from 0.01μm in length have been found in the water supply for Minnesota municipalities.The purpose of the research work was to develop a method for asbestos fibre counts and identification in water and apply it for the enumeration of fibres in water samples collected(a) at various stations in Lake Superior at two depth: lm and at the bottom.(b) from various rivers in Lake Superior Drainage Basin.

The fight to save Uganda’s White Rhino

1964 ◽  
Vol 2 (01) ◽  
pp. 6-12
Keyword(s):  
The West ◽  
West Nile ◽  
White Rhino

In the West Nile District of Uganda lives a population of white rhino—those relies of a past age, cumbrous, gentle creatures despite their huge bulk—which estimates only 10 years ago, put at 500. But poachers live in the area, too, and official counts showed that white rhino were being reduced alarmingly. By 1959, they were believed to be diminished to 300.

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