Gene screening and metabolomics for identification of halogenated natural products from marine sponge-associated bacteria

Planta Medica ◽  
2015 ◽  
Vol 81 (11) ◽  
Author(s):  
S Goldberg ◽  
B Haltli ◽  
F Berrue ◽  
R Kerr
Keyword(s):  
Natural Products ◽  
Marine Sponge ◽  
Associated Bacteria ◽  
Gene Screening ◽  
Sponge Associated Bacteria ◽  
Halogenated Natural Products

Fatty Acid Profile and In Vitro Anticancer Activity of Two Marine Sponge- Associated Bacteria

2020 ◽  
Vol 16 (9) ◽  
pp. 1273-1280
Author(s):  
Giuseppina Tommonaro ◽  
Ali M. El-Hagrassi ◽  
Walid Fayad ◽  
Carmine Iodice ◽  
Kamel H. Shaker ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Fatty Acids ◽  
Fatty Acid ◽  
Colorectal Carcinoma ◽  
Cell Line ◽  
Marine Sponge ◽  
Hct116 Cell ◽  
Associated Bacteria ◽  
Sponge Associated Bacteria ◽  
Human Colorectal Carcinoma

Background: Colorectal cancer represents one of the prominent causes of mortality worldwide in men and women. The objective of this study was to search for new potential anticancer compounds, both in prevention and treatment of colorectal cancer. The anticancer potential of marine bacterial extracts against Human colorectal carcinoma cell line (HCT116) was evaluated as well as the partial identification of bioactive metabolites. Methods: All bacterial extracts were tested for their cytotoxicity against HCT116 cell line by means of MTT assay. The highly cytotoxic dichloromethane extracts of marine sponge-associated bacteria Vibrio sp. and Bacillus sp. were analyzed by GC-MS. Results: Two fractions, Vib3 and Bac3, exhibited a very interesting cytotoxicity against human colorectal carcinoma (HCT116) cell line, with a percentage of cytotoxicity of 96.04 % and 29.48 %, respectively. Discussion: The GC-MS analysis revealed the presence of two major fatty acids, palmitic and oleic acids, in Vib3 fraction and fatty acid esters and phenolic compounds in Bac3 fraction. Conclusion: Based on previous literature, it may be hypothesized that the anticancer activity of bacterial extracts could be, at least partially, to the fatty acids fraction.

scholarly journals Screening of marine sponge-associated bacteria from Echinodictyum gorgonoides and its bioactivity

2012 ◽  
Vol 11 (88) ◽  
pp. 15469-15476 ◽  
Author(s):  
Isaac Dhinakaran D ◽  
Ramakrishana Deva Prasad D ◽  
Gohila R ◽  
P Lipton A
Keyword(s):  
Marine Sponge ◽  
Associated Bacteria ◽  
Sponge Associated Bacteria

Isolation and Characterization of a GDSL Esterase from the Metagenome of a Marine Sponge-associated Bacteria

2009 ◽  
Vol 12 (4) ◽  
pp. 395-402 ◽  
Author(s):  
Yoshiko Okamura ◽  
Tomonori Kimura ◽  
Hiroko Yokouchi ◽  
Macarena Meneses-Osorio ◽  
Masaya Katoh ◽  
...  
Keyword(s):  
Marine Sponge ◽  
Associated Bacteria ◽  
Isolation And Characterization ◽  
Sponge Associated Bacteria

Host specificity in marine sponge-associated bacteria, and potential implications for marine microbial diversity

2003 ◽  
Vol 6 (2) ◽  
pp. 121-130 ◽  
Author(s):  
Michael W. Taylor ◽  
Peter J. Schupp ◽  
Ingela Dahllöf ◽  
Staffan Kjelleberg ◽  
Peter D. Steinberg
Keyword(s):  
Microbial Diversity ◽  
Host Specificity ◽  
Marine Sponge ◽  
Associated Bacteria ◽  
Sponge Associated Bacteria ◽  
Marine Microbial Diversity

Investigating on the Correlation Between Some Biological Activities of Marine Sponge-Associated Bacteria Extracts and Isolated Diketopiperazines

2016 ◽  
Vol 74 (1) ◽  
pp. 6-13 ◽  
Author(s):  
Faten K. Abd El-Hady ◽  
Walid Fayad ◽  
Carmine Iodice ◽  
Zeinab A. El-Shahid ◽  
Mohamed S. Abdel-Aziz ◽  
...  
Keyword(s):  
Marine Sponge ◽  
Biological Activities ◽  
Associated Bacteria ◽  
Sponge Associated Bacteria

scholarly journals Bioactivity Screening and Gene-Trait Matching across Marine Sponge-Associated Bacteria

Marine Drugs ◽  
2021 ◽  
Vol 19 (2) ◽  
pp. 75
Author(s):  
Asimenia Gavriilidou ◽  
Thomas Andrew Mackenzie ◽  
Pilar Sánchez ◽  
José Ruben Tormo ◽  
Colin Ingham ◽  
...  
Keyword(s):  
Natural Products ◽  
Gene Clusters ◽  
Marine Sponges ◽  
Genetic Associations ◽  
Bioactive Substances ◽  
Associated Bacteria ◽  
Peptide Synthetases ◽  
Sponge Associated Bacteria ◽  
High Cytotoxicity

Marine sponges harbor diverse microbial communities that represent a significant source of natural products. In the present study, extracts of 21 sponge-associated bacteria were screened for their antimicrobial and anticancer activity, and their genomes were mined for secondary metabolite biosynthetic gene clusters (BGCs). Phylogenetic analysis assigned the strains to four major phyla in the sponge microbiome, namely Proteobacteria, Actinobacteria, Bacteroidetes, and Firmicutes. Bioassays identified one extract with anti-methicillin-resistant Staphylococcus aureus (MRSA) activity, and more than 70% of the total extracts had a moderate to high cytotoxicity. The most active extracts were derived from the Proteobacteria and Actinobacteria, prominent for producing bioactive substances. The strong bioactivity potential of the aforementioned strains was also evident in the abundance of BGCs, which encoded mainly beta-lactones, bacteriocins, non-ribosomal peptide synthetases (NRPS), terpenes, and siderophores. Gene-trait matching was performed for the most active strains, aiming at linking their biosynthetic potential with the experimental results. Genetic associations were established for the anti-MRSA and cytotoxic phenotypes based on the similarity of the detected BGCs with BGCs encoding natural products with known bioactivity. Overall, our study highlights the significance of combining in vitro and in silico approaches in the search of novel natural products of pharmaceutical interest.

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