Kinin-like Activities of the Synthetic Low Molecular Weight Fragment of Fibrinogen Degradation Products

1974 ◽  
Vol 32 (02/03) ◽  
pp. 350-355
Author(s):  
Akira Takaki ◽  
Tsuyoshi Yamaguchi ◽  
Keiichi Ohsato
Keyword(s):  
Molecular Weight ◽  
Smooth Muscle ◽  
Muscle Contraction ◽  
Synthetic Peptide ◽  
Degradation Products ◽  
Hypotensive Effect ◽  
Smooth Muscle Contraction ◽  
Biological Properties ◽  
Low Molecular Weight ◽  
Fibrinogen Degradation Products

SummarySome biological properties of a synthetic peptide based on an anticoagulant peptide obtained from fibrinogen degradation products by the action of plasmin are reported. This peptide produced smooth muscle contraction without the presence of bradykinin. The contraction caused by this peptide was not modified by atropin (10-6 g/ml), hyoscine (10-6 g/ml) and tetrodotoxin (2xl0-7 g/ml). A hypotensive effect was observed following the intravenous injection of the peptide into an anesthetized rat indicating that this peptide has an action similar to that of bradykinin though less potent.

Low Molecular Weight Fibrinogen Degradation Products Stimulate the Release of Growth Factors from Endothelial Cells

1992 ◽  
Vol 68 (03) ◽  
pp. 357-363 ◽  
Author(s):  
Roberto Lorenzet ◽  
Joan H Sobel ◽  
Alessandra Bini ◽  
Larry D Witte
Keyword(s):  
Molecular Weight ◽  
Endothelial Cells ◽  
Growth Factors ◽  
Degradation Products ◽  
Cell Damage ◽  
Low Molecular Weight ◽  
Aortic Endothelial Cells ◽  
Fibrinogen Degradation Products ◽  
3T3 Fibroblasts ◽  
Growth Assay

SummaryCultured porcine aortic endothelial cells (PAEC) constitutively produce and secrete in their culture medium mitogens collectively called endothelial cell-derived growth factors (EDGFs). Incubation of PAEC with fibrinogen-degradation products (FDPs) obtained by plasmin digestion of highly purified fibrinogen caused an increased release of EDGFs, as assessed by [3H]-thymidine incorporation in 3T3 mouse fibroblasts. The effect was time-dependent and correlated with the degree of fibrinogenolysis. It was accompanied by elongation of the cells. Neither increase in EDGFs release nor cell damage was observed when non-degraded fibrinogen was incubated with endothelial cells. Low molecular weight fibrinogen degradation products (LMWFDPs) (M r ≤ 10,000), and the higher molecular weight fibrinogen fragments D and E were tested under the same conditions. Only the LMWFDPs caused elongation and damage to PAEC and a marked stimulation (up to 12 fold) of EDGFs release. A low density growth assay revealed that the released EDGFs were mitogenically active on the same PAEC. The activity of the released EDGFs was time and dose dependent on both 3T3 fibroblasts and PAEC, indicating that LMWFDPs caused enhanced release of EDGFs that can act in paracrine and autocrine fashion. This study suggests an additional role for fibrinogenolysis contributing to wound healing, and possibly to atherosclerosis.

Suppressive effect of low molecular weight fibrinogen degradation products on human and rat lymphocytes

1978 ◽  
Vol 12 (3) ◽  
pp. 523-530 ◽  
Author(s):  
K. Krzystyniak ◽  
J. Stachurska ◽  
J. Ryżewski ◽  
K. Bykowska ◽  
M. Kopeć
Keyword(s):  
Molecular Weight ◽  
Degradation Products ◽  
Suppressive Effect ◽  
Low Molecular Weight ◽  
Fibrinogen Degradation Products ◽  
Rat Lymphocytes

scholarly journals Reduced biomechanical models for precision-cut lung-slice stretching experiments

2021 ◽  
Vol 82 (5) ◽  
Author(s):  
Hannah J. Pybus ◽  
Amanda L. Tatler ◽  
Lowell T. Edgar ◽  
Reuben D. O’Dea ◽  
Bindi S. Brook
Keyword(s):  
Smooth Muscle ◽  
Muscle Contraction ◽  
Ex Vivo ◽  
Finite Thickness ◽  
Biomechanical Model ◽  
Local Stress ◽  
Smooth Muscle Contraction ◽  
Biomechanical Models ◽  
Cytokine Activation ◽  
Asymptotic Reduction

AbstractPrecision-cut lung-slices (PCLS), in which viable airways embedded within lung parenchyma are stretched or induced to contract, are a widely used ex vivo assay to investigate bronchoconstriction and, more recently, mechanical activation of pro-remodelling cytokines in asthmatic airways. We develop a nonlinear fibre-reinforced biomechanical model accounting for smooth muscle contraction and extracellular matrix strain-stiffening. Through numerical simulation, we describe the stresses and contractile responses of an airway within a PCLS of finite thickness, exposing the importance of smooth muscle contraction on the local stress state within the airway. We then consider two simplifying limits of the model (a membrane representation and an asymptotic reduction in the thin-PCLS-limit), that permit analytical progress. Comparison against numerical solution of the full problem shows that the asymptotic reduction successfully captures the key elements of the full model behaviour. The more tractable reduced model that we develop is suitable to be employed in investigations to elucidate the time-dependent feedback mechanisms linking airway mechanics and cytokine activation in asthma.

scholarly journals Involvement of rho p21 in the GTP-enhanced calcium ion sensitivity of smooth muscle contraction.

1992 ◽  
Vol 267 (13) ◽  
pp. 8719-8722 ◽  
Author(s):  
K Hirata ◽  
A Kikuchi ◽  
T Sasaki ◽  
S Kuroda ◽  
K Kaibuchi ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Muscle Contraction ◽  
Calcium Ion ◽  
Smooth Muscle Contraction
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