Sensitivity of Pregnant Mice to Intravenous Tissue Thromboplastin

1969 ◽  
Vol 22 (01) ◽  
pp. 035-044
Author(s):  
S Coccheri ◽  
P Ollendorff ◽  
T Astrup
Keyword(s):  
Protease Inhibitor ◽  
Intravenous Injection ◽  
Trypsin Inhibitor ◽  
Neurological Symptoms ◽  
Toxic Effects ◽  
Fibrinogen Concentration ◽  
Slow Process ◽  
Pregnant Mice ◽  
Increased Sensitivity ◽  
Tissue Thromboplastin

SummaryPregnant mice are more susceptible to the intravenous injection of tissue thromboplastin than nonpregnant mice. This was true whether death of the animal, or the appearance of neurological symptoms, was used as indicator of toxicity. Increase of fibrinogen concentration or prior injection of plasmin or of a protease inhibitor (trypsin inhibitor from bovine lung) did not influence the immediate toxic effects. The reason for the increased sensitivity of pregnant mice to tissue thromboplastin remains undisclosed. Inhibition of tissue thromboplastin by serum is a slow process. The reported increase in inhibition of tissue thromboplastin by serum from pregnant individuals could not be confirmed.

Ultrastructural Changes of the Tissue Thromboplastin after Intravenous Injection in Rabbits

1978 ◽  
Vol 39 (01) ◽  
pp. 201-209 ◽  
Author(s):  
Hiroshi Hasegawa ◽  
Hiroshi Nagata ◽  
Makoto Murao
Keyword(s):  
Intravenous Injection ◽  
Vascular Endothelium ◽  
Model Experiment ◽  
Venous Return ◽  
Ultrastructural Changes ◽  
Membrane Structures ◽  
Tissue Thromboplastin ◽  
Short Time ◽  
Sheet Membrane

SummaryAttempts were made to demonstrate ultrastructural changes of the tissue thromboplastin after intravenous injection, as a model experiment on the pulmonary microthrombi formation induced by the tissue thromboplastin circulating from venous return.Concentrically arranged membrane structures of the injected thromboplastin disappeared in extremely short time after the injection of the thromboplastin in rabbits. The long sheet membrane of the injected thromboplastin was frequently seen as adhered to the vascular endothelium or to the surface of blood corpuscles. Furthermore, fibrin fibres were formed in contact with the long sheet membrane of the thromboplastin. Membrane structures were not found anywhere in the control rabbits.

scholarly journals Expression of inter-alpha-trypsin inhibitor heavy chains in endometrium of cyclic and pregnant gilts

Reproduction ◽  
2003 ◽  
pp. 621-627 ◽  
Author(s):  
RD Geisert ◽  
MD Ashworth ◽  
Keyword(s):  
Gene Expression ◽  
Extracellular Matrix ◽  
Western Blot ◽  
Protease Inhibitor ◽  
Early Pregnancy ◽  
Trypsin Inhibitor ◽  
Serine Protease Inhibitor ◽  
Oestrous Cycle ◽  
Heavy Chains ◽  
Formation Of Complexes

Attachment of the placenta to the uterus in pigs involves extracellular interaction between the expanding trophoblastic membrane and the thick glycocalyx present on the uterine epithelial microvilli. Formation of complexes between members of inter-alpha-trypsin inhibitor family may function in the maintenance of the extracellular matrix. This study investigated the change in the inter-alpha-trypsin inhibitor heavy chains (ITIH1, ITIH2, ITIH3 and ITIH4) during the oestrous cycle and early pregnancy in pigs. Gene expression of ITIH1, ITIH2, ITIH3 and ITIH4 was detected in the endometrium of cyclic and pregnant gilts; however, gene expression of ITIH was not altered throughout the oestrous cycle or early pregnancy. Western blot analysis with an ITIH antiserum identified the possible linkage forms of ITIH with the serine protease inhibitor, bikunin. Pregnancy altered the release of the various inter-alpha-inhibitor forms from the endometrium during the period of trophoblastic attachment. The results from this study indicate that the inter-alpha-trypsin inhibitor family plays an important role in maintenance of the uterine surface glycocalyx during placental attachment in pigs.

Blut-Hirn-Schranke und Placentar-Schranke für H-Thymidin und H-Cytidin bei der Maus / Blood-Brain-Barrier and Placental-Barrier for H-Thymidine and H-Cytidine in the Mouse

1972 ◽  
Vol 27 (5) ◽  
pp. 554-558b ◽  
Author(s):  
B. Schultze ◽  
N. Hörning ◽  
W. Maurer
Keyword(s):  
Blood Brain Barrier ◽  
Intravenous Injection ◽  
Brain Barrier ◽  
Whole Body ◽  
Placental Barrier ◽  
Whole Body Autoradiography ◽  
Blood Brain ◽  
Pregnant Mice ◽  
Distribution In The Organism

The distribution in the organism of the mouse of free 3H-thymidine and 3H-cytidine was studied 1, 2½, 5 and 15 minutes after intravenous injection into normal and pregnant mice (20th day) using whole body autoradiography. The grain density measured over brain and fetus is 10 times smaller as compared to other tissues of the organism. This means that a blood brain barrier and placental barrier for thymidine and cytidine exists.

scholarly journals Enzymatic Cyclization of a Potent Bowman-Birk Protease Inhibitor, Sunflower Trypsin Inhibitor-1, and Solution Structure of an Acyclic Precursor Peptide

2003 ◽  
Vol 278 (24) ◽  
pp. 21782-21789 ◽  
Author(s):  
Ute C. Marx ◽  
Michael L. J. Korsinczky ◽  
Horst Joachim Schirra ◽  
Alun Jones ◽  
Barrie Condie ◽  
...  
Keyword(s):  
Protease Inhibitor ◽  
Trypsin Inhibitor ◽  
Solution Structure ◽  
Precursor Peptide ◽  
Acyclic Precursor

Fibrinogen synthesis in rabbits: effects of altered levels of circulating fibrinogen

1977 ◽  
Vol 232 (5) ◽  
pp. H478-H484
Author(s):  
B. M. Alving ◽  
W. R. Bell ◽  
B. L. Evatt
Keyword(s):  
Intravenous Injection ◽  
Degradation Products ◽  
Fibrinogen Concentration ◽  
Bovine Thrombin ◽  
Fibrin Degradation Products

The effects of altered fibrinogen concentrations on fibrinogen synthesis in rabbits were evaluated by determining the rate of appearance of [75Se]selenomethionine (75SeM) in circulating fibrinogen. Fibrinogen levels were maintained at twice normal by infusion of homologous fibrinogen for either 1 or 6 days before the intravenous injection of 20 micronCi of 75SeM, and the rate of appearance of labeled fibrinogen was measured during the subsequent 24 h. In both groups, synthesis was unchanged. Five hours after induction of partial defibrinogenation by the infusion of bovine thrombin, fibrinogen synthesis was increased threefold. Stimulation was not attributable to decreased fibrinogen concentrations; synthesis was increased equally when levels were maintained above normal by infusion of fibrinogen before administration of thrombin. Heat-inactivated thrombin and diisopropylfluorophosphate-inactivated thrombin did not stimulate fibrinogen synthesis. Thrombin produced elevated titers of fibrinogen-fibrin degradation products (FDP-fdp). However, fibrinogen synthesis was not increased in rabbits that had received FDP-fdp from thrombin-treated donors. These data suggest that neither the fibrinogen concentration nor FDP-fdp influenced fibrinogen synthesis.

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