Plasminogen Activity in Plasma and in Serum Before and After Venostasis

1979 ◽  
Author(s):  
C. Soria ◽  
A. Rodriguez-Zeballos ◽  
J. Soria ◽  
G. Kartalis
Keyword(s):  
Biological Activity ◽  
Synthetic Substrate ◽  
Lysis Time ◽  
Before And After ◽  
Euglobulin Lysis Time ◽  
Antigen Activity ◽  
Fibrin Clots

When venostasis is applied in men for 10 minutes at a pressure half-way between diastolic and systolic, various results may be obtained as far as fibrinolysis is concerned, normally the euglobulin lysis time reduced from above 3 hours to less than one hour. In these conditions, after venostasis the estimation of plasminogen after activation by Streptokinase (S.K.) on synthetic substrate S2251, shows 20-80% greater activity in serum than in plasma. In some patients at high risks of thrombosis, the euglobulin lysis time 15 unchanged after venostasis. In these cases, the biological activity of plasminogen is slightly greater in plasma than in serum, as is observed before venostasis in all subjects.In order to explain these observations, experiments were performed using placental or plasmatic (glu or lys) plasminogen ; these show that for the same antigen activity, the biological activity of placental plasminogen previously activated by S.K. is greater than that of plasmatic plasminogen. We suggest a modification of the structure of plasminogen in the presence of activators and of fibrin clots. Further experiments will be necessary to explain the modification of plasminogen structure

MODIFICATION OF COAGULATION AND FIBRINOLYSIS DURING TRANSLUMINAL ANGIOPLASTY IN PATIENTS RECEIVING HEPARIN OR PENTOSAN POLYSULFATE (HEMOCLAR)

1987 ◽  
Author(s):  
N Bel Lakhal ◽  
J M Pernes ◽  
D Lichtenstein ◽  
M Roncato ◽  
J C Gaux ◽  
...  
Keyword(s):  
Degradation Products ◽  
Transluminal Angioplasty ◽  
Thrombin Time ◽  
Pentosan Polysulfate ◽  
Heparin Group ◽  
Lysis Time ◽  
Before And After ◽  
Euglobulin Lysis Time ◽  
Thrombotic Complications ◽  
To Receive

Twenty patients with high stenosis of iliac or femoral artery were randomely allocated to receive by intra arterial route (IA) either 5,000 IU heparin or 50 mg Hemoclar immediately before starting the angioplasty. Those receiving Hemoclar were given a second IA 50 mg bolus at the end of the dilatation.Two blood samples were obtained by venous punction 1) after an initial 30 min resting (V1), 2) at the end of the procedure (V2)- Arterial punction by the dilatation catheter was also performed immediately before and after the dilatation leading to two other samples A1 and A2 The coagulation studies include activated partial thromboplastin time (APTT), thrombin time (TT), hep test (Hemachem, St-Louis, USA), anti Xa activity (Stachrom heparin, Diagnostica-Stago Asnieres, France). There was a significant increase in APTT, TT, and hep test between A1 and A2 as well as V1 and V2 in both groups of patients. However, the prolongation was significantly higher for heparin. Anti Xa activity significantly increased only in heparin group.Fibrinolysis was studied by measuring tPA by the SOFIA assay described by Angles-Cano (Anal. Biochem. 1985, 153, 201), euglobulin lysis time (ELT) and a new plasma ELISA assay specific for fibrinogen degradation products (FDPs) using monoclonal antibody described at the Gaubius Institute (Blood 1985, 66, 503). A significant increase in tPA was observed during the dilatation (A2/A1) and V2/V1) only in the patients receiving Hemoclar. The slight increase of the fibrinolytic activity was further corroborated by a significant increase in FDPs (A2/A1). In both groups, but only in the venous samples (V2/V1), ELT was shortened (p<0.05) and fibrinogen was decreased (p<0.05)No thrombotic complications were observed during the procedure in both groupsConclusion: This study confirms that Hemoclar has an inhibiting effect on coagulation by inhibiting thrombin and thrombin generation, but no anti Xa activity. However, the anticoagulant potency is much reduced when compared to heparin. The profibrinolytic effect seems to be related to the release of free tPA.

Venostasis but not DDAVP Infusion Provokes the Plasma Fibronectin Increase

1990 ◽  
Vol 64 (02) ◽  
pp. 294-296 ◽  
Author(s):  
M Letowska ◽  
K Bykowska ◽  
J Sablinski ◽  
S Lopaciuk ◽  
M Kopeć
Keyword(s):  
Von Willebrand Factor ◽  
Vessel Wall ◽  
Blood Donors ◽  
Plasma Fibronectin ◽  
Lysis Time ◽  
Before And After ◽  
Euglobulin Lysis Time ◽  
Von Willebrand ◽  
Willebrand Factor ◽  
Factor Antigen

SummaryPlasma fibronectin (pFN), von Willebrand factor antigen (vWf: Ag), factor VIII procoagulant activity, fibrinogen, euglobulin lysis time (ELT) and hematocrit were determined in healthy blood donors before and after venostasis as well as after intravenous infusion of l-deamino-8-D-arginine vasopressin (DDAVP). Both venostasis and DDAVP provoked an increase in vWf : Ag and shortening in the ELT. In contrast, venostasis only but not DDAVP induced an increase in pFN levels which was statistically significant with and without correction for a concomitant hematocrit increment. The results indicate that there is a distinct difference in the patterns of venostasis and DDAVP mediated release of proteins from the vessel wall.

The Amidolytic Activity of the SK-Plasminogen Complex Is Enhanced by a Potentiator which Is Generated in the Presence of Vascular Plasminogen Activator - Role of Fibrin Degradation Products

1982 ◽  
Vol 47 (03) ◽  
pp. 193-196 ◽  
Author(s):  
J Soria ◽  
C Soria ◽  
O Bertrand ◽  
F Dunn ◽  
M Samama ◽  
...  
Keyword(s):  
Plasminogen Activator ◽  
Degradation Products ◽  
Venous Stasis ◽  
Amidolytic Activity ◽  
Fibrin Degradation Products ◽  
Lysis Time ◽  
Before And After ◽  
Euglobulin Lysis Time ◽  
High Responders

SummaryIn the presence of an excess of streptokinase (SK) the amidolytic activity of the plasminogen-SK complex on chromogenic substrates is 12% lower in serum than in the corresponding plasma. However, in subjects in whom venous stasis lead to a shortening of the euglobulin lysis time to less than 60 min (high responders), the amidolytic activity of the plasminogen-SK complex in serum was 60% higher than in the corresponding plasma. Attempts to find alterations of the plasminogen molecule itself which would account for the enhanced activity in high responder serum were negative. No free plasmin was present and the plasminogens isolated from plasma and serum before and after venous stasis had the same amidolytic activity as glu-plasminogen in the presence of an excess of SK. N-terminal analysis of these four plasminogens revealed in each instance glutamic acid.The enhancement of the amidolytic activity of the SK-plasminogen complex in serum of high responders (potentiator activity) could be reproduced by adding purified tissue plasminogen activator (TA) to native blood before clotting, but not if TA was added to plasma or to prestasis serum. Removal of fibrin degradation products from poststasis serum resulted in the disappearance of potentiator activity. These experiments suggest that fibrin degradation products, generated during clotting in the presence of vascular or tissular plasminogen activator act as a potentiator of the amidolytic activity of the plasminogen SK-complex.

scholarly journals Factor VIII Response to Venous Congestion in Patients with von Willebrand’s Disease

1977 ◽  
Author(s):  
M. Morfini ◽  
G. Longo ◽  
P. Rossi Ferrini ◽  
A. Seri
Keyword(s):  
Gel Filtration ◽  
Normal Subjects ◽  
Vascular Wall ◽  
Venous Congestion ◽  
F Test ◽  
Elution Pattern ◽  
Lysis Time ◽  
Before And After ◽  
Glass Column ◽  
Euglobulin Lysis Time

Venous congestion(v.c.) is known to increase plasma levels of antihemophilic factor activity (VIII AHF) and fibrinolytic activity in man. In 20 normal subjects and 15 patients with VonWillebrand’s disease(VWD), before and after v.c. on forearm the VIII AHF and related antigen(VIII AGN), the euglobulin lysis time, the haematocrit and platelet’s count were performed. Ethanol-precipitate (v/v 3% at -3°C) is prepared from each sample. Two-dimensional Immunoelectrophoresis and gel filtration through siliconized glass column(K15/30 Pharmacia) packed with Sepharose 6B, was performed by applyng ethanol-precipitate. In normal subjects a significant increase of VIII AHF has been observed after v.c. (F test p<0.001) and of VIII AGN (F test p<0.05). In VWD patients only VIII AHF increase was found (F test p<0.01) but in patients with very low VIII AHF level, the increase did not occur. The anodal migration of AGN on crossed electrophoresis and elution pattern of AHF and AGN did not show any significant variations after v.c. in normal subjects as in VWD patients. A likely mechanism of VIII AHF and VIII AGN increase may be a release from endothelial cells of vascular wall during venous congestion.

Plasma Fibrinolytic Activity Following Oral Anabolic Steroid Therapy

1975 ◽  
Vol 34 (01) ◽  
pp. 236-245 ◽  
Author(s):  
I. D Walker ◽  
J. F Davidson ◽  
P Young ◽  
J. A Conkie
Keyword(s):  
Heart Disease ◽  
Ischaemic Heart Disease ◽  
Anabolic Steroid ◽  
Fibrinolytic Activity ◽  
Anabolic Steroids ◽  
Long Term Effects ◽  
Lysis Time ◽  
Detailed Evaluation ◽  
Euglobulin Lysis Time

SummarySix anabolic steroids were assessed for their ability to enhance plasma fibrinolytic activity in males with ischaemic heart disease. Five 17α-alkylated steroids (Ethyloestrenol, Norethandrolone, Methandienone, Methylandrostenediol and Oxymetholone) were examined and all produced a significant increase in plasma plasminogen activator as measured by the euglobulin lysis time. The only non-17α-alkylated steroid studied (Methenolone acetate) failed to enhance fibrinolysis. The 17α-alkylated steroids studied all deserve more detailed evaluation of their long term effects on plasma fibrinolytic activity.

Changes in Blood Coagulation and Fibrinolysis in Rats Fed Atherogenic Diets

1963 ◽  
Vol 10 (02) ◽  
pp. 295-308 ◽  
Author(s):  
Clarence Merskey ◽  
Herbert Wohl
Keyword(s):  
Blood Coagulation ◽  
Blood Platelets ◽  
Coagulation Factors ◽  
Atherogenic Diet ◽  
Normal Serum ◽  
Significant Prolongation ◽  
Lysis Time ◽  
Factor Ii ◽  
Euglobulin Lysis Time ◽  
Coagulation And Fibrinolysis

Summary1. Groups of rats were fed thrombogenic diets and the effects on blood coagulation and fibrinolysis assessed.2. Animals fed a diet containing cholesterol, thiouracil and cholic acid developed high levels of coagulation factors I, II, V, VII—X, VIII, IX and X.3. Animals fed a similar diet with additional 40% beef fat developed even greater elevation of V, VII—X, VIII and X, similar elevation of factor II, and lesser (but still significant) elevation of factors I and IX. In addition marked elevation of blood platelets occurred.4. Euglobulin lysis time of the group not fed the additional fat was longer than in controls. Significant prolongation of euglobulin lysis time was not found in the group fed additional fat.5. If the increased levels of plasma fibrinogen were taken into account, it was found that a larger amount of fibrin was lysed per unit time in the euglobulin lysis test with plasma from rats fed either atherogenic diet compared with controls.6. Defective thromboplastin generation was present in both groups of rats fed an atherogenic diet. The defect was present in the serum and was not due to lack of a factor required for thromboplastin generation. An inhibitor was present in the serum which was capable of preventing the action of normal serum.7. No good correlation was found between the occurrence of changes in blood coagulation or fibrinolysis and the presence or absence of thrombosis and infarction.8. The exact cause of these anomalies remains unexplained, as does the cause of the thrombosis in these animals. Starvation per se does not account for these abnormal findings. They could not adequately be explained on the basis of “hypercoagulability” of the blood.

Sequential Treatment of Deep Leg Vein Thrombosis with Porcine Plasmin and Low Dose Streptokinase

1982 ◽  
Vol 48 (02) ◽  
pp. 190-195 ◽  
Author(s):  
G A Marbet ◽  
R Eichlisberger ◽  
F Duckert ◽  
M A de Silva ◽  
L Biland ◽  
...  
Keyword(s):  
Low Dose ◽  
Significant Negative Correlation ◽  
Sequential Treatment ◽  
Factor V ◽  
Complete Dissolution ◽  
Vein Thrombosis ◽  
Time Treatment ◽  
Lysis Time ◽  
Euglobulin Lysis Time ◽  
Thrombolytic Effect

SummarySequential treatment of deep leg vein thrombosis with porcine plasmin and low dose streptokinase (10,000-20,000 U/h) produces strong systemic fibrinolysis as demonstrated by the sustained decrease of euglobulin lysis time, of thromboplastin time values in percent, fibrinogen and factor V levels. There is a statistically significant negative correlation between thrombolytic results and euglobulin lysis time. Treatment periods below 3 days are unlikely to give satisfactory results. Occluded vein segments with an apparent median age of 4 days including thrombi older than 10 days (20% of cases) are cleared with an average chance of 50%. Complete dissolution of all thrombi proximal to the crural veins has been demonstrated in 47/114 = 41.2%, some thrombolytic effect in 31/114 = 27.2% and treatment failure in 36/114 = 31.6%. The data favour laboratory monitoring of thrombolytic therapy.

Kaolin-Activated Euglobulin Lysis Time (KELT)

1979 ◽  
Author(s):  
H Greig
Keyword(s):  
No Value ◽  
Clinical Assessment ◽  
Fibrinolytic Activity ◽  
Factor Xii ◽  
Plasminogen Activation ◽  
Normal Range ◽  
Control Subjects ◽  
Lysis Time ◽  
Euglobulin Lysis Time ◽  
The Mean

The most commonly used test for clinical assessment of fibrinolytic activity is the Euglobulin Lysis Time (ELT). However the normal range is very wide, the long times are inconvenient and detection of inhibition is impossible. An attempt has been made to utilise the acceleration of the ELT when kaolin is present, to devise a test with shorter times, a narrower normal range, and better precision. The Euglobulin lysis time was carried out by a modification of the method of NILSSON and OLOW, after precipitation of the euglobulin in the absence of kaolin (ELT) and in the presence of 1 mg. kaolin/ml. plasma (KELT). In 14 control subjects the mean, SD, and range for the ELT were 168.6’, 54.6’, 84-290’; the corresponding values for the KELT were 60.3’, 8.3’ and 46-74’. However, it was found that there was no correlation between the ELT value and the corresponding KELT (’r’ = -0.021); on the contrary, the longer the ELT, the greater the shortening produced by kaolin and there is a direct correlation between the ELT and the shortening of the lysis time by kaolin; ’r’ = 0.988. It is concluded that the KELT has no value as a clinical measure of fibrinolytic activity; further, the results suggest that kaolin may remove an inhibitor(s) of plasminogen activation as well as initiating Factor XII - mediated plasminogen activation.

Euglobulin Lysis Time in Fresh and Stored Samples

1994 ◽  
Vol 102 (6) ◽  
pp. 794-796 ◽  
Author(s):  
Domenico Prisco ◽  
Rita Paniccia ◽  
Brunella Bandinelli ◽  
Monica Filippini ◽  
Tamara Brunelli ◽  
...  
Keyword(s):  
Lysis Time ◽  
Euglobulin Lysis Time

COAGULATION ABNORMALITIES IN LEAD EXPOSED RATS

1987 ◽  
Author(s):  
Narendra Kumar Satija ◽  
Har Bhajan Singh ◽  
Anjana Grover ◽  
Ram Mohan Rai
Keyword(s):  
Modern Technology ◽  
Environmental Pollutant ◽  
The Body ◽  
Albino Rats ◽  
Thrombin Time ◽  
Significant Prolongation ◽  
Lysis Time ◽  
Industrial Material ◽  
Euglobulin Lysis Time ◽  
Plasma Recalcification Time

The accelerated rate of development of modern technology has greatly expanded the range of health hazards. Lead, a widely used industrial material, is a significant environmental pollutant that contaminates food, water, soil and air. Although much progress has been made in elucidating its adverse effects on various systems of the body like hepatic, CNS, renal etc., its effect on coagulation remains to be established. In view of this an experimental study was carried out in animals to understand how lead influences hemostasis.Male albino rats were exposed to lead either acutely by administering 20 mg lead acetate per kg body weight daily i.p. for 3 days or chronically by administering lead through drinking water containing 5 ppm lead for 150 days. Acute exposure to lead caused severe coagulopathy characterized by significant prolongation of plasma recalcification time, decrease in platelet count and decreased wall adherence of blood, decreased fibrinogen and euglobulin lysis time and significant increase in prothrombin time, thrombin time, and partial thromboplastin time. Similar observations were found in chronically exposed animals. It is concluded that exposure to heavy metals like lead may lead to a state of hypocoagulability.

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