Pluripotency Evaluation of Newly Generated iPSC Lines using Chorioallantoic Membrane Assay

2020 ◽  
Author(s):  
M. Weber ◽  
J. Baur ◽  
H. Steinle ◽  
H. P. Wendel ◽  
M. Avci-Adali ◽  
...  
2017 ◽  
Vol 23 (12) ◽  
pp. 938-952 ◽  
Author(s):  
Inés Moreno-Jiménez ◽  
Janos M. Kanczler ◽  
Gry Hulsart-Billstrom ◽  
Stefanie Inglis ◽  
Richard O.C. Oreffo

Cells ◽  
2019 ◽  
Vol 8 (5) ◽  
pp. 440 ◽  
Author(s):  
Aoi Komatsu ◽  
Kotaro Matsumoto ◽  
Tomoki Saito ◽  
Manabu Muto ◽  
Fuyuhiko Tamanoi

Chorioallantoic membrane assay (CAM assay) using fertilized chicken eggs has been used for the study of tumor formation, angiogenesis and metastasis. Recently, there is growing realization that this system provides a valuable assay for a patient-derived tumor model. Several reports establish that tumor samples from cancer patients can be used to reproduce tumor in the chicken egg. High transplantation efficiency has been achieved. In this review, we discuss examples of transplanting patient tumors. We then discuss critical issues that need to be addressed to pursue this line of experiments. The patient-derived chicken egg model (PDcE model) has an advantage over other models in its rapid tumor formation. This raises the possibility that the PDcE model is valuable for identifying optimum drug for each individual patient.


2008 ◽  
Vol 14 (11-12) ◽  
pp. 705-714
Author(s):  
Giovana S. Di Marco ◽  
Antoine Alam ◽  
Frédéric Dol ◽  
Pierre Corvol ◽  
Jean-Marie Gasc ◽  
...  

PLoS ONE ◽  
2015 ◽  
Vol 10 (6) ◽  
pp. e0130935 ◽  
Author(s):  
Xue Xiao ◽  
Xiaoying Zhou ◽  
Huixin Ming ◽  
Jinyan Zhang ◽  
Guangwu Huang ◽  
...  

2017 ◽  
Vol 16 (6) ◽  
pp. 8781-8792 ◽  
Author(s):  
Wen-Ting Huang ◽  
Wei-Luan Cen ◽  
Rong-Quan He ◽  
You Xie ◽  
Yu Zhang ◽  
...  

2016 ◽  
Vol 11 (3) ◽  
pp. 621
Author(s):  
Nazia Tabassum ◽  
Alamgeer . ◽  
Abdul Aziz ◽  
Bashir Ahmad

<p class="Abstract">The present study was aimed to evaluate the effect of <em>Teucrium stocksianum</em> on angiogenesis by using chorioallantoic membrane (CAM) assay. Fertilized eggs were incubated on the 5<sup>th</sup> day and dose of different dilutions 0.03%, 0.05%, 0.1%, and 0.5% of the plant extract was applied on 6<sup>th</sup> day. Evaluation of primary, secondary and tertiary blood vessels diameter and CAM area on 7<sup>th</sup> day by SPIP software. <em>T. stocksianum</em> showed antiangiogenic effect by reducing the diameter of CAM of blood vessels by applying the dilutions while significant results were obtained at dilution of 0.5%.</p><p> </p><p><strong> </strong></p>


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