scholarly journals Evaluation of pharmacological effect of Teucrium stocksianum extract on angiogenesis using chorioallantoic membrane assay

2016 ◽  
Vol 11 (3) ◽  
pp. 621
Author(s):  
Nazia Tabassum ◽  
Alamgeer . ◽  
Abdul Aziz ◽  
Bashir Ahmad
Keyword(s):  
Blood Vessels ◽  
Plant Extract ◽  
Chorioallantoic Membrane ◽  
Pharmacological Effect ◽  
Cam Assay ◽  
Antiangiogenic Effect ◽  
Teucrium Stocksianum ◽  
Chorioallantoic Membrane Assay

<p class="Abstract">The present study was aimed to evaluate the effect of <em>Teucrium stocksianum</em> on angiogenesis by using chorioallantoic membrane (CAM) assay. Fertilized eggs were incubated on the 5<sup>th</sup> day and dose of different dilutions 0.03%, 0.05%, 0.1%, and 0.5% of the plant extract was applied on 6<sup>th</sup> day. Evaluation of primary, secondary and tertiary blood vessels diameter and CAM area on 7<sup>th</sup> day by SPIP software. <em>T. stocksianum</em> showed antiangiogenic effect by reducing the diameter of CAM of blood vessels by applying the dilutions while significant results were obtained at dilution of 0.5%.</p><p> </p><p><strong> </strong></p>

scholarly journals Patient Derived Chicken Egg Tumor Model (PDcE Model): Current Status and Critical Issues

Cells ◽  
2019 ◽  
Vol 8 (5) ◽  
pp. 440 ◽  
Author(s):  
Aoi Komatsu ◽  
Kotaro Matsumoto ◽  
Tomoki Saito ◽  
Manabu Muto ◽  
Fuyuhiko Tamanoi
Keyword(s):  
Cancer Patients ◽  
Chorioallantoic Membrane ◽  
Tumor Model ◽  
Tumor Formation ◽  
Current Status ◽  
Cam Assay ◽  
Chicken Egg ◽  
Critical Issues ◽  
Chicken Eggs ◽  
Chorioallantoic Membrane Assay

Chorioallantoic membrane assay (CAM assay) using fertilized chicken eggs has been used for the study of tumor formation, angiogenesis and metastasis. Recently, there is growing realization that this system provides a valuable assay for a patient-derived tumor model. Several reports establish that tumor samples from cancer patients can be used to reproduce tumor in the chicken egg. High transplantation efficiency has been achieved. In this review, we discuss examples of transplanting patient tumors. We then discuss critical issues that need to be addressed to pursue this line of experiments. The patient-derived chicken egg model (PDcE model) has an advantage over other models in its rapid tumor formation. This raises the possibility that the PDcE model is valuable for identifying optimum drug for each individual patient.

scholarly journals Chemical Composition of Juniperus Phoenicea and J. Drupacea Essential Oils and their Biological Effects in the Choriallantoic Membrane (CAM) Assay

2017 ◽  
Vol 12 (3) ◽  
pp. 1934578X1701200
Author(s):  
Aikaterini Koutsaviti ◽  
Olga Tzakou ◽  
Enza Maria Galati ◽  
Giovanna Certo ◽  
Maria Paola Germanò
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Topical Application ◽  
Biological Effects ◽  
Chorioallantoic Membrane ◽  
Cam Assay ◽  
Angiogenic Potential ◽  
Juniperus Phoenicea ◽  
Germacrene D ◽  
Chorioallantoic Membrane Assay

The aim of the present study was the chemical analysis of the essential oils from Juniperus phoenicea and J. drupacea female cones and evaluation of their biological effects. Fresh samples, collected in Greece, were subjected separately to hydrodistillation and the oils obtained analyzed by GC-FID and GC-MS. The oils were assessed using the CAM (chorioallantoic membrane) assay to evaluate their anti-angiogenic potential and the lack of irritant effects in topical application. GC analysis showed that mainly quantitative differences among the samples were observed: limonene was the most abundant compound in J. drupacea (27.0%) compared with J. phoenicea oil (1.6%); the content of α-pinene was high in both essential oils ( J. phoenicea 22.1%, J. drupacea 26.1%) followed by germacrene D ( J. phoenicea 7.4%, J. drupacea 7.1%, respectively). Nevertheless, qualitative differences were also detected as the diterpene 4- epi-abietal was present in a considerable amount (13.2%) in J. phoenicea essential oil, but was not detected in J. drupacea oil. In the CAM assay, only J. phoenicea essential oil evidenced a rather weak anti-angiogenic activity compared with the standard retinoic acid, but no irritant effect was observed for either essential oil suggesting their safety for topical application.

scholarly journals Antiangiogenic activity of zinc and zinc-sorafenib combination using the chick chorioallantoic membrane assay: a descriptive study

2017 ◽  
Vol 6 (5) ◽  
pp. 1060
Author(s):  
Manu Kumar ◽  
Girish Gulab Meshram ◽  
Tripti Rastogi ◽  
Sonal Sharma ◽  
Rachna Gupta ◽  
...  
Keyword(s):  
Kinase Inhibitor ◽  
Chorioallantoic Membrane ◽  
Angiogenesis Inhibitor ◽  
Control Group ◽  
Cam Assay ◽  
Dose Ratio ◽  
Antiangiogenic Activity ◽  
Chick Chorioallantoic Membrane ◽  
Branching Points ◽  
Chorioallantoic Membrane Assay

Background: Zinc, a trace element, is known for downregulating several proangiogenic growth factors and cytokines. However, its antiangiogenic activity is not adequately studied. The present study was aimed to evaluate the possible antiangiogenic activity of zinc via the chick chorioallantoic membrane (CAM) assay. Also, the antiangiogenic activity of the combination therapy of zinc with various doses of sorafenib, a tyrosine kinase inhibitor, was evaluated.Methods: A pilot study was initially conducted so as to select suitable doses of zinc and sorafenib. The antiangiogenic activity after combining zinc 2.5 μg/embryo with sorafenib 1, and 2 μg/embryo was also evaluated. The antiangiogenic activity was quantified in terms of total length of blood vessels, number of junctions, number of branching points, and mean length of the blood vessels.Results: Zinc 2.5 μg/embryo showed significant (p <0.05) antiangiogenic activity, as compared to the control group. However, its effect was not comparable to that of sorafenib 2 μg/embryo. The combination of zinc 2.5 μg/embryo with sorafenib 2 μg/embryo did not show an additive/synergistic effect. The combination of zinc 2.5 μg/embryo with sorafenib 1 μg/embryo produced an antiangiogenic activity which was comparable (p >0.05) to that of sorafenib 2 μg/embryo.Conclusions: Zinc caused significant antiangiogenic activity in the CAM assay. The lack of addition/synergism in the zinc-sorafenib combination could have been due to the variability in the dose/ratio selection. Addition of zinc to sorafenib therapy could improve treatment tolerability, reduce cost of therapy, and reduce the emergence of drug resistance. Future mechanistic studies could identify the exact pharmacodynamics of zinc as an angiogenesis inhibitor.

scholarly journals CONSISE SYNTHESIS AND POTENTIAL ANTI-ANGIOGENIC ACTIVITY OF N-1 SUBSTITUTED INDOLYLCHALCONE HYBRIDS ON CHORIOALLANTOIC MEMBRANE (CAM) ASSAY

2021 ◽  
pp. 81-86
Author(s):  
ANJALI M. WANEGAONKAR ◽  
MILIND J. BHITRE ◽  
DILLIP ZAVERI ◽  
SHRIRAM H. BAIRAGI
Keyword(s):  
Phase Transfer ◽  
Chorioallantoic Membrane ◽  
Assay Method ◽  
Morphometric Study ◽  
Significant Activity ◽  
Cam Assay ◽  
Potent Effect ◽  
Antiangiogenic Effect ◽  
Angiogenic Activity ◽  
Dose Dependent

Objective: Synthesis of N-1 Substituted Indolylchalcone Hybrids and evaluation of anti-angiogenic activity using Chorioallantoic Membrane (CAM) Assay. Methods: Claisen-schmidt reaction is used for the synthesis of 30 Indolylchalcone hybrids, it involves condensation of N-1 substituted indole-3-carboxaldehyde and N1 substituted 2-acetyl-benzimidazole. The phase transfer catalyst, a green catalyst such as anhydrous potassium carbonate (K2CO3) and PEG-400 are used in the alkylation and arylation. All synthesized indolylchalcone hybrids were evaluated for their antiangiogenic activity by in vivo-chorioallantoic membrane (CAM) assay method. Results: The synthesized indolylchalcone compounds are evaluated. The morphometric study was carried out as described by Melkonian et al. (2002). The Compounds with code C-2, I-1, I-2 are showing the more potent effect on the dose-dependent assay of CAM. The compounds with code C-1, C-3, E-1 to E-3, M-1 and M-5 shows the significant activity, however, though the compounds with code B-1, B-2, CL-1 and A-5 were showing antiangiogenic effect at 0.1 µM, but does not show any significant activity on dose-dependent assay of CAM. Conclusion: The synthesized Indolylchalcones as shown in the graph possess very good dose-dependent anti-angiogenic activities. The potency of anti-angiogenetic activity shows that methyl>Ethyl>Cl-benzyl>Benzyl>Isobutyl. 2-acetyl benzimidazole analogs have possible future scope to develop as potent angiogenesis inhibitors.

scholarly journals Heparinized Chitosan/hydroxyapatite Scaffolds Stimulate Angiogenesis in CAM Assay: Future Proangiogenic Materials to Promote Neovascularization to Accelerate Bone Regeneration

2020 ◽  
Author(s):  
Griselda V Nájera-Romero ◽  
Muhammad Yar ◽  
Ihtesham U Rehman
Keyword(s):  
Growth Factors ◽  
Bone Regeneration ◽  
Blood Vessels ◽  
Chorioallantoic Membrane ◽  
Cam Assay ◽  
Angiogenic Growth Factors ◽  
Healing Fracture ◽  
Angiogenic Effect ◽  
Synthesized Materials ◽  
Positive Effect

Abstract Formation of blood vessels during bone regeneration represents a major challenge for tissue engineered constructs. Poor revascularization can lead to scaffold failure and consequently, leads to non-healing fracture. Heparin is known to bind with angiogenic growth factors influencing the process of new blood vessels formation. There are several problems associated with the use of growth factors in clinic such as low stability, controlled delivery to the site, and price. The aim of the present study was to explore the potential of heparin to produce pro-angiogenic bone regeneration materials. Chitosan/hydroxyapatite freeze-gelled scaffolds were prepared and loaded with heparin. Different concentrations of heparin were successfully loaded onto the scaffolds, its release from the scaffold was analysed by toluidine blue assay and their angiogenic effect was evaluated by chorioallantoic membrane (CAM) assay to determine the optimal concentration of heparin to induce a proangiogenic effect. It was noted that low heparin concentrations exhibited a positive effect, with approximately 28 µg per scaffold indicating a significant increment in blood vessels. The synthesized materials showed no cytotoxic effects when evaluated by using U2OS cell line.

scholarly journals Sustainable Development of Chitosan/Calotropis procera-Based Hydrogels to Stimulate Formation of Granulation Tissue and Angiogenesis in Wound Healing Applications

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3284
Author(s):  
Muhammad Zahid ◽  
Maria Lodhi ◽  
Zulfiqar Ahmad Rehan ◽  
Hamna Tayyab ◽  
Talha Javed ◽  
...  
Keyword(s):  
Wound Healing ◽  
Blood Vessels ◽  
Granulation Tissue ◽  
Chorioallantoic Membrane ◽  
Calotropis Procera ◽  
Connective Tissues ◽  
Blood Capillaries ◽  
Chick Chorioallantoic Membrane ◽  
Thaw Cycle ◽  
Freeze Thaw Cycle

The formation of new scaffolds to enhance healing magnitude is necessarily required in biomedical applications. Granulation tissue formation is a crucial stage of wound healing in which granulation tissue grows on the surface of a wound by the formation of connective tissue and blood vessels. In the present study, porous hydrogels were synthesized using chitosan incorporating latex of the Calotropis procera plant by using a freeze–thaw cycle to stimulate the formation of granulation tissue and angiogenesis in wound healing applications. Structural analysis through Fourier transform infrared (FTIR) spectroscopy confirmed the interaction between chitosan and Calotropis procera. Latex extract containing hydrogel showed slightly higher absorption than the control during water absorption analysis. Thermogravimetric analysis showed high thermal stability of the 60:40 combination of chitosan (CS) and Calotropis procera as compared to all other treatments and controls. A fabricated scaffold application on a chick chorioallantoic membrane (CAM) showed that all hydrogels containing latex extract resulted in a significant formation of blood vessels and regeneration of cells. Overall, the formation of connective tissues and blood capillaries and healing magnitude decreased in ascending order of concentration of extract.

scholarly journals Pro-angiogenic and osteogenic composite scaffolds of fibrin, alginate and calcium phosphate for bone tissue engineering

2021 ◽  
Vol 12 ◽  
pp. 204173142110056
Author(s):  
Nupur Kohli ◽  
Vaibhav Sharma ◽  
Alodia Orera ◽  
Prasad Sawadkar ◽  
Nazanin Owji ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Calcium Phosphate ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Chorioallantoic Membrane ◽  
Cam Assay ◽  
Clinical Model ◽  
Chick Chorioallantoic Membrane ◽  
Biomimetic Method ◽  
Alginate Scaffold

Due to the limitations of bone autografts, we aimed to develop new composite biomaterials with pro-angiogenic and osteogenic properties to be used as scaffolds in bone tissue engineering applications. We used a porous, cross-linked and slowly biodegradable fibrin/alginate scaffold originally developed in our laboratory for wound healing, throughout which deposits of calcium phosphate (CaP) were evenly incorporated using an established biomimetic method. Material characterisation revealed the porous nature and confirmed the deposition of CaP precursor phases throughout the scaffolds. MC3T3-E1 cells adhered to the scaffolds, proliferated, migrated and differentiated down the osteogenic pathway during the culture period. Chick chorioallantoic membrane (CAM) assay results showed that the scaffolds were pro-angiogenic and biocompatible. The work presented here gave useful insights into the potential of these pro-angiogenic and osteogenic scaffolds for bone tissue engineering and merits further research in a pre-clinical model prior to its clinical translation.

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