Formation and Distribution of Sennosides in Cassia angustifolia, as Determined by a Sensitive and Specific Radioimmunoassay*

R. Atzorn; E. Weiler; M. Zenk

doi:10.1055/s-2007-971666

ASSESSMENT OF RADIOIODINATED HORMONE PREPARATIONS

Acta Endocrinologica ◽

10.1530/acta.0.062s134 ◽

1969 ◽

Vol 62 (1_Suppl) ◽

pp. S134-S144 ◽

Cited By ~ 4

Author(s):

W. M. Hunter

Keyword(s):

Immunological Reactivity ◽

Specific Radioimmunoassay ◽

Specific Activities

ABSTRACT The preparation of radioiodinated human LH and human FSH with specific activities of 50—150 μc/μg for use in specific radioimmunoassay systems is described. Methods for minimising iodination damage, for removing severely damaged fractions after iodination and for assessing the immunological reactivity of the final products are detailed. The obligation upon immunoassayists to demonstrate that their labelled preparations do indeed represent the hormones under consideration is discussed and criteria which may contribute evidence on this question are put forward.

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Detection and Quantification of 7-Hydroxydehydroepiandrosterone Epimers in Three Body Fluids

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc20020010 ◽

2002 ◽

Vol 67 (1) ◽

pp. 10-18 ◽

Cited By ~ 1

Author(s):

Richard Hampl ◽

Martin Hill ◽

Luboslav Stárka

Keyword(s):

Healthy Subjects ◽

Serum Levels ◽

Body Fluids ◽

Gas Chromatography Mass Spectrometry ◽

Specific Radioimmunoassay ◽

Order Of Magnitude ◽

Three Body ◽

First Time ◽

Nanomolar Range ◽

Detection And Quantification

3β,7α-Dihydroxyandrost-5-en-17-one (1) (7α-OH-DHEA) and its 7β-hydroxy epimer 2 (7β-OH-DHEA) - 7α- and 7β-hydroxydehydroepiandrosterone - were detected and quantified in three human body fluids: in blood serum, saliva and ejaculate. Specific radioimmunoassay and gas chromatography-mass spectrometry have been used. For the first time the data on changes of these dehydroepiandrosterone metabolites are reported for a representative group of healthy subjects of both sexes (172 females and 217 males) during the life span. The serum levels of both 7-hydroxydehydroepiandrosterone epimers in serum and also in semen were in the low nanomolar range, while concentrations by one order of magnitude lower were found in saliva, but still within the detection limit. The results will serve as a basis for comparative studies of 7-hydroxydehydroepiandrosterone levels under various pathophysiological conditions, with a particular respect to autoimmune disorders.

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Authentication of herbal drug Senna (Cassia angustifolia Vahl.): A village pharmacy for Indo-Pak Subcontinent

African Journal of Pharmacy and Pharmacology ◽

10.5897/ajpp12.446 ◽

2012 ◽

Vol 6 (30) ◽

Cited By ~ 2

Author(s):

Shazia Sultana

Keyword(s):

Herbal Drug ◽

Cassia Angustifolia

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Elucidation of zinc and copper induced oxidative stress, DNA damage and activation of defence system during seed germination in Cassia angustifolia Vahl

Environmental and Experimental Botany ◽

10.1016/j.envexpbot.2016.02.001 ◽

2016 ◽

Vol 125 ◽

pp. 31-41 ◽

Cited By ~ 38

Author(s):

Rajeshwari Nanda ◽

Veena Agrawal

Keyword(s):

Oxidative Stress ◽

Dna Damage ◽

Seed Germination ◽

Cassia Angustifolia ◽

Defence System

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Measurement of Leukotriene Release from Rat Peritoneal Cells with a Specific Radioimmunoassay

Annals of the New York Academy of Sciences ◽

10.1111/j.1749-6632.1984.tb13744.x ◽

1984 ◽

Vol 435 (1 First Colloqu) ◽

pp. 113-115

Author(s):

DAVID AHARONY ◽

PAUL DOBSON ◽

PETER BERNSTEIN ◽

ROBERT D. KRELL ◽

J. BRYAN SMITH

Keyword(s):

Peritoneal Cells ◽

Specific Radioimmunoassay

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Immunochemical analysis of cartilage proteoglycans. Radioimmunoassay of the molecules and the substructures

Biochemical Journal ◽

10.1042/bj1870687 ◽

1980 ◽

Vol 187 (3) ◽

pp. 687-694 ◽

Cited By ~ 19

Author(s):

J Wieslander ◽

D Heinegárd

Keyword(s):

Hyaluronic Acid ◽

Uronic Acid ◽

Chondroitin Sulphate ◽

Relative Content ◽

Binding Region ◽

Rich Region ◽

Link Protein ◽

Specific Radioimmunoassay ◽

Cartilage Proteoglycans ◽

Hyaluronic Acid Binding

Antibodies specifically reacting with the link proteins, the hyaluronic acid-binding region and chondroitin sulphate-peptides were used to design specific radioimmunoassay procedures. The sensitivity of the method used for the link protein was about 20 ng/ml, and the other two components could be determined at concentrations of about 2 ng/ml. The radioimmunoassay procedures were tested by using proteoglycan subfractions or fragments thereof. The procedures used to quantify link protein and hyaluronic acid-binding region showed no cross-interference. Fragments of trypsin-digested proteoglycan monomers still reacted in the radioimmunoassay for hyaluronic acid-binding region. Subfractions of proteoglycan monomers separated according to size had a gradually higher relative content of the hyaluronic acid-binding region compared with both chondroitin sulphate-peptides and uronic acid, when the molecules were smaller. The proteoglycans therefore may contain a variably large chondroitin sulphate-rich region, which has a constant substitution with polysaccharide side chains.

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