Short- and long-term cytotoxic effects of doxorubicin conjugates with dendrimers and vector protein on MCF-7/MDR1 chemoresistant breast cancer cells

Paclitaxel is an effective chemotherapeutic agent that is widely used for the treatment of several cancers, including breast, ovarian, and non-small-cell lung cancer. Due to its high lipophilicity, paclitaxel is difficult to administer and requires solubilization with Cremophor EL (polyethoxylated castor oil) and ethanol, which often lead to adverse side effects, including life-threatening anaphylaxis. Incorporation of paclitaxel in dimyristoylphosphatidylcholine:dimyristoylphosphatidylglycerol (DPPC:DMPG) liposomes can facilitate its delivery to cancer cells and eliminate the adverse reactions associated with the Cremophor EL vehicle. Accordingly, the effectiveness of liposomal paclitaxel on MCF-7 breast cancer cells was examined. The results from this study showed that (i) the lipid components of the liposomal formulation were nontoxic, (ii) the cytotoxic effects of liposomal paclitaxel were improved when compared with those seen with conventional paclitaxel, and (iii) the intracellular paclitaxel levels were higher in MCF-7 cells treated with the liposomal paclitaxel formulation. The results of these studies showed that delivery of paclitaxel as a liposomal formulation could be a promising strategy for enhancing its chemotherapeutic effects.

Download Full-text

The Study of Cytotoxic Effects of Boldine Derivatives on Human Breast Cancer Cells- MCF-7 and MDA-MB231 Cell Lines

Multidisciplinary Cancer Investigation ◽

10.21859/mci-supp-01 ◽

2017 ◽

Vol 1 (Supplementary 1) ◽

pp. 0-0

Author(s):

Younes Zarei ◽

Sakineh Kazemi Noureini

Keyword(s):

Breast Cancer ◽

Cancer Cells ◽

Cell Lines ◽

Human Breast Cancer ◽

Breast Cancer Cells ◽

Human Breast Cancer Cells ◽

Human Breast ◽

Cytotoxic Effects ◽

Mb231 Cell ◽

Mcf 7

Download Full-text

Short- and long-term estrogen deprivation of T47D human breast cancer cells in culture

European Journal of Cancer and Clinical Oncology ◽

10.1016/0277-5379(89)90348-9 ◽

1989 ◽

Vol 25 (12) ◽

pp. 1777-1788 ◽

Cited By ~ 24

Author(s):

Catherine S. Murphy ◽

Lorraine F. Meisner ◽

Shi Qi Wu ◽

V. Craig Jordan

Keyword(s):

Breast Cancer ◽

Cancer Cells ◽

Human Breast Cancer ◽

Breast Cancer Cells ◽

Human Breast Cancer Cells ◽

Human Breast ◽

Estrogen Deprivation ◽

Cells In Culture ◽

Short And Long Term

Download Full-text

CURCUMIN INCREASES THE SENSITIVITY OF BREAST CANCER CELLS TO TAMOXIFEN BY INHIBITING MRP2 MRNA EXPRESSION OF EFFLUX TRANSPORTER MRP2

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2019.v11s6.33553 ◽

2019 ◽

pp. 88-90

Author(s):

DESAK GEDE BUDI KRISNAMURTI ◽

SEPTELIA INAWATI WANANDI ◽

MELVA LOUISA

Keyword(s):

Breast Cancer ◽

T Cells ◽

Cell Viability ◽

Mrna Expression ◽

Cancer Cells ◽

Breast Cancer Cells ◽

Tamoxifen Treatment ◽

Efflux Transporter ◽

Mcf 7

Objective: Tamoxifen is the drug of choice to treat breast cancer positive for estrogen receptor. Long-term use of tamoxifen can induce multidrug resistance (MDR) associated with decreased sensitivity of cancer cells to the drug. One of the causes of MDR is overexpression of efflux transporter multidrug resistance-associated protein (MRP)2. Various drugs are known to act as MRP2 inhibitors, including curcumin. This study investigated the effects of curcumin on the sensitivity of breast cancer cells to tamoxifen through inhibition of MRP2. Methods: We used MCF-7 cells that were previously exposed to long-term tamoxifen treatment [MCF-7(T) cells]. MCF-7(T) cells were treated with 1 µM tamoxifen, curcumin (5, 10, and 20 µM), combinations of curcumin (5, 10, and 20 µM) and 1 µM tamoxifen, or 10 µM nevirapine (a known MRP2 inhibitor) for 5 d. Then, the cells were harvested, counted to assess cell viability, and evaluated for MRP2 mRNA expression. Results: Treatment with curcumin alone or in combination with tamoxifen significantly reduced cell viability at all curcumin concentrations compared with the control. The reduction in cell viability was accompanied by a reduced level of MRP2 mRNA expression. Conclusion: Application of curcumin to MCF-7 cells previously exposed to long-term tamoxifen treatment increase the sensitivity of cancer cells to tamoxifen. The increased sensitivity of these cells was attributed, at least in part, to inhibition of the efflux transporter MRP2.

Download Full-text