Theoretical analysis of bimetallic nanorod dimer biosensors for label-free molecule detection

Knudsen force acting on a heated microbeam adjacent to a cold substrate in a rarefied gas is a mechanical force created by unbalanced thermal gradients. The measured force has its direction pointing towards the side with a lower thermal gradient and its magnitude vanishes in both continuum and free-molecule limits. In our previous study, negative Knudsen forces were discovered at the high Knudsen regime before diminishing in the free-molecule limit. Such a phenomenon was however not observed in the experiment. In this paper, the existence of such a negative Knudsen force is further confirmed using both numerical simulation and theoretical analysis. The asymptotic order of the Knudsen force near the collisionless limit is analyzed and the analytical expression of its leading term is provided, from which approaches for the enhancement of negative Knudsen forces are proposed.

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Graph Theoretical Analysis, Insilico Modeling and Formulation of Pyrimidine Nanoparticles as p38α MAP Kinases inhibitors: A Quantitative Proteomics Approach

Drug Research ◽

10.1055/a-0650-3979 ◽

2018 ◽

Vol 69 (02) ◽

pp. 100-110 ◽

Cited By ~ 2

Author(s):

Panneerselvam Theivendren ◽

Selvaraj Kunjiappan ◽

Saravanan Govindraj ◽

Jaikanth Chandrasekarn ◽

Parasuraman Pavadai ◽

...

Keyword(s):

Theoretical Analysis ◽

Anticancer Activity ◽

Quantitative Proteomics ◽

Density Functional ◽

Map Kinases ◽

Label Free ◽

Proteomics Data ◽

Experimental Conditions ◽

Regulatory Pathways ◽

Graph Theoretical Analysis

AbstractIn this study, the optimized 4-(4-hydroxybenzyl)-2-amino-6-hydroxypyrimidine-5-carboxamide derivative was formulated as nanoparticles to evaluate for their anticancer activity. The response surface methodology (RSM) was performed with utilization of Box-Behnken statistical design (BBSD) to optimize the experimental conditions for identification of significant synthetic methodology. To explore the stability of the derivative was done by density functional theory (DFT). Graph theoretical analysis was introduced to identify the drug target p38α MAP Kinases and then insilico modeling was performed to provide straightforward information for further structural optimization. The experimental results under optimal experimental conditions obtained 74.55–76% yield of 4-(4-hydroxybenzyl)-2-amino-6-hydroxypyrimidine-5-carboxamide, 127oC melting point and Rf value 0.59 were well matched with the predicted results and this was gaining 95% of confidence level and suitability of RSM. The spectral data were reliable with the assigned structures of synthetic yields. The formulated nanoparticles were exhibited a good anticancer activity against used cancer cell line MCF7. Amusingly the observed docking scores and in-vitro anticancer activity was proving the compound significance and potential as a potent p38α inhibitor. Further, we have elucidated the mechanism of action at its functional level using label-free quantitative proteomics. Interestingly the observed results were indicating that the derived proteomics data involving in the alteration process in cancer-related regulatory pathways.

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Microdiffraction Patterns of Small Metallic Particles II; Theoretical Analysis

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100055680 ◽

1982 ◽

Vol 40 ◽

pp. 668-669

Author(s):

A. Gómez ◽

P. Schabes-Retchkiman ◽

M. José-Yacamán ◽

T. Ocaña

Keyword(s):

Experimental Data ◽

Electron Diffraction ◽

Theoretical Analysis ◽

Size Range ◽

Dynamical Theory ◽

Metallic Particles ◽

Splitting Effect

The splitting effect that is observed in microdiffraction pat-terns of small metallic particles in the size range 50-500 Å can be understood using the dynamical theory of electron diffraction for the case of a crystal containing a finite wedge. For the experimental data we refer to part I of this work in these proceedings.

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Label-free, mass-sensitive single-molecule imaging using interferometric scattering microscopy

Essays in Biochemistry ◽

10.1042/ebc20200023 ◽

2020 ◽

Author(s):

Nikolas Hundt

Keyword(s):

Single Molecule ◽

Cellular Systems ◽

Single Molecule Imaging ◽

Label Free ◽

Measurement Sensitivity ◽

Mass Distributions ◽

Quantitative Measurements ◽

Contrast Mechanism ◽

Cellular Components ◽

Scattering Signal

Abstract Single-molecule imaging has mostly been restricted to the use of fluorescence labelling as a contrast mechanism due to its superior ability to visualise molecules of interest on top of an overwhelming background of other molecules. Recently, interferometric scattering (iSCAT) microscopy has demonstrated the detection and imaging of single biomolecules based on light scattering without the need for fluorescent labels. Significant improvements in measurement sensitivity combined with a dependence of scattering signal on object size have led to the development of mass photometry, a technique that measures the mass of individual molecules and thereby determines mass distributions of biomolecule samples in solution. The experimental simplicity of mass photometry makes it a powerful tool to analyse biomolecular equilibria quantitatively with low sample consumption within minutes. When used for label-free imaging of reconstituted or cellular systems, the strict size-dependence of the iSCAT signal enables quantitative measurements of processes at size scales reaching from single-molecule observations during complex assembly up to mesoscopic dynamics of cellular components and extracellular protrusions. In this review, I would like to introduce the principles of this emerging imaging technology and discuss examples that show how mass-sensitive iSCAT can be used as a strong complement to other routine techniques in biochemistry.

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Theoretical analysis of a monopole antenna with conducting flat disk above the earth

Electronics and Communications in Japan (Part I Communications) ◽

10.1002/ecja.1021.abs ◽

2001 ◽

Vol 84 (7) ◽

pp. 27-36

Author(s):

Aki Yuasa ◽

Daisuke Itatsu ◽

Naoki Inagaki ◽

Nobuyoshi Kikuma

Keyword(s):

Theoretical Analysis ◽

Monopole Antenna ◽

The Earth ◽

Flat Disk

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Context Aversion, Pavlovian Conditioning, and the Psychological Side Effects of Chemotherapy

European Psychologist ◽

10.1027/1016-9040.2.118 ◽

1997 ◽

Vol 2 (2) ◽

pp. 118-124

Author(s):

Geoffrey Hall

Keyword(s):

Animal Model ◽

Side Effects ◽

Theoretical Analysis ◽

Conditioned Response ◽

Behavioral Strategies ◽

Contextual Cues ◽

Anticipatory Nausea ◽

Laboratory Rats ◽

Anticipatory Nausea And Vomiting ◽

Parallel Case

Patients who have undergone several sessions of chemotherapy for cancer will sometimes develop anticipatory nausea and vomiting (ANV), these unpleasant side effects occurring as the patients return to the clinic for a further session of treatment. Pavlov's analysis of learning allows that previously neutral cues, such as those that characterize a given place or context, can become associated with events that occur in that context. ANV could thus constitute an example of a conditioned response elicited by the contextual cues of the clinic. In order to investigate this proposal we have begun an experimental analysis of a parallel case in which laboratory rats are given a nausea-inducing treatment in a novel context. We have developed a robust procedure for assessing the acquisition of context aversion in rats given such training, a procedure that shows promise as a possible animal model of ANV. Theoretical analysis of the conditioning processes involved in the formation of context aversions in animals suggests possible behavioral strategies that might be used in the alleviation of ANV, and we report a preliminary experimental test of one of these.

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