scholarly journals Isolation, identification and bioactivity of steroids compounds from red algae Eucheuma cottonii petroleum ether fraction

2019 ◽  
Author(s):  
Ahmad Ghanaim Fasya ◽  
Ahmad Baderos ◽  
Armeida Dwi Ridhowati Madjid ◽  
Suci Amalia ◽  
Dewi Sinta Megawati
Keyword(s):  
Petroleum Ether ◽  
Red Algae ◽  
Petroleum Ether Fraction ◽  
Ether Fraction ◽  
Eucheuma Cottonii

scholarly journals Variasi Komposisi Eluen pada Isolasi Steroid dan Triterpenoid Alga Merah Eucheuma cottonii dengan Kromatografi Kolom Basah

ALCHEMY ◽  
2020 ◽  
Vol 8 (1) ◽  
pp. 35-40
Author(s):  
Armeida Dwi Ridhowati Madjid ◽  
Dwi Anik Rahmawati ◽  
Ahmad Ghanaim Fasya
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Functional Groups ◽  
Column Chromatography ◽  
Methanol Extract ◽  
Eluent Composition ◽  
Petroleum Ether Fraction ◽  
Ether Fraction ◽  
Layer Chromatography ◽  
Eucheuma Cottonii

Steroid and triterpenoid compounds in petroleum ether fractions of red algae Eucheuma cottonii were isolated by slurry column chromatography. Isolation was started with maceration extraction using methanol solvent. The methanol extract was hydrolyzed with HCl 2 N and partitioned using petroleum ether solvent. The presence of steroid and triterpenoid was determined by phytochemical test using Lieberman Burchard reagent. Petroleum ether fraction was separated by slurry column chromatography with the variation of eluent composition n-hexane: ethyl acetate 16:4; 17:3; 18:2. The isolates were monitored by analytical thin-layer chromatography (TLC) and the best result was identified using FTIR. Percent yields of methanol extract and petroleum ether fraction were 11.866% and 8.03%, respectively. The optimum eluent composition in column chromatography for separation was n-hexane : ethyl acetate (18:2) obtained 2 steroid and 3 triterpenoid fractions. FTIR analysis gives information about functional groups of –OH, C=C, C-O, CH2, -C(CH3)2forsteroids and functional groups of –OH, C=O, C=C, CH2, -C(CH3)2 for triterpenoid.Keywords: Eucheuma cottonii, column chromatography, steroid, triterpenoid, variation of eluent composition  Senyawa steroid dan triterpenoid dalam fraksi petroleum eter alga merah Eucheuma cottonii telah diisolasi menggunakan kromatografi kolom basah. Isolasi dilakukan dengan cara ekstraksi maserasi menggunakan pelarut metanol. Ekstrak pekat metanol dihidrolisis dengan HCl 2 N dan dipartisi menggunakan pelarut petroleum eter. Senyawa steroid dan triterpenoid diuji fitokimia menggunakan reagen Lieberman Burchard. Fraksi petroleum eter dipisahkan menggunakan kromatografi kolom basah variasi komposisi eluen n-heksana:etil asetat dengan perbandingan 16:4; 17:3; dan 18:2. Hasil isolasi dimonitoring menggunakan kromatografi lapis tipis (KLT) analitik. Hasil monitoring yang terbaik diidentifikasi gugus fungsinya menggunakan FTIR. Hasil penelitian menunjukkan rendemen ekstrak metanol dan fraksi petroleum eter masing-masing sebesar 11,866% dan 8,03%. Pemisahan kolom dengan variasi komposisi eluen n-heksana:etil asetat terbaik adalah 18:2 dengan diperoleh 2 kelompok fraksi steroid dan 3 kelompok fraksi triterpenoid. Hasil analisis FTIR pada isolat steroid memberikan informasi gugus –OH, C=C, C-O, CH2, -C(CH3)2, sedangkan isolat triterpenoid memberikan informasi gugus –OH, C=O, C=C, CH2,-C(CH3)2. Gugus gem dimetil (-C(CH3)2)merupakan ciri khas rantai samping steroid ataupun triterpenoid.Kata kunci: Eucheuma cottonii, kromatografi kolom, steroid, triterpenoid, variasi komposisi eluen

The Suppressive Effects of the Petroleum Ether Fraction fromAtractylodes lancea(Thunb.) DC. On a Collagen-Induced Arthritis Model

2016 ◽  
Vol 30 (10) ◽  
pp. 1672-1679 ◽  
Author(s):  
Renping Liu ◽  
Enwei Tao ◽  
Shuwen Yu ◽  
Bo Liu ◽  
Lingman Dai ◽  
...  
Keyword(s):  
Petroleum Ether ◽  
Collagen Induced Arthritis ◽  
Arthritis Model ◽  
Petroleum Ether Fraction ◽  
Ether Fraction

scholarly journals Isolation and bioactivity of steroids isolates from petroleum ether fraction of Chlorella sp.

2020 ◽  
Author(s):  
A. G. Fasya ◽  
N. Millati ◽  
L. M. Rahmawati ◽  
R. Iyani ◽  
A. Hanapi ◽  
...  
Keyword(s):  
Petroleum Ether ◽  
Petroleum Ether Fraction ◽  
Chlorella Sp ◽  
Ether Fraction

scholarly journals Cytotoxic and micronuclei inducing effects of petroleum ether fraction of leaf aqueous extract of Clerodendrum viscosum Vent. in Allium cepa root tip cells

2021 ◽  
Author(s):  
Sujit Roy ◽  
Lalit Mohan Kundu ◽  
Gobinda Chandra Roy ◽  
Manabendu Barman ◽  
Sanjib Ray
Keyword(s):  
Petroleum Ether ◽  
Allium Cepa ◽  
Aqueous Extract ◽  
Root Tip ◽  
Petroleum Ether Fraction ◽  
Leaf Aqueous Extract ◽  
Ether Fraction ◽  
Clerodendrum Viscosum ◽  
Tip Cells ◽  
Root Tip Cells

Clerodendrum viscosum is a traditionally used medicinal plant. The present study aimed to analyze a detailed cytotoxic effect of the nonpolar petroleum ether fraction (AQPEF) of leaf aqueous extract of C. viscosum Vent. (LAECV) in Allium cepa root tip cells. The LAECV was fractionated with petroleum ether and tested for A. cepa toxicity at early hours (2 and 4 h treatment) at concentrations 0, 0.050, 0.100, 0.150 and 0.200 mg mL-1. The highest aberrant cell percentage (10.45%) was scored from 0.150 mg mL-1 followed by0.100 mg mL-1 (8.75%) concentration at 4 h treated samples. The AQPEF treatment induced a significant (p<0.0001) increase in micronuclei frequency; 4.31, 5.08, 5.05 and 3.05% respectively at concentrations 0.50, 0.100, 0.150, and 0.200 mg mL-1. The highest polyploid frequency (20.14%) induced with 0.100 mg mL-1 of AQPEF at 16 h recovery. 0.150 mg mL-1 is the most effective concentration of AQPEF to decipher its activity similar to colchicine (0.150 mg mL-1). In summary, the present study indicates petroleum ether is suitable for extraction of the active phytochemicals of LAECV having cytotoxic effects on A. cepa root tip cells. The AQPEF has colchicine like micronuclei, polyploidy, and mitotic abnormality inducing potentials in A. cepa root apical meristem cells.

scholarly journals Antinociceptive Activity of Petroleum Ether Fraction of Clinacanthus nutans Leaves Methanolic Extract: Roles of Nonopioid Pain Modulatory Systems and Potassium Channels

2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Zainul Amiruddin Zakaria ◽  
Mohammad Hafiz Abdul Rahim ◽  
Rushduddin Al Jufri Roosli ◽  
Mohd Hijaz Mohd Sani ◽  
Najihah Hanisah Marmaya ◽  
...  
Keyword(s):  
Petroleum Ether ◽  
Methanolic Extract ◽  
Antinociceptive Activity ◽  
Channel Blockers ◽  
Tetraethylammonium Chloride ◽  
Petroleum Ether Fraction ◽  
Ether Fraction ◽  
Clinacanthus Nutans ◽  
Antinociceptive Mechanisms ◽  
Derivatives Of

Methanolic extract of Clinacanthus nutans Lindau leaves (MECN) has been reported to exert antinociceptive activity. The present study aimed to elucidate the possible antinociceptive mechanisms of a lipid-soluble fraction of MECN, which was obtained after sequential extraction in petroleum ether. The petroleum ether fraction of C. nutans (PECN), administered orally to mice, was (i) subjected to capsaicin-, glutamate-, phorbol 12-myristate 13-acetate-, bradykinin-induced nociception model; (ii) prechallenged (intraperitoneal (i.p.)) with 0.15 mg/kg yohimbine, 1 mg/kg pindolol, 3 mg/kg caffeine, 0.2 mg/kg haloperidol, or 10 mg/kg atropine, which were the respective antagonist of α2-adrenergic, β-adrenergic, adenosinergic, dopaminergic, or muscarinic receptors; and (iii) prechallenged (i.p.) with 10 mg/kg glibenclamide, 0.04 mg/kg apamin, 0.02 mg/kg charybdotoxin, or 4 mg/kg tetraethylammonium chloride, which were the respective inhibitor of ATP sensitive-, small conductance Ca2+-activated-, large conductance Ca2+-activated-, or nonselective voltage-activated-K+ channel. Results obtained demonstrated that PECN (100, 250, and 500 mg/kg) significantly (P<0.05) inhibited all models of nociception described earlier. The antinociceptive activity of 500 mg/kg PECN was significantly (P<0.05) attenuated when prechallenged with all antagonists or K+ channel blockers. However, only pretreatment with apamin and charybdotoxin caused full inhibition of PECN-induced antinociception. The rest of the K+ channel blockers and all antagonists caused only partial inhibition of PECN antinociception, respectively. Analyses on PECN’s phytoconstituents revealed the presence of antinociceptive-bearing bioactive compounds of volatile (i.e., derivatives of γ–tocopherol, α–tocopherol, and lupeol) and nonvolatile (i.e., cinnamic acid) nature. In conclusion, PECN exerts a non-opioid-mediated antinociceptive activity involving mainly activation of adenosinergic and cholinergic receptors or small- and large-conductance Ca2+-activated-K+ channels.

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