scholarly journals GC-MS and FT-IR Analyses of Phytocomponents From petroleum ether Fraction of Leaf Extract of Psidium guajava

2019 ◽  
Vol 13 (1) ◽  
pp. 26-31
Author(s):  
Paul C. Chikezie ◽  
Raphael C. Ekeanyanwu ◽  
Adaeze B. Chile-Agad
Author(s):  
Sruthi Radhakrishnan

Green route for the synthesis of nanoparticles has become more acceptable than the other chemical as well as biological route. In the present study, silver nanoparticle is synthesized using ethanolic extract of Psidium guajava leaves. Further the synthesized silver nanoparticles were characterized by UV-Visible Spec, FT-IR, X-Ray Diffraction FESEM and E-DAX. The results of FT-IR provided evidence of the involvement of phytochemicals present in the leaf extract in the reduction of silver nitrate to silver nanoparticles. XRD confirmed the crystalline structure as well as shape of the synthesized nanoparticle as face-centred cubic. E-DAX profiling helped in determining the presence of elemental silver. The size of the nanoparticle procured by SEM analysis was found to be approximately 30-50 nm in size. Thus, the findings of this study showed that the plant assisted method for silver nanoparticle synthesis is more effective and further application level studies can shed lights on their use in healing of various human ailments.   


2016 ◽  
Vol 30 (10) ◽  
pp. 1672-1679 ◽  
Author(s):  
Renping Liu ◽  
Enwei Tao ◽  
Shuwen Yu ◽  
Bo Liu ◽  
Lingman Dai ◽  
...  

2020 ◽  
Author(s):  
A. G. Fasya ◽  
N. Millati ◽  
L. M. Rahmawati ◽  
R. Iyani ◽  
A. Hanapi ◽  
...  

2021 ◽  
Author(s):  
Sujit Roy ◽  
Lalit Mohan Kundu ◽  
Gobinda Chandra Roy ◽  
Manabendu Barman ◽  
Sanjib Ray

Clerodendrum viscosum is a traditionally used medicinal plant. The present study aimed to analyze a detailed cytotoxic effect of the nonpolar petroleum ether fraction (AQPEF) of leaf aqueous extract of C. viscosum Vent. (LAECV) in Allium cepa root tip cells. The LAECV was fractionated with petroleum ether and tested for A. cepa toxicity at early hours (2 and 4 h treatment) at concentrations 0, 0.050, 0.100, 0.150 and 0.200 mg mL-1. The highest aberrant cell percentage (10.45%) was scored from 0.150 mg mL-1 followed by0.100 mg mL-1 (8.75%) concentration at 4 h treated samples. The AQPEF treatment induced a significant (p<0.0001) increase in micronuclei frequency; 4.31, 5.08, 5.05 and 3.05% respectively at concentrations 0.50, 0.100, 0.150, and 0.200 mg mL-1. The highest polyploid frequency (20.14%) induced with 0.100 mg mL-1 of AQPEF at 16 h recovery. 0.150 mg mL-1 is the most effective concentration of AQPEF to decipher its activity similar to colchicine (0.150 mg mL-1). In summary, the present study indicates petroleum ether is suitable for extraction of the active phytochemicals of LAECV having cytotoxic effects on A. cepa root tip cells. The AQPEF has colchicine like micronuclei, polyploidy, and mitotic abnormality inducing potentials in A. cepa root apical meristem cells.


2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Zainul Amiruddin Zakaria ◽  
Mohammad Hafiz Abdul Rahim ◽  
Rushduddin Al Jufri Roosli ◽  
Mohd Hijaz Mohd Sani ◽  
Najihah Hanisah Marmaya ◽  
...  

Methanolic extract of Clinacanthus nutans Lindau leaves (MECN) has been reported to exert antinociceptive activity. The present study aimed to elucidate the possible antinociceptive mechanisms of a lipid-soluble fraction of MECN, which was obtained after sequential extraction in petroleum ether. The petroleum ether fraction of C. nutans (PECN), administered orally to mice, was (i) subjected to capsaicin-, glutamate-, phorbol 12-myristate 13-acetate-, bradykinin-induced nociception model; (ii) prechallenged (intraperitoneal (i.p.)) with 0.15 mg/kg yohimbine, 1 mg/kg pindolol, 3 mg/kg caffeine, 0.2 mg/kg haloperidol, or 10 mg/kg atropine, which were the respective antagonist of α2-adrenergic, β-adrenergic, adenosinergic, dopaminergic, or muscarinic receptors; and (iii) prechallenged (i.p.) with 10 mg/kg glibenclamide, 0.04 mg/kg apamin, 0.02 mg/kg charybdotoxin, or 4 mg/kg tetraethylammonium chloride, which were the respective inhibitor of ATP sensitive-, small conductance Ca2+-activated-, large conductance Ca2+-activated-, or nonselective voltage-activated-K+ channel. Results obtained demonstrated that PECN (100, 250, and 500 mg/kg) significantly (P<0.05) inhibited all models of nociception described earlier. The antinociceptive activity of 500 mg/kg PECN was significantly (P<0.05) attenuated when prechallenged with all antagonists or K+ channel blockers. However, only pretreatment with apamin and charybdotoxin caused full inhibition of PECN-induced antinociception. The rest of the K+ channel blockers and all antagonists caused only partial inhibition of PECN antinociception, respectively. Analyses on PECN’s phytoconstituents revealed the presence of antinociceptive-bearing bioactive compounds of volatile (i.e., derivatives of γ–tocopherol, α–tocopherol, and lupeol) and nonvolatile (i.e., cinnamic acid) nature. In conclusion, PECN exerts a non-opioid-mediated antinociceptive activity involving mainly activation of adenosinergic and cholinergic receptors or small- and large-conductance Ca2+-activated-K+ channels.


Author(s):  
M.S. Sindhu ◽  
Poonkothai M.

The present study is to evaluate the phytochemical constituents and antimicrobial activity of Plectranthus amboinicus leaf extract using petroleum ether, methanol and water as solvents. The antimicrobial activity of P. amboinicus leaf extracts were examined against the selected bacterial and fungal isolates namely Escherichia coli, Staphylococcus aureus and Aspergillus niger, Candida albicans using agar well diffusion method. FT –IR analysis was performed to identify the presence of functional groups in the methanol extract. The results of the study revealed the presence of distinctive active secondary metabolites in the tested leaf extract. The results revealed that the methanol extract exhibited maximum antimicrobial activity against the tested bacterial and fungal isolates when compared with aqueous and petroleum ether extracts. The FT-IR analysis reveals the presence of functional groups such as hydroxyl, amines, alkynes, ketones and carboxylic acid in the methanolic extract of P. amboinicus. Thus, P. amboinicus leaves are effective against the selected microbes and recommends that the plant derived phytochemicals are comparatively safer than synthetic alternative, thereby contributing insightful remedial benefits for the treatment of diseases.


Sign in / Sign up

Export Citation Format

Share Document