scholarly journals Variasi Komposisi Eluen pada Isolasi Steroid dan Triterpenoid Alga Merah Eucheuma cottonii dengan Kromatografi Kolom Basah

ALCHEMY ◽  
2020 ◽  
Vol 8 (1) ◽  
pp. 35-40
Author(s):  
Armeida Dwi Ridhowati Madjid ◽  
Dwi Anik Rahmawati ◽  
Ahmad Ghanaim Fasya
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Functional Groups ◽  
Column Chromatography ◽  
Methanol Extract ◽  
Eluent Composition ◽  
Petroleum Ether Fraction ◽  
Ether Fraction ◽  
Layer Chromatography ◽  
Eucheuma Cottonii

Steroid and triterpenoid compounds in petroleum ether fractions of red algae Eucheuma cottonii were isolated by slurry column chromatography. Isolation was started with maceration extraction using methanol solvent. The methanol extract was hydrolyzed with HCl 2 N and partitioned using petroleum ether solvent. The presence of steroid and triterpenoid was determined by phytochemical test using Lieberman Burchard reagent. Petroleum ether fraction was separated by slurry column chromatography with the variation of eluent composition n-hexane: ethyl acetate 16:4; 17:3; 18:2. The isolates were monitored by analytical thin-layer chromatography (TLC) and the best result was identified using FTIR. Percent yields of methanol extract and petroleum ether fraction were 11.866% and 8.03%, respectively. The optimum eluent composition in column chromatography for separation was n-hexane : ethyl acetate (18:2) obtained 2 steroid and 3 triterpenoid fractions. FTIR analysis gives information about functional groups of –OH, C=C, C-O, CH2, -C(CH3)2forsteroids and functional groups of –OH, C=O, C=C, CH2, -C(CH3)2 for triterpenoid.Keywords: Eucheuma cottonii, column chromatography, steroid, triterpenoid, variation of eluent composition  Senyawa steroid dan triterpenoid dalam fraksi petroleum eter alga merah Eucheuma cottonii telah diisolasi menggunakan kromatografi kolom basah. Isolasi dilakukan dengan cara ekstraksi maserasi menggunakan pelarut metanol. Ekstrak pekat metanol dihidrolisis dengan HCl 2 N dan dipartisi menggunakan pelarut petroleum eter. Senyawa steroid dan triterpenoid diuji fitokimia menggunakan reagen Lieberman Burchard. Fraksi petroleum eter dipisahkan menggunakan kromatografi kolom basah variasi komposisi eluen n-heksana:etil asetat dengan perbandingan 16:4; 17:3; dan 18:2. Hasil isolasi dimonitoring menggunakan kromatografi lapis tipis (KLT) analitik. Hasil monitoring yang terbaik diidentifikasi gugus fungsinya menggunakan FTIR. Hasil penelitian menunjukkan rendemen ekstrak metanol dan fraksi petroleum eter masing-masing sebesar 11,866% dan 8,03%. Pemisahan kolom dengan variasi komposisi eluen n-heksana:etil asetat terbaik adalah 18:2 dengan diperoleh 2 kelompok fraksi steroid dan 3 kelompok fraksi triterpenoid. Hasil analisis FTIR pada isolat steroid memberikan informasi gugus –OH, C=C, C-O, CH2, -C(CH3)2, sedangkan isolat triterpenoid memberikan informasi gugus –OH, C=O, C=C, CH2,-C(CH3)2. Gugus gem dimetil (-C(CH3)2)merupakan ciri khas rantai samping steroid ataupun triterpenoid.Kata kunci: Eucheuma cottonii, kromatografi kolom, steroid, triterpenoid, variasi komposisi eluen

scholarly journals Antimicrobial Activity of Akanda (Calotropis gigantea L.) on Some Pathogenic Bacteria.

1970 ◽  
Vol 43 (3) ◽  
pp. 397-404 ◽  
Author(s):  
M Ashraful Alam ◽  
M Rowshanul Habib ◽  
Rarjana Nikkon ◽  
Matiar Rahman ◽  
M Rezaul Karim
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Methanol Extract ◽  
Ethyl Acetate Fraction ◽  
Chloroform Fraction ◽  
Root Bark ◽  
Calotropis Gigantea ◽  
Petroleum Ether Fraction ◽  
Ether Fraction

The antibacterial activity of methanol extract from the root bark of Akanda (Calotropis gigantea L.) and its petroleum ether, chloroform and ethyl acetate fractions were investigated. Both of methanol extract and its chloroform fraction showed activity against Sarcina lutea, Bacillus megaterium and Pseudomonas aeruginosa. Petroleum ether fraction showed activity against Bacillus subtilis and Shigella sonnei whereas ethyl acetate fraction showed activity against Pseudomonas aeruginosa and Escherichia coli at 20ìg/disc, 30ìg/disc and 40ìg/disc doses. Among the tested materials, methanol extract and its chloroform fraction showed comparatively better results. Minimum inhibitory concentration (MIC) for methanol extract and each fraction were also determined by serial dilution technique. Keywords: Methanol extract, Akonda (Calotropis gigantea), Petroleum ether fraction, Chloroform fraction and Ethyl acetate fraction.   DOI = 10.3329/bjsir.v43i3.1156Bangladesh J. Sci. Ind. Res. 43(3), 397-404, 2008

scholarly journals ANALISIS FITOKIMIA DAN UJI EFEK SEDATIF DARI EKSTRAK ETANOL DAN BEBERAPA FRAKSI DAGING BUAH PALA (Myristica Fragrans Houtt)

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Eresia Makanaung ◽  
Johnly A Rorong ◽  
Edi Suryanto
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Ethanol Extract ◽  
Sedative Effect ◽  
Test Method ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Petroleum Ether Fraction ◽  
Ether Fraction ◽  
Traction Test

ABSTRAKPala adalah tanaman yang termasuk dalam jenis tanaman obat. Penelitian ini bertujuan untuk menganalisis total kandungan fitokimia dan uji efek sedatif  dari ekstrak etanol, fraksi petroleum eter, etil asetat dan air daging buah pala. Ekstraksi dilakukan dengan cara maserasi, kemudian ekstrak etanol yang diperoleh difraksinasi dengan cara partisi. Analisis fitokimia dilakukan dengan metode spektrofotometri UV-Vis sehingga diperoleh hasil kandungan total fenolik ekstrak etanol, fraksi petroleum eter, etil asetat dan air daging buah pala secara berturut-turut adalah 26,284 μg/mL; 29,203 μg/mL; 28,725 μg/mL; 24,904 μg/mL. Total flavonoid secara berturut-turut adalah 9,999 μg/mL; 13,095 μg/mL; 13,729 μg/mL; 10,475 μg/mL. Total tanin secara berturut-turut adalah 16,642 μg/mL; 16,571 μg/mL; 16,357 μg/mL; 10,428 μg/mL. Uji efek sedatif dilakukan dengan metode traction test  dan diperoleh hasil ekstrak etanol dan ketiga fraksi memiliki pengaruh efek sedatif pada dosis 300 mg/kgBB dan 500 mg/kgBB dan dosis yang paling baik dalam memberikan pengaruh efek sedatif adalah 500 mg/kgBB. ABSTRACT Nutmeg is a plant that is included in a type of medicinal plant. This study aimed to analyze the total phytochemical content and test the sedative effect of ethanol extract, petroleum ether fraction, ethyl acetate and nutmeg pulp. Extraction was carried out by maceration, then the ethanol extract obtained was fractionated by partitioning. Phytochemical analysis was carried out using UV-Vis spectrophotometry method so that the total phenolic content of ethanol extract, petroleum ether fraction, ethyl acetate and nutmeg water was obtained, respectively, 26.284 μg/mL; 29,203 μg/mL; 28.725 μg/mL; 24,904 μg/mL. The total flavonoids were 9.999 μg/mL, respectively; 13.095 μg/mL; 13,729 μg/mL; 10,475 μg/mL. The total tannins were 16,642 μg/mL, respectively; 16,571 μg/mL; 16,357 μg/mL; 10,428 μg/mL. The sedative effect test was carried out by the traction test method and the results of the ethanol extract and the three fractions had a sedative effect at a dose of 300 mg/kgBB and 500 mg/kgBB and the best dose in giving a sedative effect was 500 mg/kgBB.

scholarly journals Antibacterial Activity of the Red Algae Eucheuma cottonii Extract from Tanjung Coast, Sumenep Madura

ALCHEMY ◽  
2016 ◽  
Vol 4 (2) ◽  
pp. 93 ◽  
Author(s):  
Zulli Andriani ◽  
Ahmad Ghanaim Fasya ◽  
Ahmad Hanapi
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Red Algae ◽  
Methanol Extract ◽  
Petroleum Ether Extract ◽  
Inhibition Zone ◽  
Chloroform Extract ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Eucheuma Cottonii

<p align="center"><strong>Abstract</strong></p><p>Red alge <em>Euchema cottonii</em> is one of the biological source that has benefits. The aim of this study is to determine the effectiveness of antibacterial red algae <em>Eucheuma cottonii</em> extract<em>. </em>Isolation of active compounds was performed by maceration method using methanol. The methanol extract was hydrolyzed using HCl 2 N and partitioned by ethyl acetate, chloroform, petroleum ether and <em>n</em>-hexane solvents. The antibacterial assessment was determined using disc diffusion method againts bacteria <em>Staphylococcus aureus </em>and <em>Escherichia coli</em>. Identification of active compound was conducted by reagents test. The results showed that the best solvent to extract algae was methanol with 20.7% rendemen. The methanol extract 4% gives the highest inhibition at concentrations was 7.85 mm; followed by <em>n</em>-hexane and chloroform were 1.0 and 0.6 mm. However, bath ethyl acetate and petroleum ether extract depicted no inhibition zone. The identification test showed the presence of flavonoids, triterpenoids, steroids and alkaloids in the methanol extract. The extract of n-hexane contained triterpenoids and alkaloids, while the chloroform extract contained flavonoids, triterpenoids and alkaloids.</p><p> </p><p><strong>Keyword</strong><strong>s</strong>:  <em>Euchema cottonii</em>, antibacterial, disc diffusion<em></em></p>

Characteristics of Anti-Fungal Activity to Alternaria alternate of Different Proportion of Extraction from Sunflower Discs and Stalks

2019 ◽  
Vol 11 (11) ◽  
pp. 1561-1565
Author(s):  
Jialei Li ◽  
Ping Zhao ◽  
Ying Zhao ◽  
Li Tian ◽  
Zhenji Tian ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Ethyl Acetate Fraction ◽  
Petroleum Ether Fraction ◽  
Fungal Activity ◽  
Ether Fraction ◽  
Anti Fungal ◽  
Alternaria Alternate

The characteristics of anti-fungal activity to Alternaria alternate of three fractions of water, ethyl acetate, petroleum ether of sunflower discs and stalks have been studied. The fractions of water, ethyl acetate, petroleum ether of sunflower disc and petroleum ether fraction of sunflower stalk have good anti-fungal activity to A. alternate. The best concentrations of anti-fungal activity to A. alternate of water, ethyl acetate, petroleum ether fractions and petroleum ether fraction of sunflower stalk were 320 mg/mL, 240 mg/mL, 160 mg/mL and 160 mg/mL, respectively. The anti-fungal activities to A. alternate of these four kinds of extract are increased with the increase in concentration and arrive at stability after the best concentration of anti-fungal activity to A. alternate. The ethyl acetate fraction and petroleum ether fraction of sunflower stalk had no anti-fungal activity to A. alternate.

scholarly journals PHYTOCHEMICAL INVESTIGATION OF LEAVES AND SEEDS OF CORCHORUS OLITORIUS L. CULTIVATED IN IRAQ

2018 ◽  
Vol 11 (11) ◽  
pp. 408 ◽  
Author(s):  
Hayder T Hasan ◽  
Enas J Kadhim
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Phenolic Acid ◽  
High Performance ◽  
Corchorus Olitorius ◽  
Octadecanoic Acid ◽  
Plant Materials ◽  
Petroleum Ether Fraction ◽  
Phytochemical Investigation ◽  
Ether Fraction

Objective: The aim of this study was to investigate chemical constituents of leaves and seeds of Corchorus olitorius since no phytochemical investigation had been done previously in Iraq.Methods: Leaves and seeds of C. olitorius were defatted by maceration in hexane for 24 h. The defatted plant materials were extracted using Soxhlet apparatus, the aqueous methanol 85% as a solvent extraction for 24 h, and fractionated by petroleum ether, chloroform, ethyl acetate, and n-butanol. The ethyl acetate, n-butanol, and n-butanol after hydrolysis fractions for each part (leaves and seeds) were analyzed by high-performance liquid chromatography (HPLC) and high-performance thin-layer chromatography (HPTLC) for its phenolic acid and flavonoid contents. The petroleum ether fraction from the leaves was analyzed using Gas chromatography/mass spectrometer (GC/MS). Flavonoids and phenolic acid derivative were isolated from the ethyl acetate of leaf fraction and n-butanol after hydrolysis fraction of the seeds and identified by mass spectrometry, infrared, HPLC, and HPTLC.Results: The different chromatographic and spectroscopic results revealed the presence of luteolin, quercetin, astragalin, isoquercetin, catechins, and 3,5-dicaffeoylquinic acid (3,5-DCQA) in both leaves and seeds of C. olitorius and also 17-octadecynoic acid, 9-octadecanoic acid (oleic acid), hexadecenoic acid (palmitic acid), 9,12-octadecanoic acid (linoleic acid), octadecadien-1-ol (β-sitosterol), and α-tocopherol (Vitamin E) in the petroleum ether fraction of C. olitorius leaves.Conclusion: The results of the current study proved the presence of 3,5-DCQA, astragalin, and isoquercetin in the ethyl acetate fraction of C. olitorius leaves and catechin in the n-butanol after hydrolysis fraction of C. olitorius seeds. 

scholarly journals Isolation, identification and bioactivity of steroids compounds from red algae Eucheuma cottonii petroleum ether fraction

2019 ◽  
Author(s):  
Ahmad Ghanaim Fasya ◽  
Ahmad Baderos ◽  
Armeida Dwi Ridhowati Madjid ◽  
Suci Amalia ◽  
Dewi Sinta Megawati
Keyword(s):  
Petroleum Ether ◽  
Red Algae ◽  
Petroleum Ether Fraction ◽  
Ether Fraction ◽  
Eucheuma Cottonii

scholarly journals AKTIVITAS PENANGKAL RADIKAL BEBAS DARI FRAKSI KULIT KAYU SAGU BARUK (Arenga microcarpha Beccari)

PHARMACON ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 762
Author(s):  
Gerild Adrian ◽  
Edi Suryanto ◽  
Defny S. Wewengkang
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Ethyl Acetate Fraction ◽  
Petroleum Ether Fraction ◽  
Ether Fraction ◽  
Better Than

ABSTRACTThis study aims to determine the antioxidant activity and free radical antidote from fraction of bark sago baruk (Arenga microcarpha Beccari). This study initiated by extracting the powder of bark sago baruk using the maceration method for 3 days with ethanol 80%. The extract then partitioned using a series of solvent such as petroleum ether, ethyl acetate, buthanol, aquadest. The results showed that the ethyl acetate fraction had the highest free radical antidote content followed by the aquadest fraction, buthanol fraction, petroleum ether fraction. The content of free radical antidote respectively was 86,25%; 66,30%; 65,32%; 43,43%. Based on this study, the ethyl acetate fraction was the best fraction can act as an antidote to free radicals better than other fractions. Keywords: Bark sago baruk, fraction, free radical antidote ABSTRAKPenelitian ini bertujuan untuk menentukan Aktivitas penangkal radikal bebas dari fraksi kulit kayu sagu baruk (Arenga microcarpha beccari). Penelitian ini dimulai dengan mengekstraksi serbuk kulit kayu sagu baruk menggunakan cara maserasi selama 3 hari dengan pelarut etanol 80%. Ekstrak kemudian dipartisi menggunakan pelarut petroleum eter, etil asetat, butanol, dan aquades. kemudian ditentukan aktivitas antioksidan dan penangkal radikal bebas. Hasilnya menunujukan bahwa fraksi etil asetat memiliki kandungan penangkal radikal bebas tertinggi diikuti fraksi aquades, fraksi butanol, dan fraksi petroleum eter. Kandungan penangkal radikal bebas berturut-turut adalah 86,25%; 66,30%; 65,32%; 43,43%. Berdasarkan penelitian ini, dapat disimpulkan bahwa fraksi etil asetat dapat berperan sebagai penangkal radikal bebas lebih baik dibandingkan dengan fraksi lainnya. Kata Kunci:  Kulit Kayu Sagu Baruk, Fraksi, Penangkal Radikal Bebas

Isolation of the antidiarrhoeal tiliroside and its derivative from Waltheria indica leaf extract

2021 ◽  
Vol 25 (1) ◽  
pp. 86-92
Author(s):  
B.A. Ayinde ◽  
J.O. Owolabi ◽  
I.S. Uti ◽  
P.C. Ogbeta ◽  
M.I. Choudhary
Keyword(s):  
Silica Gel ◽  
Column Chromatography ◽  
Methanol Extract ◽  
Preparative Thin Layer Chromatography ◽  
Inhibitory Effects ◽  
Aqueous Fraction ◽  
Vacuum Liquid Chromatography ◽  
Ileum Contraction ◽  
Layer Chromatography ◽  
Dose Dependent

The antidiarrhoeal effect of Waltheria indica methanol extract and fractions have been reported earlier but, the present work examined the intestinal relaxant effects of two flavonoid-phenyl propanoids isolated from the methanol extract. The active aqueous fraction was subjected to vacuum liquid chromatography using dichloromethane with increasing concentration of ethyl acetate, and that of methanol and water successively. The ten (10) fractions obtained were combined to give seven (7). The fraction 2 (C, D) was subjected to preparative thin layer chromatography on silica gel GF254 (10-40μm) using CHCl3-CH3OH (8:2) to obtain compound coded F2. Fraction 4 (F) was subjected to column chromatography using silica gel (60-120μm mesh) and eluted with  dichloromethane with increasing concentrations of methanol. Fractions 9-28 were combined and subjected to column  chromatography using chloroform with increasing concentration of methanol. The fractions 1-16 of these were purified on Sephadex LH-20 to obtain compound BAA. The identities of the two compounds were established using spectroscopic methods. The  antidiarrheal effect of compound F2 was evaluated on mice using charcoal transit (100,200, 400mg/kg), castor oil (40, 60 mg/kg)  while the two compounds were examined for their inhibitory effects on Ach-induced ileum contraction. The effects of the  compounds were compared with loperamide (3mg/kg) and atropine (80μg). Compounds F2 and BAA were identified as tiliroside and 3’’’, 5’’’-dimethoxy tiliroside respectively. Tiliroside inhibited the charcoal transition in the animals in a dose dependent pattern with 400mg/ mL eliciting 63.41% inhibition compared to 59.23% produced by loperamide. The compound also elicited significantly (P<0.05) prolonged onset of stooling and reduced the number and weight of stools produced lower than the control. The two  compounds drastically inhibited the Ach-induced contractions of the ileum. The compound, tiliroside at 10mg, completely abolished  the contraction by Ach unlike 3’’’, 5’’’-dimethoxy tiliroside which reduced the contraction to 1.92% at 20mg. The identified compounds seem to be responsible for the ethnomedicinal use of the plant in treating diarrhea.

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