To produce a quality bridge then it should be taken into account with the correct parameters and the condition of the bridge. One of the parameters is an important bridge natural frequency incurred due to dynamic loads. The purpose of this study was to conduct a comparative analysis of the natural frequency of bridge girder value measurement results in the field of natural frequency calculation results using Midas Civil programs and manuals, as well as knowing the value of the condition value of natural frequency of bridge between the actual value of natural frequency measurement results on the field and the value of natural frequency calculation program results Midas Civil. In the analysis, use design bridge gelagar PCI with a span of 21,95 meters. And bridge specifications will be made from the analysis of the natural frequency of the load calculation of structures with Midas program manual and Civil that would then be compared with the results of field measurements to find out the condition of the bridge. The natural frequency obtained from calculation manual for a bridge girder single of 5,95 Hz, and multi girder of 5,72 Hz.While value natural frequency of program midas civil for singles girder of 6,16 Hz, and for multi girder of 5,93Hz.