Depth distributions of signaling molecules in Pseudomonas aeruginosa biofilms mapped by confocal Raman microscopy

2021 ◽  
Vol 154 (20) ◽  
pp. 204201
Author(s):  
Tianyuan Cao ◽  
Abigail A. Weaver ◽  
Seol Baek ◽  
Jin Jia ◽  
Joshua D. Shrout ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Signaling Molecules ◽  
Raman Microscopy ◽  
Confocal Raman Microscopy ◽  
Confocal Raman ◽  
Depth Distributions

scholarly journals Surface-Growing Communities of Pseudomonas aeruginosa Exhibit Distinct Alkyl Quinolone Signatures

2018 ◽  
Vol 11 ◽  
pp. 117863611881773 ◽  
Author(s):  
Nydia Morales-Soto ◽  
Tianyuan Cao ◽  
Nameera F Baig ◽  
Kristen M Kramer ◽  
Paul W Bohn ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Quorum Sensing ◽  
Raman Microscopy ◽  
Confocal Raman Microscopy ◽  
Interspecies Competition ◽  
Microbial Biofilm ◽  
Pseudomonas Quinolone Signal ◽  
Confocal Raman ◽  
Spatial Identification

A cascade of events leads to the development of microbial biofilm communities that are thought to be responsible for over 80% of infections in humans. However, not all surface-growing bacteria reside in a stationary biofilm state. Here, we have employed confocal Raman microscopy to analyze and compare variations in the alkyl quinolone (AQ) family of molecules during the transition between surface-attached motile-swarming and stationary biofilm communities. The AQs have been established previously as important to Pseudomonas aeruginosa biofilms, interspecies competition, and virulence. The AQ Pseudomonas quinolone signal (PQS) is also a known quorum-sensing signal. We detail spatial identification of AQ, PQS, and 2-alkyl-4-hydroxyquinoline N-oxide (AQNO) metabolites in both swarm and biofilm communities. We find that AQNO metabolites are abundant signatures in active swarming communities.

A Confocal Raman Microscopy Study of the Distribution of a Carotene-Containing Yeast in a Living Pseudomonas Aeruginosa Biofilm

2008 ◽  
Vol 62 (9) ◽  
pp. 975-983 ◽  
Author(s):  
Christophe Sandt ◽  
Truis Smith-Palmer ◽  
Judith Pink ◽  
David Pink
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Microscopy Study ◽  
Raman Microscopy ◽  
Confocal Raman Microscopy ◽  
Confocal Raman

Confocal Raman Microscopy, Synchrotron X-ray Diffraction, and Photoacoustic Study of Ba0.85Ca0.15Ti0.90Zr0.10O3: Understanding Structural and Microstructural Response to the Electric Field

2021 ◽  
Author(s):  
Armando Reyes-Montero ◽  
Fernando Rubio-Marcos ◽  
Luis Edmundo Fuentes-Cobas ◽  
Adolfo Del Campo ◽  
Rosalba Castañeda-Guzmán ◽  
...  
Keyword(s):  
Electric Field ◽  
Raman Microscopy ◽  
Confocal Raman Microscopy ◽  
X Ray Diffraction ◽  
X Ray ◽  
Confocal Raman

Depth profiling of strain and carrier concentration by cleaved surface scanning of GaN Gunn-diode: confocal Raman microscopy

2013 ◽  
Vol 28 (10) ◽  
pp. 105011 ◽  
Author(s):  
A E Belyaev ◽  
V V Strelchuk ◽  
A S Nikolenko ◽  
A S Romanyuk ◽  
Yu I Mazur ◽  
...  
Keyword(s):  
Carrier Concentration ◽  
Depth Profiling ◽  
Raman Microscopy ◽  
Gunn Diode ◽  
Confocal Raman Microscopy ◽  
Surface Scanning ◽  
Confocal Raman

Optically Trapping Confocal Raman Microscopy of Individual Lipid Vesicles:  Kinetics of Phospholipase A2-Catalyzed Hydrolysis of Phospholipids in the Membrane Bilayer

2006 ◽  
Vol 78 (19) ◽  
pp. 6928-6935 ◽  
Author(s):  
Daniel P. Cherney ◽  
Grant A. Myers ◽  
Robert A. Horton ◽  
Joel M. Harris
Keyword(s):  
Phospholipase A2 ◽  
Lipid Vesicles ◽  
Raman Microscopy ◽  
Confocal Raman Microscopy ◽  
Membrane Bilayer ◽  
Confocal Raman ◽  
Kinetics Of ◽  
Hydrolysis Of

Single Layer Graphene for Estimation of Axial Spatial Resolution in Confocal Raman Microscopy Depth Profiling

2018 ◽  
Vol 91 (1) ◽  
pp. 1049-1055 ◽  
Author(s):  
Carol Korzeniewski ◽  
Jay P. Kitt ◽  
Saheed Bukola ◽  
Stephen E. Creager ◽  
Shelley D. Minteer ◽  
...  
Keyword(s):  
Spatial Resolution ◽  
Depth Profiling ◽  
Single Layer ◽  
Raman Microscopy ◽  
Single Layer Graphene ◽  
Confocal Raman Microscopy ◽  
Layer Graphene ◽  
Confocal Raman

scholarly journals Real-Time Drug Release Imaging From Nanocomposite Drug and Polymer Coatings

2008 ◽  
Vol 2 (2) ◽  
Author(s):  
Jinping Dong ◽  
Greg Haugstad ◽  
Chris Frethem ◽  
John Foley ◽  
Bob Hoerr ◽  
...  
Keyword(s):  
Drug Release ◽  
Real Time ◽  
Polymer Coatings ◽  
Raman Microscopy ◽  
Prolonged Release ◽  
Confocal Raman Microscopy ◽  
Release Process ◽  
Force Microscopy ◽  
Confocal Raman ◽  
Model Drug

The ElectroNanospray process (Nanocopoeia, Inc) transforms drugs and polymers into many nanoscale material states including powders, liquids, encapsulated particles, and coatings. This allows application of polymers and drugs to the surface of medical devices such as coronary stents in a single-stage process. A model drug delivery system consisting of a polymer matrix (arborescent polyisobutylene-polystyrene, or arbIBS) and either dexamethasone or sirolimus was studied by various characterization techniques. Modification of ElectroNanospray process parameters resulted in surface coatings with rich morphologies that are revealed by SEM, Atomic Force Microscopy (AFM) and Confocal Raman Microscopy were employed to monitor the drug release process in situ, through which the mechanism of the drug-eluting process may be proposed. A Confocal Raman microscope fitted with underwater objective was used to image arbIBS∕drug films incubated in phosphate-buffered saline over 12h and at various film depths. Drug migrated from more concentrated areas into the surrounding polymer and toward the surface, beginning as early as 5min after placing the sample in buffer and continuing throughout the 12h period. High drug levels remained in the more concentrated areas at the end of incubation, suggesting the potential for prolonged release. SEM and AFM images taken from samples post incubation showed the appearance of nanoscale pores ∼100nm in diameter in areas corresponding in size and distribution to the Confocal Raman planar image areas of increased drug concentration. Confocal Raman microscopy offers a powerful new technique for demonstrating real-time drug release from therapeutic medical device coatings.

scholarly journals Label-Free Live-Cell Imaging with Confocal Raman Microscopy

2012 ◽  
Vol 102 (2) ◽  
pp. 360-368 ◽  
Author(s):  
Katharina Klein ◽  
Alexander M. Gigler ◽  
Thomas Aschenbrenner ◽  
Roberto Monetti ◽  
Wolfram Bunk ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Raman Microscopy ◽  
Live Cell ◽  
Confocal Raman Microscopy ◽  
Label Free ◽  
Confocal Raman
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