Long-lived Ag106+ luminescence and a split DNA scaffold

DNA origami is typically used to fold a long single-stranded DNA scaffold into nanostructures with complex geometries using many short DNA staple strands. Integration of RNA into nucleic acid nanostructures...

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DNA binding adaptors to assemble proteins of interest on DNA scaffold

Methods in Enzymology - Metabolons and Supramolecular Enzyme Assemblies ◽

10.1016/bs.mie.2018.12.014 ◽

2019 ◽

pp. 287-322

Author(s):

Eiji Nakata ◽

Huyen Dinh ◽

Thang Minh Nguyen ◽

Takashi Morii

Keyword(s):

Dna Binding ◽

Dna Scaffold

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HaloTag mediated artificial cellulosome assembly on a rolling circle amplification DNA template for efficient cellulose hydrolysis

Chemical Communications ◽

10.1039/c6cc02035f ◽

2016 ◽

Vol 52 (40) ◽

pp. 6701-6704 ◽

Cited By ~ 14

Author(s):

Qing Sun ◽

Wilfred Chen

Keyword(s):

Rolling Circle Amplification ◽

Cellulose Hydrolysis ◽

The Self ◽

Rolling Circle ◽

Dna Template ◽

Dna Scaffold

We report here the generation of artificial cellulosomes onto a DNA scaffold using the self-labeling HaloTag for DNA conjugation. Rolling circle amplification multiplexing templates were used to increase the complexity of this system with higher efficiency observed.

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Evaluation of the role of the DNA surface for enhancing the activity of scaffolded enzymes

Chemical Communications ◽

10.1039/d1cc00276g ◽

2021 ◽

Vol 57 (32) ◽

pp. 3925-3928

Author(s):

Peng Lin ◽

Huyen Dinh ◽

Yuki Morita ◽

Zhengxiao Zhang ◽

Eiji Nakata ◽

...

Keyword(s):

Ph Changes ◽

Enhanced Activity ◽

Local Ph ◽

Dna Scaffold

Local pH changes on the DNA scaffold surface do not contribute to the enhanced activity of scaffolded enzymes.

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Interacting Behavior of Two Myosin Va Motors Coupled via a DNA Scaffold

Biophysical Journal ◽

10.1016/j.bpj.2011.11.3094 ◽

2012 ◽

Vol 102 (3) ◽

pp. 568a ◽

Cited By ~ 1

Author(s):

Hailong Lu ◽

Carol S. Bookwalter ◽

Elena B. Krementsova ◽

Michael R. Diehl ◽

Kathleen M. Trybus

Keyword(s):

Myosin Va ◽

Dna Scaffold

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Binding His-tagged Proteins to NTA Stripes Assembled in 2D DNA Scaffold

Chinese Journal of Chemistry ◽

10.1002/cjoc.201090301 ◽

2010 ◽

Vol 28 (10) ◽

pp. 1795-1798 ◽

Cited By ~ 5

Author(s):

Wenyi Huang ◽

Jie Chao ◽

Qin Yan ◽

Shoujun Xiao

Keyword(s):

Dna Scaffold

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Cobalt tungsten oxide hydroxide hydrate (CTOHH) on DNA scaffold: an excellent bi-functional catalyst for oxygen evolution reaction (OER) and aromatic alcohol oxidation

Dalton Transactions ◽

10.1039/c9dt03941d ◽

2019 ◽

Vol 48 (45) ◽

pp. 17117-17131 ◽

Cited By ~ 4

Author(s):

Sangeetha Kumaravel ◽

Prabaharan Thiruvengetam ◽

Sivasankara Rao Ede ◽

K. Karthick ◽

S. Anantharaj ◽

...

Keyword(s):

Tungsten Oxide ◽

Oxygen Evolution ◽

Oxygen Evolution Reaction ◽

Oxidation Reaction ◽

Alcohol Oxidation ◽

Aromatic Alcohol ◽

Oxide Hydroxide ◽

Hydroxide Hydrate ◽

Dna Scaffold

CTOHH-DNA, a newly developed catalyst utilized for both electrocatalytic OER and aromatic alcohol oxidation reaction with excellent activities.

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A modular approach for dCas9-mediated enzyme cascading via orthogonal bioconjugation

Chemical Communications ◽

10.1039/d0cc04196c ◽

2020 ◽

Vol 56 (77) ◽

pp. 11426-11428

Author(s):

Emily A. Berckman ◽

Wilfred Chen

Keyword(s):

Modular Approach ◽

One Pot ◽

Guided Assembly ◽

The One ◽

Enzyme Cascades ◽

Dna Scaffold

We report a new modular strategy to assemble dCas9-guided enzyme cascades by employing orthogonal post-translation chemistry to enable the one-pot bioconjugation and guided assembly of two different dCas9 enzymes onto a DNA scaffold.

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