scholarly journals A mini DNA-RNA hybrid origami nanobrick

2021 ◽  
Author(s):  
Lifeng Zhou ◽  
Arun Richard Chandrasekaran ◽  
Mengwen Yan ◽  
Vibhav A. Valsangkar ◽  
Jeremy I. Feldblyum ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Dna Origami ◽  
Complex Geometries ◽  
Single Stranded Dna ◽  
Dna Scaffold

DNA origami is typically used to fold a long single-stranded DNA scaffold into nanostructures with complex geometries using many short DNA staple strands. Integration of RNA into nucleic acid nanostructures...

scholarly journals Synthesis of DNA Origami Scaffolds: Current and Emerging Strategies

Molecules ◽  
2020 ◽  
Vol 25 (15) ◽  
pp. 3386 ◽  
Author(s):  
Joshua Bush ◽  
Shrishti Singh ◽  
Merlyn Vargas ◽  
Esra Oktay ◽  
Chih-Hsiang Hu ◽  
...  
Keyword(s):  
Biomedical Applications ◽  
Large Scale ◽  
Structural Complexity ◽  
Dna Origami ◽  
Single Stranded Dna ◽  
Promising Tool ◽  
Design Flexibility ◽  
Scalable Synthesis ◽  
Scalable Methods ◽  
Dna Scaffold

DNA origami nanocarriers have emerged as a promising tool for many biomedical applications, such as biosensing, targeted drug delivery, and cancer immunotherapy. These highly programmable nanoarchitectures are assembled into any shape or size with nanoscale precision by folding a single-stranded DNA scaffold with short complementary oligonucleotides. The standard scaffold strand used to fold DNA origami nanocarriers is usually the M13mp18 bacteriophage’s circular single-stranded DNA genome with limited design flexibility in terms of the sequence and size of the final objects. However, with the recent progress in automated DNA origami design—allowing for increasing structural complexity—and the growing number of applications, the need for scalable methods to produce custom scaffolds has become crucial to overcome the limitations of traditional methods for scaffold production. Improved scaffold synthesis strategies will help to broaden the use of DNA origami for more biomedical applications. To this end, several techniques have been developed in recent years for the scalable synthesis of single stranded DNA scaffolds with custom lengths and sequences. This review focuses on these methods and the progress that has been made to address the challenges confronting custom scaffold production for large-scale DNA origami assembly.

scholarly journals Novel nucleic acid origami structures and conventional molecular beacon–based platforms: a comparison in biosensing applications

2021 ◽  
Author(s):  
Noemi Bellassai ◽  
Roberta D’Agata ◽  
Giuseppe Spoto
Keyword(s):  
Nucleic Acid ◽  
Structural Properties ◽  
Molecular Beacon ◽  
Dna Origami ◽  
Molecular Beacons ◽  
Conformational Polymorphism ◽  
Functional Systems ◽  
Similarities And Differences ◽  
Nucleic Acid Structures ◽  
Nucleic Acid Sequences

AbstractNucleic acid nanotechnology designs and develops synthetic nucleic acid strands to fabricate nanosized functional systems. Structural properties and the conformational polymorphism of nucleic acid sequences are inherent characteristics that make nucleic acid nanostructures attractive systems in biosensing. This review critically discusses recent advances in biosensing derived from molecular beacon and DNA origami structures. Molecular beacons belong to a conventional class of nucleic acid structures used in biosensing, whereas DNA origami nanostructures are fabricated by fully exploiting possibilities offered by nucleic acid nanotechnology. We present nucleic acid scaffolds divided into conventional hairpin molecular beacons and DNA origami, and discuss some relevant examples by focusing on peculiar aspects exploited in biosensing applications. We also critically evaluate analytical uses of the synthetic nucleic acid structures in biosensing to point out similarities and differences between traditional hairpin nucleic acid sequences and DNA origami. Graphical abstract

scholarly journals Single-Stranded DNA Curtains for Real-Time Single-Molecule Visualization of Protein–Nucleic Acid Interactions

2012 ◽  
Vol 84 (18) ◽  
pp. 7607-7612 ◽  
Author(s):  
Bryan Gibb ◽  
Tim D. Silverstein ◽  
Ilya J. Finkelstein ◽  
Eric C. Greene
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Single Molecule ◽  
Single Stranded Dna ◽  
Protein Nucleic Acid ◽  
Nucleic Acid Interactions

scholarly journals Influence of nucleic acid base aromaticity on substrate reactivity with enzymes acting on single-stranded DNA

1993 ◽  
Vol 21 (3) ◽  
pp. 621-626 ◽  
Author(s):  
Michael Weinfeld ◽  
Krista-June M. Soderlind ◽  
Garry W. Buchko
Keyword(s):  
Nucleic Acid ◽  
Acid Base ◽  
Single Stranded Dna ◽  
Nucleic Acid Base ◽  
Substrate Reactivity

Highly stable triple helix formation by homopyrimidine (l)-acyclic threoninol nucleic acids with single stranded DNA and RNA

2015 ◽  
Vol 13 (8) ◽  
pp. 2366-2374 ◽  
Author(s):  
Vipin Kumar ◽  
Venkitasamy Kesavan ◽  
Kurt V. Gothelf
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Triple Helix ◽  
Helical Structures ◽  
Single Stranded Dna ◽  
Dna And Rna ◽  
Helix Formation ◽  
Triple Helix Formation

Homopyrimidine acyclic (l)-threoninol nucleic acid (aTNA) was synthesized and found to form highly stable (l)-aTNA–DNA–(l)-aTNA and (l)-aTNA–RNA–(l)-aTNA triple helical structures.

Conformationally Locked Aryl C-Nucleosides: Synthesis of Phosphoramidite Monomers and Incorporation into Single-Stranded DNA and LNA (Locked Nucleic Acid).

ChemInform ◽  
2003 ◽  
Vol 34 (15) ◽  
Author(s):  
B. Ravindra Babu ◽  
Ashok K. Prasad ◽  
Smriti Trikha ◽  
Niels Thorup ◽  
Virinder S. Parmar ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Locked Nucleic Acid ◽  
Single Stranded Dna ◽  
Conformationally Locked

scholarly journals Nuclease-Resistant Single-Stranded DNA Controls for Nucleic Acid Amplification Assays

2007 ◽  
Vol 45 (8) ◽  
pp. 2570-2574 ◽  
Author(s):  
A. Gotsch ◽  
A. Schubert ◽  
A. Bombis ◽  
M. Wiedmann ◽  
M. Zauke ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Amplification ◽  
Single Stranded Dna
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