scholarly journals Sunflower meal and spring pea ruminal degradation protection using malic acid or orthophosphoric acid-heat treatments

2016 ◽  
Vol 56 (12) ◽  
pp. 2029 ◽  
Author(s):  
F. Díaz-Royón ◽  
J. M. Arroyo ◽  
M. D. Sánchez-Yélamo ◽  
J. González
Keyword(s):  
Organic Matter ◽  
Crude Protein ◽  
Dry Matter ◽  
Microbial Contamination ◽  
Orthophosphoric Acid ◽  
Sunflower Meal ◽  
Ruminal Degradation ◽  
Electrophoretic Techniques ◽  
Intestinal Digestibility

The effects of solutions of malic or orthophosphoric acids (0.752 Eqg/kg of feed) and heat to protect proteins of sunflower meal (SFM) and spring pea (SP) against ruminal degradation were studied using particle transit, 15N infusion, in situ and electrophoretic techniques. Three wethers fitted with rumen and duodenum cannulae were successively fed three isoproteic diets including SFM and SP, untreated or treated with malic or orthophosphoric acids. Incubations of tested meals were only performed while feeding the respective diet. Estimates of the ruminally undegraded fraction (RU) and its intestinal digestibility of dry matter, organic matter (only for RU), crude protein and starch (only in SP) were obtained considering ruminal microbial contamination and particle comminution and outflow rates. When corrected for microbial contamination, estimates of RU and intestinal digestibility decreased in all tested fractions for both feeds. All RU estimates increased with the protective treatments, whereas intestinal digestibility-dry matter also increased in SFM. Low intestinal digestibility-crude protein values suggested the presence of antitrypsin factors in SP. Protective treatments of both feeds led to consistent increases in the intestinal digested fraction of dry matter and crude protein, being only numerically different for SP-starch (60.5% as average). However, treatments also reduced the organic matter fermentation, which may decrease ruminal microbial protein synthesis. Electrophoretic studies showed albumin disappearance in both SFM and SP, whereas changes in other RU proteins were more pronounced in SP than SFM.

In situ degradability of organic matter, crude protein and cell wall of various tree forages

1992 ◽  
Vol 55 (1) ◽  
pp. 29-34 ◽  
Author(s):  
P. Kamatali ◽  
E. Teller ◽  
M. Vanbelle ◽  
G. Collignon ◽  
M. Foulon
Keyword(s):  
Cell Wall ◽  
Organic Matter ◽  
Leucaena Leucocephala ◽  
Crude Protein ◽  
In Vitro Digestibility ◽  
Sesbania Sesban ◽  
Holstein Friesian ◽  
Ruminal Degradation

AbstractLeaves of Leucaena leucocephala, Sesbania sesban and Calliandra callothyrsus were harvested in Rwanda from young shoots at 8 weeks after the first cutting. They were dried, ground and incubated at the same time in polyester bags in three non-lactating Holstein-Friesian cows each fitted with a ruminal cannula. The bags were removed at 0, 2, 4, 8, 24, 48, 72 and 144 h after the start of incubations. The different parameters characterizing extent and rate of ruminal degradation of organic matter (OM), neutral-detergent fibre (NDF), and crude protein (CP) were calculated. In vitro digestibility of residual protein after 24 h and 48 h incubation was also determined. Sesbania sesban had lowest cell wall contents and gave highest ruminal degradability for OM, NDF and CP. Leucaena leucocephala was degraded to a lesser extent, but its undegraded protein had a somewhat higher in vitro digestibility. In contrast, protein of Calliandra callothyrsus was poorly degraded and digested. The proportion and composition of cell wall could not explain these differences in digestion characteristics and other measurements, such as tannins, were incriminated. Increased ruminal incubation time augmented the extent of ruminal degradation and reduced in vitro digestibility of undegraded protein but did not affect the undigestible protein fraction.

Effect of diet forage to concentrate ratio on rumen degradability and post-ruminal availability of protein from fresh and dried lucerne

2002 ◽  
Vol 74 (2) ◽  
pp. 337-345 ◽  
Author(s):  
J. Faría-Mármol ◽  
J. González ◽  
C.A. Rodríguez ◽  
M. R. Alvir
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Degradation Rate ◽  
Close Correlation ◽  
Insoluble Fraction ◽  
Exponential Equation ◽  
Mean Values ◽  
Two Samples ◽  
Ruminal Degradation ◽  
Intestinal Digestibility

AbstractThe ruminal degradation of dry matter (DM) and crude protein (CP) and the intestinal availability of CP of four lucerne samples were measured on two diets with lucerne hay to concentrate ratios of 2: 1 (diet F) and 1: 2 (diet C). Two samples of fresh lucerne (third cut) harvested after 2 (FL1) or 8 (FL2) weeks from the previous cut were used together with a sample of lucerne hay (LH) and another of dehydrated lucerne (DL). Rumen degradability, measured by the nylon bag technique, and rumen outflow rates were determined on three rumen cannulated wethers. Intestinal digestibility was determined by the mobile bag technique on three duodenal fistulated wethers. For CP, significantly lower values were observed with diet C than with diet F for the potentially degradable insoluble fraction (0·334 v. 0·397) and its degradation rate (0·093 v. 0·134 per h). As a consequence, the effective degradability was also lower with diet C (0·746 v. 0·821; P = 0·059). Effective degradability of DM was also apparently lower with diet C (0·596 v. 0·634). With both diets, the intestinal digestibility decreased in all the samples with increase of ruminal incubation time according to a simple exponential equation. The undegraded CP digested in the gut (Di) and therefore the effective intestinal digestibility (EID) were derived from this exponential function according to the rumen outflow of undegraded CP. Mean values of Di (expressed as proportion of food CP content) were respectively 0·091 and 0·142 for F and C diets and 0·084, 0·115, 0·116, and 0·152 for FL1, FL2, LH and DL samples. Lower rumen degradability was partially compensated for by higher Di values resulting in a close correlation between both parameters (r = –0·965; P 0·001). The change of the digestion site associated with the reduction of the effective degradability of CP produced also an increase in the undigested CP as a proportion of food CP. So, these values are respectively 0·087 and 0·112 for F and C diets and 0·053, 0·109, 0·096, and 0·141 for FL1, FL2, LH, and DL samples. No difference in EID between F and C diets was observed (0·529 v. 0·563). For samples, the only effect (P 0·05) was recorded between FL1 (0·618) and the other samples (0·509, 0·544 and 0·512 for FL2, LH, and DL, respectively).

Determination of degradation in the rumen for dry matter, organic matter and crude protein of alfalfa (Medicago sativa L.) harvested at differentmaturity stages using the in situ nylon bag method

2016 ◽  
Author(s):  
Elif Karayilanli ◽  
Veysel Ayhan
Keyword(s):  
Organic Matter ◽  
Medicago Sativa ◽  
Crude Protein ◽  
Dry Matter ◽  
Seed Setting ◽  
Maturity Stages ◽  
Mature Period ◽  
Medicago Sativa L

The aim of this study was to determine the degradation in the rumen for the dry matter (DM), organic matter (OM) and crude protein (CP) of alfalfa (Medicago sativa L.) harvested at different maturity stages by using an in situ nylon bag method. Maturity and harvesting effects were observed in ruminally degradable DM, OM and CP. High correlations between in situ degradability and maturity were determined. Alfalfa harvested in the bud period exhibited more extensive ruminal DM, OM and CP degradation than that harvested at other maturity stages. The alfalfa hay’s DM, OM and CP degradability were lowest (62.18%, 59.52% and 76.31%, respectively) in its most mature period of seed setting; its degradability decreased with maturity (17%, 14% and 13%, respectively).

scholarly journals Effects of the correction of particle microbial contamination and particle transit model in the rumen on in situ protein evaluation of grass hays

2013 ◽  
Vol 53 (2) ◽  
pp. 134 ◽  
Author(s):  
J. A. Guevara-González ◽  
J. González ◽  
J. M. Arroyo ◽  
V. J. Moya ◽  
O. Piquer
Keyword(s):  
Amino Acids ◽  
Crude Protein ◽  
Microbial Contamination ◽  
Ruminal Fermentation ◽  
Degradation Pattern ◽  
Ryegrass Hay ◽  
Variable Errors ◽  
Composite Samples ◽  
Intestinal Digestibility

Effects of considering the particle comminution rate (kc) in addition to particle rumen outflow (kp) and the ruminal microbial contamination on estimates of by-pass and intestinal digestibility of DM, organic matter and crude protein were examined in perennial ryegrass and oat hays. By-pass kc-kp-based values of amino acids were also determined. This study was performed using particle transit, in situ and 15N techniques on three rumen and duodenum-cannulated wethers. The above estimates were determined using composite samples from rumen-incubated residues representative of feed by-pass. Considering the comminution rate, kc, modified the contribution of the incubated residues to these samples in both hays and revealed a higher microbial contamination, consistently in oat hay and only as a tendency for crude protein in ryegrass hay. Not considering kc or rumen microbial contamination overvalued by-pass and intestinal digestibility in both hays. Therefore, non-microbial-corrected kp-based values of intestinal digested crude protein were overestimated as compared with corrected and kc-kp-based values in ryegrass hay (17.4 vs 4.40%) and in oat hay (5.73 vs 0.19%). Both factors should be considered to obtain accurate in situ estimates in grasses, as the protein value of grasses is very conditioned by the microbial synthesis derived from their ruminal fermentation. Consistent overvaluations of amino acid by-pass due to not correcting microbial contamination were detected in both hays, with large variable errors among amino acids. A similar degradation pattern of amino acids was recorded in both hays. Cysteine, methionine, leucine and valine were the most degradation-resistant amino acids.

scholarly journals In situ ruminal degradation of sallow tree leaves using different mathematical models

2021 ◽  
Vol 26 (3) ◽  
pp. e2170
Author(s):  
Valiollah Palangi
Keyword(s):  
Mathematical Models ◽  
Crude Protein ◽  
Dry Matter ◽  
Gompertz Model ◽  
Exponential Model ◽  
Salix Alba ◽  
Estimated Parameters ◽  
Ruminal Degradation ◽  
Ruminal Degradability

Objective. The objective of this study was to determine the in situ digestion parameters of sallow leaves (Salix Alba) as an alternative food, not commonly used in animal diets using mathematical models. Materials and methods. Four mathematical models were used to describe the ruminal disappearance of dry matter (DM) and crude protein (CP) of sallow leaves: non lagged exponential (Model I); lagged exponential (Model II); Gompertz (Model III), and generalized Mitscherlich (Model IV). Results. Results of DM and CP degradability characteristics showed that all the models fitted well (r2>0.98) to the disappearance data. There were minor differences between the models in terms of statistical evaluations. However, the models differed in the estimated parameters depending on the model’s nature and structure and the parameters included. Conclusions. Given that some models estimated negative values for the studied parameters, they were not biologically acceptable. For this reason, the only model I can be used for estimating the degradability of DM and CP of sallow leaves. In terms of effective degradability (ED) of DM, model III was not used in the calculations because of calculating negative values for part A. Sallow leaves constitute the largest possible proportion of the ruminal degradability fractions, and effective degradability can be used as a forage source in ruminant feeds.

scholarly journals Degradabilidad in situ de la materia seca, proteína y fibra del forraje de morera (Morus alba).

2006 ◽  
Vol 12 (1) ◽  
pp. 79
Author(s):  
Carlos Boschini
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Soluble Fraction ◽  
Neutral Detergent Fiber ◽  
Whole Plant ◽  
Degradation Rates ◽  
Entire Plant ◽  
Ruminal Degradation ◽  
Ruminal Degradability

The purpose of this study was to determine the ruminal degradability of dry matter, crude protein, neutral detergent fiber and their fractions, contained in regrowth of mulberry at 56, 84 and 112 days. The degradation of leaves, stems and the whole plant was calculated. The potential degradability of these three components diminished as the regrowth aged. The soluble fraction of the dry matter was reduced from 29% to 25% in the leaves and from 27% to 19% in the stems between the 56th and the112th day. The degradable fraction in the stem remained constant (61-63%) on the three measuring days, with ruminal degradation levels of 14%, 10% and 9%/h respectively. In the stem the degradable fraction diminished from 42% to 30% between the 56th and 112th day, with degradation speeds of 8.4%, 6.7 % and 5.3%/h. The soluble crude protein diminished with age from 41% to 33% in the entire plant and the degradable fraction remained constant between 49% and 51%. The degradation rate was higher than 9.4%/h. The neutral detergent fiber had a degradable fraction of 71%, 62% y 43% on the 56th, 84th and 112th day, with degradation rates of 13%, 10% y 9%/h respectively. The degradation of the neutral detergent fiber began in the rumen with a lag time of 1.5 to 3.2 hours.

scholarly journals An investigation on determining the nutritive value of oak nuts

2011 ◽  
Vol 49 (No. 5) ◽  
pp. 211-219 ◽  
Author(s):  
B. Z Saricicek ◽  
U. Kilic
Keyword(s):  
Organic Matter ◽  
Crude Protein ◽  
Dry Matter ◽  
Nutritive Value ◽  
The Other ◽  
Feed System ◽  
Natural Production ◽  
Nutritive Values

This study was conducted to determine the nutritive values of shelled, unshelled oak nuts and their shells. For the estimation of dry matter (DM), organic matter (OM) and crude protein (CP), degradability characteristics, digestibilities, energy values and tannin contents of these feedstuffs the nylon bag technique, cellulose feed system and enzyme methods were used. In spite of the fact that in situ dry matter, organic matter and crude protein degradabilities of shelled and unshelled oak nuts were high, the degradabilities of shells were found lower. Degradability characteristics (a, b, a + b) and effective degradabilities were high in unshelled and shelled oak nuts and low in shells. The differences between the oak nut shells and the other two groups were significant for DM and OM degradabilities (P < 0.01). Pepsin digestible N contents were lower in shelled and unshelled oak nuts and high in shells. Unshelled and shelled oak nuts were significantly different from the oak nut shells for digestibility of DM and OM. Energy values were highest in unshelled oak nuts and lowest in shells. Oak nuts can be used as a forage source in regions with their high natural production.  

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