Fingerprinting of cauliflower cultivars using RAPD markers

2004 ◽  
Vol 55 (2) ◽  
pp. 117 ◽  
Author(s):  
Ida A. Astarini ◽  
Julie A. Plummer ◽  
Rachel A. Lancaster ◽  
Guijun Yan
Keyword(s):  
Phylogenetic Analysis ◽  
Brassica Oleracea ◽  
Rapd Markers ◽  
Genetic Relationships ◽  
Randomly Amplified Polymorphic Dna ◽  
Routine Identification ◽  
Polymorphic Bands

Randomly amplified polymorphic DNA (RAPD) was used to investigate genetic relationships among 25 cultivars of cauliflower (Brassica oleracea var. botrytis L.). Forty decamer primers were examined, among which 15 primers produced polymorphism. Twenty-five polymorphic bands were observed, ranging in size from 428 to 1646 bp. A fingerprinting key was generated using these polymorphic bands. A dendogram was constructed using neighbour-joining analysis based on phylogenetic analysis using parsimony (PAUP). Results indicate that RAPD markers can be used for the routine identification of cauliflower cultivars within B. oleracea var. botrytis L.

scholarly journals DNA Fingerprinting to Identify Nine Anthurium Pot Plant Cultivars and Examine Their Genetic Relationship

HortScience ◽  
2001 ◽  
Vol 36 (4) ◽  
pp. 758-760 ◽  
Author(s):  
D.G. Ranamukhaarachchi ◽  
R.J. Henny ◽  
C.L. Guy ◽  
Q.B. Li
Keyword(s):  
Genetic Relationship ◽  
Rapd Markers ◽  
Parental Species ◽  
Genetic Relationships ◽  
Dna Fingerprints ◽  
Randomly Amplified Polymorphic Dna ◽  
Arbitrary Primers ◽  
D Values ◽  
High Degree ◽  
Pot Plant

Randomly amplified polymorphic DNA (RAPD) markers were utilized to determine the genetic relationships of nine morphologically similar pot plant cultivars of Anthurium sp. by developing DNA fingerprints (DFP). Of 25 arbitrary primers screened, nine generated DFPs that were used in computing the genetic distance (d) and similarity coefficient (C) values. All cultivars tested exhibited a high degree of genetic similarity. `Lady Ann' and `Lady Beth' possessed the closest relationship with d and C values of 0.06 and 0.98, respectively. The next closest genetic relationship was between `Red Hot' and `Southern Blush' (d = 0.33, C = 0.89). These two cultivars exhibited a more distant relationship to the other seven cultivars as indicated by higher `d' values. However, this study showed that the nine Anthurium cultivars examined were genetically closely related. These cultivars share specific DNA bands with three possible parental species (A. andraeanum Linden ex Andre, A. antioquens L., and A. amnicola Dressler) included in this study, which may indicate similarities in their pedigree. This study shows that RAPDs can be a useful tool to distinguish Anthurium pot plant cultivars as well as identify their genetic relationships.

scholarly journals Genetic Relationships of Pyrus Species and Cultivars Native to East Asia Revealed by Randomly Amplified Polymorphic DNA Markers

2002 ◽  
Vol 127 (2) ◽  
pp. 262-270 ◽  
Author(s):  
Yuanwen Teng ◽  
Kenji Tanabe ◽  
Fumio Tamura ◽  
Akihiro Itai
Keyword(s):  
East Asia ◽  
Clustering Analysis ◽  
Rapd Markers ◽  
Rapd Analysis ◽  
Genetic Relationships ◽  
Randomly Amplified Polymorphic Dna ◽  
Pyrus Calleryana ◽  
Chinese White ◽  
Species Specific ◽  
Kochi Prefecture

A total of 118 Pyrus sp. (pear) and cultivars native mainly to east Asia were subjected to randomly amplified polymorphic DNA (RAPD) analysis to evaluate genetic variation and relationships among the accessions. Two hundred fifty RAPD markers were scored from 20 decamer primers. RAPD markers specific to species were identified. Clustering analysis revealed two divisions: one comprising cultivars of P. communis L., and the other including all accessions of Pyrus native to east Asia. The grouping of the species and cultivars by RAPD data largely agrees with morphological pear taxonomy. However, some noted incongruence existed between two classification methods. Pyrus calleryana Dcne. clustered together with P. koehnei Schneid., P. fauriei Schneid. and P. dimorphophylla Makino. Pyrus betulaefolia Bge. clustered with P. ×hopeiensis Yu and P. ×phaeocarpa Rehd. A noncultivated clone of P. aromatica Kikuchi et Nakai grouped with P. aromatica cultivars. Pyrus hondoensis Nakai et Kikuchi and cultivars of P. ussuriensis Max. formed a single group. Some accessions from Korea (named Korean pear) had species-specific RAPD markers and comprised an independent group. Most of the Chinese white pears clustered together with most of the Chinese sand pears. Based on the present results, the new nomenclature P. pyrifolia var. sinensis (Lindley) Teng et Tanabe for Chinese white pear was suggested. Most accessions of Japanese pears fell into one main group, whereas pear cultivars from Kochi Prefecture of Japan subclustered with some Chinese sand pears and one accession from Korea. Our results infer that some local Japanese pear cultivar populations may have been derived from cultivars native to Kochi Prefecture in Shikoku region, and that the latter may have been introduced from ancient China and/or Korea.

scholarly journals Use RAPD Technique for Characterization of Genotypical Variability of Maize Germplasm from SCDA Turda

2010 ◽  
Vol 67 (1) ◽  
Author(s):  
Rodica POP ◽  
Doru PAMFIL ◽  
Monica HÂRŢA ◽  
Ioan HAŞ ◽  
Iulia POP
Keyword(s):  
Rapd Markers ◽  
Rapd Analysis ◽  
Genetic Relationships ◽  
Germplasm Collection ◽  
Genetic Distances ◽  
Maize Germplasm ◽  
Upgma Dendrogram ◽  
Maize Genotypes ◽  
Polymorphic Bands

Genetic analysis with RAPD markers has been extensively used to determine diversity among maize genotypes. The aim of the present study was to estimate genetic relationships among 70 genotypes, provided from SCDA Turda Cluj germplasm collection. RAPD analysis was performed with 14 decamer primers. These primers generated, among the studied genotypes, a number of polymorphic bands comprised between 13 bands (OPA 04) and 7 bands (OPAL 20). The highest numbers of polymorphic bands were obtained with primer OPA 04, respectively 13 bands, following by OPO 12 (12 polymorphic bands), OPAB 11 and OPA 17 (11 polymorphic bands). Lowest number was obtained with primer OPAL 20, respectively 7 polymorphic bands. Genetic distances were established using Nei-Li coefficient and UPGMA dendrogram was constructed with RAPDistance 1.04 software. The built dendrogram shows phylogenetic relationships between genotypes analyzed.

scholarly journals Identification of Poinsettia Cultivars Using RAPD Markers

HortScience ◽  
1997 ◽  
Vol 32 (1) ◽  
pp. 122-124 ◽  
Author(s):  
Jing-Tian Ling ◽  
Roger Sauve ◽  
Nick Gawel
Keyword(s):  
Cluster Analysis ◽  
Rapd Markers ◽  
Genetic Relationships ◽  
The Other ◽  
Euphorbia Pulcherrima ◽  
Randomly Amplified Polymorphic Dna ◽  
Upgma Cluster Analysis ◽  
Leaf Tissues ◽  
Components Analysis

Randomly amplified polymorphic DNA (RAPD) techniques were used to compare the DNA from leaf tissues of nine commercial poinsettia (Euphorbia pulcherrima Wild ex Klotzsch) cultivars. Amplification occurred in 57 out of 60 (95%) tested primers. Nine primers that revealed polymorphisms among cultivars were selected for further evaluation. Forty-eight RAPD bands were scored from these primers, and 33 (69%) were polymorphic. All tested cultivars could be discriminated with seven bands generated from primers OPB7 and OPC13. Results of a UPGMA cluster analysis and principal components analysis placed the nine cultivars into two groups: one group consisted of `Jingle Bells', `Supjibi', and `V-17 Angelika', the other of `V-14 Glory', `Red Sails', `Jolly Red', and `Freedom'. `Lilo Red' and `Pink Peppermint' belonged to the latter group, but were relatively distant from other cultivars in that group. These results indicate that RAPDs are efficient for identification of poinsettia cultivars and for determination of the genetic relationships among cultivars.

scholarly journals RAPD analysis of genetic relatedness among selected quince (Cydonia oblonga Mill.) accessions from different parts of Turkey

2011 ◽  
Vol 38 (No. 4) ◽  
pp. 134-141 ◽  
Author(s):  
S. Bayazit ◽  
B. Imrak ◽  
A. Küden ◽  
M. Kemal Güngör
Keyword(s):  
Rapd Markers ◽  
Genetic Relatedness ◽  
Geographical Origin ◽  
Rapd Analysis ◽  
Genetic Relationships ◽  
Randomly Amplified Polymorphic Dna ◽  
Cydonia Oblonga ◽  
A Minor ◽  
Almost All ◽  
Different Parts

Quince (Cydonia oblonga Mill.) is a minor fruit crop, which is primarily used for marmalade, jam, sauce and as rootstocks for pears. Different cultivated and local quince genotypes are grown in almost all parts of Turkey for fruit usage. In this study, randomly amplified polymorphic DNA (RAPD) technology was used to study the genetic relationships among 13 quince accessions selected from different parts of Turkey. Thirty decamer primers were used and 14 of them did not produce any polymorphism. The remaining 16 primers ranged in their amplification fragments between one (P-402, P-437, OPA 10, OPA 16, OPA 18 and OPA-19) and five (OPA-06 and OPA-07). The size of fragments varied from 100 to 1500 bp. Similarity values among the studied genotypes ranged between 0.483 and 0.925. The resulting dendrogram clustered into two groups (0.69 similarity value) based on evaluation of genetic similarities and differences. The results suggest that RAPD analysis could be used to distinguish and determine genetic variation among quince accessions. Also, the obtained clustering based on RAPD markers agreed to some extent with the geographical origin of the studied set of quince accessions.

scholarly journals Molecular Characterization of Vitex agnus-castus L. (Verbenaceae) Populations Grown in Aydin, Turkey

2019 ◽  
Vol 11 (2) ◽  
pp. 218-221
Author(s):  
Emre SEVİNDİK ◽  
Serkan CANER ◽  
Mahmut COŞKUN
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Analysis ◽  
Genomic Dna ◽  
Rapd Markers ◽  
Genetic Relationships ◽  
Genetic Distances ◽  
Purple Flower ◽  
A Value ◽  
Pink Flower ◽  
Agnus Castus

In this study, we performed a genetic diversity analysis using RAPD markers for some Vitex agnus-castus populations grown in Aydin, Turkey. Total genomic DNA isolation from the leaves of Vitex agnus-castus was performed using a commercial kit. Seven RAPD primers (OPA-02, OPA-05, OPA-13, OPA-15, OPA-16, OPA-18, OPA-20) were used to determine genetic diversity among populations. Polymerase Chain Reaction (PCR) was performed with all genomic DNA samples and primers. PCR products were run in agarose gel electrophoresis and visualized under UV light. The amplified products were scored as bands (1) and no bands (0) for all gel images and their matrix files were generated. A total of 36 characters were obtained from the primers. Phylogenetic relationships and genetic distances between the cultivars were calculated by using the PAUP* (Phylogenetic Analysis Using Parsimony and other methods) program. According to PAUP analysis, the closest genetic distances were between Çine pink flower and Çakmar purple flower, and Çakmar pink flower and Çakmar purple flower populations with a value of 0.05556; and the greatest genetic distance was between Çakmar pink flower and Köşk purple flower populations with a value of 0.36111. In the phylogenetic analysis obtained using UPGMA algorithms, the phylogenetic tree consisted of four groups. The results suggest that RAPD markers are useful tools for determining genetic relationships among Vitex agnus-castus genotypes.

scholarly journals Genetic relationships among species of the genus Bothrops based on RAPD markers

2005 ◽  
Vol 48 (3) ◽  
pp. 359-365 ◽  
Author(s):  
Felipe Grazziotin ◽  
Sergio Echeverrigaray
Keyword(s):  
Phylogenetic Analysis ◽  
Rapd Markers ◽  
Genetic Relationships ◽  
Group Iii ◽  
Group I ◽  
Group Ii ◽  
Similarity Groups

The genetic relationships among 11 species of Bothrops found in Brazil were determined using random amplified fragment (RAPD) information. A total of 239 amplified bands were scored using 20 aleatory primers. Most of the bands (88.7%) were polymorphic. Phylogenetic analysis of the data determined three similarity groups within Bothrops: Group I- B. alternatus, B. neuwiedi, B. cotiara, and B. jararacusu; Group II- B. insularis, B. jararaca, and B. erythromelas; and Group III- B. moojeni, B. leucurus, and B. atrox. These groups coincided with those obtained by other methods, indicating that RAPD's could be a useful tool for the evaluation of genetic relationships at the interspecific level.

scholarly journals Characterization and Identification of Spanish Olive Germplasm by Means of RAPD Markers

HortScience ◽  
2004 ◽  
Vol 39 (2) ◽  
pp. 346-350 ◽  
Author(s):  
A. Belaj ◽  
I. Trujillo ◽  
D. Barranco ◽  
L. Rallo
Keyword(s):  
Rapd Markers ◽  
Cultivar Identification ◽  
Randomly Amplified Polymorphic Dna ◽  
Olive Cultivar ◽  
Banding Patterns ◽  
Olive Cultivars ◽  
Polymorphic Bands

Thirteen randomly amplified polymorphic DNA (RAPD) primers were assayed in 82 Spanish olive cultivars of economical interest. A total of 82 bands were scored giving an average of 6.3 bands per primer. A total of 4 (OPA-01) to 10 bands (OPA-19) was amplified, while the number of polymorphic fragments ranged from 2 (OPK-07) to 9 (OPA-19) with a mean of 5.7 polymorphic bands per primer. A total of 89% of the amplification products (73 bands) were polymorphic. The 13 primers yielded 184 banding patterns (14.9 per primer). The number of banding patterns per primer ranged from 4 (OPK-07) to 39 (OPA-19). Fifty-three unique banding patterns were found, the majority of them resulted from different combinations of polymorphic bands. The combination of only five primers OPA-19, OPF-06, OPX-01, OPX-03, and OPI-12, allowed identification of all the cultivars. Seventy-four cultivars (90%) were identified only by the combination of the first four primers. The addition of the fifth primer (OPI-12) was necessary for the identification of the eight remaining cultivars (10%). The ordination of the primers according to their practical discriminating capacity in this study was: OPA-19 > OPF-06 > OPX-01 > OPX-03 > OPI-12. Hence RAPD markers are recommended for olive fingerprinting in order to generate a database for olive cultivar identification.

scholarly journals 660 PB 072 IDENTIFICATION OF GENETIC VARIABILITY AMONG SPECIES AND CULTIVARS OF PENSTEMON USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPDS)

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 527d-527
Author(s):  
Guenhwa Jung ◽  
Dale T. Lindmen ◽  
Dermot P. Coyne
Keyword(s):  
Genetic Variability ◽  
Principle Component Analysis ◽  
Rapd Markers ◽  
Genetic Relationships ◽  
Random Amplified Polymorphic Dna ◽  
Component Analysis ◽  
Dna Fingerprints ◽  
Principle Component ◽  
Polymorphic Bands

Eight species and 57 selections/cultivars of Penstemon were compared for genetic variability using Random Amplified Polymorphic DNAs (RAPDs). The RAPD technique was used to help understand the genetic relationships in species and cultivars in the genus Penstemon. Ten RAPD primers (from Operon) were screened to identify polymorphisms among these eight species and 57 selections. More than 100 RAPD polymorphic bands were obtained. A principle component analysis was used to study genetic relationships. Variation among species was greater than variation among selections/cultivars within species. RAPD markers distinguished differences between most cultivars tested. DNA fingerprints generated by RAPDs should be useful to distinguish cultivars of Penstemon, as well as to assist in determining genetic relationships between species.

scholarly journals Identification of Pecan Cultivars and Their Genetic Relatedness as Determined by Randomly Amplified Polymorphic DNA Analysis

2001 ◽  
Vol 126 (4) ◽  
pp. 474-480 ◽  
Author(s):  
Patrick J. Conner ◽  
Bruce W. Wood
Keyword(s):  
Genetic Similarity ◽  
Rapd Markers ◽  
Dna Analysis ◽  
Genetic Relatedness ◽  
Genetic Relationships ◽  
Genetic Distances ◽  
Similarity Coefficient ◽  
Carya Illinoinensis ◽  
Randomly Amplified Polymorphic Dna ◽  
Average Value

Genetic variation among pecan [Carya illinoinensis (Wangenh.) C. Koch] cultivars was studied using randomly amplified polymorphic DNA (RAPD) markers. Using a combination of primers, a unique fingerprint is presented for each of the pecan genotypes studied. The genetic relatedness between 43 cultivars was estimated using 100 RAPD markers. Genetic distances, based on the similarity coefficient of Nei & Li, varied from 0.91 to 0.46, with an average value of 0.66 among all cultivars. The phenetic dendrogram developed from cluster analysis showed relatively weak grouping association. However, cultivars with known pedigrees usually grouped with at least one of the parents and genetic similarity estimates appear to agree with known genetic relationships.

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