agnus castus
Recently Published Documents





2021 ◽  
Vol 0 (0) ◽  
Ceren Kımna ◽  
Tuğçe Fafal

Abstract Objectives In this study, we aim at deciphering the phenolic content of Vitex agnus-castus L. leaf and fruit extracts prepared with different methods and relate it to their antioxidant activity. Methods In this study, phenolic compounds and the antioxidant potential of the ethanol fruit and leaf extracts of V. agnus-castus L. (Chaste tree) were evaluated spectrometrically. Furthermore, selected polyphenols, i.e., chlorogenic acid and rutin, were determined by the HPLC-DAD method qualitatively and quantitatively. Results The results obtained from leaf and fruit extracts were compared with a commercial product (CP) containing the fruit extract of V. agnus-castus. Leaf extract was found to be richer in flavonoids when compared to the fruit counterparts. Accordingly, they also showed higher antioxidant activity. Conclusions Extracts prepared here can be considered as promising antioxidant agents for future therapeutic formulations.

2021 ◽  
Vol 49 (4) ◽  
pp. 12292
Alireza IRANBAKHSH ◽  
Mostafa EBADI

Each environmental factor is able to change the way genes are expressed. Application of nanoparticles also affects the expression of different genes in plants. The aim of this study was to investigate the effect of three different concentration of titanium dioxide nanoparticles, TiO2 (zero, 200 and 800 micrograms per milliliter) on the relative expression of catalase, P450, SOD, diTDS and WRKY genes in Vitex plant leaf tissue using qRT- PCR. Plant cultivation was carried out in 2018 in the greenhouse of Islamic Azad University of Mashhad. The experiment was arranged as completely random design with 5 replications. XRD measurements showed that applied TiO2 nanoparticles were in the form of anatase. Statistical analysis of gene expression in treated leaves of Vitex plant with TiO2 nanoparticles showed that this nanoparticle significantly affected the expression of catalase, P450, SOD, diTPS and WRKY genes. A concentration of 800 micrograms per milliliter of TiO2 nanoparticle increased the expression of catalase, P450, SOD and WRKY genes and decreased the expression of diTPS gene. In contrast, concentrations of 200 micrograms per milliliter only increased the expression of catalase and WRKY genes. The expression of the diTPS gene under treatments of 200 and 800 micrograms per liter of TiO2, compared with control, decreased by 2.1 and 0.46, respectively. Overall, the nanoparticle was able to influence the expression of genes in the biosynthetic pathway of terpenoids, as well as the plant's antioxidant enzymes, depending on the concentration of nanoparticles.

Blood ◽  
2021 ◽  
Vol 138 (Supplement 1) ◽  
pp. 1043-1043
Susan Halimeh ◽  
Sylvia Von Mackensen ◽  
Lina Lourak ◽  
June Schwarzbach ◽  
Manuela Siebert

Abstract Background: Heavy menstrual bleeding (HMB) is a common gynaecological problem and is the reason for 18-30% of gynaecological visits. In a European study in 4,502 women, 27.2% were diagnosed with HMB (Fraser et al., 2015). On the other hand, HMB is often associated with bleeding disorders (Shankar et al., 2004). The definition of HMB has different perspectives; from a subjective perspective HMB is defined as e excessive menstrual blood loss impacting on women's physical, social, emotional and/or mental quality of life, whereas from an objective perspective it is defined as excessive blood loss >80 ml per cycle (Munro et al., 2012). There are different approaches for treating HMB such as the administration of non-steroidal drugs, Desmopressin, herbal Vitex Agnus Castus (VAC), Tranexamic acid (TXA) or a hormonal therapy; moreover, HMB can also be treated surgically. The aim of this study was to test the efficacy of the specific HMB management used in our center: Women with HMB are treated usually with VAC, which optimizes the relation of estrogen to progesterone in the female body (Yavarikia et al., 2013). VAC can be used without using any hormones (Shahnazi et al, 2016), providing a treatment with a low impact on women's bodies. Additionally, TXA is given during the menstruation due to its antifibrinolytic effect. Furthermore, the "Pictural Blood-Loss-Assessment- Chart" (PBAC Score) is administered to analyze the menstrual blood loss. Methods: Two ad hoc patient-reported questionnaires were developed to test the efficacy of the HMB management in our center The baseline questionnaire includes the following aspects: menarche, duration, regularity, number of sanitary products, pain level, medical background and family history. During the first visit in our center women with HMB filled in the baseline questionnaire. In addition, laboratory tests are done including a comprehensive coagulation test and the examination of iron and hemoglobin levels. Moreover, we presented an application called "My Flow Score" to the patients, which calculates the PBAC score as a result of all entered sanitary products women use during their period. Women are prescribed VAC and/or TXA for the management of their HMB. After four months patients are scheduled for a follow up appointment. At that time they complete the follow-up questionnaire including the following aspects: compliance with the medication or the reason for the non-compliance, health complaints due to the medication, duration of the period, improvement, pain level, PBAC score if the patient did use the APP "My Flow Score" or if not the number of sanitary products. A blood test was taken and the HMB management was adapted to the patients' needs. Results: So far, 100 women with HMB with a median age of 14 years (range 9-50) were enrolled in our study. They had their menarche with a median age of 12 years (range 8-17). Diagnosis of women ranged from iron or folic acid deficiency to different forms of bleeding disorders; with the majority suffering from von Willebrand disease (43/100) followed by iron deficiency (14/100). One fifth of the patients had more than one diagnosis. 1/3 of patients received a treatment for the HMB previously, mainly contraceptives, TXA or VAC. 49% reported anomalies with regard to previous other bleeding; 25.8% had increased hematomas and 19.6% recurrent epistaxis. Most of them reported anomalies already in the family (60.2%). Before treatment women had a median PBAC Score of 169 (range 77-800) and reported a median pain level of 6.5 during menstruation on a scale ranging from 1 (low pain) to 10 (extreme pain). 87/100 women received VCA, of these 75.4% regularly and 85/100 women received TXA, of these 84.6% regularly. 81% of them received a combination of both medicines. Compared to before 28.8% of patients reported shorter duration of menstruation and reduced bleeding with treatment, 11.9% did not experience any improvement; the PBAC score decreased significantly (p<.0001). Conclusions: Although the type of diagnosis in women suffering from HMB had a great variation, these patients had a high disease burden with a relatively high level of pain and a high loss of blood assessed with the PBAC score. Thanks to a combination of VAC and TXA the disease burden in these women could be reduced, especially for the time of menstruation and amount of bleeding. VAC proved to be highly accepted by women due to its low treatment burden. Disclosures Von Mackensen: University Medical Centre Hamburg-Eppendorf: Current Employment; Sobi: Consultancy, Membership on an entity's Board of Directors or advisory committees, Research Funding, Speakers Bureau; Sanofi: Consultancy; Novo Nordisk: Consultancy; Biomarin: Speakers Bureau; CSL Behring: Speakers Bureau; Chugai/Roche: Membership on an entity's Board of Directors or advisory committees.

Rotimi Sunday Ajani ◽  
Nyerhovwo Abijah Akpovwovwo ◽  
Theophilus A. Jarikre ◽  
Benjamin O. Emikpe

Objective: The liver is the major organ of detoxification of ingested materials such as food, beverages and drugs, thus it is prone to toxicity with attendant pathologies. We studied the ability of Vitex agnus castus plant extract to ameliorate the biochemical and structural alterations in Wistar rats with carbon tetrachloride-induced liver injury. Methods: Forty adult male Wistar rats were allotted into eight equal groups. Group1 was normal control (NC); Group 2 Liver injury without extract (LI). The remaining six groups were paired composite group of varying dosage of the plant extract (200 mg/kg, 400 mg/kg and 600 mg/kg). Only one member of each pair had induced liver injury. Consequently, the groups were Low extract without liver injury (LE), Low extract with liver injury (LEL); Medium extract without liver injury(ME)  Medium extract with liver injury (MEL) and High extract without liver injury (HE),High extract with liver injury (HEL).The biochemical parameters evaluated were the liver function test {Total protein plus globulin and albumen fractions; liver enzymes- alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase}. Oxidative stress was evaluated by measuring the activities of antioxidants namely; Superoxide dismutase (SOD), Catalase, Malondialdehyde (MDA) and Glutathione Peroxidase (GPx).Histology of the harvested liver specimens was used to assess the structural alterations. Results: The mean liver weight was highest in the HEL group and significantly different from that of its control (HE) and NC.The mean serum total protein of all the groups were significantly higher than that of the NC.The mean aspartate amino transferase levels of the LEL, ME and MEL groups were significantly lower than that of the control while that of the HEL was significantly higher than those of the LEL and MEL. The results of alanine amino transferase were similar to those of aspartate amino transferase. The alkaline phosphatase levels in all the experimental groups were significantly depressed when compared with the control. Amongst the experimental groups, the serum alkaline phosphatase level was significantly raised than those of the LEL, and MEL groups. The glutathione (GSH) activities of LE, HE and HEL were significantly lower. While the glutathione peroxidase (GPX) activity of the control was significantly lower to those of groups LEL, ME, MEL and HEL.Histopathology of the liver showed preservation of the liver architecture with normal hepatocytes in all the groups. Conclusion: The ethanolic extract of Vitex agnus castus was able to reduce the severity of carbon tetrachloride induced liver injury in wistar rats.

2021 ◽  
Vol 77 (8) ◽  
pp. 491-502
Anna Magiera ◽  
Aleksandra Prokop ◽  
Mateusz Gieleta ◽  
Monika Olszewska

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Assia Berrani ◽  
Ilias Marmouzi ◽  
Abdelhakim Bouyahya ◽  
Mourad Kharbach ◽  
Maha El Hamdani ◽  

Vitex agnus-castus is a medicinal plant of the Verbenaceae family, widely used in traditional medicine. This study is aimed at investigating the functional variability of phenolic compounds in different parts (leaves, stems, flowers, roots, and seeds) of Vitex agnus-castus methanolic extracts and at assessing their in vitro antidiabetic, antioxidant, and antibacterial activities. The results of HPLC-DAD-QTOF-MS indicated the presence of 25 phenolic compounds with a remarkable variability between plant parts; high levels were registered in chlorogenic, vanillic, 3,4-dihydroxybenzoic, and 3-hydroxybenzoic acids; hesperidin; and luteolin. V. agnus castus fruits and stems presented higher antioxidant activities. The extracts inhibited the growth of five pathogenic bacteria with MIC values documented between 7.81 and 31.25 mg/mL. In vitro antihyperglycemic effect revealed higher effect in flowers (2921.84 μg/mL) and seeds (2992.75 μg/mL) against α-glucosidase and of leaves (2156.80 μg/mL) and roots (2357.30 μg/mL) against α-amylase. The findings of this showed that V. agnus castus is a promising source for antidiabetic bioactive compounds. However, further investigations regarding the evaluation of in vivo antidiabetic effects of these compounds are needed.

Sign in / Sign up

Export Citation Format

Share Document