Wether bioassay of annual pasture legumes. III. The oestrogenic potency of dry subterranean clover pastures, and of leaf blade and petiole in the green state

1965 ◽  
Vol 16 (1) ◽  
pp. 23 ◽  
Author(s):  
CM Francis ◽  
AJ Millington
Keyword(s):  
Leaf Blade ◽  
Subterranean Clover ◽  
Late Summer ◽  
Green Leaf ◽  
Green State ◽  
Pasture Legumes ◽  
Measurable Activity

Late summer pastures of nine strains of subterranean clover had very low oestrogenic activity as assessed by wether teat length bioassay. Only the Dwalganup variety had measurable activity which may have been due mainly to moderate levels of coumestrol in the various dry plant fractions. The activity of green leaf blade of the Yarloop strain was about nine times as great as that of the petioles. Possible agronomic implications of this finding are discussed.

Flower initiation in pasture legumes. III. Flower initiation in Medicago tribuloides Desr. and other annual medics.

1955 ◽  
Vol 6 (2) ◽  
pp. 258 ◽  
Author(s):  
Y Aitken
Keyword(s):  
Low Temperature ◽  
Subterranean Clover ◽  
Late Summer ◽  
Flower Initiation ◽  
Commercial Variety ◽  
Barrel Medic ◽  
Pasture Legumes ◽  
Annual Medics ◽  
Long Photoperiod

Observations on barrel medic (Medicago tribuloides Desr., commercial variety) sown throughout the year in the field showed that the flower initiation of the winter sowings was the most rapid and that of the late summer sowings the most delayed. This delay was due to the absence of sufficiently low temperature following germination. Low temperature and long photoperiod accelerate flower initiation in barrel medic as in subterranean clover. Five types of annual medic that persist under Mallee conditions are similar in their flower initiation character to that of the "second-early" varieties of subterranean clover.

Variability of in vitro ruminal fermentation and methanogenic potential in the pasture legume biserrula (Biserrula pelecinus L.)

2013 ◽  
Vol 64 (4) ◽  
pp. 409 ◽  
Author(s):  
Bidhyut Kumar Banik ◽  
Zoey Durmic ◽  
William Erskine ◽  
Phillip Nichols ◽  
Kioumars Ghamkhar ◽  
...  
Keyword(s):  
Core Collection ◽  
Volatile Fatty Acids ◽  
Germplasm Collection ◽  
Red Clover ◽  
Subterranean Clover ◽  
Ruminal Fermentation ◽  
Rumen Microbes ◽  
Pasture Legumes ◽  
Pasture Legume

Biserrula (Biserrula pelecinus L.) is an important annual pasture legume for the wheatbelt of southern Australia and has been found to have lower levels of methane output than other pasture legumes when fermented by rumen microbes. Thirty accessions of the biserrula core germplasm collection were grown in the glasshouse to examine intra-specific variability in in vitro rumen fermentation, including methane output. One biserrula cultivar (Casbah) was also grown at two field locations to confirm that low methanogenic potential was present in field-grown samples. All of the biserrula accessions had significantly reduced methane [range 0.5–8.4 mL/g dry matter (DM)] output compared with subterranean clover (28.4 mL/g DM) and red clover (36.1 mL/g DM). There was also significant variation in fermentability profiles (except for volatile fatty acids) among accessions of the core collection. Methanogenic potential exhibited 86% broad-sense heritability within the biserrula core collection. The anti-methanogenic and gas-suppressing effect of biserrula was also confirmed in samples grown in the field. In conclusion, biserrula showed variability in in vitro fermentation traits including reduced methane production compared with controls. This bioactivity of biserrula also persists in the field, indicating scope for further selection of biserrula as an elite methane-mitigating pasture.

Performance and weed-suppressive potential of selected pasture legumes against annual weeds in south-eastern Australia

2019 ◽  
Vol 70 (2) ◽  
pp. 147 ◽  
Author(s):  
Sajid Latif ◽  
Saliya Gurusinghe ◽  
Paul A. Weston ◽  
William B. Brown ◽  
Jane C. Quinn ◽  
...  
Keyword(s):  
Subterranean Clover ◽  
Weed Suppression ◽  
Weed Species ◽  
Mixed Stands ◽  
South Eastern ◽  
Pasture Legumes ◽  
Eastern Australia ◽  
Weed Suppressive Ability ◽  
Weed Infestation ◽  
South Eastern Australia

Mixed farming systems have traditionally incorporated subterranean clover (Trifolium subterraneum L.) and lucerne (Medicago sativa L.) as key components of the pasture phase across south-eastern Australia. However, poor adaptation of subterranean clover to acidic soils, insufficient and inconsistent rainfall, high input costs, soil acidification and the emergence of herbicide-resistant weeds have reduced efficacy of some traditional clover species in recent years. To overcome these challenges, numerous novel pasture species have been selectively improved and released for establishment in Australia. Despite their suitability to Australian climate and soils, limited knowledge exists regarding their weed-suppressive ability in relation to establishment and regeneration. Field trials were therefore conducted over 3 years in New South Wales to evaluate the suppressive potential of selected pasture legume species and cultivars as monocultures and in mixed stands against dominant annual pasture weeds. Pasture and weed biomass varied significantly between pasture species when sown as monocultures, but mixtures of several species did not differ with regard to establishment and subsequent weed infestation. Arrowleaf clover (T. vesiculosum Savi.) and biserrula (Biserrula pelecinus L.) cv. Casbah showed improved stand establishment, with higher biomass and reduced weed infestation compared with other pasture species. Generally, weed suppression was positively correlated with pasture biomass; however, yellow serradella (Ornithopus compressus L.) cv. Santorini exhibited greater weed suppression than other pasture legumes while producing lower biomass, thereby suggesting a mechanism other than competition for resources affecting weed-suppressive ability. Over the period 2015–17, arrowleaf clover and biserrula cv. Casbah were generally the most consistent annual pasture legumes with respect to yearly regeneration and suppression of annual pasture weed species.

In vitroreproduction in the annual pasture legumes subterranean clover (Trifolium subterraneumL.) and French serradella (Ornithopus sativusBrot.)

2015 ◽  
Vol 71 (1) ◽  
pp. 79-89 ◽  
Author(s):  
M. Castello ◽  
K. Stefanova ◽  
P. G. H. Nichols ◽  
B. J. Nutt ◽  
C. K. Revell ◽  
...  
Keyword(s):  
Subterranean Clover ◽  
Pasture Legumes

Long-term persistence of subterranean clover Trifolium subterraneum L.) cultivars at three sites in south-western Victoria

1997 ◽  
Vol 37 (5) ◽  
pp. 531
Author(s):  
S. G. Clark ◽  
J. Li ◽  
A. M. Johnson ◽  
G. N. Ward ◽  
J. F. Chin
Keyword(s):  
Perennial Grass ◽  
Subterranean Clover ◽  
Late Summer ◽  
Trifolium Subterraneum ◽  
Contamination Level ◽  
Black And White ◽  
Small Plot ◽  
Black Seeds ◽  
Western Victoria ◽  
Annual Weeds

Summary. The seed banks of 6 subterranean clover (Trifolium subterraneum L.) cultivars were sampled at 3 abandoned cultivar evaluation experiments in south-western Victoria (Hamilton, Macarthur and Timboon). Two were var. yanninicum (Trikkala and Larisa) and 4 were var. subterraneum (Denmark, Goulburn, Karridale and Leura). Seed was sampled in late summer–early autumn 1993, between 6 and 10 years after the sites were established and between 3 and 7 years after the sites were abandoned. The collected seed was separated into black and white seed, and a sample of the black seed was grown in a glasshouse and identified as either belonging to the sown cultivar or belonging to another cultivar/genotype. All white seed was assumed to belong to the sown cultivar. The aim was to determine if these widely used cultivars were persisting under farm management conditions and competition from perennial grass, other subterranean clover and annual weeds. With some variation between sites all cultivars were found to be persisting satisfactorily. Over the 3 sites, white-seeded cultivars averaged 460 kg/ha and 6640 seeds/m2; black-seeded cultivars averaged 260 kg/ha and 5590 seeds/m2. Contamination with other subterranean clover cultivars/ecotypes was generally low, except at Macarthur where the white-seeded cultivars were heavily contaminated (Trikkala 39% pure; Larisa 57% pure) with black seeds highlighting the poor adaptation of var. yanninicum to light soil types. Of the black seeds, over the 3 sites, average contamination level was 13% and ranged from 41% (Goulburn at Macarthur) to 1% (Leura at Timboon). The study indicates that in south-western Victoria, all the recently released cultivars are likely to persist and be productive, and that the small-plot evaluation techniques used to identify potential new cultivars are likely to be adequate if replicated in space and if the clovers are sown in mixed swards with perennial grass.

Effect of perennial pasture species on surface soil moisture and early growth and survival of subterranean clover (Trifolium subterraneum L.) seedlings

1997 ◽  
Vol 48 (5) ◽  
pp. 683 ◽  
Author(s):  
B. S. Dear ◽  
P. S. Cocks
Keyword(s):  
Mineral Soil ◽  
Subterranean Clover ◽  
Late Summer ◽  
Soil Surface ◽  
Trifolium Subterraneum ◽  
Annual Grass ◽  
Growth And Survival ◽  
Eastern Australia ◽  
Standing Dead ◽  
Clover Seed

Subterranean clover seedling numbers and growth in swards containing 1 of 5 perennial pasture species [phalaris (Phalaris aquatica) cv. Sirolan, cocksfoot (Dactylis glomerata) cv. Currie, lucerne (Medicago sativa) cv. Aquarius, wallaby grass (Danthonia richardsonii) cv. Taranna, and lovegrass (Eragrostis curvula) cv. Consol] were compared with those in typical annual pastures and pure clover swards in the wheatbelt of eastern Australia. Presence of a perennial species or the volunteer annual grass (Eragrostis cilianensis) increased the rate of drying of the soil surface (0–5 cm) after late February and May rain, compared with subterranean clover swards. Perennials differed in the rate they dried the soil surface, with the more summer-active lucerne and consul lovegrass drying the profile more rapidly than phalaris. The amount of water in the surface 5 cm, 6 days after the rainfall event on 27–28 February, was strongly negatively correlated (r = –0·75, P < 0·01) with the amount of green perennial biomass, but not related to standing dead material or surface residues. Where perennials were present, a smaller proportion (2–4%) of the clover seed pool produced seedlings in response to late summer rain, compared with pure clover swards (18%). A higher proportion of the seed pool produced seedlings (19–36%) following rain in late autumn but there was no difference between species. The more summer-active perennials (cocksfoot, danthonia, and lucerne) markedly depressed the survival of emerged clover seedlings following both germinations. Of the seedlings that emerged in early March, the proportion remaining by 29 March was 57% in phalaris, 21% in lucerne, 13% in danthonia, and 1% in cocksfoot, compared with a 78% increase in seedlings in pure subterranean clover swards. By 15 May, all perennials had <2 clover seedlings/m2 surviving, compared with 37 in the annual pasture and 964 plants/m2 in pure subterranean clover. Following the May germination, the highest proportion of emerged seedlings surviving until 29 May was in the phalaris swards (40%) and least in the cocksfoot and danthonia swards (2–4%). Presence of a perennial or annual grass decreased (P < 0·05) relative water content of clover seedlings on 15 March from 74% in pure clover swards, to 48% in annual pasture, 34% in phalaris, and 29% in lucerne swards. Clover seedlings growing in pure subterranean swards on 15 March (17 days after germinating rain) were 4 times larger than those in lucerne and twice as large as those in either phalaris or annual pasture. Seed size did not differ between treatments, but available mineral soil nitrogen was significantly higher (P < 0·001) in pure subterranean clover swards (32 mg N/g) compared with perennials (3–13 mg N/g). Strategies such as heavy grazing in late summer to reduce green biomass of the perennials or sowing the perennials at lower densities may reduce the adverse effects that perennials have on subterranean clover seedlings in these drier environments.

The transference of nitrogen from pasture legumes to an associated grass under several systems of management in pot culture

1965 ◽  
Vol 16 (6) ◽  
pp. 915 ◽  
Author(s):  
JR Simpson
Keyword(s):  
White Clover ◽  
Subterranean Clover ◽  
Experimental Period ◽  
The Other ◽  
Temporary Increase ◽  
Winter Period ◽  
Pasture Legumes ◽  
Perennial Legumes ◽  
Intact Root ◽  
Pot Culture

The extent of underground transference of nitrogen from three pasture legumes, each growing in association with a grass, has been studied in pot culture under several systems of management during the first 12–18 months after sowing. The three legumes performed quite differently. Subterranean clover did not release any nitrogen until senescence and then produced a rapid transference. White clover was competitive for nitrogen until the autumn-winter period. Lucerne released nitrogen gradually over the whole experimental period. Frequent defoliation of the legumes reduced competition for nitrogen by white clover but also reduced transference from the other legumes. Killing the perennial legumes produced only a small temporary increase in transference. Wilting and temporary drying treatments also reduced the transference. Thus there was no evidence that the nitrogen transference from lucerne was due to a shedding or decay of nodules induced by defoliation; it could equally well be due to direct excretion of nitrogen from the intact root system. The significance of the results in pasture establishment in infertile areas is discussed.

Flower initiation in pasture legumes. I. Factors affecting flower initiation in Trifolium subterraneum L.

1955 ◽  
Vol 6 (2) ◽  
pp. 211 ◽  
Author(s):  
Y Aitken
Keyword(s):  
Low Temperature ◽  
Subterranean Clover ◽  
Trifolium Subterraneum ◽  
Cold Period ◽  
Effect Of Temperature ◽  
Flower Initiation ◽  
Temperature Level ◽  
Factors Affecting ◽  
Pasture Legumes ◽  
Precise Knowledge

The value of the annual legume Trifolium subterraneum L. (subterranean clover) in Australian agriculture warrants more precise knowledge of factors affecting flowering and prolific seeding. The effect of temperature and photoperiod on flower initiation in early and later flowering varieties has been investigated in an effort to determine the geographical limits of the use of subterranean clover in Australia. At any time of sowing, the length of the growing season of a variety depends greatly on the variety's response to the temperature level and to the photoperiod of the first few weeks after germination. In all varieties of subterranean clover so far examined flower initiation is accelerated by a period of low temperature. In the later varieties, flower initiation is prevented by an insufficient period of low temperature. The length of the necessary cold period is shortened under longer photoperiod. Early varieties are early flowering because they do not require so long a cold period or so low a temperature as late varieties.

Manganese toxicity in Trifolium balansae, T. resupinatum, T. subterraneum, Medicago murex, M. polymorpha, M. sativa, Lotus pedunculatus, and Ornithopus compressus: relative tolerance and critical toxicity concentrations

1995 ◽  
Vol 35 (3) ◽  
pp. 367 ◽  
Author(s):  
DGDe Marco ◽  
CB Li ◽  
PJ Randall ◽  
Marco DG De
Keyword(s):  
Triticum Aestivum ◽  
Subterranean Clover ◽  
Pasture Legumes ◽  
Relative Tolerance ◽  
Ornithopus Compressus ◽  
Burr Medic ◽  
Lotus Pedunculatus ◽  
Persian Clover ◽  
High Concentrations ◽  
Mn Concentrations

This paper describes the tolerance to high concentrations of manganese (Mn) of pasture legumes that are suitable for the >500 mm rainfall zone in southern Australia. The legumes are lucerne (Medicago sativa), burr medic (M. polymorpha), murex medic (M. murex), balansa clover (Trifolium balansae), Persian clover (T. resupinatum), subterranean clover (T: subterraneum), greater lotus (Lotus pedunculatus), and seradella (Ornithopus compressus). Wheat (Triticum aestivum) cv. Egret and subterranean clover cvv. Mt Barker and Karridale were included to place the tolerance of the remaining species in the context of other studies.Symptoms of toxicity differed between species. Species ranking (in descending order) for Mn tolerance, and external threshold Mn concentrations (mmol/L), were subterranean clover (1.0), wheat (0.71), balansa clover (0.54), greater lotus (0.51), serradella (0.50), Persian clover (0.25), murex medic (0.24), burr medic (0.20), and lucerne (0.19). Critical toxicity concentrations derived from the relationships of yields to Mn concentrations in whole shoots for each species were as follows (mg Mn/kg DW): subterranean clover (2010), balansa clover (1330), serradella (1080), greater lotus (760), wheat (570), burr medic (440), murex medic (430), Persian clover (360), lucerne (190).

Nitrogenase Activity by Subterranean Clover and Other Temperate Pasture Legumes

1988 ◽  
Vol 15 (5) ◽  
pp. 657 ◽  
Author(s):  
AG Davey ◽  
RJ Simpson
Keyword(s):  
Root Respiration ◽  
Nitrogenase Activity ◽  
Subterranean Clover ◽  
Trifolium Subterraneum ◽  
Acetylene Reduction Activity ◽  
Diffusion Resistance ◽  
Pasture Legumes ◽  
Reduction Activity ◽  
Decline Rate ◽  
Nodulated Root

Nitrogenase (C2H2-reduction) activity and nodulated root respiration of intact plants of subterranean clover (Trifolium subterraneum L.) cv. Seaton Park nodulated by Rhizobium trifolii WU95 were measured in a flow-through system. Simultaneous declines in nitrogenase activity and respiration were exhibited 2 min after 10% C2H2 had been introduced into the gas stream. Declines in nitrogenase activity and nodulated root respiration provided an estimate of the efficiency of nitrogenase activity (mol CO2 evolved/mol C2H4 produced). The pre-decline rate of nitrogenase activity at time zero was thus calculated as the product of the respiration associated with nitrogenase activity and the reciprocal of the efficiency of nitrogenase activity. Pre-decline rates of nitrogenase activity were similar to peak rates for several pasture legumes. However, post-decline rates of activity were as much as 70% lower than the pre-decline rate. The age of subterranean clover plants had an important influence on the magnitude of the C2H2-induced decline; young plants exhibited the largest C2H2-induced inhibition of nitrogenase activity. Neither sainfoin (Onobrychis viciifolia Scop.) cv. Othello nodulated by Rhizobium sp. CC1108 nor yellow serradella (Ornithopus compressus L.) cv. Pitman nodulated by R. lupini WU425 exhibited C2H2-induced declines in nitrogenase activity. Nitrogenase-linked respiration of subterranean clover at the 14-leaf stage accounted for 50% of total nodulated root respiration. The oxygen diffusion resistance of the nodules increased in the presence of C2H2 but the effect was reversible once C2H2 was removed from the gas atmosphere. The pre-decline rate of acetylene reduction activity of subterranean clover reached a maximum at 10% C2H2. The C2H2-induced decline in nitrogenase activity was lower at subsaturating pC2H2 and was not detected at 0.4% C2H2.

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