Chemical composition and phytotoxicity of essential oils of Croton doctoris S. Moore (Euphorbiaceae)

Essential oils from the stems and leaves of Croton doctoris were analyzed by gas chromatography and mass spectrometry, resulting in 22 identified compounds. The effects of these essential oils on the germination, root and shoot growth, total chlorophyll content, potential root respiration, peroxidase activity, catalase, superoxide dismutase, and mitotic index in lettuce and onion were determined. Antioxidant, antimicrobial, and cytotoxic activity were also investigated. The results revealed that the stem oil consisted of 15 compounds, of which caryophyllene oxide (24.5%) and E-caryophyllene (13.3%) were the major constituents. The leaf oil contained E-caryophyllene (39.6%) and α-humulene (13.2%) as major compounds. The oils inhibited the germination and growth of lettuce and onion seedlings and reduced chlorophyll content, root respiration, and cell division. They also caused oxidative stress, indicated by the increased activity of the evaluated antioxidant enzymes. These abnormal physiological processes contributed to the inhibition of plant growth. The most pronounced phytotoxic effects were observed in the stem oil. The cytotoxicity tests indicated that leaf oil was more active than stem oil, resulting from the presence of biologically active sesquiterpenes that inhibit the growth of cancer cells.

Responses of fine root exudation, respiration, and morphology in three early successional tree species to increased air humidity and different soil nitrogen sources

Global climate change scenarios predict an increase in air temperature, precipitation, and air humidity for northern latitudes. Elevated air humidity may significantly reduce the water flux through forest canopies and affect interactions between water and nutrient uptake. However, we have limited understanding of how altered transpiration would affect root respiration and carbon (C) exudation as fine root morphology acclimates to different water flux. We investigated the effects of elevated air relative humidity (eRH) and different inorganic nitrogen sources (NO3− and NH4+) on above and belowground traits in hybrid aspen (Populus × wettsteinii Hämet-Ahti), silver birch (Betula pendula Roth.), and Scots pine (Pinus sylvestris L.) grown under controlled climate chamber conditions. The eRH significantly decreased the transpiration flux in all species, decreased root mass-specific exudation in pine, and increased root respiration in aspen. eRH also affected fine root morphology, with specific root area increasing for birch but decreasing in pine. The species comparison revealed that pine had the highest C exudation, while birch had the highest root respiration rate. Both humidity and nitrogen treatments affected the share of absorptive and pioneer roots within fine roots; however, the response was species-specific. The proportion of absorptive roots was highest in birch and aspen, the share of pioneer roots was greatest in aspen, and the share of transport roots was greatest in pine. Fine roots with lower root tissue density were associated with pioneer root tips and had a higher C exudation rate. Our findings underline the importance of considering species-specific differences in relation to air humidity and soil nitrogen availability that interactively affect the C input–output balance. We highlight the role of changes in the fine root functional distribution as an important acclimation mechanism of trees in response to environmental change.

Nitrogen Fertilisation Increases Specific Root Respiration in Ectomycorrhizal but Not in Arbuscular Mycorrhizal Plants: A Meta-Analysis

Plants spend a high proportion of their photosynthetically fixed carbon (C) belowground to support mycorrhizal associations in return for nutrients, but this C expenditure may decrease with increased soil nutrient availability. In this study, we assessed how the effects of nitrogen (N) fertiliser on specific root respiration (SRR) varied among mycorrhizal type (Myco type). We conducted a multi-level meta-analysis across 1,600 observations from 32 publications. SRR increased in ectomycorrhizal (ECM) plants with more than 100 kg N ha−1 applied, did not change in arbuscular mycorrhizal (AM) and non-mycorrhizal (NM) plants, but increased in plants with a dual mycorrhizal association in response to N fertilisation. Our results suggest that high N availability (>100 kg N ha−1) could disadvantage the growth of ECM plants because of increased C costs associated with maintaining higher root N concentrations, while the insensitivity in SRR by AM plants to N fertilisation may be because AM fungi are more important for phosphorus (P) uptake.

Effects of Different Fumigants on the Replanted Soil Environment and Growth of Malus hupehensis Rehd. Seedlings

Apple replant disease (ARD) has been reported in all major fruit-growing regions of the world and is often caused by biotic factors (pathogen fungi) and abiotic factors (phenolic compounds). Soil chemical fumigation can kill soil pathogenic fungi; however, the traditionally used fumigant methyl bromide has been banned because of its ozone-depleting effects. There is thus a need to identify greener fumigant candidates. We characterized the effects of different fumigants on the replanted soil environment and the growth characteristics of Malus hupehensis Rehd. seedlings. All five experimental treatments [treatment 1 (T1), metham-sodium; treatment 2 (T2), dazomet; treatment 3 (T3), calcium cyanamide; treatment 4 (T4), 1,3-dichloropropene; and treatment 5 (T5), methyl bromide] promoted significantly the biomass, root growth, and root respiration rate of M. hupehensis seedlings and the ammonium nitrogen (NH4+-N) and nitrate nitrogen (NO3–-N) contents of replanted soil. Metham sodium (T1) and dazomet (T2) had stronger effects compared with 1,3-dichloropropene (T4) and calcium cyanamide (T3). At 172 days after T1, the height, root length, and root respiration rate of Malus hupehensis Rehd. seedlings, and the NH4+-N and NO3–-N contents of replanted soil increased by 91.64%, 97.67%, 69.78%, 81.98%, and 27.44%, respectively, compared with the control. Thus, dazomet and metham sodium were determined to be the optimal fumigants for use in practical applications.

Carbon allocation in early successional tree species at elevated air humidity and different soil nitrogen sources

<p>Global climate change scenarios predict increasing air temperature, enhanced precipitation and air humidity for Northern latitudes. We investigated the effects of elevated air relative humidity (RH) and different inorganic nitrogen sources (NO<sub>3</sub><sup>-</sup>, NH<sub>4</sub><sup>+</sup>) on above- and belowground traits in different tree species, with particular emphasis on rhizodeposition rates. Silver birch, hybrid aspen and Scots pine saplings were grown in PERCIVAL growth chambers with stabile temperature, light intensity and two different air humidity conditions: moderate (mRH, 65% at day and 80% at night) and elevated (eRH, 80% at day and night). The collection of fine root exudates was conducted by a culture-based cuvette method and total organic carbon content was determined by Vario TOC analyser. Fine root respiration was measured with an infra-red gas analyser CIRAS 2.  </p><p>We analysed species-specific biomass allocation, water and rhizodeposition fluxes, foliar and fine root traits in response to changing environmental conditions. The eRH significantly decreased the transpiration flux in all species. In birch the transpiration flux was also affected by the nitrogen source. The average carbon exudation rate for aspen, birch and pine varied from 2 to 3  μg C g<sup>-1</sup> day <sup>-1</sup>. The exudation rates for deciduous tree species tended to increase at eRH, while conversely decreased for coniferous trees (p=0.045), coinciding with the changes in biomass allocation. C flux released by fine root respiration varied more than the fine root exudation, whereas the highest root respiration was found in silver birch and lowest in aspen. At eRH the above and belowground biomass ratio in aspen increased, at the expense of decreased root biomass and root respiration.  </p><p>Moreover, eRH significantly affected fine root morphology, whereas the response of specific root area was reverse for deciduous and coniferous tree species. However, fine roots with lower root tissue density had higher C exudation rate. Our findings underline the importance of considering species-specific differences by elucidating tree’s acclimation to environmental factors and their interactions.   </p>

Non-invasive imaging of CO2 and O2 concentration reveals hotspots of root respiration

<p>Root respiration constitutes a major contribution to the CO<sub>2</sub> efflux from vegetated soils. Amongst temperature, soil moisture is a key environmental variable determining respiration in soils, because it affects the amount of oxygen available for respiration as well as the CO<sub>2</sub> gas transport within the soil pore space.</p><p>Non-invasive imaging techniques facilitate the in situ observation of the complex respiration patterns in the rhizosphere. We applied planar optodes (80x100 mm²) to map the CO<sub>2</sub> and O<sub>2</sub> concentration in the rhizosphere of white lupine plants (<em>Lupinus albus</em>) grown in slab-shaped glass rhizotrons (150x150x15 mm³) in sandy soil under P-deficient conditions. Respiration was measured daily for 19 days after planting at constant soil moisture content as well as during a drying-rewetting experiment, during which soil volumetric water content varied between 0.1 and 0.3 cm³ cm<sup>-3</sup>.</p><p>During their development, the plants exhibited a heterogeneous spatial pattern of root respiration; the highest CO<sub>2</sub>-concentrations were measured at the root tips and along younger parts of the root system. Heterogeneity in CO<sub>2</sub> and O<sub>2</sub> patterns was most pronounced in the drying-rewetting experiment: Distinct hotspots of CO<sub>2</sub>-release and oxygen consumption emerged 30 to 60 minutes after watering. The hotspot-regions correlated with the location of cluster roots growing close to the optodes, where up to three times increased CO<sub>2</sub> concentrations occurred. Overall CO<sub>2</sub> concentrations in the bulk soil increased as CO<sub>2</sub> accumulated over time as gas diffusion in the wet soil was limited.</p><p>Our results highlight the strong spatial and temporal variability of root respiration throughout the growth and development of the root system, and particularly in response to an increase in soil moisture. Further experiments aim to combine CO<sub>2</sub> and O<sub>2</sub> optode measurements with neutron computed laminography, a tomographic imaging method suited to capture the 3D root system architecture of plants grown in laterally extended rhizotrons in order to link root respiration to root branching order, diameter and functional type.</p>

Impacts of early vegetation on biogeochemical cycles

<p>Lycophytes (club mosses) represent a distinct lineage of vascular plants with a long history including numerous extant and extinct species. They enriched the soil carbon pool through newly developed root-like structures and promoted soil microbial activity by providing organic matter. They enhanced soil carbon dioxide (CO<sub>2</sub>) via root respiration and also modified soil hydrology. These effects had the potential to promote the dissolution of silicate minerals, thus intensifying silicate weathering. The weathering of silicate rocks is considered one of the most significant geo-chemical regulators of atmospheric CO<sub>2</sub> on a long (hundreds of thousands to millions of years) timescale. The motivation for this study is to achieve an increased understanding of the realized impacts of lycophytes on silicate weathering and past climate. To this end, it is necessary to quantify physiological characteristics, spatial distribution, the carbon balance, and hydrological impacts of early lycophytes. These properties, however, cannot be easily derived from proxies. Hence, as a first step, a process-based model is developed here to estimate net carbon uptake by these organisms at the local scale, considering key features such as root distribution, stomatal regulation of water loss, and root respiration.<br>The model features ranges of key physiological traits of lycophytes to predict the emerging characteristics of the lycophyte community under any given climate by implicitly simulating the process of selection. In this way, also extinct plant communities can be represented.<br>In addition to physiological properties, the model also simulates weathering rates using a simple limit-based approach and estimates the biotic enhancement of weathering by lycophytes. We run the Lycophyte model, called LYCOm, at seven sites encompassing various climate zones under today's climatic conditions. LYCOm is able to simulate realistic properties of lycophyte communities at the respective locations and estimates an average NPP ranging from 245 g carbon m<sup>-2</sup> year<sup>-1</sup> in Costa Rica to 126 g carbon m<sup>-2</sup> year<sup>-1</sup> in Estonia. Our limit-based weathering model predicts a chemical weathering rate ranging from 0.026 to 0.31 mm rock a<sup>-1 </sup>, thereby highlighting the potential importance of lycophytes at the local scale for enhancing chemical weathering. Our modeling study establishes a basis for assessing biotic enhancement of weathering by lycophytes at the global scale and also for the geological past. </p>