Epicuticular Wax Structure and Regeneration on Developing Juvenile Eucalyptus Leaves

1996 ◽  
Vol 44 (6) ◽  
pp. 691 ◽  
Author(s):  
MG Wirthensohn ◽  
M Sedgley
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Leaf Age ◽  
Environmental Scanning Electron Microscopy ◽  
Tube Length ◽  
Environmental Scanning ◽  
Eucalyptus Leaves ◽  
Cut Foliage ◽  
Wax Deposits ◽  
Scanning Electron

Eighteen species of Eucalyptus L'Hér. were studied for changes in wax morphology of juvenile foliage with leaf age using environmental scanning electron microscopy. Three species were studied for wax regeneration following removal from the adaxial surface of day 16 and day 30 leaves (16 and 30 days following lamina separation, respectively) while still attached to the tree. For each leaf age, four leaves per species were sampled at 0, 1, 3, 9 and 15 days after wax removal. All species had tube wax on juvenile leaves but there were differences between the species, in the length and conformation of tubes and percentage surface area covered by wax. Tube length was greatest in day 30 leaves, but tube diameter was narrower than day 0 leaves (unfolding leaves). There were significant differences in wax structure between the proximal and distal ends of day 0 leaves of 10 species, and tubes were observed crystallising from amorphous wax deposits in the proximal area only. Significant wax regeneration occurred more rapidly on day 16 leaves than day 30 leaves. Wax morphology was consistent across six species of the series Viminales. Thirteen species of Eucalyptus are recommended for the cut foliage industry.

scholarly journals The Investigation of Silicon Localization and Accumulation in Citrus

Plants ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 200
Author(s):  
Mireille Asanzi Mvondo-She ◽  
Diana Marais
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Leaf Age ◽  
Environmental Scanning Electron Microscopy ◽  
Environmental Scanning ◽  
Outer Cell ◽  
Citrus Species ◽  
Abaxial Leaf Surface ◽  
Scanning Electron

Several studies have demonstrated Si absorption in monocotyledon and dicotyledon species. Regarding Si accumulation, studies in monocotyledons have identified Si deposition around the cell wall, cuticle layer bulliform cells, silica cells and endodermal cells. In previous studies with different citrus species there were evidence of Si accumulation, however no information on Si deposition can be found. Therefore, in this study, Si was applied (0 and 1000 mg L−1) to the roots of two citrus species, ‘Delta’ Valencia (Citrus sinensis) and ‘Nules’ Clementine (Citrus reticulata). Si accumulation were investigated in new, and old leaves and roots. Si deposition was investigated through scanning electron microscopy using energy dispersive analysis X-ray, environmental scanning electron microscopy and light microscopy. Si accumulation was significantly higher in Si treated leaves comparatively to the control in both young and mature leaves. Meanwhile, Si accumulation increased with leaf age. Additionally, Si accumulation was significantly higher in roots than in leaves. With respect to Si deposition, granules were identified in the epidermal cells through SEM and ESEM studies. The light microscopy identified the presence of Si granules in the surface and around the outer cell surface forming the cuticle-silica double layer of the lower epidermis in Si treated plants. Silica deposit were not found on the abaxial leaf surface. The findings suggest a passive uptake in citrus species.

Environmental scanning electron microscopy of fresh human gallstones reveals new morphologies of precipitated calcium salts

1992 ◽  
Vol 50 (2) ◽  
pp. 1322-1323
Author(s):  
Howard S. Kaufman ◽  
Keith D. Lillemoe ◽  
John T. Mastovich ◽  
Henry A. Pitt
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Environmental Scanning Electron Microscopy ◽  
Environmental Scanning ◽  
X Ray Diffraction ◽  
Calcium Salts ◽  
Cholesterol Gallstones ◽  
X Ray ◽  
Ca Carbonate ◽  
Scanning Electron

Gallstones contain precipitated cholesterol, calcium salts, and proteins. Calcium (Ca) bilirubinate, palmitate, phosphate, and carbonate occurring in gallstones have variable morphologies but characteristic windowless energy dispersive x-ray (EDX) spectra. Previous studies of gallstone microstructure and composition using scanning electron microscopy (SEM) with EDX have been limited to dehydrated samples. In this state, Ca bilirubinates appear as either glassy masses, which predominate in black pigment stones, or as clusters, which are found mostly in cholesterol gallstones. The three polymorphs of Ca carbonate, calcite, vaterite, and aragonite, have been identified in gallstones by x-ray diffraction, however; the morphologies of these crystals vary in the literature. The purpose of this experiment was to study fresh gallstones by environmental SEM (ESEM) to determine if dehydration affects gallstone Ca salt morphology.Gallstones and bile were obtained fresh at cholecystectomy from 6 patients. To prevent dehydration, stones were stored in bile at 37°C. All samples were studied within 4 days of procurement.

Characterization of Thin Polymer Blend Films using ESEM – No Charging, No Staining.

2001 ◽  
Vol 707 ◽  
Author(s):  
Ian C. Bache ◽  
Catherine M. Ramsdale ◽  
D. Steve Thomas ◽  
Ana-Claudia Arias ◽  
J. Devin MacKenzie ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Environmental Scanning Electron Microscopy ◽  
Environmental Scanning ◽  
Cross Sectional ◽  
Blend Films ◽  
Thin Polymer ◽  
Device Applications ◽  
Scanning Electron

ABSTRACTCharacterising the morphology of thin films for use in device applications requires the ability to study both the structure within the plane of the film, and also through its thickness. Environmental scanning electron microscopy has proved to be a fruitful technique for the study of such films both because contrast can be seen within the film without the need for staining (as is conventionally done for electron microscopy), and because cross-sectional images can be obtained without charging artefacts. The application of ESEM to a particular blend of relevance to photovoltaics is described.

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