Molecular characterisation of novel LMW-m and LMW-s genes from four Aegilops species (Sitopsis section) and comparison with those from the Glu-B3 locus of common wheat

2016 ◽  
Vol 67 (9) ◽  
pp. 938 ◽  
Author(s):  
Susana Cuesta ◽  
Carlos Guzmán ◽  
Juan B. Alvarez
Keyword(s):  
Common Wheat ◽  
Viscoelastic Properties ◽  
Wheat Breeding ◽  
Low Molecular Weight ◽  
Nucleotide Polymorphisms ◽  
Aegilops Species ◽  
Glutenin Subunits ◽  
Single Nucleotide ◽  
B Genome ◽  
S Genes

Low-molecular-weight glutenin subunits (LMW-GS) are a component of the gluten network and play a key role in determining the viscoelastic properties of wheat dough. Aegilops species have been shown to be an important source of variation for valuable traits for wheat breeding. However, very little is known about LMW-GS genes in section Sitopsis species, which are closely related to the B genome of common wheat. Ten accessions of Sitopsis species were evaluated for variability of LMW-GS genes, and 20 novel genes were obtained, of which nine were LMW-m and 11 were LMW-s genes. Only two were pseudogenes, corresponding to one LMW-m and one LMW-s gene. Six groups of genes were detected: three for each of the LMW-m and LMW-s genes. All groups of LMW-s genes and one of LMW-m genes (pGluU) detected were not related to B-genome genes from common wheat, whereas the remaining genes were. The single-nucleotide polymorphisms, and insertions and deletions, detected in active variants compared with those from common wheat could affect structure protein. The analysis of reactive epitopes for coeliac disease revealed that LMW-s subunits lacked toxicity, as did the pGluU LMW-m subunits; the other LMW-m subunits were less toxic than that from common wheat.

scholarly journals Heteroalleles in Common Wheat: Multiple Differences between Allelic Variants of the Gli-B1 Locus

2021 ◽  
Vol 22 (4) ◽  
pp. 1832
Author(s):  
Eugene Metakovsky ◽  
Laura Pascual ◽  
Patrizia Vaccino ◽  
Viktor Melnik ◽  
Marta Rodriguez-Quijano ◽  
...  
Keyword(s):  
Common Wheat ◽  
Dna Sequences ◽  
Fragment Length Polymorphism ◽  
Snp Markers ◽  
Group Iv ◽  
Nucleotide Polymorphisms ◽  
High Genetic Diversity ◽  
Single Nucleotide ◽  
Allelic Variants ◽  
B Genome

The Gli-B1-encoded γ-gliadins and non-coding γ-gliadin DNA sequences for 15 different alleles of common wheat have been compared using seven tests: electrophoretic mobility (EM) and molecular weight (MW) of the encoded major γ-gliadin, restriction fragment length polymorphism patterns (RFLPs) (three different markers), Gli-B1-γ-gliadin-pseudogene known SNP markers (Single nucleotide polymorphisms) and sequencing the pseudogene GAG56B. It was discovered that encoded γ-gliadins, with contrasting EM, had similar MWs. However, seven allelic variants (designated from I to VII) differed among them in the other six tests: I (alleles Gli-B1i, k, m, o), II (Gli-B1n, q, s), III (Gli-B1b), IV (Gli-B1e, f, g), V (Gli-B1h), VI (Gli-B1d) and VII (Gli-B1a). Allele Gli-B1c (variant VIII) was identical to the alleles from group IV in four of the tests. Some tests might show a fine difference between alleles belonging to the same variant. Our results attest in favor of the independent origin of at least seven variants at the Gli-B1 locus that might originate from deeply diverged genotypes of the donor(s) of the B genome in hexaploid wheat and therefore might be called “heteroallelic”. The donor’s particularities at the Gli-B1 locus might be conserved since that time and decisively contribute to the current high genetic diversity of common wheat.

scholarly journals Proteomic analysis of low-molecular-weight glutenin subunits and relationship with their genes in a common wheat variety

3 Biotech ◽  
2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Hye-Rang Beom ◽  
Jin Sun Kim ◽  
You-Ran Jang ◽  
Sun-Hyung Lim ◽  
Chang-Kug Kim ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Common Wheat ◽  
Proteomic Analysis ◽  
Wheat Variety ◽  
Low Molecular Weight ◽  
Glutenin Subunits ◽  
Common Wheat Variety

Characterization of a wheat mutant missing low-molecular-weight glutenin subunits encoded by the B-genome

2017 ◽  
Vol 73 ◽  
pp. 158-164 ◽  
Author(s):  
Jong-Yeol Lee ◽  
Chon-Sik Kang ◽  
Hye-Rang Beom ◽  
You-Ran Jang ◽  
Susan B. Altenbach ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Low Molecular Weight ◽  
Glutenin Subunits ◽  
B Genome ◽  
Wheat Mutant

scholarly journals Applicability of ‘glume color’ controlled by Rg-B1 locus as a field-marker to detect Glu-B3 alleles encoding low-molecular-weight glutenin subunits in common wheat

2011 ◽  
Vol 61 (1) ◽  
pp. 11-16 ◽  
Author(s):  
Kiyoshi Fujii ◽  
Takako Tsuji ◽  
Tomofumi Yoshida ◽  
Wakako Maruyama-Funatsuki ◽  
Tatsuya M. Ikeda
Keyword(s):  
Molecular Weight ◽  
Common Wheat ◽  
Low Molecular Weight ◽  
Glutenin Subunits

scholarly journals Low-molecular-weight glutenin subunits in common wheat (Triticum aestivum L.)

2014 ◽  
Vol 46 (4) ◽  
pp. 342-352
Author(s):  
Jong-Yeol Lee ◽  
Hye-Rang Beom ◽  
Yeong-Tae Kim ◽  
Sun-Hyung Lim ◽  
Ung-Han Yoon ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Triticum Aestivum ◽  
Common Wheat ◽  
Triticum Aestivum L ◽  
Low Molecular Weight ◽  
Glutenin Subunits

scholarly journals FCoV Viral Sequences of Systemically Infected Healthy Cats Lack Gene Mutations Previously Linked to the Development of FIP

Pathogens ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 603
Author(s):  
Mirjam Lutz ◽  
Aline R. Steiner ◽  
Valentino Cattori ◽  
Regina Hofmann-Lehmann ◽  
Hans Lutz ◽  
...  
Keyword(s):  
Gene Mutations ◽  
Open Reading Frames ◽  
Nucleotide Polymorphisms ◽  
Nonstructural Proteins ◽  
Single Nucleotide ◽  
Systemic Spread ◽  
Viral Sequences ◽  
Highly Virulent ◽  
Reading Frames ◽  
S Genes

Feline Infectious Peritonitis (FIP)—the deadliest infectious disease of young cats in shelters or catteries—is induced by highly virulent feline coronaviruses (FCoVs) emerging in infected hosts after mutations of less virulent FCoVs. Previous studies have shown that some mutations in the open reading frames (ORF) 3c and 7b and the spike (S) gene have implications for the development of FIP, but mainly indirectly, likely also due to their association with systemic spread. The aim of the present study was to determine whether FCoV detected in organs of experimentally FCoV infected healthy cats carry some of these mutations. Viral RNA isolated from different tissues of seven asymptomatic cats infected with the field strains FCoV Zu1 or FCoV Zu3 was sequenced. Deletions in the 3c gene and mutations in the 7b and S genes that have been shown to have implications for the development of FIP were not detected, suggesting that these are not essential for systemic viral dissemination. However, deletions and single nucleotide polymorphisms leading to truncations were detected in all nonstructural proteins. These were found across all analyzed ORFs, but with significantly higher frequency in ORF 7b than ORF 3a. Additionally, a previously unknown homologous recombination site was detected in FCoV Zu1.

Single nucleotide polymorphisms and haplotypes of protein C and protein S genes in the Thai population

2006 ◽  
Vol 17 (1) ◽  
pp. 13-18 ◽  
Author(s):  
Worawan Chumpia ◽  
Chayanon Peerapittayamongkol ◽  
Pantep Angchaisuksiri ◽  
Nantarat Komanasin ◽  
Koichiro Muta ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Protein C ◽  
Protein S ◽  
Nucleotide Polymorphisms ◽  
Thai Population ◽  
Single Nucleotide ◽  
S Genes

scholarly journals A mecA-Negative Strain of Methicillin-Resistant Staphylococcusaureus with High-Level β-Lactam Resistance Contains Mutations in Three Genes

2010 ◽  
Vol 54 (11) ◽  
pp. 4900-4902 ◽  
Author(s):  
Ritu Banerjee ◽  
Michael Gretes ◽  
Christopher Harlem ◽  
Li Basuino ◽  
Henry F. Chambers
Keyword(s):  
Molecular Weight ◽  
Serial Passage ◽  
Low Molecular Weight ◽  
Nucleotide Polymorphisms ◽  
Efflux Transporter ◽  
Penicillin Binding Protein ◽  
Multidrug Efflux ◽  
Genome Resequencing ◽  
Single Nucleotide ◽  
High Level

ABSTRACT We previously generated a ceftobiprole-resistant S taphylococcus aureus strain after high inoculum serial passage of a mec A-negative variant of strain COL (R. Banerjee, M. Gretes, L. Basuino, N. Strynadka, and H. F. Chambers, Antimicrob. Agents Chemother. 52:2089-2096, 2008). Genome resequencing of this strain, CRB, revealed that it differs from its parent by five single-nucleotide polymorphisms in three genes, specifically, those encoding PBP4, a low-molecular-weight penicillin-binding protein, GdpP, a predicted signaling protein, and AcrB, a cation multidrug efflux transporter. CRB displayed resistance to a variety of β-lactams but was hypersusceptible to cefoxitin.

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