Comparison of three selectable marker genes for transformation of wheat by microprojectile bombardment

1998 ◽  
Vol 25 (1) ◽  
pp. 39 ◽  
Author(s):  
Barbara Witrzens ◽  
Richard I.S. Brettell ◽  
Fiona R. Murray ◽  
David McElroy ◽  
Zhongyi Li ◽  
...  
Keyword(s):  
Selectable Marker ◽  
Microprojectile Bombardment ◽  
Single Copy ◽  
Transgenic Wheat ◽  
Immature Embryos ◽  
Marker Genes ◽  
Hygromycin B ◽  
Neomycin Phosphotransferase ◽  
Multiple Copy ◽  
Selectable Marker Genes

Three selectable marker genes were compared for their efficacy in the production of transgenic wheat plants following microprojectile bombardment of cultured immature embryos. While transformed plants were recovered using the bar (phosphinothricin acetyltransferase) gene in combination with bialaphos, and the aphA (neomycin phosphotransferase) gene in combination with geneticin or paromomycin, no transgenic material was obtained with the hpt (hygromycin phosphotransferase) gene and hygromycin B. Southern analysis revealed single copy as well as multiple copy insertions of the bar and aphA transgenes. Inheritance of these selectable marker genes was demonstrated in the T1 generation progenies.

scholarly journals Identification of genes differentially regulated by glucocorticoids and progestins using a Cre/loxP-mediated retroviral promoter-trapping strategy

2002 ◽  
Vol 28 (3) ◽  
pp. 177-192 ◽  
Author(s):  
Y Wan ◽  
SK Nordeen
Keyword(s):  
Target Genes ◽  
Selectable Marker ◽  
Biological Effects ◽  
Fibroblast Cell ◽  
Single Copy ◽  
Mouse Fibroblast ◽  
Marker Genes ◽  
Flanking Sequence ◽  
Selectable Marker Genes ◽  
Promoter Trap

Glucocorticoids and progestins are two classes of steroid hormone with very distinct biological functions. However, the glucocorticoid receptor (GR) and the progesterone receptor (PR) share many structural and functional similarities. One way that glucocorticoids and progestins can exert different biological effects is through their different abilities to regulate the expression of certain target genes. A strategy employing a retroviral promoter-trap and Cre/loxP-mediated site-specific recombination has been developed to identify genes that are differentially regulated by glucocorticoids and progestins. A mouse fibroblast cell line (4F) stably expressing both GR and PR and containing a single copy of a multifunctional selection plasmid is generated. This line is transduced with a self-inactivating retroviral promoter-trap vector carrying coding sequences for Cre-recombinase (Cre) in the U3 region. Integration of the provirus places Cre expression under the control of a genomic flanking sequence. Activation of Cre expression from integration into active genes results in a permanent switch between the selectable marker genes that converts the cells from neomycin-resistant to hygromycin-resistant. Selection for hygromycin resistance after hormone treatment yields recombinants in which Cre sequences in the U3 region are expressed from hormone-inducible upstream cellular promoters. Because Cre-mediated recombination is a permanent event, the expression of the selectable marker genes is independent of ongoing Cre expression. Thus this system permits the identification of genes that are transiently or weakly induced by hormone.

Genetic Engineering with Medicinal Plants: Selection of Fusion Products after Transformation with Dominant Selectable Marker Genes

Planta Medica ◽  
1989 ◽  
Vol 55 (02) ◽  
pp. 231-231
Author(s):  
G. Lurz ◽  
P. Bachmann ◽  
R. Greinwald ◽  
H. Neuhöfer ◽  
F.-C. Czygan
Keyword(s):  
Medicinal Plants ◽  
Genetic Engineering ◽  
Selectable Marker ◽  
Marker Genes ◽  
Selectable Marker Genes ◽  
Dominant Selectable Marker ◽  
Fusion Products ◽  
Selection Of

Heat shock induced excision of selectable marker genes in transgenic banana by the Cre-lox site-specific recombination system

2012 ◽  
Vol 159 (4) ◽  
pp. 265-273 ◽  
Author(s):  
Borys Chong-Pérez ◽  
Rafael G. Kosky ◽  
Maritza Reyes ◽  
Luis Rojas ◽  
Bárbara Ocaña ◽  
...  
Keyword(s):  
Heat Shock ◽  
Selectable Marker ◽  
Marker Genes ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Selectable Marker Genes ◽  
Recombination System ◽  
Transgenic Banana ◽  
Site Specific Recombination System

Positive selectable marker genes for routine plant transformation

2002 ◽  
Vol 38 (2) ◽  
pp. 125-128 ◽  
Author(s):  
Suprasanna Penna ◽  
László Sági ◽  
Rony Swennen
Keyword(s):  
Plant Transformation ◽  
Selectable Marker ◽  
Marker Genes ◽  
Selectable Marker Genes

scholarly journals Erratum: Excision of selectable marker genes from transgenic plants

2002 ◽  
Vol 20 (8) ◽  
pp. 843-843 ◽  
Author(s):  
Peter D. Hare ◽  
Nam-Hai Chua
Keyword(s):  
Transgenic Plants ◽  
Selectable Marker ◽  
Marker Genes ◽  
Selectable Marker Genes

scholarly journals New chimeric promoter useful for expression of selectable marker genes in rice transformation

2005 ◽  
Vol 22 (4) ◽  
pp. 287-294
Author(s):  
Hiromi Higo ◽  
Kayo Tsuruya ◽  
Hironori Mano ◽  
Kana Hasegawa ◽  
Yuzo Minobe
Keyword(s):  
Selectable Marker ◽  
Marker Genes ◽  
Rice Transformation ◽  
Chimeric Promoter ◽  
Selectable Marker Genes

scholarly journals Screening and Functional Verification of Selectable Marker Genes for Cordyceps militaris

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Haiwei Lou ◽  
Yu Zhao ◽  
Renyong Zhao ◽  
Zhiwei Ye ◽  
Junfang Lin ◽  
...  
Keyword(s):  
Homologous Recombination ◽  
Resistance Gene ◽  
Selectable Marker ◽  
Binary Vector ◽  
Marker Gene ◽  
Marker Genes ◽  
Bar Gene ◽  
Selectable Marker Gene ◽  
Glufosinate Ammonium ◽  
Selectable Marker Genes

The selectable marker genes are necessary resistance genes for gene knockout, gene complementation, and gene overexpression in filamentous fungi. Moreover, the more sensitive the filamentous fungi are to antibiotics, the more helpful it is to screen the target transformants. In order to obtain the antibiotic (or herbicide) which can effectively inhibit the growth of Cordyceps militaris and verify the function of the corresponding resistance gene in C. militaris, the sensitivity of C. militaris to hygromycin and glufosinate ammonium was compared to determine the resistance gene that was more suitable for the screening of C. militaris transformants. The binary vector of the selectable marker gene was constructed by combining the double-joint PCR (DJ-PCR) method and the homologous recombination method, and the function of the selectable marker gene in C. militaris was verified by the Agrobacterium tumefaciens-mediated transformation method. The results showed that C. militaris was more sensitive to glufosinate ammonium than hygromycin. The growth of C. militaris could be completely inhibited by 250 μg/mL glufosinate ammonium. The expression cassette of the glufosinate ammonium resistance gene (bar gene) was successfully constructed by DJ-PCR. The binary vector pCAMBIA0390-Bar was successfully constructed by homologous recombination. The bar gene of the vector pCAMBIA0390-Bar was successfully integrated into the C. militaris genome and could be highly expressed in the transformants of C. militaris. This study will promote the identification of C. militaris gene function and reveal the biosynthetic pathways of bioactive components in C. militaris.

Selectable marker genes in transgenic plants: applications, alternatives and biosafety

2004 ◽  
Vol 107 (3) ◽  
pp. 193-232 ◽  
Author(s):  
Brian Miki ◽  
Sylvia McHugh
Keyword(s):  
Transgenic Plants ◽  
Selectable Marker ◽  
Marker Genes ◽  
Selectable Marker Genes

Heat shock induced excision of selectable marker genes in transgenic banana by the Cre-lox site-specific recombination system

2010 ◽  
Vol 150 ◽  
pp. 478-478
Author(s):  
Borys Chong-Pérez ◽  
Geert Angenon ◽  
Rafael G Kosky ◽  
Maritza Reyes ◽  
Luis E. Rojas
Keyword(s):  
Heat Shock ◽  
Selectable Marker ◽  
Marker Genes ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Selectable Marker Genes ◽  
Recombination System ◽  
Transgenic Banana ◽  
Site Specific Recombination System
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