scholarly journals A genome-wide RNA interference screen identifies putative chromatin regulators essential for E2F repression

2007 ◽  
Vol 104 (22) ◽  
pp. 9381-9386 ◽  
Author(s):  
J. Lu ◽  
M.-L. Ruhf ◽  
N. Perrimon ◽  
P. Leder
Keyword(s):  
Rna Interference ◽  
Chromatin Regulators ◽  
Genome Wide ◽  
A Genome

scholarly journals A Genome-Wide RNA Interference Screen Identifies a Differential Role of the Mediator CDK8 Module Subunits for GATA/ RUNX-Activated Transcription in Drosophila

2010 ◽  
Vol 30 (11) ◽  
pp. 2837-2848 ◽  
Author(s):  
Vanessa Gobert ◽  
Dani Osman ◽  
Stéphanie Bras ◽  
Benoit Augé ◽  
Muriel Boube ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Rna Interference ◽  
Cell Fate ◽  
Gata Factor ◽  
Hematopoietic System ◽  
Crystal Cell ◽  
Genome Wide ◽  
A Genome

ABSTRACT Transcription factors of the RUNX and GATA families play key roles in the control of cell fate choice and differentiation, notably in the hematopoietic system. During Drosophila hematopoiesis, the RUNX factor Lozenge and the GATA factor Serpent cooperate to induce crystal cell differentiation. We used Serpent/Lozenge-activated transcription as a paradigm to identify modulators of GATA/RUNX activity by a genome-wide RNA interference screen in cultured Drosophila blood cells. Among the 129 factors identified, several belong to the Mediator complex. Mediator is organized in three modules plus a regulatory “CDK8 module,” composed of Med12, Med13, CycC, and Cdk8, which has long been thought to behave as a single functional entity. Interestingly, our data demonstrate that Med12 and Med13 but not CycC or Cdk8 are essential for Serpent/Lozenge-induced transactivation in cell culture. Furthermore, our in vivo analysis of crystal cell development show that, while the four CDK8 module subunits control the emergence and the proliferation of this lineage, only Med12 and Med13 regulate its differentiation. We thus propose that Med12/Med13 acts as a coactivator for Serpent/Lozenge during crystal cell differentiation independently of CycC/Cdk8. More generally, we suggest that the set of conserved factors identified herein may regulate GATA/RUNX activity in mammals.

scholarly journals A genome-wide RNA interference screen identifies new regulators of androgen receptor function in prostate cancer cells

2013 ◽  
Vol 23 (4) ◽  
pp. 581-591 ◽  
Author(s):  
K. Imberg-Kazdan ◽  
S. Ha ◽  
A. Greenfield ◽  
C. S. Poultney ◽  
R. Bonneau ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Androgen Receptor ◽  
Rna Interference ◽  
Cancer Cells ◽  
Receptor Function ◽  
Prostate Cancer Cells ◽  
Genome Wide ◽  
A Genome ◽  
Androgen Receptor Function

scholarly journals A Genome-wide RNA Interference Screen Reveals that Variant Histones Are Necessary for Replication-Dependent Histone Pre-mRNA Processing

2007 ◽  
Vol 28 (4) ◽  
pp. 692-699 ◽  
Author(s):  
Eric J. Wagner ◽  
Brandon D. Burch ◽  
Ashley C. Godfrey ◽  
Harmony R. Salzler ◽  
Robert J. Duronio ◽  
...  
Keyword(s):  
Rna Interference ◽  
Mrna Processing ◽  
Genome Wide ◽  
A Genome

Chromatin regulators: weaving epigenetic nets

2010 ◽  
Vol 1 (3-4) ◽  
pp. 225-238 ◽  
Author(s):  
Inmaculada Hernández-Muñoz
Keyword(s):  
Nucleosome Position ◽  
Histone Variants ◽  
Cellular Memory ◽  
Intrinsic Signals ◽  
Differentiated Cells ◽  
Chromatin Regulators ◽  
Genome Wide ◽  
Chromatin Regulator ◽  
A Genome ◽  
Multicellular Organisms

AbstractIn multicellular organisms differentiated cells must maintain their cellular memory, which will be faithfully inherited and maintained by their progeny. In addition, these specialized cells are exposed to specific environmental and cell-intrinsic signals and will have to appropriately respond to them. Some of these stimuli lead to changes in a subset of genes or to a genome-wide reprogramming of the cells that will remain after stimuli removal and, in some instances, will be inherited by the daughter cells. The molecular substrate that integrates cellular memory and plasticity is the chromatin, a complex of DNA and histones unique to eukaryotes. The nucleosome is the fundamental unit of the chromatin and nucleosomal organization defines different chromatin conformations. Chromatin regulators affect chromatin conformation and accessibility by covalently modifying the DNA or the histones, substituting histone variants, remodeling the nucleosome position or modulating chromatin looping and folding. These regulators frequently act in multiprotein complexes and highly specific interplays among chromatin marks and different chromatin regulators allow a remarkable array of possibilities. Therefore, chromatin regulator nets act to propagate the conformation of different chromatin regions through DNA replication and mitosis, and to remodel the chromatin fiber to regulate the accessibility of the DNA to transcription factors and to the transcription and repair machineries. Here, the state-of-the-art of the best-known chromatin regulators is reviewed.

scholarly journals A Genome-Wide RNA Interference Screen Identifies a Role for Wnt/β-Catenin Signaling during Rift Valley Fever Virus Infection

2016 ◽  
Vol 90 (16) ◽  
pp. 7084-7097 ◽  
Author(s):  
Brooke Harmon ◽  
Sara W. Bird ◽  
Benjamin R. Schudel ◽  
Anson V. Hatch ◽  
Amy Rasley ◽  
...  
Keyword(s):  
Rna Interference ◽  
Wnt Signaling ◽  
Rift Valley ◽  
Rift Valley Fever ◽  
Rift Valley Fever Virus ◽  
Fever Virus ◽  
Valley Fever ◽  
Rnai Screening ◽  
Genome Wide ◽  
A Genome

ABSTRACTRift Valley fever virus (RVFV) is an arbovirus within theBunyaviridaefamily capable of causing serious morbidity and mortality in humans and livestock. To identify host factors involved in bunyavirus replication, we employed genome-wide RNA interference (RNAi) screening and identified 381 genes whose knockdown reduced infection. The Wnt pathway was the most represented pathway when gene hits were functionally clustered. With further investigation, we found that RVFV infection activated Wnt signaling, was enhanced when Wnt signaling was preactivated, was reduced with knockdown of β-catenin, and was blocked using Wnt signaling inhibitors. Similar results were found using distantly related bunyaviruses La Crosse virus and California encephalitis virus, suggesting a conserved role for Wnt signaling in bunyaviral infection. We propose a model where bunyaviruses activate Wnt-responsive genes to regulate optimal cell cycle conditions needed to promote efficient viral replication. The findings in this study should aid in the design of efficacious host-directed antiviral therapeutics.IMPORTANCERVFV is a mosquito-borne bunyavirus that is endemic to Africa but has demonstrated a capacity for emergence in new territories (e.g., the Arabian Peninsula). As a zoonotic pathogen that primarily affects livestock, RVFV can also cause lethal hemorrhagic fever and encephalitis in humans. Currently, there are no treatments or fully licensed vaccines for this virus. Using high-throughput RNAi screening, we identified canonical Wnt signaling as an important host pathway regulating RVFV infection. The beneficial role of Wnt signaling was observed for RVFV, along with other disparate bunyaviruses, indicating a conserved bunyaviral replication mechanism involving Wnt signaling. These studies supplement our knowledge of the fundamental mechanisms of bunyavirus infection and provide new avenues for countermeasure development against pathogenic bunyaviruses.

scholarly journals Genome-wide decoupling of H2Aub and H3K27me3 in early mouse development

2021 ◽  
Author(s):  
Yezhang Zhu ◽  
Jiali Yu ◽  
Yan Rong ◽  
Yun-Wen Wu ◽  
Heng-Yu Fan ◽  
...  
Keyword(s):  
Polycomb Group ◽  
Mammalian Development ◽  
Mouse Development ◽  
Chromatin Regulators ◽  
Genome Wide ◽  
A Genome ◽  
Independent Functions ◽  
Preimplantation Mouse ◽  
Early Mouse ◽  
Early Mammalian Development

Polycomb group (PcG) proteins are crucial chromatin regulators during development. H2Aub and H3K27me3 are catalyzed by Polycomb-repressive Complex 1 and 2 (PRC1/2) respectively, and largely overlap in the genome due to mutual recruitment of the two complexes. However, whether PRC1/H2Aub and PRC2/H3K27me3 can function independently remains obscure. Here we uncovered a genome-wide decoupling of H2Aub and H3K27me3 in preimplantation mouse embryos, at both canonical PcG targets and broad distal domains. H2Aub represses future bivalent genes without H3K27me3 but does not contribute to maintenance of H3K27me3-dependent non-canonical imprinting. Our study thus revealed their distinct and independent functions in early mammalian development.

Identification of SUMO-dependent chromatin-associated transcriptional repression components by a genome-wide RNA interference screen

2008 ◽  
Vol 2008 (Spring) ◽  
Author(s):  
Guntram Suske ◽  
Bastian Stielow ◽  
Alexandra Sapetschnig ◽  
Imme Krüger ◽  
Michael Boutros
Keyword(s):  
Rna Interference ◽  
Transcriptional Repression ◽  
Genome Wide ◽  
A Genome

scholarly journals Identification of Drosophila Zfh2 as a Mediator of Hypercapnic Immune Regulation by a Genome-Wide RNA Interference Screen

2015 ◽  
Vol 196 (2) ◽  
pp. 655-667 ◽  
Author(s):  
Iiro Taneli Helenius ◽  
Ryan J. Haake ◽  
Yong-Jae Kwon ◽  
Jennifer A. Hu ◽  
Thomas Krupinski ◽  
...  
Keyword(s):  
Rna Interference ◽  
Immune Regulation ◽  
Genome Wide ◽  
A Genome
Sign in / Sign up

Export Citation Format

Share Document