scholarly journals Single Glycosyltransferase, Core 2 β1→6-N-acetylglucosaminyltransferase, Regulates Cell Surface Sialyl-LexExpression Level in Human Pre-B Lymphocytic Leukemia Cell Line KM3 Treated with Phorbolester

1998 ◽  
Vol 273 (41) ◽  
pp. 26779-26789 ◽  
Author(s):  
Mitsuru Nakamura ◽  
Takashi Kudo ◽  
Hisashi Narimatsu ◽  
Yusuke Furukawa ◽  
Jiro Kikuchi ◽  
...  
Keyword(s):  
Cell Surface ◽  
Cell Line ◽  
Leukemia Cell ◽  
Lymphocytic Leukemia ◽  
Leukemia Cell Line ◽  
Lymphocytic Leukemia Cell

scholarly journals Inhibition of Apoptosis in a Human Pre-B–Cell Line by CD23 Is Mediated Via a Novel Receptor

Blood ◽  
1997 ◽  
Vol 90 (1) ◽  
pp. 234-243 ◽  
Author(s):  
Lindsey J. White ◽  
Bradford W. Ozanne ◽  
Pierre Graber ◽  
Jean-Pierre Aubry ◽  
Jean-Yves Bonnefoy ◽  
...  
Keyword(s):  
Cell Line ◽  
Cell Density ◽  
Flow Cytometric Analysis ◽  
Leukemia Cell ◽  
Lymphocytic Leukemia ◽  
Leukemia Cell Line ◽  
Cell Densities ◽  
Lymphocytic Leukemia Cell ◽  
Low Cell Density ◽  
Cells Cultured

Abstract Human CD23 is a 45-kD type II membrane glycoprotein, which functions as a low-affinity receptor for IgE and as a ligand for the CD21 and CD11b/CD11c differentiation antigens. CD23 is released from the surface of cells as soluble fragments, and a 25-kD species of soluble CD23 (sCD23) appears to act as a multifunctional cytokine. In this report, sCD23 is shown to sustain the growth of low cell density cultures of a human pre-B–acute lymphocytic leukemia cell line, SMS-SB: no other cytokine tested was able to induce this effect. Flow cytometric analysis indicates that sCD23 acts to prevent apoptosis of SMS-SB cells. SMS-SB cells cultured at low cell density possess low levels of bcl-2 protein. Addition of sCD23 to cells at low cell density maintained bcl-2 expression at levels equivalent to those observed in SMS-SB cells cultured at higher cell densities. No CD23 mRNA was found in SMS-SB cells, ruling out an autocrine function for CD23 in this cell line model. Although SMS-SB cells do not express the known receptors for CD23, namely CD21, CD11b-CD18, or CD11c-CD18, the cells specifically bind CD23-containing liposomes, but not glycophorin-containing liposomes. Binding of CD23-containing liposomes is inhibited by anti-CD23 but not by anti-CD21 or anti-CD11b/c monoclonal antibodies. The data show that sCD23 prevents apoptosis of the SMS-SB cell line by acting through a novel receptor.

Establishment and Characterization of Acute B-Cell Lymphocytic Leukemia Cell Line Showing (8; 14) and (14; 18) Chromosome Translocation

1990 ◽  
Vol 84 (3) ◽  
pp. 156-161 ◽  
Author(s):  
Hiromitsu Matsuzaki ◽  
Hiroyuki Hata ◽  
Norio Asou ◽  
Hitoshi Suzushima ◽  
Yoshitaka Akahoshi ◽  
...  
Keyword(s):  
B Cell ◽  
Cell Line ◽  
Leukemia Cell ◽  
Chromosome Translocation ◽  
Lymphocytic Leukemia ◽  
Leukemia Cell Line ◽  
Lymphocytic Leukemia Cell

Isolation and structure of cribrostatins 1 and 2 from the blue marine sponge Cribrochalinasp

1992 ◽  
Vol 70 (4) ◽  
pp. 1170-1175 ◽  
Author(s):  
George R. Pettit ◽  
Jeremiah C. Collins ◽  
Delbert L. Herald ◽  
Dennis L. Doubek ◽  
Michael R. Boyd ◽  
...  
Keyword(s):  
Cell Line ◽  
Marine Sponge ◽  
Leukemia Cell ◽  
Lymphocytic Leukemia ◽  
Leukemia Cell Line ◽  
Spectral Studies ◽  
X Ray ◽  
Mass Spectral ◽  
P388 Cell ◽  
Lymphocytic Leukemia Cell

The blue marine sponge cribrostatins 1 (1, 8.8 × 10−6% yield) and 2 (2, 3.1 × 10−6% yield) active against the P388 lymphocytic leukemia cell line (ED50 1.58 and 2.73 μg/mL respectively) have been isolated. The previously known isoquinolinequinones mimosamycin (3), renierone (4), and O-demethylrenierone (5) were also isolated and shown to display activity against the P388 cell line (ED50 0.73, 2.75, and 8.36 μg/mL, respectively). Importantly, both cribrostatin 1 and mimosamycin showed selective activity against all of the nine human melanoma cell lines employed by the U.S. National Cancer Institute. Structural determinations were accomplished utilizing high field nuclear magnetic resonance (400 MHz) and mass spectral studies. Confirmation of the cribrostatin 1 structure was achieved by X-ray crystallographic techniques.

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