scholarly journals Engineering a Glucose-responsive Human Insulin-secreting Cell Line from Islets of Langerhans Isolated from a Patient with Persistent Hyperinsulinemic Hypoglycemia of Infancy

1999 ◽  
Vol 274 (48) ◽  
pp. 34059-34066 ◽  
Author(s):  
Wendy M. MacFarlane ◽  
Joanna C. Chapman ◽  
Ruth M. Shepherd ◽  
Molly N. Hashmi ◽  
Noritaka Kamimura ◽  
...  
Keyword(s):  
Cell Line ◽  
Islets Of Langerhans ◽  
Human Insulin ◽  
Hyperinsulinemic Hypoglycemia ◽  
Secreting Cell

Sulfonylurea receptor 1 and Kir6.2 expression in the novel human insulin-secreting cell line NES2Y

Diabetes ◽  
2000 ◽  
Vol 49 (6) ◽  
pp. 953-960 ◽  
Author(s):  
W. M. Macfarlane ◽  
R. E. O'Brien ◽  
P. D. Barnes ◽  
R. M. Shepherd ◽  
K. E. Cosgrove ◽  
...  
Keyword(s):  
Cell Line ◽  
Human Insulin ◽  
The Novel ◽  
Sulfonylurea Receptor ◽  
Secreting Cell ◽  
Sulfonylurea Receptor 1

Role of tyrosine kinase in insulin release in an insulin secreting cell line (INS-1)

1995 ◽  
Vol 7 (5) ◽  
pp. 505-512 ◽  
Author(s):  
E.J. Verspohl ◽  
B. Tollkühn ◽  
H. Kloss
Keyword(s):  
Tyrosine Kinase ◽  
Cell Line ◽  
Insulin Release ◽  
Secreting Cell

scholarly journals The effects of various hormones and growth factors on the growth of human insulin-producing cell line in serum-free medium

1997 ◽  
Vol 29 (4) ◽  
pp. 209-216 ◽  
Author(s):  
Jae-Jeong Lee ◽  
Jai-Hyun Kwon ◽  
Yong Keun Park ◽  
Ohoak Kwon ◽  
Tai-Wook Yoon
Keyword(s):  
Growth Factors ◽  
Cell Line ◽  
Human Insulin ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free

alpha-Glycerophosphate shuttle in a clonal beta-cell line

1989 ◽  
Vol 256 (1) ◽  
pp. E173-E178 ◽  
Author(s):  
M. D. Meglasson ◽  
K. M. Smith ◽  
D. Nelson ◽  
M. Erecinska
Keyword(s):  
Insulin Secretion ◽  
Cell Line ◽  
Electron Transport Chain ◽  
Direct Evidence ◽  
Mitochondrial Fraction ◽  
Secreting Cell ◽  
Transport Chain ◽  
Beta Cell Line ◽  
Atp Generation ◽  
Hit Cells

It has been proposed that the alpha-glycerophosphate (alpha-GOP) shuttle plays a crucial role in regulation of glycolysis in beta-cells by linking reoxidation of cytosolic NADH to formation of ATP in the electron transport chain (J. Biol. Chem. 265: 8287, 1981). Direct evidence for this suggestion is still lacking, however. In this work the operation of the alpha-GOP shuttle was investigated in the insulin-secreting cell line HIT-T15. The constituent enzymes of the pathway were found to be present in HIT cells. Flavin-linked alpha-GOP dehydrogenase was associated with the mitochondrial fraction, whereas NAD+-dependent alpha-GOP dehydrogenase was localized in the cytosol. In the presence of amobarbital (used to preserve the function of the alpha-GOP shuttle under conditions where oxidation of NADH by the respiratory chain was blocked), glucose increased insulin secretion, O2 consumption, and the cell [ATP]/[ADP] when compared with amobarbital alone. These results indicate that the alpha-GOP shuttle contributes to ATP generation in HIT cells and that its activation may be necessary for the initiation of insulin secretion by glucose.

scholarly journals Pancreatic Transdifferentiation and Glucose-Regulated Production of Human Insulin in the H4IIE Rat Liver Cell Line

2016 ◽  
Vol 17 (4) ◽  
pp. 534 ◽  
Author(s):  
Binhai Ren ◽  
Chang Tao ◽  
Margaret Swan ◽  
Nichole Joachim ◽  
Rosetta Martiniello-Wilks ◽  
...  
Keyword(s):  
Cell Line ◽  
Human Insulin ◽  
Rat Liver ◽  
Liver Cell ◽  
Liver Cell Line

scholarly journals Leptin activates ATP-sensitive potassium channels in the rat insulin-secreting cell line, CRI-G1

1997 ◽  
Vol 504 (3) ◽  
pp. 527-535 ◽  
Author(s):  
J. Harvey ◽  
F. McKenna ◽  
P. S. Herson ◽  
D. Spanswick ◽  
M. L. J. Ashford
Keyword(s):  
Potassium Channels ◽  
Cell Line ◽  
Secreting Cell

Glucose Regulated Production of Human Insulin in Genetically Modified Myoblast Cell Line (C2C12)

2006 ◽  
Vol 21 (6) ◽  
pp. 526
Author(s):  
Seungjoon Oh ◽  
Jeong-Taek Woo ◽  
Sung Woon Kim ◽  
Jin-Woo Kim ◽  
Young Seol Kim
Keyword(s):  
Cell Line ◽  
Human Insulin ◽  
Genetically Modified ◽  
Myoblast Cell Line ◽  
Myoblast Cell

Characterization of a Novel Glucose-Responsive Insulin-Secreting Cell Line, BRIN-BD11, Produced by Electrofusion

Diabetes ◽  
1996 ◽  
Vol 45 (8) ◽  
pp. 1132-1140 ◽  
Author(s):  
N. H. McClenaghan ◽  
C. R. Barnett ◽  
E. Ah-Sing ◽  
Y. H. A. Abdel-Wahab ◽  
F. P. M. O'Harte ◽  
...  
Keyword(s):  
Cell Line ◽  
Secreting Cell
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